1. A method of making sticky powder comprising
mixing one or more resin or (co)polymer powders in one or more mixing devices without agglomerating the said powders and while measuring the power or torque drawn by the said mixing devices, said mixing continuing until the measure of the said power or torque drawn indicates that the said powders have become sticky.
2. A method as claimed in claim 1, wherein said mixing further comprises adding to the said powders one or more dry materials and mixing to form a sticky powder mixture so that the said dry materials adhere to the said sticky powders.
3. A method as claimed in claim 1, further comprising
slowing or stopping the said mixing or cooling while mixing the said sticky powders once the said sticky powders have been formed,
adding one or more dry materials to form a sticky powder mixture, and,
further mixing to bond the said sticky powders and the said dry materials together.
4. A method as claimed in claim 3, wherein the said one or more dry materials comprise one or more (co)polymers or resins.
5. A method as claimed in claim 2, wherein the said one or more dry materials comprise one or more of each of flake materials, layered pigments, layered clays, catalysts, antimicrobials, cyroprocessed materials, freeze-dried materials, and any material encapsulated or dispersed in brittle materials.
6. A method as claimed in claim 3, wherein the said one or more dry materials comprise one or more of each of flake materials, layered pigments, layered clays, catalysts, antimicrobials, cyroprocessed materials, freeze-dried materials, and any material encapsulated or dispersed in brittle materials.
7. A method as claimed in any one of claims 5 or 6, wherein the said one or more dry materials comprise one or more leafing metallic flake materials, one or more non-leafing metallic flake materials or mixtures thereof.
8. A method as claimed in claim 7, wherein the said one or more metallic flake materials comprise non-leafing aluminum flake, leafing aluminum flake materials, or mixtures thereof.
9. A method as claimed in any one of claims 1 to 6, wherein the method is fully automated for batch or for continuous processing.
10. A method as claimed in claim 9, wherein the method is fully automated for controlling the said method in-process.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1.-114. (canceled)
115. An isolated nutritive protein comprising a first polypeptide comprising a first polypeptide sequence, wherein the first polypeptide sequence comprises: a. a ratio of branch chain amino acid residues to total amino acid residues of at least 24%; b. a ratio of Leu residues to total amino acid residues of at least 11%; and c. a ratio of essential amino acid residues to total amino acid residues of at least 49%, and wherein the first polypeptide sequence comprises at least one of: a. at least 95% homology to at least 50 amino acids of naturally occurring nutritive protein, b. less than 50 to 90% global homology to a known allergen, or c. at least 20, 50, or 100 amino acids in length.
116. The isolated nutritive protein of claim 115, wherein the first polypeptide sequence comprises a calculated solvation score of \u221220 or less and a calculated aggregation score of 0.75 or less.
117. The isolated nutritive protein of claim 115, wherein the first polypeptide sequence comprises an amino acid sequence selected from: i. an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760; ii. a modified derivative of an amino acid sequence selected from SEQ ID NO: I to SEQ ID NO: 760; and iii. a mutein of an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760.
118. The isolated nutritive protein of claim 115, wherein the first polypeptide sequence consists of an amino acid sequence selected from: i. an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760, ii. a modified derivative of an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760, and iii. a mutein of an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760.
119. The isolated nutritive protein of claim 115, wherein the first polypeptide sequence is at least 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 99.5% homologous to at least one amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 760.
120. A nutritive composition comprising at least one isolated nutritive protein of claim 115, wherein at least one isolated nutritive protein has a simulated gastric digestion half-life shorter than 60 minutes, andor wherein at least one isolated nutritive protein has an aqueous solubility at pH 7 of at least 12.5 gL, andor wherein at least one isolated nutritive protein is present in the nutritive composition in an amount of about 0.1 g, 0.1-1 g, 1 g, 2 g, 3 g, 4 g, 5 g, 6 g, 7 g, 8 g, 9 g, 10 g, 15 g, 20 g, 25 g, 30 g, 35 g, 40 g, 45 g, 50 g, 55 g, 60 g, 65 g, 70 g, 75 g, 80 g, 85 g, 90 g, 95 g, and 100 g.
121. A recombinant microorganism comprising a vector comprising a nucleic acid sequence that encodes a nutritive protein according to claim 115, and further comprising an expression control sequence operatively linked to the nucleic acid sequence that encodes the nutritive protein.
122. A pharmaceutical composition comprising the nutritive composition of claim 120 formulated for oral administration to a mammalian subject for maintaining andor improving muscle function andor muscle gain, in subjects suffering from muscular deterioration, wherein at least one isolated nutritive protein is present in the nutritive composition in an amount of at least about 0.1 g, wherein the pharmaceutical composition further optionally comprises at least one component selected from a protein, a polypeptide, a peptide, a free amino acid, a carbohydrate, a lipid, a mineral or mineral source, a vitamin, a supplement, an organism, a pharmaceutical, and an excipient.
123. A pharmaceutical composition comprising the nutritive composition of claim 120 formulated for oral administration to a mammalian subject for reducing lean tissue loss, wherein at least one isolated nutritive protein is present in the nutritive composition in an amount of at least about 0.1 g, wherein the pharmaceutical composition optionally further comprises at least one component selected from a protein, a polypeptide, a peptide, a free amino acid, a carbohydrate, a lipid, a mineral or mineral source, a vitamin, a supplement, an organism, a pharmaceutical, and an excipient.
124. A method of making a nutritive protein of claim 115, comprising isolating the nutritive protein from a recombinant microorganism.
125. A method of maintaining or increasing at least one of muscle mass, muscle strength, or functional performance in a subject, or reducing lean tissue loss in a subject, or achieving a desirable body mass index in a subject, the method comprising providing to the subject a sufficient amount of a nutritive protein of claim 115, wherein the subject is optionally at least one of elderly, critically-medically ill, frail, obese, or suffering from protein-energy malnutrition, cachexia, sarcopenia, osteoporosis, chronic obstructive pulmonary disease, chronic heart failure, HIV, or other disease states.
126. The method of claim 125, wherein the nutritive protein, nutritive composition or pharmaceutical composition is consumed at a rate of from 0.1 g to 1 g a day, 1 g to 5 g a day, from 2 g to 10 g a day, from 5 g to 15 g a day, from 10 g to 20 g a day, from 15 g to 30 g a day, from 20 g to 40 g a day, from 25 g to 50 g a day, from 40 g to 80 g a day, from 50 g to 100 g a day, or more.
127. The method of claim 125, wherein the nutritive protein, nutritive composition or pharmaceutical composition comprises at least about 5% of the total protein intake by the subject over a dietary period, andor nutritive protein or nutritive composition accounts for at least about 5% of the total calorie intake by the subject over a dietary period.
128. The method of claim 125, wherein the nutritive protein or nutritive composition is provided in a sufficient amount to induce in an obese subject a satiation response andor a satiety response in the subject.