1. A travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism, the sewing machine including a control shaft, on the control shaft being provided a control element, the travel beginningend adjusting positioning structure comprising:
a fixed holder being provided with a fixing portion on an end thereof and fixed on the sewing machine;
a positioning holder having an end pivoted on a pivoting element together with the fixed holder, the control shaft being rotatably inserted through the pivot element, the positioning holder being further provided with a positioning portion opposite the fixing portion of the fixed holder, the positioning portion being formed with a through hole, the fixed holder having a side for mounting a motor, on a rotating shaft of the motor being mounted a rotating element, the rotating element being engaged with the control element of the control shaft; and
an adjusting apparatus including an adjusting element, a locking element screwed to the adjusting element, and an elastic element, the adjusting element having a head portion and a threaded adjusting shaft extending from the head portion, the locking element being formed with an arc peripheral surface and a threaded hole in the arc peripheral surface, the adjusting shaft of the adjusting element being inserted through the fixing portion of the fixed holder and the through hole of the positioning portion of the positioning folder, the adjusting shaft being screwed into the threaded hole locking element, the arc peripheral surface of the locking element cooperating with the through hole of the positioning portion of the positioning holder, the locking element rotating in an extending direction of the arc peripheral surface, the elastic element being mounted on the adjusting shaft of the adjusting element with both ends of the elastic element pushing against the fixing portion of the fixed holder and the positioning portion of the positioning holder.
2. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the pivot element includes a pivot sleeve, the positioning holder is formed with a pivot hole.
3. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the locking element is coaxially provided on each of two end surfaces thereof with a limiting cylinder which is smaller in diameter than the locking element, the arc peripheral surface of the locking element cooperates with the through hole of the positioning portion of the positioning holder, the two limiting cylinders push against the positioning portion.
4. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the positioning portion of the positioning holder is provided with a limiting portion protruding at each of two opposite sides of the through hole, the locking element is arranged between the two limiting portions.
5. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the head portion of the adjusting element is formed with an arc convex stopping surface on an end thereof which contacts the fixing portion, the fixing portion includes an insertion hole, and the stopping surface cooperates with the insertion hole.
6. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the positioning holder is provided with a through hole, the fixed holder includes a through hole, the rotating shaft of the motor is inserted through the through hole of the positioning holder and the through hole of the fixed holder.
7. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the locking element is in the form of a column, the through hole of the positioning portion of the positioning holder is a square through hole, the locking element cooperates with the square through hole to keep non-rotating relative to each other.
8. The travel beginningend adjusting positioning structure for a sewing machine motor drive mechanism as claimed in claim 1, wherein the head portion of adjusting element is disc-shaped.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of identifying an agent that alters NAD-dependent deacetylation activity of a Sir2 protein, comprising the steps of:
a) combining an acetylated protein, the agent to be tested and the Sir2 protein or a fragment of the Sir2 protein, wherein the fragment of the Sir2 protein comprises an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 27, SEQ ID NO: 28 and SEQ ID NO: 38;
b) measuring NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein with respect to at least one lysine residue in the acetylated protein; and
c) comparing the NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein with respect to at least one lysine residue in the acetylated protein in the presence of the agent with the NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein with respect to at least one lysine residue in the acetylated protein in the absence of the agent,
wherein a difference in the NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein with respect to at least one lysine residue in the acetylated protein in the presence of the agent identifies the agent that alters NAD-dependent deacetylation of the Sir2 protein.
2. The method of claim 1, wherein the acetylated protein is a nuclear protein.
3. The method according to claim 2, wherein the nuclear protein is a histone protein.
4. The method of claim 1, wherein the acetylated protein is a cytoplasmic protein.
5. The method according to claim 1, wherein the Sir2 protein is a Sir2\u03b1 protein.
6. The method of claim 5, wherein the Sir2\u03b1 protein comprises the amino acid sequence of SEQ ID NO: 1.
7. The method of claim 1, wherein the Sir2 protein is an isolated Sir2 protein.
8. The method of claim 7, wherein the isolated Sir2 protein is a recombinant Sir2 protein.
9. The method of claim 1, wherein the Sir2 protein is a human Sir2 protein.
10. The method of claim 9, wherein the human Sir2 protein comprises SEQ ID NO: 38.
11. The method of claim 1, wherein the Sir2 protein is an isolated nuclear protein.
12. The method of claim 1, wherein the difference in NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein is an increase in NAD-dependent deacetylation activity and identifies the agent that increases NAD-dependent deacetylation activity of the Sir2 protein.
13. The method of claim 12, wherein the agent is an agonist of NAD-dependent deacetylation activity of the Sir2 protein.
14. The method of claim 1, wherein the difference in NAD-dependent deacetylation activity of the Sir2 protein or the fragment of the Sir2 protein is a decrease in NAD-dependent deacetylation activity and identifies the agent that decreases NAD-dependent deacetylation activity.
15. The method of claim 14, wherein the agent is an antagonist of NAD-dependent deacetylation activity of the Sir2 protein.
16. The method of claim 1, wherein the fragment of the Sir2 protein comprises the amino acid sequence of SEQ ID NO: 9.
17. The method of claim 1, wherein the fragment of the Sir2 protein comprises the amino acid sequence of SEQ ID NO: 19.
18. The method of claim 1, wherein the fragment of the Sir2 protein comprises the amino acid sequence of SEQ ID NO: 38.