1. A gene recombination vector containing an expression cassette for enhancing photosynthesis activity, comprising a DNA fragment comprising a gene encoding a protein having FBPase andor SBPase activities between a Rubisco large subunit gene and an acetyl CoA carboxylase subunit gene.
2. The vector as claimed in claim 1, wherein the protein having FBPase activity is any one of the followings;
(a) a protein comprising an amino acid sequence described in SEQ ID NO: 1 of Sequence Listing;
(b) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, added or inserted in SEQ ID NO: 1 of Sequence Listing, and having FBPase activity; and
(c) a protein having at least 60% or more homology to an amino acid sequence described in SEQ ID NO: 1 of Sequence Listing, and having FBPase activity.
3. The vector as claimed in claim 1, wherein the gene encoding a protein having FBPase activity is a gene comprising any one of the following DNAs;
(a) DNA comprising a nucleotide sequence described in SEQ ID NO: 2 of Sequence Listing;
(b) DNA comprising a nucleotide sequence in which one or several bases are deleted, substituted, added or inserted in SEQ ID NO: 2 of Sequence Listing, and encoding a protein having FBPase activity;
(c) DNA which hybridizes with DNA comprising a nucleotide sequence complementary to DNA comprising a nucleotide sequence described in SEQ ID NO: 2 of Sequence Listing under stringent conditions, and comprises a nucleotide sequence encoding a protein having FBPase activity; and
(d) DNA having at least 60% or more homology to DNA comprising a nucleotide sequence described in SEQ ID NO: 2 of Sequence Listing, and comprising a nucleotide sequence encoding a protein having FBPase activity.
4. The vector as claimed in claim 1, wherein the protein having SBPase activity is any one of the following proteins;
(a) a protein comprising an amino acid sequence described in SEQ ID NO: 3 of Sequence Listing;
(b) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, added or inserted in SEQ ID NO: 3 of Sequence Listing, and having SBPase activity; and
(c) a protein having at least 60% or more homology to an amino acid sequence described in SEQ ID NO: 3 of Sequence Listing, and having SBPase activity.
5. The vector as claimed in claim 1, wherein the gene encoding a protein having SBPase activity is a gene comprising any one of the following DNAs;
(a) DNA comprising a nucleotide sequence described in SEQ ID NO: 4 of Sequence Listing;
(b) DNA comprising a nucleotide sequence in which one or several bases are deleted, substituted, added or inserted in SEQ ID NO: 4 of Sequence Listing, and encoding a protein having SBPase activity;
(c) DNA which hybridizes with DNA comprising a nucleotide sequence complementary to DNA comprising a nucleotide sequence described in SEQ ID NO: 4 of Sequence Listing under stringent conditions, and comprises a nucleotide sequence encoding a protein having SBPase activity; and
(d) DNA having at least 60% or more homology to DNA comprising a nucleotide sequence described in SEQ ID NO: 4 of Sequence Listing, and comprising a nucleotide sequence encoding a protein having SBPase activity.
6. The vector as claimed in claim 1, wherein the protein having FBPase and SBPase activities is any one of the followings:
(a) a protein comprising an amino acid sequence described in SEQ ID NO: 5 of Sequence Listing;
(b) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, added or inserted in SEQ ID NO: 5 of Sequence Listing; and having FBPase and SBPase activities; and
(c) a protein having at least 60% or more homology to an amino acid sequence described in SEQ ID NO: 5 of Sequence Listing; and having FBPase and SBPase activities.
7. The vector as claimed in claim 1, wherein the gene encoding a protein having FBPase and SBPase activities is a gene comprising any one of the following DNAs;
(a) DNA comprising a nucleotide sequence described in SEQ ID NO: 6 of Sequence Listing;
(b) DNA comprising a nucleotide sequence in which one or several bases are deleted, substituted, added or inserted in SEQ ID NO: 6 of Sequence Listing, and encoding a protein having FBPase and SBPase activities;
(c) DNA which hybridizes with DNA comprising nucleotide sequence complementary to a DNA comprising a nucleotide sequence described in SEQ ID NO: 6 of Sequence Listing under stringent conditions, and comprises a nucleotide sequence encoding a protein having FBPase and SBPase activities; and
(d) DNA having at least 60% or more homology to DNA comprising a nucleotide sequence described in SEQ ID NO: 6 of Sequence Listing, and comprising a nucleotide sequence encoding a protein having FBPase and SBPase activities.
8. The vector as claimed in claim 1, wherein the expression cassette has a ribosome-binding site upstream of a translation initiation point of a DNA fragment comprising a gene encoding a protein having FBPase andor SBPase activities.
9. The vector as claimed in claim 8, wherein the expression cassette has a promoter upstream of a ribosome-binding site, and a terminator downstream of DNA fragment comprising a gene encoding a protein having FBPase andor SBPase activities.
10. The vector as claimed in claim 9, wherein the promoter and the terminator are a promoter and a terminator derived from tobacco chloroplasts, respectively.
11. The vector as claimed in claim 1, wherein the Rubisco large subunit gene and the acetyl CoA carboxylase subunit gene are genes derived from tobacco, respectively.
12. A recombinant gene vector comprising an expression cassette containing a DNA fragment comprising a gene encoding a protein having FBPase andor SBPase activities between a tobacco-derived Rubisco large subunit gene and an acetyl CoA carboxylase subunit gene, having a ribosome-binding site upstream of a translation initiation point of the DNA fragment, having a tobacco-derived promoter between a Rubisco large subunit gene and a ribosome-binding site, and having a tobacco-derived terminator between the acetyl CoA carboxylase subunit gene and the DNA fragment.
13. A transformed chloroplast characterized in that the vector according to claim 1 is introduced into chloroplasts.
14. A plant containing transformed chloroplasts according to claim 13.
15. The plant as claimed in claim 14, wherein the plant is tobacco.
16. A plant having 2-fold or higher FBPase activity compared to the original one, characterized in that a FBPaseSBPase gene is introduced into the chloroplast genome of higher plants and expressed using a chloroplast transformation technique.
17. A plant having two-fold or higher enhanced photosynthesis rate as compared with the wild variety, characterized in that a FBPaseSBPase gene is introduced into the chloroplast genome of higher plants using a vector according to claim 1, followed by expression.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A computer mouse that is attachable to the hand of the computer operator and cradled within the palm of the operator’s hand for controlling the movement of the cursor on the video display screen of the computer, comprising:
a housing having a front portion, an opposite rear portion, an upper surface and an opposite lower surface;
the upper surface having a curvilinear contour for accommodating the palm of the operator’s hand;
the lower surface having a sloping contour for facilitating the gripping of the housing by the operator’s fingers;
a computer mouse ball located at the front portion of the housing and manually controlled by the thumb of the operator;
a left click button mounted adjacent to the front portion and manipulated by the index finger of the operator for controlling certain computer functions in conjunction with the positioning of the cursor on the screen;
a right click button mounted adjacent the left click button and manipulated by the middle finger of the operator for controlling certain computer functions conjunction with the positioning of the cursor on the screen; and
means to removably fasten the computer mouse to the hand of the operator and within the palm of the operator’s hand.
2. The computer mouse of claim 1 wherein the means for fastening the mouse to the operator’s hand includes a flexible strap that extends from the front portion to the rear portion of the housing and through which the operator’s hand is passed so that the operator can grip the computer mouse in the palm of his hand.
3. The computer mouse of claim 2 further comprising an adapter for interconnecting both the computer mouse and a conventional computer mouse to the computer.
4. The computer mouse of claim 3 wherein the mouse is able to make a wireless interconnection to the computer for controlling cursor movement by communication with a wireless receiver.
5. A computer mouse that is attachable to the hand of a computer user and cradled within the palm of the user for controlling the movements of the cursor on the video display screen of the computer and other computer functions, comprising:
a housing having a front portion, an opposite rear portion, an upper surface and an opposite lower surface;
the upper surface having a sloping contour for accommodating the palm of the user’s hand;
the lower surface having a sloping contour for facilitating the comfortable gripping of the housing by the user’s fingers;
a computer mouse ball located adjacent the front portion of the housing and manipulated by the user’s thumb with the mouse ball initiating certain computer functions such as the click and drag functions;
a left click button mounted on the housing adjacent the front portion and manipulated by the user’s index finger for controlling computer functions coincident with the movement and positioning of the cursor on the screen;
a right click button mounted on the housing adjacent the left click button and manipulated by the user’s middle finger for controlling computer functions coincident with the movement and positioning of the cursor on the computer screen; and
means to fasten the computer mouse to the user’s hand and within the palm of the user so that the computer mouse moves with the natural movements of the user’s hand for moving and controlling the positioning of the cursor on the video display screen and is not in contact with the work surface.
6. The computer mouse of claim 5 wherein the means to fasten the mouse to the hand of the user includes a flexible strap that extends from the front portion to the rear portion of the housing and through which the user’s hand is slipped for gripping and cradling the mouse within the user’s palm.
7. The computer mouse of claim 6 further comprising an adapter for interconnecting both the computer mouse and a conventional mouse to the computer so that the user can select which mouse to use for a given computer function.
8. The computer mouse of claim 7 further comprising a wireless configuration whereby the computer mouse interfaces with the computer through a wireless receiver for controlling the movement and positioning of the cursor and other computer functions through manipulation of the mouse ball and the left and right click buttons.