1. A method of marking a petroleum product comprising adding a covert dye to the petroleum product, wherein the covert dye is selected from the group consisting of boron azadipyrromethene dyes, boron dipyrromethene dyes, and any combination thereof.
2. A method of identifying a petroleum product comprising spectroscopically analyzing a portion of the petroleum product for the presence of a boron azadipyrromethene dye, a boron dipyrromethene dye, or a combination thereof.
3. A method of detecting a counterfeit or adulterated petroleum product, the method comprising:
spectroscopically analyzing a portion of the petroleum product for the presence of a boron azadipyrromethene dye, a boron dipyrromethene dye, or a combination thereof;
determining a concentration of at least one boron azadipyrromethene or boron dipyrromethene dye present in the portion of the petroleum product;
comparing the determined concentration with a target concentration; and
identifying the petroleum product as counterfeit, adulterated, or authentic based on the determined concentration of the boron azadipyrromethene or boron dipyrromethene dye.
4. The method of any of the above claims, wherein the petroleum product comprises an oxygenate.
5. The method of claim 4, wherein the oxygenate is selected from the group consisting of methanol, ethanol, gasoline grade t-butanol, methyl t-butyl ether, and any combination thereof.
6. A marked petroleum product comprising a mixture of a fluid petroleum product and a dye selected from the group consisting of boron azadipyrromethene dyes, boron dipyrromethene dyes, or any combination thereof.
7. The method of any of the above claims, wherein the petroleum product is selected from the group consisting of gasoline, diesel fuel, biodiesel fuel, kerosene, liquefied petroleum gas, ethanol, industrial solvents, and any combination thereof.
8. A method of detecting an adulterated petroleum product comprising spectroscopically analyzing a portion of the petroleum product for a presence of a marker, wherein the presence of a boron azadipyrromethene dye or a boron dipyrromethene dye is indicative of adulteration of the petroleum product.
9. A method of detecting an adulterated petroleum product, the method comprising:
mixing a boron azadipyrromethene dye, a boron dipyrromethene dye, or any combination thereof with a substance; and
spectroscopically analyzing a portion of a petroleum product for a presence of the boron azadipyrromethene dye or the boron dipyrromethene dye, wherein the presence of the boron azadipyrromethene dye or the boron dipyrromethene dye is indicative of adulteration of the petroleum product with the substance.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An isolated nucleic acid molecule comprising a polymorphic site at position 11646 of SEQ ID NO: 1, and at least 8 contiguous nucleotides of SEQ ID NO: 1 adjacent to the polymorphic site, wherein the nucleic acid molecule comprises a guanine at the position; or a nucleic acid molecule that is fully complementary to the nucleic acid molecule.
2. A nucleic acid molecule according to claim 1, which comprises at least 15 contiguous bases of SEQ ID NO: 1 adjacent to the polymorphic site.
3. A nucleic acid molecule according to claim 1, which comprises at least 20 contiguous bases of SEQ ID NO: 1 adjacent to the polymorphic site.
4. An isolated nucleic acid molecule according to claim 1, which comprises not more than 150 nt.
5. An isolated nucleic acid molecule according to claim 1, which comprises not more than 100 nt.
6. An isolated nucleic acid molecule according to claim 1, which comprises not more than 50 nt.
7. A nucleic acid molecule according to claim 1, wherein the polymorphic site is within 4 nucleotides of the center of the nucleic acid molecule.
8. A nucleic acid molecule according to claim 7, wherein the polymorphic site is at the center of the nucleic acid molecule.
9. A nucleic acid molecule according to claim 1, wherein the polymorphic site is at the 3\u2032-end of the nucleic acid molecule.
10. An array of nucleic acid molecules comprising at least two nucleic acid molecules according to claim 1.
11. A kit comprising a nucleic acid molecule of claim 1, and a suitable container.
12. A method for detecting single nucleotide polymorphism in bovine FGF2 gene, wherein the FGF2 gene have a nucleic acid sequence of SEQ ID NO: 1, the method comprising determining the identity of a nucleotide of position 11646 of the FGF2 gene, and comparing the nucleotide identity of the position respectively to the nucleotide identity at a corresponding position of SEQ ID NO: 1.
13. A method for genotyping a bovine cell, comprising obtaining a nucleic acid sample from said cell and determining the identity of a nucleotide at position 11646 of the FGF2 gene according to claim 12.
14. A method according to claim 13, wherein the bovine cell is an adult cell, an embryo cell, a sperm, an egg, a fertilized egg, or a zygote.
15. A method according to claim 13, wherein the identity of the nucleotide is determined by sequencing the FGF2 gene, or a relevant fragment thereof, isolated from the cell.
16. A method according to claim 14, wherein the gene or a relevant fragment thereof is isolated from the cell via amplification by the polymerase chain reaction (PCR) of genomic DNA of the cell, or by RT-PCR of the mRNA of the cell.
17. A method according to claim 15, wherein both copies of the gene in the cell are genotyped.
18. A method for progeny testing of cattle, the method comprising collecting a nucleic acid sample from said progeny, and genotyping said nucleic sample according to claim 13.
19. A method for selectively breeding of cattle using a multiple ovulation and embryo transfer procedure (MOET), the method comprising superovulating a female animal, collecting eggs from said superovulated female, in vitro fertilizing said eggs from a suitable male animal, implanting said fertilized eggs into other females allowing for an embryo to develop, and genotyping said developing embryo according to claim 13, and terminating pregnancy if the developing embryo does not have guanine at position 11646 of the FGF2 gene.
20. A method for selectively breeding dairy cattle, comprising selecting a bull that is homozygously guanine at position 11646 of the FGF2 gene, and using its semen for fertilizing a female animal.
21. A method according to claim 20, wherein the female animal is in vitro fertilized.
22. The method according to claim 20, wherein the bull is homozygously guanine at position 11646.
23. A method according to claim 20, wherein MOET procedure is used.
24. A method according to claim 22, wherein said female animal is also homozygously guanine at position 11646 of the FGF2 gene.
25. A method for testing a dairy cattle for its fertilization rate or embryo survival rate, or both, comprising genotyping its cells according to claim 13, wherein a cattle homozygously have guanine at position 11646 of the FGF2 gene indicates that the cattle has desirable fertilization rate or embryonic survival rate.