1. A process for cultivating animal cells producing complex proteins, wherein one plant-derived peptone or a combination of plant-derived peptones is fed to a cell culture.
2. The process of claim 1, wherein a basal medium is used for cell inoculation and a feed medium is fed to the cell culture.
3. The process of claim 1, wherein the process is a fed batch process.
4. The process of claim 1, wherein the process is a fed perfusion process.
5. The process of claim 1, wherein the cultivated cells are secreting proteins.
6. The process of claim 5, wherein the secreted proteins are antibodies.
7. The process of claim 1, wherein feeding of the peptone or combination of peptones is started before the cell culture reaches stationary phase.
8. The process of claim 1, wherein feeding of the peptone or combination of peptones is started when the cell culture has reached stationary phase.
9. The process of claim 1, wherein feeding of the peptone or combination of peptones is started at any time between cell inoculation and before cell viability decreases below viability at cell inoculation.
10. The process of claim 9, wherein feeding of the peptone or combination of peptones is started at any time between cell inoculation and three days before cell viability decreases below viability at cell inoculation.
11. The process of claim 10, wherein feeding of the peptone or combination of peptones is started at any time between cell inoculation and three days before cell viability decreases below viability at cell inoculation.
12. The process of claim 1, wherein feeding of the peptone or combination of peptones is started at any time between cell inoculation and before cell viability begins to decrease.
13. The process of claim 1, wherein the peptone or combination of peptones is fed when the viable cell density and or the cell viability has begun to decrease.
14. The process of claim 1, wherein one peptone is fed to the cell culture.
15. The process of claim 14, wherein the peptone is derived from Fabaceae vicieae protein.
16. The process of claim 1, wherein a combination of peptones is fed to the cell culture.
17. The process of claim 16, wherein the combination of peptones comprises a peptone produced by enzymatic digest and which is derived from protein of the Fabaceae family vicieae tribe.
18. The process of claim 17, wherein the peptone is derived from Pisum sativum (pea).
19. The process of claim 18, wherein the combination of peptones further comprises peptones derived from Fabaceae glycine max protein (soy), or Malvaceae seed protein, or both.
20. The process of claim 19, wherein the peptone derived from the seed protein of a Malvaceae is Malvaceae gossypium (cotton seed protein).
21. The process of claim 1, wherein the total dose for the process corresponds to a total concentration of peptone or combination of peptones fed to the cell culture from and including 0.01 gram per litre of cultivation volume at inoculation to and including 15 gram per litre of cultivation volume at inoculation.
22. The process of claim 21, wherein the total dose for the process corresponds to a total concentration of peptone or combination of peptones fed to the cell culture from and including 0.01 gram per litre of cultivation volume at inoculation to and including 11 gram per litre of cultivation volume at inoculation.
23. The process of claim 22, wherein the total dose for the process corresponds to a total concentration of peptone or combination of peptones fed to the cell culture from and including 0.01 gram per litre of cultivation volume at inoculation to and including 5 gram per litre of cultivation volume at inoculation.
24. The process of claim 1, wherein the animal cells are mammalian cells.
25. The process of claim 24, wherein the mammalian cells are human cells or rodent cells.
26. The process of claim 25, wherein the rodent cells are hamster cells.
27. The process of claim 26, wherein the hamster cells are Chinese Hamster Ovary cells.
28. The process of claim 1, wherein the feed medium is added continuously to the cell culture.
29. The process of claim 1, wherein the feed medium is added intermittently to the cell culture.
30. The process of claim 1, wherein the feed medium is added boost-wise to the cell culture.
31. The process of claim 1, wherein the basal medium contains peptones.
32. A method for reducing the toxic effect of over-feeding amino acids during a fed-batch process for cultivating animal cells producing complex proteins, by feeding one plant-derived peptone or a combination of plant-derived peptones to the cell culture according to the process of claim 1.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An electric apparatus comprising:
sensor means (18) for detecting objects (34, 36) in the proximity of the apparatus (10), and
a directional pointing unit (20) which can be directed onto objects (34, 36) in the proximity of the apparatus (10).
2. An apparatus as claimed in claim 1, comprising:
at least one memory (M) for storing the position (\u03b1, \u03b2) of objects (34, 36).
3. An apparatus as claimed in claim 1, wherein
the pointing unit comprises a mechanical pointing element which is mechanically movable in such a way that it can be directed onto objects in the proximity of the apparatus.
4. An apparatus as claimed in claim 1, wherein
the pointing unit (20) comprises a light source for generating a concentrated light beam (40), and
means for directing the light beam (40) onto objects (34, 36) in the proximity of the apparatus (10).
5. An apparatus as claimed in claim 4, wherein
the light source is mechanically movable.
6. An apparatus as claimed in claim 4, wherein
means for directing the light beam (40) comprise one or more mechanically movable mirrors.
7. An apparatus as claimed in claim 1, comprising
a personification element (14) having a front side (16),
motion means for mechanically moving the personification element (14),
means for determining the position of a user, and
control means which are constituted in such a way that they control the motion means in such a way that the front side (16) of the personification element (14) is directed towards the user’s position.
8. An apparatus as claimed in claim 7, wherein the pointing unit (20) is arranged on the personification element (14).
9. An apparatus as claimed in claim 1, comprising
means for speech recognition and speech output.
10. A method of communication between an apparatus (10) and a user, wherein
the apparatus (10) detects objects (34, 36) in its proximity by way of sensor means (18), and
stores the position of objects (34, 36) in a memory (M), and aligns a directional pointing unit (10) with one of the objects (36).