1. A method of setting a user interface according to user preferences, the method comprising:
requesting user interface element from a first device;
receiving by a second device, the user interface element;
synchronizing user interfaces stored in the second device based on the user interface information which reflects the user preferences; and
storing the user interface information, and
wherein the synchronizing comprises: applying changes in the received user interface element to the user interfaces stored in the second device using an interface identifier.
2. The method of claim 1, wherein the user interface element comprises the interface identifier, a lock that indicates whether the user interface element has been created, updated, or destroyed, and a link that indicates an object address that can be executed by the user interface element.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An isolated spider venom (SV) toxin peptide, comprising a cysteine rich toxin motif, wherein the peptide is a Paracoelotes sp. peptide or a Xysticus acerbus peptide.
2. The isolated SV toxin peptide of claim 1, wherein the cysteine rich toxin motif is a Pasp cysteine rich toxin motif.
3. The isolated SV toxin peptide of claim 1, wherein the cysteine rich toxin motif comprises an amino acid sequence as shown in SEQ ID NO:21.
4. The isolated SV toxin peptide of claim 1, wherein the SV toxin peptide has insecticidal activity.
5. The isolated SV toxin peptide of claim 1, further comprising a signal peptide.
6. The isolated SV toxin peptide of claim 5, wherein the signal peptide is selected from the group consisting of a cuticle signal peptide from Drosophila melanogaster, a chorion signal peptide from Bombyx mori, a apolipophorin signal peptide from Manduca sexta, a sex specific signal peptide from Bombyx mori, a adipokinetic hormone signal peptide from Manduca sexta, a pBMHPC-12 signal peptide from Bombyx mori and a esterase-6 signal peptide from Drosophila melanogaster.
7. The isolated SV toxin peptide of claim 5, comprising an amino acid sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15.
8. An isolated spider venom (SV) toxin peptide, comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and variants having at least 90% identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5.
9. An insecticide composition, comprising a carrier and the spider venom (SV) toxin peptide of any of claims 1-8.
10. An isolated spider venom (SV) toxin nucleic acid, comprising a nucleotide sequence encoding a cysteine rich toxin motif, wherein the nucleic acid is a Paracoelotes sp. nucleic acid or a Xysticus acerbus nucleic acid.
11. The isolated SV toxin nucleic acid of claim 10, wherein the cysteine rich toxin motif is a Pasp cysteine rich toxin motif.
12. The isolated SV toxin nucleic acid of claim 10, wherein the cysteine rich toxin motif comprises an amino acid sequence as shown in SEQ ID NO:21.
13. The isolated SV toxin nucleic acid of claim 10, wherein the nucleic acid encodes a SV toxin peptide having insecticidal activity.
14. The isolated SV toxin nucleic acid of claim 10, further comprising a signal sequence.
15. The isolated SV toxin nucleic acid of claim 14, wherein the signal sequence is selected from the group consisting of a cuticle signal sequence from Drosophila melanogaster, a chorion signal sequence from Bombyx mori, a apolipophorin signal sequence from Manduca sexta, a sex specific signal sequence from Bombyx mori, a adipokinetic hormone signal sequence from Manduca sexta, a pBMHPC-12 signal sequence from Bombyx mori and a esterase-6 signal sequence from Drosophila melanogaster.
16. The isolated SV toxin nucleic acid of claim 14, comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19 and SEQ ID NO:20.
17. An isolated spider venom (SV) toxin nucleic acid, comprising a nucleotide sequence encoding a SV toxin peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and variants thereof having at least 90% identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5.
18. An isolated spider venom (SV) toxin nucleic acid, comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 and variants thereof having at least 90% identity with SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10.
19. An isolated recombinant expression vector, comprising a nucleic acid of any of claims 10-18.
20. The isolated recombinant expression vector of claim 19, wherein the vector is a baculovirus vector.
21. A host cell, comprising the isolated recombinant expression vector of claim 19.
22. A method of expressing an isolated spider venom (SV) toxin peptide, comprising transfecting a recombinant expression vector into a host cell, wherein the vector comprises an isolated spider venom (SV) toxin nucleic acid, and wherein the nucleic acid is a Paracoelotes sp. nucleic acid or a Xysticus acerbus nucleic acid and comprises a nucleotide sequence encoding a cysteine rich toxin motif.
23. The method of claim 22, wherein the cysteine rich toxin motif is a Pasp cysteine rich toxin motif.
24. The method of claim 22, wherein the cysteine rich toxin motif comprises an amino acid sequence as shown in SEQ ID NO:21.
25. The method of claim 22, wherein the SV toxin peptide has insecticidal activity.
26. The method of claim 22, wherein the SV toxin nucleic acid further comprises a signal sequence.
27. The method of claim 26, wherein the signal sequence is selected from the group consisting of a cuticle signal sequence from Drosophila melanogaster, a chorion signal sequence from Bombyx mori, a apolipophorin signal sequence from Manduca sexta, a sex specific signal sequence e from Bombyx mori, a adipokinetic hormone signal sequence e from Manduca sexta, a pBMHPC-12 signal sequence from Bombyx mori and a esterase-6 signal sequence from Drosophila melanogaster.
28. The method of claim 26, wherein the SV toxin peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 and SEQ ID NO:5 and variants having at least 90% identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5.
29. A method of controlling an insect population, comprising contacting an insecticide composition with members of the insect population, wherein the insecticide composition comprises a carrier and a spider venom (SV) toxin peptide, wherein the SV toxin peptide is a Paracoelotes sp. peptide or a Xysticus acerbus peptide and wherein the SV toxin peptide comprises a cysteine rich toxin motif.
30. The method of claim 29, wherein the cysteine rich toxin motif is a Pasp cysteine rich toxin motif.
31. The method of claim 29, wherein the cysteine rich toxin motif comprises an amino acid sequence as shown in SEQ ID NO:21.
32. The method of claim 29, wherein the SV toxin peptide further comprises a signal peptide.
33. The method of claim 32, wherein the signal peptide is selected from the group consisting of a cuticle signal peptide from Drosophila melanogaster, a chorion signal peptide from Bombyx mori, a apolipophorin signal peptide from Manduca sexta, a sex specific signal peptide from Bombyx mori, a adipokinetic hormone signal peptide from Manduca sexta, a pBMHPC-12 signal peptide from Bombyx mori and a esterase-6 signal peptide from Drosophila melanogaster.
34. The method of claim 32, wherein the SV toxin peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15.
35. The method of claim 29, wherein the SV toxin peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and variants having at least 90% identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4 or SEQ ID NO:5.