1461141972-645c3191-5731-4d66-ae9c-8725eb3fa296

What we claim:

1. In a cell culture medium comprising an aqueous solution of amino acids and a carbohydrate energy source for cells cultured in said medium,
the improvement wherein said aqueous solution
(i) is substantially saturated with said amino acids at a temperature of 30 to 50 C.;
(ii) has an osmolarity of 320 to 450 mOsm;
(iii) contains 5.50 to 20 grams per liter of amino acids and 5.50 to 20 grams per liter of said carbohydrate energy source
and wherein the dry weight of the amino acids in solution in said medium comprises at least 20% of the total dry weight of all solid components present in said medium.
2. The claim 1 cell culture medium in which said carbohydrate energy source is glucose.
3. The claim 1 cell culture medium in which said osmolarity of from 320 to 450 mOsm is provided at least in part by sodium salt.
4. The claim 1 or claim 2 cell culture medium in which said osmolarity of from 320 to 450 mOsm is provided at least in part by a sodium chloride osmolyte.
5. The claim 1 cell culture medium further comprising a suspension of undissolved amino acids in said aqueous solution substantially saturated with amino acids
wherein said substantial saturation is maintained as cell growth consumes amino acids in solution in said aqueous medium.
6. In an aqueous basal cell culture medium, the improvement which comprises providing in said basal medium
(i) a concentration of one or more amino acids reactive with cell transport system A to provide a total amino acid concentration of 5.50 to 20 grams per liter;
(ii) an osmolarity of from 320 to 420 mOsm
said osmolarity being provided by a sodium salt osmolyte.
7. The claim 6 cell culture medium in which the osmolyte (ii) is sodium chloride.
8. The claim 6 cell culture medium in which said one or more amino acids reactive with cell transport system A are zwitterionic amino acids.
9. The claim 6 cell culture medium in which said one or more amino acids reactive with cell transport system A are selected from the group consisting of alanine, glycine, histidine, methionine, proline and serine.
10. The claim 6 or claim 7 or claim 8 cell culture medium in which said basal cell culture medium is RPMI, DMEM, Ham’s F12, RDF or eRDF.
11. The cell culture medium 28101, 28102 or 28103.
12. The method which comprises culturing a cell in the culture medium of claim 1, claim 2, or claim 3, or claim 11.
13. A method which comprises:
(i) providing the cell culture medium of claim 1 or claim 2 or claim 3 or claim 11;
(ii) culturing a CHO cell containing a recombinant expression vector in the medium provided in step (i); and
(iii) recovering the recombinant protein expressed by said CHO cell.
14. The claim 13 method in which the CHO cell containing a recombinant expression vector is a dicistronic DHFR intron expression vector.
15. A method which comprises culturing a CHO cell in culture medium 28103.
16. The method of claim 13 wherein said cell cultured in said culture medium is a hybridoma.
17. The method which comprises culturing a mammalian cell in the culture medium of claim 1, claim 2 or claim 3 or claim 11.
18. The method which comprises culturing a mammalian cell having an expression vector for a recombinant protein in a culture medium of claim 1, claim 2 or claim 3 or claim 11.
19. The method which comprises culturing a CHO cell or a BHK cell or a CO5 cell or a Namaliva cell having expression vector for a recombinant protein in a culture medium of claim 1, claim 2, claim 3 or claim 11.
20. The method which comprises culturing a cell in the culture medium of claim 6 or claim 7 or claim 8 or claim 9.
21. The method which comprises culturing a mammalian cell in the culture medium of claim 7 or claim 8 or claim 9.
22. The method which comprises culturing a mammalian cell having an expression vector for a recombinant protein in a culture medium of claim 7 or claim 8 or claim 9.
23. The method which comprises culturing a CHO cell or a BHK cell or a CO5 cell or a Namaliva cell having expression vector for a recombinant protein in a culture medium of claim 7 or claim 8 or claim 9.
24. A dry mixture of cell culture medium components comprising amino acids, carbohydrates and vitamins,
wherein said amino acids component comprise at least 20% of the dry weight of said mixture.
25. The claim 24 mixture wherein said amino acids component comprises from 30% to 50% of the dry weight of said mixture.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An deployable template structure comprising:
a template including at least one of the following:
a virtual machine (VM) section to describe possible virtual machines deployable on a system;
a filter section to describe one or more filters applicable to components of the system; and
a component configuration settings section to describe properties of deployable engine components.
2. The deployable template structure of claim 1, wherein the virtual machine section of the template includes levels for at least one of the following:
a VM vendor level to describe at least one possible deployable VM vendors;
a platform level to describe at least one possible platform of a specific possible deployable VM vendor; and
a global parameter level to describe parameters applicable to any possible deployable VM vendor and platform.
3. The deployable template structure of claim 1, wherein a filter of the filter section includes:
an action to be performed on a component;
a component type;
a vendor name; and
a component name.
4. The method of claim 3, wherein the action to be performed is selected from the group consisting of:
start; and
stop.
5. The method of claim 4, wherein the component type is selected from the group consisting of:
service;
library;
interface; and
application.
6. The deployable template structure of claim 1, wherein the component configuration settings section includes properties for each type of deployable engine component.
7. The deployable template structure of claim 1, wherein at least one of the sections of the template are XML documents.
8. A method comprising:
acquiring properties of a first configuration profile, the first configuration profile defining a structure of an instance’s configuration values;
acquiring values of a second configuration profile, the second configuration profile further defining values for the structure of the instance’s configuration;
inheriting the properties of the first configuration profile, wherein the second configuration profile inherits the properties of the first configuration profile; and
applying the values of the second configuration profile to the first configuration profile;
acquiring the properties of a third configuration profile, the third configuration profile defining a default template applicable to multiple instances on a single system, wherein the default template includes:
a virtual machine (VM) section to describe possible virtual machines deployable on the system,
a filter section to describe filters applicable to components of the system, and
a component configuration settings section to describe properties of deployable engine components;

the third configuration profile inheriting the properties of the first and second configuration profiles; and
applying the sections of the third configuration profile to the inherited profiles.
9. The method of claim 8, further comprising:
acquiring the properties of a fourth configuration profile, the fourth configuration profile further defining the use case scenario;
inheriting the properties of the first, second, and third configuration profiles, wherein the fourth configuration profile inherits the properties of the first, second, and third configuration profiles; and
applying the values of the fourth configuration profile to the inherited profiles.
10. The method of claim 9, further comprising:
acquiring the properties of a fifth configuration profile, the fifth configuration profile further defining a specific instance;
inheriting the properties of the first, second, third, and fourth configuration profiles, wherein the fifth configuration profile inherits the properties of the first, second, third, and fourth configuration profiles; and
applying the values of the fifth configuration profile to the inherited profiles.
11. The method of claim 8, wherein the configuration profiles are written in XML.
12. An article of manufacture including program code which, when executed by a machine, causes the machine to perform a method, the method comprising:
acquiring properties of a first configuration profile, the first configuration profile defining a structure of an instance’s configuration values;
acquiring values of a second configuration profile, the second configuration profile further defining values for the structure of the instance’s configuration;
inheriting the properties of the first configuration profile, wherein the second configuration profile inherits the properties of the first configuration profile; and
applying the values of the second configuration profile to the first configuration profile;
acquiring the properties of a third configuration profile, the third configuration profile defining a default template applicable to multiple instances on a single system, wherein the default template includes:
a virtual machine (VM) section to describe possible virtual machines deployable on the system,
a filter section to describe filters applicable to components of the system, and
a component configuration settings section to describe properties of deployable engine components;