What is claimed is:
1. A combination comprising a plurality of cDNAs that are differentially expressed in DC, and selected from SEQ ID NOs:1-260 or their complements.
2. The combination of claim 1, wherein each of the cDNAs is downregulated in DC and is selected from SEQ ID NOs:1-29.
3. The combination of claim 1, wherein each of the cDNAs is upregulated in DC and is selected from SEQ ID NOs:30-241.
4. The combination of claim 1, wherein each of the cDNAs is upregulated in mature DC and is selected from SEQ ID NOs:242-257.
5. The combination of claim 1, wherein each of the cDNAs is downregulated in mature DC and is selected from SEQ ID NOs:258-260.
6. The combination of claim 1, wherein the cDNAs are immobilized on a substrate.
7. A high throughput method for detecting differential expression of one or more cDNAs in a sample containing nucleic acids, the method comprising:
(a) hybridizing the substrate of claim 6 with nucleic acids of the sample, thereby forming one or more hybridization complexes;
(b) detecting the hybridization complexes; and
(c) comparing the hybridization complexes with those of a standard, wherein differences between the standard and sample hybridization complexes indicate differential expression of cDNAs in the sample.
8. The method of claim 7, wherein the nucleic acids of the sample are amplified prior to hybridization.
9. The method of claim 7, wherein the sample is from a subject with cancer, infectious disease, autoimmunity, allergy, or graft versus host disease and comparison with a standard identifies or stages the disorder.
10. A high throughput method of screening a plurality of molecules or compounds to identify a ligand which specifically binds a cDNA, the method comprising:
(a) combining the combination of claim 1 with the plurality of molecules or compounds under conditions to allow specific binding; and
(b) detecting specific binding between each cDNA and at least one molecule or compound, thereby identifying a ligand that specifically binds to each cDNA.
11. The method of claim 10 wherein the plurality of molecules or compounds are selected from aptamers, DNA molecules, RNA molecules, peptide nucleic acid molecules, mimetics, peptides, transcription factors, repressors, and regulatory proteins.
12. An isolated cDNA selected from the group consisting of SEQ ID NOs:24-29, 187-202, 205-213, 216-241, 256, 257, 259, and 260.
13. A vector containing the cDNA of claim 12.
14. A host cell containing the vector of claim 13.
15. A method for producing a protein, the method comprising the steps of:
(a) culturing the host cell of claim 14 under conditions for expression of protein; and
(b) recovering the protein from the host cell culture.
16. A protein or a portion thereof produced by the method of claim 15.
17. A high-throughput method for using a protein to screen a plurality of molecules or compounds to identify at least one ligand which specifically binds the protein, the method comprising:
(a) combining the protein of claim 16 with the plurality of molecules or compounds under conditions to allow specific binding; and
(b) detecting specific binding between the protein and a molecule or compound, thereby identifying a ligand which specifically binds the protein.
18. The method of claim 17 wherein the plurality of molecules or compounds is selected from DNA molecules, RNA molecules, peptide nucleic acid molecules, mimetics, peptides, proteins, agonists, antagonists, antibodies or their fragments, immunoglobulins, inhibitors, drug compounds, and pharmaceutical agents.
19. A method of using a protein to produce an antibody, the method comprising:
a) immunizing an animal with the protein of claim 16 under conditions to elicit an antibody response;
b) isolating animal antibodies; and
c) screening the isolated antibodies with the protein, thereby identifying an antibody which specifically binds the protein.
20. A antibody produced by the method of claim 19.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. In a printer having a printhead having a plurality of print nozzles, which use a sonic pulse to discharge drops of ink from said nozzles to form an image during the print cycle, an ink cartridge to supply ink to said nozzles, a method of printing comprising the steps of:
determining the initial volume of ink remaining in the ink cartridge prior to printing;
recording said initial volume in a memory unit;
determining the volume of ink required to print the next page;
printing the next page if there is sufficient ink in the cartridge to print the page;
counting the number of dots of ink discharged in the printing of the page;
obtaining data on the size of a dot under present conditions;
feeding the data on the size of a dot and the number of dots printed to a processor where the number of dots is multiplied by the volume of each dot to determine the volume of ink used in the printing of the page;
feeding data on the volume of ink printed to the memory unit where the volume of ink, printed, is subtracted from the volume of ink in the cartridge prior to printing that page to obtain the volume of ink in the cartridge after printing that page, and
repeating the above steps for each page printed.
2. In a printer the method of printing according to claim 1, wherein the reservoir is a cartridge of known initial volume of ink.
3. In a printer the method of printing according to claim 1 or 2, wherein the reservoir contains separate stores of a plurality of inks, and the volume of each ink is determined after each page is printed.
4. In a printer the method of printing according to claim 1, including the step of sensing properties of the ink and processing that data to determine the volume of the dots discharged from the nozzles.
5. In a printer the method of printing according to claim 4, wherein only temperature of the ink is sensed.
6. A printer comprising:
a printerhead having print nozzles through which dots of ink a discharged to print an image;
an ink cartridge containing ink to supply to said print nozzles;
a processor and memory unit associate with the ink cartridge and containing data on the characteristics of the ink and the volume of ink in the cartridge;
a sensor associated with said printhead to count the number of dots of ink discharged from said nozzles during printing of a page;
a processor unit for extracting data from the processor and memory unit to determine the volume of the an ink dot discharged from the nozzles and in communication with said sensor to receive data on the number of dots printed for the last page, multiplying the dot volume by the number of dots to determine the amount of ink used in printing the page, and inputing this data to the processor and memory unit, to update the volume of ink remaining in the cartridge; said processor unit instructing said printhead to print an image.
7. A printer according to claim 6 wherein the process and memory unit is a QA chip.
8. A printer according to claim 6 wherein the cartridge contains a plurality of different inks, and the volume of each different ink is determined.