What is claimed is:
1. An immunoassay using an insoluble carrier particle which comprises
(i) using an insoluble magnetic carrier particle in a state substantially free of any adsorbed antigen andor antibody,
(ii) adsorbing an antigenic substance in a test sample on said insoluble magnetic carrier particle or binding the antigenic substance to the said insoluble magnetic carrier particle,
(iii) reacting the resultant insoluble magnetic carrier particle from the above treatment (ii) with an antibody being of a labeled antibody specifically reactive with some of the said antigenic substance, said antibody being specifically reactive with a solid-phase form of the antigenic substance, but substantially non-reactive with a native-state form of the antigenic substance, wherein said solid-phase form antigenic substance is attached to the said insoluble carrier particle, and said native-state antigenic substance is present in a liquid phase, and
(iv) measuring as an indicator the label on the resultant labeled antibody captured by the said solid-phase antigenic substance.
2. The immunoassay according to claim 1, wherein said immunoassay comprises
(A) adsorbing said antigenic substance in said test sample on said insoluble magnetic carrier particle or binding the said antigenic substance to the insoluble magnetic carrier particle, and
(B) then reacting the captured antigenic substance with said specifically solid phase antigenic substance-reactive antibody without removing the said test sample with washing.
3. The immunoassay according to claim 1 or 2, wherein said immunoassay comprises
(a) reacting said labeled antibody with the insoluble magnetic carrier particle that said antigenic substance in said test sample is adsorbed on or bound to,
(b) then separating an unreacted labeled antibody from the insoluble magnetic carrier particle in the presence of a magnetic field action, and
(c) measuring as an indicator the label on the resultant labeled antibody captured by the said solid-phase antigenic substance.
4. The immunoassay according to any of claims 1 to 3, wherein said antibody is monoclonal.
5. The immunoassay according to any of claims 1 to 3, wherein said antibody is polyclonal.
6. The immunoassay according to any of claims 1 to 5, wherein said insoluble magnetic carrier particle is selected from fine particles wherein said fine particle is substantially insoluble in an aqueous liquid medium and comprised of an organic polymer material phase and a magnetic material phase.
7. The immunoassay according to any of claims 1 to 6, wherein said insoluble magnetic carrier particle is selected from fine particles wherein said fine particle comprises not only a coat phase made up of one or more organic polymer materials but also a core phase made up of one or more magnetic materials.
8. The immunoassay according to any of claims 1 to 7, wherein said insoluble magnetic carrier particle is selected from latex particles wherein said latex particle has (a) an average particle size ranging from 0.01 to 20 microns and (b) a core made up of one or more magnetic materials.
9. The immunoassay according to any of claims 1 to 7, wherein said insoluble magnetic carrier particle is selected from latex particles wherein said latex particle has an average particle size ranging from 0.1 to 6 microns and a core comprised of one or more magnetic materials.
10. The immunoassay according to any of claims 1 to 9, wherein said immunoassay is practicable in an automated fashion from dispensing said test sample to attaining test results with a clinical chemistry autoanalyzer suited for magnetic particles.
11. The immunoassay according to any of claims 1 to 10, wherein the antigenic substance in the test sample is HbA1c.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for dyeing keratinous fibers, comprising treating said fibers with:
i) at least one entity chosen from compound chosen from synthetic or natural compounds of formulae (I) and (II), their mesomeric forms, their stereoisomers, their addition salts with an acid or base that is cosmetically acceptable, and their hydrates:
and wherein:
represents a single or conjugated double carbon-carbon bond;
X represents either:
R1, R2, R3, R4, R5 and R6, which are identical or different, are chosen from a hydrogen atom, a hydroxy group, an optionally substituted alkyl group, optionally substituted alkoxy, and an optionally substituted acyloxy group;
ii) at least one metal salt,
iii) hydrogen peroxide or at least one system which generates hydrogen peroxide, and
iv) at least one (bi)carbonate.
2. The dyeing method of claim 1, wherein the at least one entity is chosen from naturally occurring compounds of formulae (I) and (II).
3. The dyeing method of claim 1, wherein the radical R6 of formulae (I) and (II) represents a hydroxyl group.
4. The dyeing method of claim 1, wherein the radical R1 of formulae (I) and (II) represents a hydrogen atom or a hydroxyl group.
5. The dyeing method of claim 1, wherein the at least one entity is a compound chosen from hematoxylin, brazilin, hematein, brazilein their mesomeric forms, their stereoisomers, their addition salts with an acid or base that is cosmetically acceptable, and also the hydrates, with hematoxylin, brazilin, hematein, and brazilein having the structures:
6. The dyeing method of claim 1, wherein the at least one entity is chosen from the compounds of formula (I).
7. The dyeing method of claim 1, wherein the at least one entity is provided in the form of a plant extract chosen from extracts of Haematoxylon campechianum, Haematoxylon brasiletto, Caesalpinia echinata, Caesalpinia sappan, Caesalpinia spinosa and Caesalpina brasiliensis.
8. The dyeing method of claim 1, wherein the at least one metal salt is chosen from Mn and Zn salts.
9. The dyeing method of claim 8, wherein the Mn and Zn salts are chosen from halides, sulfates, phosphates, nitrates. perchlorates, salts of carboxylic acids, and polymeric salts.
10. The dyeing method of claim 1, wherein the at least one metal salt is chosen from Mn and Zn oxides.
11. The dyeing method of claim 1, wherein the hydrogen peroxide or at least one system which generates hydrogen peroxide is chosen from:
a) urea peroxide;
b) polymeric complexes which release hydrogen peroxide;
c) oxidases which produce hydrogen peroxide in the presence of an appropriate substrate;
d) metal peroxides which, in water, generate hydrogen peroxide;
e) perborates;
f) percarborates; and
g) hydrogen peroxide.
12. The dyeing method of claim 1, wherein the at least one (bi)carbonate is chosen from alkali metal (bi)carbonates and alkaline earth metal (bi)carbonates.
13. The method of claim 1, wherein the method comprises a stage consisting of applying, to the keratinous fibers, a cosmetic composition comprising the at least one entity, the at least one metal salt, the hydrogen peroxide or the at least one system which generates hydrogen peroxide, and the at least one (bi)carbonate.
14. The dyeing method of claim 1, wherein the method comprises at least first and second stages, the first stage consisting of applying, to the keratinous fibers, a cosmetic composition comprising the at least one entity, the at least one metal salt, and the hydrogen peroxide or the at least one system which generates hydrogen peroxide, and the second stage consisting of applying a cosmetic composition comprising the at least one (bi)carbonate.
15. The dyeing method of claim 1, wherein the method comprises at least first and second stages, the first stage consisting of applying, to the keratinous fibers, a cosmetic composition comprising the at least one entity and the at least one metal salt, and the second stage consisting of applying a cosmetic composition comprising the hydrogen peroxide or the at least one system which generates hydrogen peroxide and the at least one (bi)carbonate.
16. The method of claim 1, further comprising, just before applying the at least one (bi)carbonate, at least one of:
a) mechanically wiping the keratinous fibers; and
b) drying the keratinous fibers by heating.
17. The method of claim 16, wherein the keratinous fibers are not rinsed between the mechanical wiping and applying the at least one (bi)carbonate.
18. The dyeing method of claim 1, wherein the keratinous fibers comprise pre-wetted hair.
19. The dyeing method of claim 1, wherein the keratinous fibers are treated with an aqueous composition comprising at least one of the at least one entity, the at least one metal salt, the hydrogen peroxide or the at least one system which generates hydrogen peroxide, and the at least one (bi)carbonate.
20. A cosmetic dyeing composition comprising:
i) at least one entity chosen from compounds chosen from synthetic or natural compounds of formulae (I) and (II), their mesomeric forms, their stereoisomers, their addition salts with an acid or base that is cosmetically acceptable, and their hydrates:
and wherein:
represents a single or conjugated double carbon-carbon bond;
X represents either:
R1, R2, R3, R4, R5 and R6, which are identical or different, are chosen from a hydrogen atom, a hydroxy group, an optionally substituted alkyl group, optionally substituted alkoxy, and an optionally substituted acyloxy group;
ii) at least one metal salt,
iii) hydrogen peroxide or at least one system which generates hydrogen peroxide, and
iv) at least one (bi)carbonate.
21. A multicompartment device comprising from 2 to 5 compartments comprising from 2 to 5 compositions in which the ingredients
i) at least one entity chosen from compounds chosen from synthetic or natural compounds of formulae (I) and (II), their mesomeric forms, their stereoisomers, their addition salts with an acid or base that is cosmetically acceptable, and their hydrates:
and wherein:
represents a single or conjugated double carbon-carbon bond;
X represents either:
R1, R2, R3, R4, R5 and R6, which are identical or different, are chosen from a hydrogen atom, a hydroxy group, an optionally substituted alkyl group, optionally substituted alkoxy, and an optionally substituted acyloxy group;
ii) at least one metal salt,
iii) hydrogen peroxide or at least one system which generates hydrogen peroxide, and
iv) at least one (bi)carbonate,
and optionally water are distributed.