1. A method for radio frequency resource allocation, the method comprising the acts of:
determining that radio frequency resources are to be allocated to a plurality of mobile stations in an uplink subframe;
determining a signal quality measure for each mobile station of the plurality of mobile stations; and
allocating the radio frequency resources in a time alignment within the uplink subframe for mobile stations of the plurality of mobile stations with a determined signal quality measure below a predetermined threshold and then allocating the radio frequency resources within the uplink subframe for mobile stations of the plurality of mobile stations with a determined signal quality measure above the predetermined threshold, wherein at least a first one of the mobile stations with a determined signal quality measure above the predetermined threshold is allocated the radio frequency resources in time alignment in the uplink subframe and at least a second one of the mobile stations with a determined signal quality measure above the predetermined threshold is allocated the radio frequency resources in frequency alignment in the uplink subframe,
wherein the radio frequency resources includes a number of frequencies and symbols of the subframe, wherein the plurality of mobile stations communicate with a wireless communication system using orthogonal frequency division multiple access (OFDMA),
wherein the mobile stations allocated radio frequency resources in time alignment are allocated more OFDM symbols but less frequencies than the mobile stations allocated radio frequency resources in frequency alignment.
2. The method of claim 1, wherein the radio frequency resource allocation is also based on a quality of service for the plurality of mobile stations.
3. The method of claim 1, further comprising the acts of:
informing the plurality of mobile stations of the radio frequency resource allocation.
4. The method of claim 1, wherein the signal quality measure is a signal-to-interference-plus noise ratio (SINR).
5. A method for radio frequency resource allocation, the method comprising the acts of:
determining that a radio frequency resource is to be allocated to a mobile station;
determining a signal quality measure for the mobile station; and
allocating the radio frequency resource to the mobile station in a time or frequency alignment within an uplink subframe based on the signal quality measure, wherein the radio frequency resource is allocated in a time alignment when the signal quality measure is below a predetermined threshold and the radio frequency resource is allocated in frequency alignment when the signal quality measure is above the predetermined threshold, and wherein the mobile station communicates with a base station using orthogonal frequency division multiple access (OFDMA),
wherein allocation of radio frequency resource in time alignment involves allocating more OFDM symbols but less frequencies than allocation of radio frequency resource in frequency alignment,
wherein radio frequency resources are first allocated to mobile stations with signal quality measures below the predetermined threshold, and then allocated to mobile stations with signal quality measures above the predetermined threshold.
6. The method of claim 5 wherein the predetermined threshold is fixed.
7. The method of claim 5, wherein the predetermined threshold is dynamic.
8. A base station, comprising:
a memory; and
a processor, coupled to the memory, the processor including
a scheduler,
logic for determining a signal quality measure for each of a plurality of mobile stations, and
logic for allocating radio frequency resources in a time alignment within an uplink subframe for mobile stations of the plurality of mobile stations with a determined signal quality measure below a predetermined threshold and then allocating the radio frequency resources within the uplink subframe for mobile stations of the plurality of mobile stations with a determined signal quality measure above the predetermined threshold, wherein at least a first one of the mobile stations with a determined signal quality measure above the predetermined threshold is allocated the radio frequency resources in time alignment in the uplink subframe and at least a second one of the mobile stations with a determined signal quality measure above the predetermined threshold is allocated the radio frequency resources in frequency alignment in the uplink subframe, wherein the radio frequency resource includes a number of frequencies and symbols of the subframe, wherein the plurality of mobile stations communicate with a wireless communication system using orthogonal frequency division multiple access (OFDMA), wherein the mobile stations allocated radio frequency resources in time alignment are allocated more OFDM symbols but less frequencies than the mobile stations allocated radio frequency resources in frequency alignment.
9. The base station of claim 8, wherein the radio frequency resource allocation is also based on a quality of service for the plurality of mobile stations.
10. The base station of claim 8, wherein the signal quality measure is a signal-to-interference-plus noise ratio (SINR).
11. The base station of claim 8, wherein the predetermined threshold is fixed.
12. The base station of claim 8, wherein the predetermined threshold is dynamic.
13. The method of claim 5, wherein the radio frequency resource is allocated to a first plurality of mobile stations in the uplink subframe in time alignment and to a second plurality of mobile stations in the uplink subframe in frequency alignment.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for detecting species in the genus Glycine in cases where even one kind of the genus Glycine is contained in a subject to which the method is to be applied such as a food ingredient or product, which comprises the steps of:
a) collecting 45S rRNA precursor gene sequences containing a ITS sequence of the genus Glycine and plants thought to be related species thereof and then selected the region common to the genus Glycine in the 45S rRNA precursor gene sequences;
b) selecting the base specific to the genus Glycine from the region;
c) designing primers (A) andor (B) having the specific base at 3 end thereof which primers can hybridize under the stringent conditions to a nucleic acid molecule having a common nucleotide base sequence for all species in the genus Glycine in 45S rRNA precursor gene sequence thereof wherein 3 end of primer (A) can complementarily bind to a base in ITS-1 sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule while 3\u2032 end of primer (B) can complementarily bind to a base in ITS-2 sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule;
d) isolating DNA from the subject to which the method is to be applied;
e) conducting PCR amplification for the isolated DNA using at least one member selected from the group consisting of the primers (A) and (B); and
f) identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the genus Glycine.
2. The method of claim 1, wherein the nucleic acid molecule having a common nucleotide sequence for all species in the genus Glycine in 45S rRNA precursor gene sequence thereof is a nucleic acid molecule having a common specific nucleotide sequence for all species in the genus Glycine in l-ITS-1 sequence thereof.
3. The method of claim 1, wherein the nucleic acid molecule having a common nucleotide sequence for all species in the genus Glycine in 45S rRNA precursor gene sequence thereof is a nucleic acid molecule having a common specific nucleotide sequence for all species in the genus Glycine in ITS-2 sequence thereof.
4. The method of claim 2, wherein the step of conducting PCR comprises using the primers (A) and (C), which primer (C) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence in which ITS-1, 5.8S rRNA gene, ITS-2 and LSU rRNA gene sequences of the genus Glycine are continuously bonded.
5. The method of claim 3, wherein the step of conducting PCR comprises using the primers (B) and (D), which primer (D) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence in which SSU rRNA gene, ITS-1, 5.8S rRNA gene and ITS-2 sequences of the genus Glycine are continuously bonded.
6. The method of claim 2, wherein the step of conducting PCR comprises using the primers (A) and (E), which primer (E) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence in which SSU rRNA gene and ITS-I sequences of the genus Glycine are continuously bonded.
7. The method of claim 3, wherein the step of conducing PCT comprises using the primers (B) and (F), which primer (F) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence m which ITS-2 and LSU rRNA sequences of the genus Glycine are continuously bonded.
8. The method of claim 3, wherein the common specific nucleotide sequence for all species in the genus Glycine in ITS-2 sequence thereof is selected from the group consisting of SEQ NOs:31, 32 and 33, and complementary nucleotide sequences thereof.
9. The method of claim 1, wherein the primer (B) is selected from the group consisting of SEQ NOs:34, 35, 36, 37, 38, 39, 40 and 41.
10. The method of claim 4, wherein the primer (C) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence in 5.8S rRNA gene sequence of the genus Glycine, and 3\u2032 end of primer (C) can complementarily bind to a base in 5.8S rRNA gene sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule.
11. The method of claim 5, wherein the primer (D) can hybridize under stringent conditions to a nucleic acid molecule having a part of a nucleotide sequence in 5.8S rRNA gene sequence of the genus Glycine, and 3\u2032 end of primer (D) can complementarily bind to a base in 5.8S rRNA gene sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule.
12. The method of claim 10, wherein the part of nucleotide sequence in 5.8S rRNA gene sequence of the genus Glycine is selected from the group consisting of SEQ NO:1 and a complementary nucleotide sequence thereof.
13. The method of claim 11, wherein the part of nucleotide sequence in 5.8S rRNA gene sequence of the genus Glycine is selected from the group consisting of SEQ NO:1 and a complementary nucleotide sequence thereof.
14. The method of claim 4, wherein the primer (C) is selected from the group consisting of SEQ NOs:2, 3 and 4.
15. The method of claim 5, wherein the primer (D) is selected from the group consisting of SEQ NOs:5, 6 and 7.
16. The method of claim 1, wherein the step of conducting PCR comprises using a combination of a primer of SEQ NO:28 and a primer selected from the group consisting of SEQ NOs:35, 36, 37, 38, 39, 40 and 41 as the primer (B).