1. A two-color molding equipment for shaping a two-color molded product by placing a film in primary mold tool configured by a fixed mold and a movable mold, injecting a resin into a first cavity formed by closing the molds to shape a primary molded piece, shifting the primary molded piece provided with the film to secondary mold tool configured by the fixed mold and the movable mold, and injecting a resin into a second cavity formed by closing the molds to shape a secondary molded piece, the two-color molded product composed of an integrated combination of the primary molded piece, the secondary molded piece, and the film interposed therebetween, wherein
the first cavity is configured to shape the primary molded piece to be smaller than the film so that a part of the film protrudes from the primary molded piece, and
a wall surface located at the protruding portion of the film to constitute the first cavity is provided with a convex portion that restricts mold shrinkage of the film protruding portion in the primary molded piece.
2. The two-color molding equipment according to claim 1, wherein the first cavity has a rectangular shape when seen from a direction of closing the tools, the convex portion is provided at respective ends with concave portions, and the concave portions are located at both ends of the first cavity forming wall surface.
3. The two-color molding equipment according to claim 1, wherein the second cavity is sized so as to shape the secondary molded piece that covers substantially an entire surface of the film.
4. The two-color molding equipment according to claim 3, wherein the film is prepared as a functional film that is provided with an electric circuit.
5. The two-color molding equipment according to claim 4, wherein the electric circuit is provided on a surface opposite to a surface on which the secondary molded piece is overlaid.
6. The two-color molding equipment according to claim 2, wherein the second cavity is sized so as to shape the secondary molded piece that covers substantially an entire surface of the film.
7. The two-color molding equipment according to claim 6, wherein the film is prepared as a functional film that is provided with an electric circuit or the like.
8. The two-color molding equipment according to claim 7, wherein the electric circuit is provided on a surface opposite to a surface on which the secondary molded piece is overlaid.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A seed of maize inbred line designated PHCA5, representative seed of said line having been deposited under ATCC Accession No: PTA-XXXX.
2. A maize plant, or a part thereof, produced by growing the seed of claim 1.
3. Pollen of the plant of claim 2.
4. An ovule or ovules of the plant of claim 2.
5. The maize plant of claim 2, wherein said plant has been detasseled.
6. A maize plant, or a part thereof, expressing all the physiological and morphological characteristics of inbred line PHCA5, representative seed of said line having been deposited under ATCC Accession No: PTA-XXXX.
7. A maize plant cell comprising all of the alleles of inbred line PHCA5 at the SSR loci listed in Table 2, representative seed of said line having been deposited under ATCC Accession No: PTA-XXXX.
8. A maize seed comprising the maize plant cell of claim 7.
9. A maize plant, or a part thereof, produced by growing the seed of claim 8.
10. A tissue culture comprising the maize plant cell of claim 7.
11. A tissue culture of regenerable cells produced from the plant of claim 2.
12. Protoplasts or callus produced from the tissue culture of claim 11.
13. The tissue culture of claim 11, wherein the regenerable cells of the tissue culture are produced from protoplasts or from tissue of a plant part selected from the group consisting of leaf, pollen, embryo, immature embryo, meristematic cells, immature tassels, microspores, root, root tip, anther, silk, flower, kernel, ear, cob, husk and stalk.
14. A maize plant regenerated from the tissue culture of claim 13, said plant having all the morphological and physiological characteristics of maize inbred line PHCA5, representative seed of said line having been deposited under ATCC Accession No: PTA-XXXX.
15. A process for producing an F1 hybrid maize seed, said process comprising crossing the plant of claim 2 with a different maize plant and harvesting F1 hybrid maize seed.
16. The process of claim 16, further comprising growing the F1 hybrid maize seed to produce a hybrid maize plant.
17. A process of producing a maize plant with a desired trait, wherein the desired trait is selected from the group consisting of male sterility, site-specific recombination, and abiotic stress tolerance, comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers said desired trait.
18. A maize plant produced by the process of claim 17, wherein the desired trait is male sterility and the trait is conferred by a nucleic acid molecule that confers male sterility.
19. A maize plant produced by the method of claim 17, wherein the desired trait is site-specific recombination and the site-specific recombination site is conferred by a member of the group consisting of flpfrt, crelox, Gin, Pin, and RRS.
20. A maize plant produced by the process of claim 17, wherein the desired trait is abiotic stress tolerance and the trait is conferred by a member of the group consisting of cytokinin, ethylene, abscisic acid, CBF, and a transcription factor.
21. A process of producing a herbicide resistant, disease resistant or insect resistant maize plant comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers said resistance.
22. A maize plant produced by the process of claim 21, wherein said resistance comprises herbicide, disease or insect resistance.
23. The resistant maize plant of claim 22, wherein said resistance is herbicide resistance and the herbicide is glyphosate, glufosinate, a sulfonylurea herbicide, an imidazolinone herbicide, a hydroxyphenylpyruvate dioxygenase inhibitor or a protoporphyrinogen oxidase inhibitor.
24. The resistant maize plant of claim 22, wherein the resistance is insect resistance and the insect resistance is conferred by a nucleic acid molecule encoding a Bacillus thuringiensis endotoxin.
25. The resistant maize plant of claim 22, wherein the resistance is disease resistance and the disease is caused by a bacterium, fungus, nematode or virus.
26. A process of producing a maize plant with altered grain characteristics, comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers altered phosphorus, antioxidants, fatty acids, essential amino acids or carbohydrates.
27. A maize plant produced by the process of claim 26, wherein said altered grain characteristics are conferred by a nucleic acid molecule selected from the group consisting of thioredoxin, dek1, Ipa1, Ipa3, mi1ps, hpt and hggt.
28. A process of introducing a desired trait into maize inbred line PHCA5 comprising:
(a) crossing PHCA5 plants grown from PHCA5 seed, representative seed of which has been deposited under ATCC Accession No: PTA-XXXX, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, site-specific recombination, increased transformability and abiotic stress tolerance;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the PHCA5 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PHCA5 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
29. A plant produced by the process of claim 28, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
30. The plant of claim 29, wherein the desired trait is male sterility and the trait is conferred by a nucleic acid molecule that confers male sterility.
31. The plant of claim 29, wherein the desired trait is site specific recombination and the site-specific recombination site is conferred by a member of the group consisting of flpfrt, crelox, Gin, Pin, and RRS.
32. The plant of claim 29, wherein the desired trait is abiotic stress tolerance and the trait is conferred by a member of the group consisting of cytokinin, ethylene, abscisic acid, CBF, and a transcription factor.
33. The plant of claim 29, wherein the desired trait is increased transformability and the trait is conferred by inbred maize line Hi-II.
34. A process of introducing a desired trait into maize inbred line PHCA5 comprising:
(a) crossing PHCA5 plants grown from PHCA5 seed, representative seed of which has been deposited under ATCC Accession No: PTA-XXXX, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of herbicide resistance, insect resistance and disease resistance;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the PHCA5 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PHCA5 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
35. A plant produced by the process of claim 34, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
36. The plant of claim 35, wherein the desired trait is herbicide resistance and said herbicide is glyphosate, glufosinate, a sulfonylurea herbicide, an imidazolinone herbicide, a hydroxyphenylpyruvate dioxygenase inhibitor or a protoporphyrinogen oxidase inhibitor.
37. The plant of claim 35, wherein the desired trait is insect resistance and the insect resistance is conferred by a nucleic acid molecule encoding a Bacillus thuringiensis endotoxin.
38. The plant of claim 35, wherein the desired trait is disease resistance and the disease is caused by a bacterium, fungus, nematode or virus.
39. A process of introducing altered grain characteristics trait into maize inbred line PHCA5 comprising:
(a) crossing PHCA5 plants grown from PHCA5 seed, representative seed of which has been deposited under ATCC Accession No: PTA-XXXX, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is altered phosphorus, antioxidants, fatty acids, essential amino acids or carbohydrates;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the PHCA5 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PHCA5 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
40. A plant produced by the process of claim 39, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PHCA5 listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.
41. The plant of claim 40, wherein the desired trait is altered carbohydrate and the carbohydrate is waxy starch.