1. A feeding mechanism adapted for use with a woodworking machine, the woodworking machine including a worktable that is disposed for supporting a workpiece thereon and that has a workpiece feeding side and a workpiece discharge side opposite to the workpiece feeding side, and a cutter that is provided on the worktable between the workpiece feeding side and the workpiece discharge side, said feeding mechanism comprising:
a rail unit adapted to be disposed on the workpiece feeding side and the workpiece discharge side of the worktable and extending along a first direction;
a feeding unit including
a slidable body that is adapted to extend across the worktable in a second direction transverse to the first direction, that is disposed slidably on said rail unit, and that has an abutment surface adapted to be perpendicular to the worktable and extending in the second direction,
two rollers that are disposed on said slidable body and that are spaced apart from each other in the second direction,
a belt component that is trained on said rollers and that abuts against said abutment surface of said slidable body, and
a driving component that is disposed for driving said rollers to rotate along with said belt component; and
a press roller unit adapted to extend slidably across the worktable in the second direction and including a slidable seat that is disposed slidably on said rail unit, a press roller that is connected pivotally to said slidable seat, and a resilient component that is adapted for biasing said press roller to press the workpiece against said feeding unit.
2. The feeding mechanism as claimed in claim 1, wherein said slidable body of said feeding unit includes an abutment plate on which said abutment surface is formed, a first positioning plate mounted securely on one end of said abutment plate adjacent to the workpiece discharge side of the worktable of the woodworking machine, and a second positioning plate connected movably to the other end of said abutment plate adjacent to the workpiece feeding side of the worktable, said first and second rollers of said feeding units being disposed respectively on said first and second positioning plates.
3. The feeding mechanism as claimed in claim 2, wherein:
said slidable body of said feeding unit further includes a third positioning plate spaced apart from said second positioning plate and mounted securely on the end of said abutment plate adjacent to the workpiece feeding side of the worktable of the woodworking machine; and
said feeding unit further includes a distance-adjusting unit operable for driving movement of said second positioning plate relative to said third positioning plate in the second direction, said distance-adjusting unit including
two pairs of first lugs provided on said third positioning plate,
two pairs of second lugs provided on said second positioning plate, each pair of said second lugs being registered with a respective pair of said first lugs in the second direction,
a pair of guide rods disposed securely on said third positioning plate, each of said guide rods extending through a respective pair of said first lugs and a corresponding pair of said second lugs,
a pair of springs sleeved respectively on said guide rods, each of said springs being disposed between a respective pair of said first lugs and a corresponding pair of said second lugs,
a handle rod having an end connected pivotally to said third positioning plate, and
a connecting rod having two ends connected respectively and pivotally to an intermediate portion of said handle rod and said second positioning plate.
4. The feeding mechanism as claimed in claim 2, wherein:
said second roller of said feeding unit has an axle extending through said second positioning plate; and
said feeding unit further includes an angle-adjusting unit having a pivot shaft that is provided on said second positioning plate and that extends through said axle of said second roller in the first direction such that said axle is pivotable about said pivot shaft, a lug plate that is disposed securely on said second positioning plate, and an adjusting screw rod extending through and co-movable with an end of said axle of said second roller at a position above said pivot shaft and connected to said lug plate such that movement of said adjusting screw rod relative to said lug plate results in pivoting movement of said axle of said second roller about said pivot shaft.
5. The feeding mechanism as claimed in claim 1, wherein:
said rail unit includes a pair of parallel main rails extending in the first direction and adapted to be disposed respectively on the workpiece feeding side and the workpiece discharge side of the worktable of the woodworking machine, and a pair of secondary rails parallel to said main rails and connected securely and respectively to opposite ends of said slidable body of said feeding unit;
said slidably body is disposed slidably on said main rails; and
said slidable seat of said press roller unit includes a pair of sleeves sleeved respectively and movably on said secondary rails, and a supporting arm interconnecting fixedly said sleeves and extending across the worktable.
6. The feeding mechanism as claimed in claim 5, wherein said press roller unit further includes a pivot rod extending in a third direction transverse to the first and second directions and connected to said supporting arm of said slidable seat, and a connecting plate having an end connected to and pivotable about said pivot rod and an opposite end connected pivotally to said press roller, said resilient component being disposed between said connecting plate and said supporting arm.
7. The feeding mechanism as claimed in claim 6, wherein said connecting plate of said press roller unit further has a stop portion abutting against said supporting arm of said slidable seat of said press roller unit.
8. The feeding mechanism as claimed in claim 6, wherein said press roller unit further includes a U-shaped fastening member sleeved fixedly on said supporting arm of said slidable seat and having two ends defining an opening through which said pivot rod extends.
9. The feeding mechanism as claimed in claim 1, wherein:
said rail unit includes a pair of parallel main rails extending in the first direction and adapted to be disposed respectively on the workpiece feeding side and the workpiece discharge side of the worktable of the woodworking machine;
said slidably body is disposed slidably on said main rails; and
said slidable seat of said press roller unit includes a pair of sleeves sleeved respectively and movably on said main rails, and a supporting arm interconnecting fixedly said sleeves and extending across the worktable.
10. The feeding mechanism as claimed in claim 9, wherein said press roller unit further includes a pivot rod extending in a third direction transverse to the first and second directions and connected to said supporting arm of said slidable seat, and a connecting plate having an end connected to and pivotable about said pivot rod and an opposite end connected pivotally to said press roller, said resilient component being disposed between said connecting plate and said supporting arm.
11. The feeding mechanism as claimed in claim 10, wherein said connecting plate of said press roller unit has a stop portion abutting against said supporting arm of said slidable seat of said press roller unit.
12. The feeding mechanism as claimed in claim 10, wherein said press roller unit further includes a U-shaped fastening member sleeved fixedly on said supporting arm of said slidable seat and having two ends defining an opening through which said pivot rod extends.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An isolated peptide having cytotoxic T cell inducibility, wherein said peptide derived from amino acid sequence of SEQ ID NO: 2, 4, or 6.
2. An isolated peptide of less than about 15 amino acids selected from the group consisting of peptides comprising the amino acid sequences of SEQ ID NO: 7, 8 and 12, or a peptide having cytotoxic T cell inducibility, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 7, 8 and 12, wherein 1, 2, or several amino acids are substituted, deleted, or added.
3. The peptide having cytotoxic T cell inducibility of claim 2, wherein the second amino acid from the N-terminus is phenylalanine, tyrosine, methionine, or tryptophan.
4. The peptide having cytotoxic T cell inducibility of claim 2, wherein the C-terminal amino acid is phenylalanine, leucine, isoleucine, tryptophan, or methionine.
5. An isolated peptide of less than about 15 amino acids selected from the group consisting of peptides comprising the amino acid sequences of SEQ ID NO: 9, 10, 11, 192, 195, 197, 209, 225, 226, 228, 230, 240, 241, 243, 244, 253, 254 and 255, or a peptide having cytotoxic T cell inducibility, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 9, 10, 11, 192, 195, 197, 209, 225, 226, 228, 230, 240, 241, 243, 244, 253, 254 and 255, wherein 1, 2, or several amino acids are substituted, deleted, or added.
6. The peptide having cytotoxic T cell inducibility of claim 5, wherein the second amino acid from the N-terminus is leucine or methionine.
7. The peptide having cytotoxic T cell inducibility of claim 5, wherein the C-terminal amino acid is valine or leucine.
8. A vector in which the DNA encodes peptides of any one of claims 1 to 7.
9. A pharmaceutical composition for treating or preventing a disease associated with over-expression of the genes of SEQ ID NO: 1, 3 andor 5, said composition comprising one or more peptides of claim 2 or 5.
10. The pharmaceutical composition of claim 9, wherein the disease is cancer.
11. The pharmaceutical composition of claim 10, wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, gastric cancer, NSCLC, lymphoma, osteosarcoma, prostate cancer, renal carcinoma, SCLC and soft tissue tumor.
12. An exosome that presents on its surface a complex comprising a peptide of claim 2 or 5 and an HLA antigen.
13. The exosome of claim 12, wherein the HLA antigen is HLA-A24.
14. The exosome of claim 13, wherein the HLA antigen is HLA-A2402.
15. The exosome of claim 12, wherein the HLA antigen is HLA-A2.
16. The exosome of claim 13, wherein the HLA antigen is HLA-A0201.
17. A method of inducing antigen-presenting cells having a high cytotoxic T cell inducibility comprising the step of contacting an antigen-presenting cell with a peptide of claim 2 or 5.
18. A method of inducing cytotoxic T cells by contacting a T cell with a peptide of claim 2 or 5.
19. A method of inducing antigen-presenting cells having high cytotoxic T cell inducibility, said method comprising the step of transferring a gene comprising a polynucleotide encoding a peptide of claim 2 or 5 to an antigen-presenting cell.
20. An isolated cytotoxic T cell, which is induced by contacting a T cell with a peptide of any one of claims 1 to 8 or which is transduced with the nucleic acids encoding the TCR subunits polypeptides binding with a peptide of any one claims 1 to 8 in the context of HLA-A24 or HLA-A2.
21. An antigen-presenting cell, which comprises a complex formed between an HLA antigen and a peptide of claim 2 or 5.
22. The antigen-presenting cell of claim 21, induced by the method of claim 17.
23. A vaccine for inhibiting proliferation of cells expressing genes of SEQ ID NO: 1, 3 andor 5, wherein the vaccine comprises a peptide of claim 2 or 5 as the active ingredient.
24. The vaccine of claim 23, wherein the cell is a cancer cell.
25. The vaccine of claim 24, wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, gastric cancer, NSCLC, lymphoma, osteosarcoma, prostate cancer, renal carcinoma, SCLC and soft tissue tumor.
26. The vaccine of claim 23, formulated for administration to a subject whose HLA antigen is HLA-A24 or HLA-A2.
27. A method of treating or preventing a disease associated with the over-expression of the genes of SEQ ID NO: 1, 3 andor 5 in a subject comprising administering to said subject a vaccine comprising one or more peptide of claim 2 or 5, an immunologically active fragment thereof, or a polynucleotide encoding said peptide or immunologically active fragment.
28. The method of claim 27, wherein the disease is cancer.
29. The method of claim 28, wherein the cancer is selected from the group consisting of bladder cancer, breast cancer, cervical cancer, cholangincellular carcinoma, CML, colorectal cancer, gastric cancer, NSCLC, lymphoma, osteosarcoma, prostate cancer, renal carcinoma, SCLC and soft tissue tumor.