1. A high pressure polymerization process to form an ethylene-based polymer, the process comprising at least the following steps:
feeding ethylene to a first reaction zone and to one or more subsequent reaction zones, and wherein for each subsequent reaction zone that receives fresh ethylene, the ratio, Rn (n=reaction zone number, n>1), of \u201cmass fraction of fresh ethylene fed to the first reaction zone (RZ1)\u201d to \u201cmass fraction of fresh ethylene fed to the nth reaction zone (RZn)\u201d is (Rn=RZ1RZn) greater than 1, or is from 0 to 0.25, and
wherein the \u201ctotal amount of ethylene fed to the polymerization process\u201d derives from at least one fresh ethylene stream and at least one recycled ethylene stream, and wherein the at least one recycled ethylene stream comprises at least one chain transfer agent andor comprises at greater than, or equal to, 1 weight %, based on total amount of components in recycled ethylene stream, of one or more non-ethylene components, including the CTA, and
wherein the inlet stream to each reaction zone comprises less than, or equal to, 5 weight ppm oxygen, based on the total weight of mass flows fed to the reaction zone.
2. The process of claim 1, wherein the process comprises at least one Primary compressor and at least one Booster compressor.
3. The process of claim 2, wherein the ethylene stream from the Booster compressor is fed only to one Primary compression flow, and wherein the Primary compressor has at least two separate compression flows.
4. The process of claim 1, wherein Rn is greater than 1.
5. The process of claim 1, wherein Rn is zero.
6. The process of claim 1, wherein the first reaction zone is a tubular reaction zone.
7. The process of claim 1, wherein, when no (0%) fresh ethylene is fed to the first reaction zone, the \u201camount of ethylene fed to the first reaction zone\u201d derives only from at least one recycled ethylene stream.
8. The process of claim 1, wherein the \u201ctotal amount of ethylene fed to the polymerization process\u201d derives from a fresh ethylene stream and at least one recycled ethylene stream, and
wherein the at least one recycled ethylene stream comprises at least one chain transfer agent, and
wherein, when no (0%) fresh ethylene is fed to the first reaction zone, than the \u201camount of ethylene fed to the first reaction zone\u201d derives only from the at least one recycled ethylene stream.
9. The process of claim 1, wherein from \u201cgreater than 0\u201d to 100 weight percent of the total amount of fresh ethylene fed to the polymerization process is fed to the first reaction zone andor to a sequential reaction zone.
10. The process of claim 1, wherein the process comprises only one Primary compressor.
11. The process of claim 1, wherein an initiator is added to at least one reaction zone of the polymerization, and wherein the initiator has a \u201chalf-life temperature at one second\u201d greater than 255\xb0 C.
12. A high pressure polymerization process to form an ethylene-based polymer, the process comprising at least the following steps:
feeding ethylene to a first reaction zone and to one or more subsequent reaction zones, and wherein 100 weight percent of the total amount of fresh ethylene fed to the polymerization process is fed to the first reaction zone, and
wherein the \u201ctotal amount of ethylene fed to the polymerization process\u201d derives from at least one fresh ethylene stream and at least one recycled ethylene stream, and wherein the at least one recycled ethylene stream comprises at least one chain transfer agent andor comprises greater than, or equal to, 1 weight %, based on total amount of components in recycled ethylene stream, of one or more non-ethylene components, including the CTA, and
wherein the inlet stream to each reaction zone comprises less than, or equal to, 5 weight ppm oxygen, based on the total weight of mass flows fed to the reaction zone.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of: a base protecting group, a 2\u2032-hydroxyl protecting group, and a combination thereof, and
deprotecting at least one of the protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an \u03b1-effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the \u03b1-effect nucleophile has a pKa of about 4 to 13.
2. The method of claim 1, wherein the \u03b1-effect nucleophile is a peroxyanion.
3. The method of claim 1, wherein the \u03b1-effect nucleophile is selected from at least one of the following: hydrogen peroxide and salts thereof, a peracid and salts thereof, a perboric acid and salts thereof, an alkylperoxide and salts thereof, a hydroperoxide and salts thereof, butylhydroperoxide and salts thereof, benzylhydroperoxide and salts thereof, phenylhydroperoxide and salts thereof, performic acid and salts thereof, peracetic acid and salts thereof, perbenzoic acid and salts thereof, chloroperbenzoic acid and salts thereof, and combinations thereof.
4. The method of claim 1, wherein the \u03b1-effect nucleophile comprises a hydroperoxide, wherein the solution is at a pH of about 7 to 10, and wherein the hydroperoxide has a pKa of about 8 to 13.
5. The method of claim 1, wherein the \u03b1-effect nucleophile comprises hydrogen peroxide, wherein the solution is at a pH of about 8 to 10, and wherein the hydrogen peroxide has a pKa of about 11 to 13.
6. The method of claim 1, wherein the \u03b1-effect nucleophile comprises a peracid, wherein the solution is at a pH of about 7 to 10, and wherein the peracid has a pKa of about 6 to 11.
7. The method of claim 1, wherein the \u03b1-effect nucleophile comprises a perboric acid, wherein the solution is at a pH of about 8 to 10, and wherein the perboric acid has a pKa of about 9 to 12.
8. The method of claim 1, wherein the \u03b1-effect nucleophile comprises an alkylperoxide, wherein the solution is at a pH of about 8 to 10, and wherein the alkylperoxide has a pKa of about 9 to 13.
9. The method of claim 1, wherein the \u03b1-effect nucleophile comprises a hydrogen peroxide salt, wherein the solution is at a pH of about 8 to 10, and wherein the hydrogen peroxide salt has a pKa of about 11 to 12.
10. The method of claim 1, wherein the \u03b1-effect nucleophile comprises hydrogen peroxide and sodium formate, and wherein the solution is at a pH of about 6 to 11.
11. The method of claim 1, wherein the solution is at a pH of about 7 to 10.
12. The method of claim 1, wherein the synthetic precursor of a nucleic acid is attached to a solid support.
13. The method of claim 12, wherein the synthetic precursor of a nucleic acid is attached to an array.
14. The method of claim 1, wherein the nucleic acid is a DNA.
15. The method of claim 1, wherein the nucleic acid is a RNA.
16. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
providing a synthetic precursor of a nucleic acid comprising at least one protecting group selected from the group consisting of: an exocyclic amino protecting group, an imino protecting group, a 2\u2032-hydroxyl protecting group, and combinations thereof; and
deprotecting at least one of the protecting groups of the synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an \u03b1-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the \u03b1-effect nucleophile has a pKa of about 4 to 13.
17. The method of claim 16, wherein said synthetic precursor of a nucleic acid is attached to a solid support.
18. The method of claim 17, wherein the said synthetic precursor of a nucleic acid is attached to an array.
19. The method of claim 16, wherein the \u03b1-effect nucleophile is a peroxyanion.
20. The method of claim 16, wherein the \u03b1-effect nucleophile is selected from at least one of the following: hydrogen peroxide and salts thereof, a peracid and salts thereof, a perboric acid and salts thereof, an alkylperoxide and salts thereof, a hydroperoxide and salts thereof, butylhydroperoxide and salts thereof, benzylhydroperoxide and salts thereof, phenylhydroperoxide and salts thereof, performic acid and salts thereof, peracetic acid and salts thereof, perbenzoic acid and salts thereof, chloroperbenzoic acid and salts thereof, and combinations thereof.
21. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
providing a synthetic precursor of a nucleic acid comprising at least one exocyclic amino protecting group and optionally comprising at least one protecting group selected from the following: an imino protecting group, a 2\u2032-hydroxyl protecting group, and combinations thereof; and
deprotecting at least one of the exocyclic amino protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an \u03b1-effect nucleophile, wherein the solution is at a pH of about 4 to 11, and wherein the \u03b1-effect nucleophile has a pKa of about 4 to 13.
22. The method of claim 21, wherein said synthetic precursor of a nucleic acid is attached to a solid support.
23. The method of claim 22, wherein said synthetic precursor of a nucleic acid is attached to an array.
24. The method of claim 21, wherein the \u03b1-effect nucleophile is a peroxyanion.
25. The method of claim 21, wherein the \u03b1-effect nucleophile is selected from at least one of the following: hydrogen peroxide and salts thereof, a peracid and salts thereof, a perboric acid and salts thereof, an alkylperoxide and salts thereof, a hydroperoxide and salts thereof, butylhydroperoxide and salts thereof, benzylhydroperoxide and salts thereof, phenylhydroperoxide and salts thereof, performic acid and salts thereof, peracetic acid and salts thereof, perbenzoic acid and salts thereof, chloroperbenzoic acid and salts thereof, and combinations thereof.
26. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
providing a synthetic precursor of a nucleic acid comprising at least one 2\u2032-hydroxyl protecting group and optionally comprising at least one protecting group selected from the group consisting of: an exocyclic amino protecting group, an imino protecting group, and combinations thereof; and
deprotecting the 2\u2032-hydroxyl protecting groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an \u03b1-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the \u03b1-effect nucleophile has a pKa of about 4 to 13.
27. The method of claim 26, wherein said synthetic precursor of a nucleic acid is attached to a solid support.
28. The method of claim 27, wherein said synthetic precursor of a nucleic acid is attached to an array.
29. The method of claim 26, wherein the \u03b1-effect nucleophile is a peroxyanion.
30. The method of claim 26, wherein the \u03b1-effect nucleophile is selected from at least one of the following: hydrogen peroxide and salts thereof, a peracid and salts thereof, a perboric acid and salts thereof, an alkylperoxide and salts thereof, a hydroperoxide and salts thereof, butylhydroperoxide and salts thereof, benzylhydroperoxide and salts thereof, phenylhydroperoxide and salts thereof, performic acid and salts thereof, peracetic acid and salts thereof, perbenzoic acid and salts thereof, chloroperbenzoic acid and salts thereof, and combinations thereof.
31. A method of performing post-synthesis deprotection of a synthetic precursor of a nucleic acid, comprising:
providing a synthetic precursor of a nucleic acid comprising at least one exocyclic amino protecting group and at least one 2\u2032-hydroxyl group, and optionally comprising at least one imino protecting group; and
deprotecting the exocyclic amino protecting groups and the 2\u2032-hydroxyl groups of said synthetic precursor of a nucleic acid by contacting said precursor with a solution comprising an \u03b1-effect nucleophile, wherein the solution is at a pH of about 4 to 10, and wherein the \u03b1-effect nucleophile has a pKa of about 4 to 13.
32. The method of claim 31, wherein said synthetic precursor of a nucleic acid is attached to a solid support.
33. The method of claim 32, wherein said synthetic precursor of a nucleic acid is attached to an array.
34. The method of claim 31, wherein the \u03b1-effect nucleophile is a peroxyanion.
35. The method of claim 31, wherein the \u03b1-effect nucleophile is selected from hydrogen peroxide and salts thereof, a peracid and salts thereof, a perboric acid and salts thereof, an alkylperoxide and salts thereof, a hydroperoxide and salts thereof, butylhydroperoxide and salts thereof, benzylhydroperoxide and salts thereof, phenylhydroperoxide and salts thereof, performic acid and salts thereof, peracetic acid and salts thereof, perbenzoic acid and salts thereof, chloroperbenzoic acid and salts thereof, and combinations thereof.