1. A method of using a competitive type assay, comprising:
running an assay;
incorporating F-labeled or Q-labeled aptamers, wherein said aptamers are labeled with said F’s and Q’s located on the interior portion of said aptamer;
adding a volume of unlabeled analyte, wherein said analyte competes to bind with said F-labeled or Q-labeled analytes;
wherein fluorescence light levels change proportionately in response to the amount of said volume of unlabeled analyte; and
wherein said competitive type assay detects molecules selected from the group consisting of: pesticides, OP nerve agents, OP nerve agent breakdown products, acetylcholine (ACh), acyl homoserine lactone (AHL) and other quorum sensing (QS) molecules, natural and synthetic amino acids and their derivatives, histidine, histamine, homocysteine, DOPA, melatonin, nitrotyrosine, short chain proteolysis products, cadaverine, putrescine, polyamines, spermine, spermidine, nitrogen bases of DNA or RNA, nucleosides, nucleotides, nucleotide cyclical isoforms, cAMP, cGMP, cellular metabolites, urea, uric acid, pharmaceuticals, therapeutic drugs, illegal drugs, narcotics, hallucinogens, gamma-hydroxybutyrate (GHB), cellular mediators, cytokines, chemokines, immune modulators, neural modulators, inflammatory modulators, prostaglandins, prostaglandin metabolites, explosives, trinitrotoluene, explosive breakdown products or byproducts, peptides and their derivatives, such as poly-D-glutamic acid (PDGA) and similar bacterial capsule materials, macromolecules, proteins, bacterial surface proteins, glycoproteins, lipids, glycolipids, nucleic acids, polysaccharides, lipopolysaccharides or LPS components, lipoteichoc or teichoic acids, viruses, whole cells, spores or endospores, and subcellular organelles or cellular fractions.
2. The method of claim 1, further comprising:
immobilizing said small molecules on a column, membrane, plastic or glass bead, magnetic bead, quantum dot, or other matrix;
eluting immobilized aptamers from said column, membrane, plastic or glass bead, magnetic bead, or other matrix by use of 0.2-3.0 M sodium acetate at a pH of between 3 and 7.
3. The method of claim 1, wherein said detected molecules are quantified.
4. A method of using a competitive type assay, comprising:
running an assay; and
incorporating an aptamer, wherein said aptamer is selected from the SEQ Aptamers.
5. The method of claim 4, further comprising:
adding a volume of unlabeled analyte, wherein said analyte competes to bind with said F-labeled or Q-labeled analytes; and
wherein fluorescence light levels change proportionately in response to the amount of said volume of unlabeled analyte.
6. A method of using a competitive type assay, comprising:
running an assay;
incorporating an aptamer;
wherein said aptamer has a binding pocket; and
wherein said binding pocket is comprised of 3 to 6 nucleotides.
7. The method of claim 6 wherein said binding pocket is comprised of 3 or more nucleotides of a specific sequence or arrangement to confer the appropriate volume and conformation in 3-dimensional space to enable optimal binding to target molecules.
8. The method of claim 6 wherein said aptamer is selected from the SEQ Aptamers.
9. The method of claim 7 wherein said aptamer is selected from the SEQ Aptamers.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Cleaning apparatus comprising a filter device, which comprises:
a water container;
an inlet channel connected directly with and for supplying water into the water container;
an outlet channel connected directly with and for removing water out of the water container, the filter device arranged in the outlet channel comprising a ceramic filter; and
a heating device, the heating device being arranged in the ceramic filter,
wherein the pore diameter of the ceramic filter is less than 10 micrometers and wherein the heating device is provided for thermal sterilization of the ceramic filter by heating up the ceramic filter to a temperature of at least 500 \xb0 C.
2. Cleaning apparatus according to claim 1, wherein the filter device comprises two ceramic filters arranged one behind the other.
3. Cleaning apparatus according to claim 1, wherein the ceramic filter equipped with the heating device comprises a plurality of ceramic layers which are planar or extend in wave shape.
4. Cleaning apparatus according to claim 1, wherein the heating device is connected with a control unit.
5. Cleaning apparatus according to claim 4, wherein the control unit automatically activates the heating device on actuation of the switching-on andor switching-off button of the cleaning apparatus.
6. Cleaning apparatus according to claim 4, wherein the control unit activates the heating device automatically at predetermined operating time intervals.
7. Cleaning apparatus according to claim 1, wherein it is a multi-function cleaning apparatus operable in different operating modes.
8. Cleaning apparatus according to claim 7, wherein a first operating mode is a vacuum cleaner operation and a second operating mode is an air cleaning operation.
9. Cleaning apparatus according to claim 1, wherein it comprises a pre-filter arranged in the inlet channel.
10. Cleaning apparatus according to claim 9, wherein the pre-filter consists of polymer woven material.
11. Cleaning apparatus according to claim 1, wherein it is an air-conditioning apparatus.
12. Cleaning apparatus comprising a filter device, which comprises a ceramic filter and a heating device arranged in the ceramic filter, wherein the pore diameter of the ceramic filter is less than 10 micrometers, wherein the heating device is provided for thermal sterilization of the ceramic filter by heating up the ceramic filter to a temperature of at least 500\xb0 C., wherein the filter device comprises two ceramic filters arranged one behind the other in an outlet channel, and wherein the first ceramic filter arranged in an outlet channel is an adsorption filter which is provided for filtering out coarser particles and droplets and the second ceramic filter arranged in the outlet channel is the ceramic filter equipped with the heating device.
13. Cleaning apparatus according to claim 12, wherein the first ceramic filter arranged in the outlet channel is a ceramic foam filter.
14. Cleaning apparatus according to claim 13, wherein the first ceramic filter arranged in the outlet channel comprises aluminium oxide as filter material.
15. Cleaning apparatus according to claim 13 wherein the first ceramic filter arranged in the outlet channel comprises a frame consisting of polymer material.
16. Cleaning apparatus comprising a filter device, which comprises a ceramic filter and a heating device, the heating device being arranged in the ceramic filter, wherein the pore diameter of the ceramic filter is less than 10 micrometers and wherein the heating device is provided for thermal sterilization of the ceramic filter by heating up the ceramic filter to a temperature of at least 500\xb0 C, wherein the filter device comprises two ceramic filters one behind the other and wherein the filter device comprises a vacuum pump arranged in the outlet channel between the two ceramic filters.
17. Cleaning apparatus comprising a filter device, which comprises a ceramic filter and a heating device, the heating device being arranged in the ceramic filter, wherein the pore diameter of the ceramic filter is less than 10 micrometers and wherein the heating device is provided for thermal sterilization of the ceramic filter by heating up the ceramic filter to a temperature of at least 500\xb0 C., wherein the filter device comprises two ceramic filters one behind the other and wherein the filter device comprises a vacuum pump arranged in the outlet channel behind the two ceramic filters.
18. Cleaning apparatus comprising a filter device, which comprises a ceramic filter and a heating device, the heating device being arranged in the ceramic filter, wherein the pore diameter of the ceramic filter is less than 10 micrometers and wherein the heating device is provided for thermal sterilization of the ceramic filter by heating up the ceramic filter to a temperature of at least 500\xb0 C., and which comprises a pre-filter arranged in a nozzle mounting region of the cleaning apparatus to be withdrawable.