1. A bioreactor system, as substantially shown and described.
2. A method, as substantially shown and described.
3. Computer readable code, as substantially shown and described.
4. The system of claim 1, further comprising:
a computing system;
and
a bioreactor in operable communication with the computing system, wherein the computing system is operable for controlling the bioreactor system.
5. The method of claim 2, further comprising:
providing a bioreactor;
and
providing an operating system in operable communication with the bioreactor, the operating system in communication with the bioreactor.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A fluorescence measuring method comprising:
a pumping step of irradiating a sample with pulsed pumping light;
a light-detecting step of detecting fluorescence released from said sample pumped with said pumping light; and
a data processing step of subjecting a time waveform of fluorescence detected by said light-detecting step to a data analysis including a fitting calculation in a fitting range acting as a predetermined time range fixedly set for said time waveform of fluorescence so as to compute waveform data;
wherein, in said data processing step,
a time waveform of pumping light determined beforehand and said time waveform of fluorescence are arranged on a time axis used for said fitting calculation such that a fluorescence peak of said time waveform of fluorescence is placed at an initial position earlier or later by a predetermined time width than a time position substantially coinciding with a pumping light peak of said time waveform of pumping light, and
then, while moving said time waveform of fluorescence and fitting range or said time waveform of pumping light with respect to said time axis to an end position on said time axis on the opposite side of said time position where said fluorescence peak substantially coincides with said pumping light peak from said initial position, respective fitting calculations are carried out at a plurality of time positions different from each other with reference to said time waveform of pumping light, and
a waveform data item selected according to a predetermined selection criterion from a plurality of waveform data items respectively computed in said fitting calculations is employed as final measurement waveform data.
2. A fluorescence measuring method according to claim 1, wherein, in said data processing step, one of said time waveform of pumping light and said time waveform of fluorescence is arranged at a fixed time position on said time axis, and the other is moved with respect to said time axis.
3. A fluorescence measuring method according to claim 1, wherein, in said data processing step, a fluorescence lifetime is determined according to said measurement waveform data.
4. A fluorescence measuring method according to claim 1, wherein, in said data processing step, 2 values respectively determined in said fitting calculations are used as said selection criterion, and said waveform data computed in said fitting calculation yielding the minimal 2 value is selected as said measurement waveform data.
5. A fluorescence measuring method according to claim 1, wherein, in said data processing step, a time range set with reference to said fluorescence peak of said time waveform of fluorescence is used as said fitting range.
6. A fluorescence measuring method according to claim 1, further comprising a range setting step of setting said fitting range prior to said pumping step.
7. A fluorescence measuring apparatus comprising:
pumping means for irradiating a sample with pulsed pumping light;
light-detecting means for detecting fluorescence released from said sample pumped with said pumping light; and
data processing means for subjecting a time waveform of fluorescence detected by said light-detecting means to a data analysis including a fitting calculation in a fitting range acting as a predetermined time range fixedly set for said time waveform of fluorescence so as to compute waveform data;
wherein said data processing means arranges a time waveform of pumping light determined beforehand and said time waveform of fluorescence on a time axis used for said fitting calculation such that a fluorescence peak of said time waveform of fluorescence is placed at an initial position earlier or later by a predetermined time width than a time position substantially coinciding with a pumping light peak of said time waveform of pumping light, and
then, while moving said time waveform of fluorescence and fitting range or said time waveform of pumping light with respect to said time axis to an end position on said time axis on the opposite side of said time position where said fluorescence peak substantially coincides with said pumping light peak from said initial position, carries out respective fitting calculations at a plurality of time positions different from each other with reference to said time waveform of pumping light, and
employs a waveform data item selected according to a predetermined selection criterion from a plurality of waveform data items respectively computed in said fitting calculations as final measurement waveform data.
8. A fluorescence measuring apparatus according to claim 7, wherein said data processing means arranges one of said time waveform of pumping light and said time waveform of fluorescence at a fixed time position on said time axis and moves the other with respect to said time axis.
9. A fluorescence measuring apparatus according to claim 7, wherein said data processing means determines a fluorescence lifetime according to said measurement waveform data.
10. A fluorescence measuring apparatus according to claim 7, wherein said data processing means uses 2 values respectively determined in said fitting calculations as said selection criterion, and selects said waveform data computed in said fitting calculation yielding the minimal 2 value as said measurement waveform data.
11. A fluorescence measuring apparatus according to claim 7, wherein said data processing means uses a time range set with reference to said fluorescence peak of said time waveform of fluorescence as said fitting range.
12. A fluorescence measuring apparatus according to claim 7, further comprising range setting means for setting said fitting range beforehand.
13. A sample evaluating apparatus comprising:
the fluorescence measuring apparatus according to claim 7; and
sample evaluating means for evaluating said sample by comparing said measurement waveform data obtained in said data processing means of said fluorescence measuring apparatus and reference waveform data determined beforehand with each other.