1. A system for searching data in a distributed computing network of sellers’ commercial databases, the system comprising:
a distributed computing network comprising a plurality of sellers’ commercial databases, said seller’s commercial databases commonly related to an industry, at least one buyer’s node adapted to translate a buyer’s first query, a commercial search agent adapted to receive the buyer’s first query regarding a selected item, said item one of a product or service, said commercial search agent in communication with said plurality of sellers’ commercial databases, and said commercial search agent further adapted to search said plurality of sellers’ commercial databases for information related to said selected item; and
at least one intelligent negotiation agent in communication with said commercial agent, said intelligent negotiation agent for receiving configured to receive said information related to said selected item and for engaging to engage in a negotiation for procurement of said selected item.
2. The system for searching data in a distributed computing network of claim 1, wherein:
said commercial search agent comprises an intelligent agent.
3. The system for searching data in a distributed computing network of claim 1, further comprising:
at least one of a plurality of analytical agents for mining data related to said selected item from said plurality of sellers’ databases, said analytical agent also for generating a report on said data that most closely meets a goal, said analytical agent in communication with said commercial search agent for transmission thereto of said report.
4. The system for searching data in a distributed computing network of claim 1, further comprising:
at least one of said plurality of sellers’ databases includes at least one promotion module.
5. The system for searching data in a distributed computing network of claim 1, further comprising:
at least one of said plurality of sellers’ databases includes at least one risk management option module.
6. The system for searching data of claim 1, wherein:
said distributed computing network comprises a cooperative communications network having a plurality of sellers’ commercial databases, said cooperative communications network related to said selected item.
7. A method for searching data in a distributed computing network of sellers’ commercial databases, the method comprising:
receiving a buyer’s first query regarding a selected item, said item one of a product or service, performing an automated search on a distributed computing network for information related to said selected item, said distributed computing network comprising a plurality of sellers’ commercial databases, said plurality of sellers’ commercial databases commonly related to an industry; and receiving from at least one of said plurality of sellers’ commercial databases a risk management option comprising at least one contract contingency authorizing a seller to pay a buyer a penalty if said seller elects in response to an election by the seller to sell said selected item to another buyer.
8. The method for searching data in a distributed computing network of claim 7, wherein:
said performing an automated search is accomplished by invoking an intelligent commercial search agent.
9. The method for searching data in a distributed computing network of claim 7, further comprising:
mining data from said plurality of sellers’ commercial databases, receiving a goal, analyzing said data for generating a report thereon that most closely satisfies said goal, transmitting said report to said commercial search agent.
10. The method for searching data in a distributed computing network of claim 7, further comprising:
receiving from at least one of said plurality of sellers’ commercial databases a promotion.
11. A system for procurement that communicates over a distributed network, the system comprising:
one or more memories for storing a list of individual product items and individual service items, an intelligent commercial search agent in communication with said one or more memories, and one or more seller showcase databases in communication with said commercial search agent, wherein, when one or more of said seller showcase databases receives from said commercial search agent a request to receive bids to sell a selected item specified from said list, said one or more seller showcase databases submits that bid to said commercial search’ agent; and an intelligent analytical agent for mining configured to mine data related to a selected item from said list, said mining from at least one of a plurality of market databases, said analytical agent for generating configured to generate a subset of data that most closely conforms to a set of preprogrammed item parameters, and said analytical agent in communication with said commercial search agent for providing and configured to provide thereto said subset of data.
12. The system of claim 11, further comprising:
said request for bids including a set of item parameters, said set including quality, and receiving from at least one of a plurality of seller’s intelligent agents registered with a cooperative communications network an automated bid to sell said selected item, said bid responsive to said request.
13. The system of claims 11, further comprising:
said request for bids including a set of item parameters, said set including price.
14. The system of claim 11, further comprising:
said request for bids including a set of item parameters, said set including item features.
15. The system of claim 11, further comprising:
said buyer’s intelligent agent comprises an intelligent commercial search agent for searching said plurality of seller’s intelligent agents for bids conforming to a set of preprogrammed item parameters.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1) A method of producing a cloned non-human mammalian NT embryo, the method comprising introducing metaphase donor genetic material from a species into an oocyte of the same species to yield a cloned non-human mammalian NT embryo.
2) The method of claim 1 wherein the donor genetic material introduced into the oocyte comprises a nucleus.
3) The method of claim 1 wherein the donor genetic material introduced into the oocyte comprises an isolated nucleus.
4) The method of claim 1 wherein the donor genetic material introduced into the oocyte is present in a donor cell.
5) The method of claim 4 further comprising fusing the donor cell and the oocyte.
6) The method of claim 1 wherein the donor genetic material introduced into the oocyte is obtained from a differentiated cell.
7) The method of claim 6 wherein the differentiated cell is selected from the group consisting of a fibroblast, an epithelial cell, a hematopoietic cell, and a lymphocyte.
8) The method of claim 7 wherein the epithelial cell is a cumulus cell.
9) The method of claim 6 wherein the differentiated cell is obtained from a source selected from the group consisting of a late embryogenic stage embryo, a fetus, an adult, and a cultured cell line.
10) The method of claim 1 wherein the donor genetic material comprises transgenic DNA.
11) The method of claim 1 further comprising activating the oocyte or the NT embryo.
12) The method of claim 11 wherein activating the oocyte occurs before the donor genetic material is introduced into the oocyte.
13) The method of claim 11 wherein activating the oocyte or the NT embryo occurs at about the same time the donor genetic material is introduced into the oocyte.
14) The method of claim 11 wherein activating the NT embryo occurs after the donor genetic material is introduced into the oocyte.
15) The method of claim 11 wherein activating comprises introducing to the oocyte or the NT embryo cytoplasm from a fertilized oocyte.
16) The method of claim 11 wherein activating comprises removing the donor genetic material from the NT embryo and introducing the donor genetic material to an enucleated fertilized oocyte.
17) The method of claim 11 wherein activating comprises artificially activating the oocyte or the NT embryo.
18) The method of claim 11 wherein activating comprises contacting the oocyte or NT embryo with cycloheximide.
19) The method of claim 1 further comprising enucleating the oocyte before introducing the donor genetic material.
20) The method of claim 1 further comprising enucleating the NT embryo after introducing the donor genetic material to the oocyte, wherein enucleating the NT embryo comprises removal of maternal genetic material.
21) The method of claim 1 wherein the oocyte is arrested at metaphase I as a result of exposure to an arresting agent.
22) The method of claim 21 wherein the oocyte is enucleated while in metaphase I.
23) The method of claim 1 wherein the non-human mammal is a pig.
24) The method of claim 1 wherein the non-human mammal is a cow.
25) The method of claim 1 further comprising incubating the NT embryo such that the NT embryo undergoes cell division.
26) A method of producing a cloned non-human mammal, the method comprising introducing donor genetic material of a species into an oocyte of the same species to yield a cloned non-human mammalian NT embryo and incubating the NT embryo such that the NT embryo undergoes cell division wherein:
a) the donor genetic material is at metaphase; and
b) incubating the NT embryo occurs after transfer of the NT embryo to a host mammal.
27) A method of producing a cloned non-human mammal, the method comprising introducing donor genetic material of a species into an oocyte of the same species to yield a cloned non-human mammalian NT embryo and incubating the NT embryo such that the NT embryo undergoes cell division wherein:
a) the donor genetic material is at metaphase, and
b) incubating the NT embryo comprises culturing the NT embryo in vitro until at least the 2-cell stage.
28) The method of claim 27 further comprising transferring the NT embryo to a host mammal of the same species after the in vitro incubation.
29) The method of claim 28 wherein the NT embryo undergoes cell division in the host mammal and develops into a fetus.
30) The method of claim 28 wherein the NT embryo undergoes cell division in the host mammal and develops into an offspring.