1. A polynucleotide comprising a nucleic acid sequence coding for a fusion protein, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal,
wherein the antibody fragment is a scFv encoded by Seq. ID. No. 1.
2. The polynucleotide of claim 1, further comprising a regulatory sequence for expression in a plant, plant organ, plant tissue, or plant cell.
3. A vector comprising the polynucleotide of claim 1.
4. An expression cassette comprising the polynucleotide of claim 1.
5. The polynucleotide according to claim 2, wherein the regulatory sequence is selected from the group consisting of constitutive, chimeric, tissue specific, inducible synthetic, and cryptic promoters.
6. The polynucleotide according to claim 1, wherein the AFP, and the antibody fragment are arranged in any order relative to each other.
7. A composition comprising a purified preparation of a polynucleotide comprising a nucleic acid sequence coding for a fusion protein, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the antibody fragment is a scFv encoded by Seq. ID. No. 1.
8. A method for the production of Ascomyceta-resistant transgenic plants, plant cells, or plant tissues, the method comprising the introduction of a fusion protein into a genome of a plant, plant cell, or plant tissue, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the antibody fragment is a scFv encoded by Seq. ID. No. 1.
9. A kit comprising a fusion protein, a polynucleotide coding for the fusion protein, or a combination thereof, with the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the antibody fragment is a scFv encoded by Seq. ID. No. 1.
10. The kit according to claim 9, wherein the antibody fragment comprises an enzyme for ELISA or immunoblot detection.
11. The kit according to claim 10, wherein the enzyme is an alkaline phosphatase or a horse radish peroxidase.
12. A polynucleotide comprising a nucleic acid sequence coding for a fusion protein, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal,
wherein the AFP is encoded by Seq. ID. No. 12.
13. The polynucleotide of claim 12, further comprising a regulatory sequence for expression in a plant, plant organ, plant tissue, or plant cell.
14. The polynucleotide according to claim 13, wherein the regulatory sequence is selected from the group consisting of constitutive, chimeric, tissue specific, inducible synthetic, and cryptic promoters.
15. A vector comprising the polynucleotide of claim 12.
16. An expression cassette comprising the polynucleotide of claim 12.
17. The polynucleotide of claim 12, wherein a scFv encoding sequence is linked to an AFP encoding sequence via Seq. ID. No. 19, and
the scFv encoding sequence has the 5\u2032 position with the AFP encoding portion in the 3\u2032 position, or
the AFP encoding portion has the 5\u2032 position and the and scFv encoding sequence has the 3\u2032 position.
18. The polynucleotide according to claim 12, wherein the AFP, and the antibody fragment are arranged in any order relative to each other.
19. A composition comprising a purified preparation of a polynucleotide comprising a nucleic acid sequence coding for a fusion protein, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the AFP is encoded by Seq. ID. No. 12.
20. A method for the production of Ascomyceta-resistant transgenic plants, plant cells, or plant tissues, the method comprising the introduction of a fusion protein into a genome of a plant, plant cell, or plant tissue, the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the AFP is encoded by Seq. ID. No. 12.
21. A kit comprising a fusion protein, a polynucleotide coding for the fusion protein, or a combination thereof, with the fusion protein comprising
a) an anti-fungal protein or peptide (AFP) and an antibody fragment, specifically recognising an epitope of an Ascomyceta, and
b) a cellular targeting signal
wherein the AFP is encoded by Seq. ID. No. 12.
22. The kit according to claim 21, wherein the antibody fragment comprises an enzyme for ELISA or immunoblot detection.
23. The kit according to claim 22, wherein the enzyme is an alkaline phosphatase or a horse radish peroxidase.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A coating composition comprising a hardenable film-former and an effective amount of a reducing agent having a redox potential sufficient to decolorize an iodine or iodophor stain on the film-former, wherein the reducing agent comprises a sulfite, hydrosulfite, nitrite, hydronitrite or hydrophospite and will at least partially decolorize a stain caused by the iodine complexed with polyvinylpyrrolidone when applied to the stain, allowed to stand for one hour at room temperature, and evaluated by visual observation of the stain under average overhead fluorescent illumination.
2. A composition according to claim 1 wherein the reducing agent will at least partially decolorize a plurality of stain applications on the same region of the surface.
3. A composition according to claim 1 wherein the reducing agent has a redox potential from +0.558 to \u22124.00 volts at 25\xb0 C.
4. A composition according to claim 1 wherein the reducing agent comprises a sulfite.
5. A composition according to claim 1 comprising about 1 to about 10 weight percent reducing agent.
6. A composition according to claim 1 comprising about 1 to about 4 weight percent reducing agent.
7. A coating composition according to claim 1 wherein the film-former forms a hardened film resistant to rinsing with tap water.