What is claimed is:
1. An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-10, a mature protein coding portion of SEQ ID NO: 1-10, an active domain of SEQ ID NO: 1-10, and complementary sequences thereof.
2. An isolated polynucleotide encoding a polypeptide with biological activity, wherein said polynucleotide hybridizes to the polynucleotide of claim 1 under stringent hybridization conditions.
3. An isolated polynucleotide encoding a polypeptide with biological activity, wherein said polynucleotide has greater than about 90% sequence identity with the polynucleotide of claim 1.
4. The polynucleotide of claim 1 wherein said polynucleotide is DNA.
5. An isolated polynucleotide of claim 1 wherein said polynucleotide comprises the complementary sequences.
6. A vector comprising the polynucleotide of claim 1.
7. An expression vector comprising the polynucleotide of claim 1.
8. A host cell genetically engineered to comprise the polynucleotide of claim 1.
9. A host cell genetically engineered to comprise the polynucleotide of claim 1 operatively associated with a regulatory sequence that modulates expression of the polynucleotide in the host cell.
10. An isolated polypeptide, wherein the polypeptide is selected from the group consisting of:
a) a polypeptide encoded by any one of the polynucleotides of claim 1; and
b) a polypeptide encoded by a polynucleotide hybridizing under stringent conditions with any one of SEQ ID NO: 1-10.
11. A composition comprising the polypeptide of claim 10 and a carrier.
12. An antibody directed against the polypeptide of claim 10.
13. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample with a compound that binds to and forms a complex with the polynucleotide of claim 1 for a period sufficient to form the complex; and
b) detecting the complex, so that if a complex is detected, the polynucleotide of claim 1 is detected.
14. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample under stringent hybridization conditions with nucleic acid primers that anneal to the polynucleotide of claim 1 under such conditions;
b) amplifying a product comprising at least a portion of the polynucleotide of claim 1; and
c) detecting said product and thereby the polynucleotide of claim 1 in the sample.
15. The method of claim 14, wherein the polynucleotide is an RNA molecule and the method further comprises reverse transcribing an annealed RNA molecule into a cDNA polynucleotide.
16. A method for detecting the polypeptide of claim 10 in a sample, comprising:
a) contacting the sample with a compound that binds to and forms a complex with the polypeptide under conditions and for a period sufficient to form the complex; and
b) detecting formation of the complex, so that if a complex formation is detected, the polypeptide of claim 10 is detected.
17. A method for identifying a compound that binds to the polypeptide of claim 10, comprising:
a) contacting the compound with the polypeptide of claim 10 under conditions sufficient to form a polypeptidecompound complex; and
b) detecting the complex, so that if the polypeptidecompound complex is detected, a compound that binds to the polypeptide of claim 10 is identified.
18. A method for identifying a compound that binds to the polypeptide of claim 10, comprising:
a) contacting the compound with the polypeptide of claim 10, in a cell, under conditions sufficient to form a polypeptidecompound complex, wherein the complex drives expression of a reporter gene sequence in the cell; and
b) detecting the complex by detecting reporter gene sequence expression, so that if the polypeptidecompound complex is detected, a compound that binds to the polypeptide of claim 10 is identified.
19. A method of producing the polypeptide of claim 10, comprising,
a) culturing a host cell comprising a polynucleotide sequence selected from the group consisting of a polynucleotide sequence of SEQ ID NO: 1-10, a mature protein coding portion of SEQ ID NO: 1-10 an active domain of SEQ ID NO: 1-10, complementary sequences thereof and a polynucleotide sequence hybridizing under stringent conditions to SEQ ID NO: 1-10, under conditions sufficient to express the polypeptide in said cell; and
b) isolating the polypeptide from the cell culture or cells of step (a).
20. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of any one of the polypeptides from the Sequence Listing, the mature protein portion thereof, or the active domain thereof.
21. The polypeptide of claim 20 wherein the polypeptide is provided on a polypeptide array.
22. A collection of polynucleotides, wherein the collection comprising the sequence information of at least one of SEQ ID NO: 1-10.
23. The collection of claim 22, wherein the collection is provided on a nucleic acid array.
24. The collection of claim 23, wherein the array detects full-matches to any one of the polynucleotides in the collection.
25. The collection of claim 23, wherein the array detects mismatches to any one of the polynucleotides in the collection.
26. The collection of claim 22, wherein the collection is provided in a computer-readable format.
27. A method of treatment comprising administering to a mammalian subject in need thereof a therapeutic amount of a composition comprising a polypeptide of claim 10 or 20 and a pharmaceutically acceptable carrier.
28. A method of treatment comprising administering to a mammalian subject in need thereof a therapeutic amount of a composition comprising an antibody that specifically binds to a polypeptide of claim 10 or 20 and a pharmaceutically acceptable carrier.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of producing a composite material, the method comprising:
mixing conductive particles and ceramic particles to prepare a mixture; and
jetting an aerosol containing the mixture onto a substrate to form the composite material.
2. A method of producing a piezoelectric actuator, the method comprising:
a first electrode layer forming step for forming a first electrode layer on a substrate by jetting an aerosol containing conductive particles and ceramic particles onto the substrate so as to adhere the conductive particles and the ceramic particles to the substrate;
a piezoelectric layer forming step for forming a piezoelectric layer on the first electrode layer by jetting an aerosol containing piezoelectric particles onto the first electrode layer so as to adhere the piezoelectric particles to the first electrode layer;
an annealing treatment step for performing an annealing treatment for the piezoelectric layer; and
a second electrode layer forming step for forming, on the piezoelectric layer, a second electrode layer paring with the first electrode layer.
3. The method according to claim 2, wherein the ceramic particles are particles of one of alumina and zirconia; and the conductive particles are particles of one of Ag and Au.
4. The method according to claim 2, further comprising, before the first electrode layer forming step, forming an diffusion-preventive layer which prevents an element contained in the substrate from diffusing into the piezoelectric layer by jetting onto the substrate an aerosol containing ceramic particles to adhere the ceramic particles to the substrate.
5. The method according to claim 4, wherein the diffusion-preventive layer is formed only at a portion at which the first electrode layer is to be formed.
6. The method according to claim 2, further comprising, after the first electrode layer forming step, forming a diffusion preventive layer which prevents an element contained in the first electrode layer from diffusing into the piezoelectric layer by jetting onto the first electrode layer an aerosol containing ceramic particles to adhere the ceramic particles to the first electrode layer.
7. The method according to claim 2, wherein a mixing ratio of the conductive particles is 4 to 50 weight % in the first electrode layer forming step.
8. A method of producing a piezoelectric actuator, comprising:
forming a multilayer film by jetting an aerosol with an aerosol deposition method while changing one of a composition and a component of the aerosol;
performing an annealing treatment for the multilayer film; and
forming an electrode on the multilayer film.
9. The method according to claim 8, wherein the step of forming the multilayer film includes:
forming a first electrode layer by jetting conductive particles and ceramic particles at a predetermined ratio;
forming an intermediate layer by jetting onto the first electrode layer an aerosol containing only ceramic particles; and
forming a second electrode layer by jetting onto the intermediate layer conductive particles and ceramic particles at a predetermined ratio.
10. The method according to claim 9, wherein the ceramic particles for forming the intermediate layer are piezoelectric ceramic particles.
11. The method according to claim 9, wherein the ceramic particles for forming the second electrode layer are particles of one of alumina and zirconia; and
the conductive particles for forming the second electrode layer are particles of one of Ag and Au.
12. A method of producing an ink-jet head constructed by joining a piezoelectric actuator produced by the producing method as defined in claim 4 and an ink-channel forming body provided with ink channels, the method comprising:
providing a metallic substrate as the substrate;
a step for forming the ink-channel forming body by forming holes which are to be the ink channels in a plurality of metallic plates formed of a same material as that of the metallic substrate, and by stacking and joining the metallic plates together; and
a joining step for joining the metallic substrate to an upper surface of the ink-channel forming body.
13. A method of producing an ink-jet head constructed by joining a piezoelectric actuator, produced by the producing method as defined in claim 7, and an ink-channel forming body provided with ink channels, the method comprising:
providing a metallic substrate as the substrate;
a step for forming the ink-channel forming body by forming holes which are to be the ink channels in a plurality of metallic plates formed of a same material as that of the metallic substrate and by stacking and joining the metallic plates together; and
a joining step for joining the metallic substrate to an upper surface of the ink-channel forming body.
14. A piezoelectric actuator comprising:
a substrate;
a first electrode layer formed on the substrate;
a piezoelectric layer formed on the first electrode layer; and
a second electrode layer formed on the piezoelectric layer;
wherein a conductive material is dispersed in ceramic particles in the first electrode layer.
15. The piezoelectric actuator according to claim 14, wherein the ceramic particles are particles of one of alumina and zirconia; and the conductive particles are particles of one of Ag and Au.