1-19. (canceled)
20. A DNA according to any of the following (a) to (1):
(a) a DNA encoding a glycoprotein of Koi herpesvirus (KHV) consisting of the amino acid sequence as shown by SEQ ID NO: 2, 4, 6, 8 or 10;
(b) a DNA encoding a protein consisting of an amino acid sequence wherein one or a few amino acids are deleted, substituted, or added in the amino acid sequence as shown by SEQ ID No: 2, 4, 6, 8 or 10, and having immunogenicity to Koi herpesvirus (KHV);
(c) a DNA encoding a protein consisting of an amino acid sequence being 80% homologous or more with the amino acid sequence as shown by SEQ ID NO: 2, 4, 6, 8 or 10, and having immunogenicity to Koi herpesvirus (KHV):
(d) a DNA consisting of the nucleotide sequence as shown by SEQ ID No: 1, 3, 5, 7 or 9, or its complementary sequence;
(e) a DNA consisting of the nucleotide sequence wherein one or a few nucleotides are deleted, substituted, or added in the nucleotide sequence as shown by SEQ ID NO: 1, 3, 5, 7 or 9, and encoding a protein having immunogenicity to Koi herpesvirus (KHV);
(f) a DNA hybridizing under stringent conditions with a DNA consisting of a sequence complementary to the nucleotide sequence as shown by SEQ ID NO: 1, 3, 5, 7 or 9, and encoding a protein having immunogenicity to Koi herpesvirus (KHV);
(g) a DNA encoding a membrane protein of Koi herpesvirus (KHV) consisting of the amino acid sequence as shown by SEQ ID NO: 12, 14, 16, 18 or 20;
(h) a DNA encoding a protein consisting of an amino acid sequence wherein one or a few amino acids are deleted, substituted, or added in the amino acid sequence as shown by SEQ ID NO: 12, 14, 16, 18 or 20, and having immunogenicity to Koi herpesvirus (KHV);
(i) a DNA encoding a protein consisting of an amino acid sequence being 80% homologous or more with the amino acid sequence as shown by SEQ ID NO: 12, 14, 16, 18 or 20, and having immunogenicity to Koi herpesvirus (KHV);
(j) a DNA consisting of the nucleotide sequence as shown by SEQ ID NO: 11, 13, 15, 17 or 19, or its complementary sequence;
(k) a DNA consisting of a nucleotide sequence wherein one or a few nucleotides are deleted, substituted or added in the nucleotide sequence as shown by SEQ ID NO: 11, 13, 15, 17 or 19, and encoding a protein having immunogenicity to Koi herpesvirus (KHV);
(l) a DNA hybridizing, under stringent conditions, with a DNA consisting of a sequence complementary to the nucleotide sequence as shown by SEQ ID NO: 11, 13, 15, 17 or 19, and encoding a protein having immunogenicity to Koi herpesvirus (KHV).
21. A protein according to any one of the following (m) to (p):
(m) a glycoprotein of Koi herpesvirus (KHV) consisting of the amino acid sequence as shown by SEQ ID NO: 2, 4, 6, 8, or 10;
(n) a protein consisting of an amino acid sequence wherein one or a few amino acids are deleted, substituted, or added in the amino acid sequence as shown by SEQ ID NO: 2, 4, 6, 8, or 10, and having immunogenicity to Koi herpesvirus (KHV);
(o) a membrane protein of Koi herpesvirus (KHV) consisting of the amino acid sequence as shown by SEQ ID NO: 12, 14, 16, 18, or 20;
(p) a protein consisting of an amino acid sequence wherein one or a few amino acids are deleted, substituted or added in the amino acid sequence as shown by SEQ ID NO: 12, 14, 16, 18 or 20, and having immunogenicity to Koi herpesvirus (KHV).
22. A recombinant vector comprising 1 or more DNAs selected from the DNA according to claim 20.
23. A DNA vaccine for carps comprising 1 or more DNAs selected from the DNA according to claim 20.
24. A method for preventing or treating Koi herpesvirus (KHV), wherein the DNA vaccine for carps according to claim 23 is administered to a carp.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A radiation dosimeter for fluid very small substances, comprising a microcapsule which comprises
a radiation transmitting shell body and
a photochromic solution obtained by dissolving a radiation color development tautometric photochromic compound in a solvent and contained in the shell body and which undergoes a change in color when the photochromic solution undergoes a change in color by irradiation of radiation; wherein
a radiation dose and a color change level of the microcapsule have a quantitative relationship; and
a particle diameter showing a peak value in a particle diameter distribution of the microcapsules is adjusted to be substantially the same as a dimension diameter of the fluid very small substance serving as a dose measurement target.
2. A radiation dosimeter for fluid very small substances according to claim 1, wherein the microcapsule has a particle diameter R in a range of 0.5r\u2266R\u22661.5r relative to a dimension diameter r of the fluid very small substance serving as a dose measurement target.
3. A radiation dosimeter for fluid very small substances according to claim 1 or 2, wherein the radiation comprises ultraviolet rays or radioactive rays.
4. A radiation dosimeter for fluid very small substances according to claim 1, wherein the photochromic solution has an irreversible color change property with which the photochromic solution does not decolorize by irradiation of visible rays andor heating after the photochromic solution has undergone a change in color upon the irradiation of radiation.
5. A radiation dosimeter for fluid very small substances according to claim 1, wherein the photochromic solution is a 0.01 to 2 wt % toluene solution of 10-benzoyl-N,N,N\u2032,N\u2032-tetramethyl-10H-phenothiazine-3,7-diamine (BLMB) or a 0.01 to 2 wt % toluene solution of 4,4\u2032,4\u2033-tris(dimethylamino)triphenylmethan (LCV).
6. A radiation dosimeter for fluid very small substances according to claim 1, wherein the radiation transmitting shell body is formed of any one of capsule film materials selected from gelatin, a urea resin, a melamine resin, a urethane resin, and a polyurea resin.
7. A radiation dosimeter for fluid very small substances according to claim 6, wherein the capsule film material is a urea-formaldehyde resin.
8. A radiation dosimeter for fluid very small substances according claim 1, wherein the fluid very small substance is Cryptosporidium which is a pathogenic microorganism having a dimension diameter of 4 to 7 \u03bcm and generated in potable water, and a particle diameter R of the microcapsule is 3 to 8 \u03bcm.
9. A radiation dosimeter for fluid very small substances according to claim 1, wherein the fluid very small substance is Giardia which is a pathogenic microorganism having a dimension diameter of 8 to 12 \u03bcm and generated in potable water, and a particle diameter R of the microcapsule is 6 to 14 \u03bcm.
10. A method of measuring a radiation dose to fluid very small substances, comprising measuring a dose of radiation with which fluid very small substances have been irradiated by a radiation dosimeter in a radiation irradiation device which introduces a fluid into a treatment chamber equipped with a radiation irradiation means and irradiates the fluid with radiation in the treatment chamber by the radiation irradiation means to thereby irradiate the fluid very small substances in the fluid; wherein
the radiation dosimeter comprises a microcapsule which comprises a radiation transmitting shell body and a photochromic solution obtained by dissolving a radiation color development tautometric photochromic compound in a solvent and contained in the shell body;
the microcapsule undergoes a change in color upon a change in color of the photochromic solution by irradiation of radiation;
in the microcapsule, a color change level thereof has a quantitative relationship with a radiation dose; and
measuring a color change level of each radiation dosimeter collected from an outlet of the treatment chamber, the radiation dosimeter being introduced into the treatment chamber of the radiation irradiation device from an inlet port of the treatment chamber, and determining a dose distribution andor a minimum dose of radiation with which each fluid very small substance has been irradiated by the radiation irradiation means.
11. A method of measuring a radiation dose to fluid very small substances according to claim 10, wherein the microcapsule forming the radiation dosimeter has a structure in which a particle diameter showing a peak value in a particle diameter distribution of the microcapsules is adjusted to be substantially the same as a dimension diameter of the fluid very small substance serving as a dose measurement target.
12. A method of measuring a radiation dose to fluid very small substances according to claim 11, wherein the microcapsule forming the radiation dosimeter has a particle diameter R in a range of 0.5r\u2266R\u22661.5r relative to a dimension diameter r of the fluid very small substance serving as a dose measurement target.
13. A method of measuring a radiation dose to fluid very small substances according to any one of claims 10 to 12, wherein
the fluid comprises potable water or a fluid foodstuff;
the radiation comprises ultraviolet rays; and
the fluid very small substance comprises a pathogenic microorganism which is generated in the fluid foodstuff.
14. A method of measuring a radiation dose to fluid very small substances according to claim 10, wherein
the fluid comprises potable water; and
the fluid very small substance comprises Cryptosporidium andor Giardia which areis generated in the potable water.
15. A method of measuring a radiation dose to fluid very small substances according to claim 10, wherein the color change level of the radiation dosimeters collected from the outlet of the treatment chamber is measured for each radiation dosimeter using a flow cytometer.