1. An aquarium liquid security system, the system comprising:
a liquid detection sensor, attachable to an aquarium enclosure, electrically responsive to liquid; and,
an alarm interface connected to the sensor.
2. The aquarium leak security system of claim 1 wherein the sensor includes a pair of electrically conductive ink traces printed on an aquarium enclosure.
3. The aquarium leak security system of claim 1 wherein the sensor includes:
a liquid-permeable strip of material with a first external surface;
a pair of electrically conductive traces; and,
liquid-permeable adhesive formed on the strip first surface, for attaching the sensor to an aquarium enclosure.
4. The aquarium leak security system of claim 3 wherein the pair of electrically conductive traces are embedded in the strip.
5. The aquarium leak security system of claim 1 wherein the sensor includes:
a transparent dielectric sheet with a surface, attachable to an aquarium enclosure; and,
a pair of electrically conductive traces formed on the transparent dielectric sheet surface.
6. The aquarium leak security system of claim 1 further comprising:
an alarm unit having a sensor interface connected to the sensor’s alarm interface, for creating an alarm signal in response to measuring a predetermined sensor resistance.
7. The aquarium leak security system of claim 6 wherein the alarm unit has an output interface for sending an auxiliary alarm signal in response to measuring the predetermined sensor resistance.
8. The aquarium leak security system of claim 6 wherein the alarm unit has a user interface for selecting the predetermined resistance threshold.
9. An aquarium leak security system, the system comprising:
an aquarium base having a top face attachable to an aquarium enclosure bottom surface, and having sides;
a liquid detection sensor, at least partially wrapped around the sides of the base, electrically responsive to liquid; and,
an alarm interface connected to the sensor.
10. The aquarium leak security system of claim 9 wherein the sensor includes a pair of electrically conductive ink traces printed on the sides of the base.
11. The aquarium leak security system of claim 9 wherein the sensor includes:
a liquid-permeable strip of material with a first external surface;
a pair electrically conductive traces; and,
liquid-permeable adhesive formed on the strip first surface, attaching the sensor to the sides of the base.
12. An aquarium water-level detector system, the system comprising:
a liquid detection sensor, attachable to an aquarium enclosure inside surface, electrically responsive to the absence of water; and,
an alarm interface connected to the sensor.
13. The water-level detection system of claim 12 wherein the sensor includes a pair of conductive ink traces printed on the inside surface of an aquarium enclosure, extending from a top edge of the enclosure towards a bottom edge; and,
wherein the alarm interface is connected to the sensor at the enclosure top edge.
14. The water-level detection system of claim 12 wherein the sensor includes a pair of electrically conductive probes attachable to a top edge of an aquarium enclosure, extendable into the enclosure; and
wherein the alarm interface is connected to the sensor at the aquarium enclosure top edge.
15. The water-level detection system of claim 12 wherein the sensor includes:
a strip of material with a first surface and a second surface;
a pair of electrically conductive traces formed on the strip first surface;
adhesive formed on the strip second surface, for attaching the sensor to an inside surface of an aquarium enclosure, extending from a top edge of the enclosure towards a bottom edge; and,
wherein the alarm interface is connected to the sensor at the enclosure top edge.
16. The water-level detection system of claim 12 further comprising:
an alarm unit having a sensor interface connected to the sensor’s alarm interface, for creating an alarm signal in response to measuring a maximum sensor resistance.
17. The water-level detection system of claim 16 wherein the alarm unit has an output for sending an auxiliary alarm signal in response to measuring the maximum sensor resistance.
18. The water-level detection system of claim 16 wherein the alarm unit has a user interface for selecting the maximum resistance threshold.
19. The water-level detection system of claim 12 wherein the liquid detection sensor includes a pair of tensioned electrical conductors responsive to water pressure, for mounting on an aquarium enclosure inside surface.
20. The water-level detection system of claim 12 wherein the sensor includes:
a transparent dielectric sheet with a surface, attachable to an aquarium enclosure inside surface; and,
a pair of electrically conductive traces formed on the transparent dielectric sheet surface.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for isolating a peptide, comprising:
contacting a first peptide library comprising a first subset of peptides with a first set of nucleotides comprising a heterogeneous population of nucleotides other than a pre-selected nucleotide, to identify a first sub-library of peptides comprising a second subset of peptides that do not bind to the first set of nucleotides;
contacting the first sub-library of peptides with a homogeneous population of the pre-selected nucleotide, to identify a second sub-library of peptides that bind to the pre-selected nucleotide; and
isolating at least one peptide from the second sub-library of peptides;
wherein the first peptide library comprises a sub-library preselected from an initial peptide library by at least two rounds of biopanning.
2. (canceled)
3. The method of claim 1, wherein the at least two rounds of biopanning comprise two contacting steps with two different ligand populations, wherein the first ligand population comprises a homogeneous population of homo-oligonucleotides that comprise nucleotides other than the pre-selected nucleotide, and the second ligand population comprises a homogeneous population of homo-oligonucleotides comprising the pre-selected nucleotide and no other nucleotides.
4. The method of claim 3, wherein the first round of biopanning comprises amplifying the peptides that do not bind the first ligand population and the second round of biopanning comprises amplifying the peptides that bind the second ligand population.
5. The method of claim 4, wherein the first peptide library comprising the first subset of peptides comprises the population of peptides that bind the second ligand population.
6. The method of claim 4, further comprising an initial round of biopanning prior to the at least two round of biopanning, wherein the initial round comprises contacting the initial peptide library with a heterogeneous population of single stranded oligonucleotides to identify an initial sub-library of peptides that bind single stranded nucleic acid oligonucleotides.
7. The method of claim 6, wherein the initial sub-library is subjected to the at least two rounds of biopanning.
8. The method of claim 6, wherein the initial sub-library is separated from the initial peptide library prior to the at least two rounds of biopanning.
9. A peptide isolated by the method of claim 1.
10. A peptide isolated by the method of claim 2.
11. A peptide isolated by the method of claim 8
12. The method of claim 8, wherein the separation step is carried out with a solid phase.
13. The method of claim 12, wherein the solid phase comprises magnetic particles.
14. The method of claim 1, wherein the nucleotides that contact the library and sub-library are aminolated.
15. The method of claim 1, wherein the first peptide library comprises an encoded cyclic peptide library.
16. The method of claim 1, wherein the first peptide library comprises a cyclic peptide phage display library.
17. The method of claim 1, wherein the pre-selected nucleotide comprises a non-natural or a natural nucleotide.
18. The method of claim 3, wherein the homo-oligonucleotides comprise of five to ten nucleotides.
19. A peptide library comprising the second sub-library of peptides of claim 1.
20. A peptide isolated by the method of claim 1, the peptide comprising SEQ ID NOs 1-34.
21. A method for identifying a peptide comprising:
incubating a first peptide library with a first set of nucleic acid oligonucleotides to identify a first sub-library of peptides that bind the first set of nucleic acid oligonucleotides;
separating the first sub-library from the first library;
contacting the first sub-library with a second set of nucleic acid oligonucleotides comprising homo-oligomers of nucleotides other than a preselected nucleotide, to identify a second sub-library of peptides that do not bind the second set of nucleic acid oligonucleotides;
separating the second sub-library from the first sub-library;
contacting the second sub-library with a third subset of nucleic acid oligonucleotides comprising a homogeneous population of homo-oligonucleotides comprising the pre-selected nucleotide and no other nucleotides, to identify a third sub-library of peptides that bind to the third subset of nucleic acid oligonucleotides;
separating the third sub-library from the second sub-library;
contacting the third sub-library with a plurality of a first set of aminolated nucleotides comprising a heterogeneous population of nucleotides other than the pre-selected nucleotide, to identify a fourth sub-library of peptides that do not bind the first set of aminolated nucleotides;
separating the fourth sub-library from the fifth sub-library; and
contacting the fifth sub-library with a second set of aminolated nucleotides, comprising a homogeneous population of the pre-selected nucleotide, to produce a sixth sub-library of peptides that bind the second set of aminolated nucleotides, thereby identifying a peptide that binds the pre-selected nucleotide.