1. A polypeptide comprising at least two heterologous domains, wherein a first domain is a thermally stable sequence non-specific double-stranded nucleic acid binding domain and the second domain is a thermally stable polymerase domain that has polymerase activity, wherein the first domain specifically binds to polyclonal antibodies generated against either Sso7d (SEQ ID NO:2) or Sac7d (amino acids 7-71 of SEQ ID NO:10).
2. The polypeptide of claim 1, wherein the polymerase domain is from a family A polymerase.
3. The polypeptide of claim 1, wherein the polymerase domain is from a family B polymerase.
4. The polypeptide of claim 1, wherein the polymerase domain is from a Thermus sp. polymerase.
5. The polypeptide of claim 1, wherein the polymerase domain is from a Pyrococcus sp. polymerase.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A transfer device comprising:
a transfer roller that is rotatably provided facing an image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is made of an elastic material,
wherein the material used for the regulating roller has hardness that is higher than a portion of the transfer roller that presses the image carrying member.
2. The transfer device according to claim 1, wherein the regulating roller has a diameter that is larger than a diameter of the transfer roller.
3. The transfer device according to claim 1, wherein the material used for the regulating roller is an insulation material.
4. A transfer device comprising:
a transfer roller that is rotatably provided facing an image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is made of an elastic material,
wherein the transfer roller includes a rotating shaft that protrudes from both ends of the transfer roller in directions away from the transfer roller with respect to an axial direction of the transfer roller,
wherein the urging device urges the rotating shaft to allow the transfer roller to press the image carrying member, and
wherein the regulating roller is provided to the rotating shaft so as to be opposite to the transfer roller while a portion of the rotating shaft that is urged by the urging device is sandwiched therebetween.
5. A transfer device comprising:
a transfer roller that includes a rotating shaft, which faces an image carrying member, at its center, and is capable of rotating about the rotating shaft;
an urging device that urges the rotating shaft in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to the rotating shaft so as to be opposite to the transfer roller while a portion of the rotating shaft that is urged by the urging device is sandwiched therebetween, the regulating roller pressing the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member.
6. A transfer device comprising:
a transfer roller that includes a rotating shaft, which faces an image carrying member, at its center, and is capable of rotating about the rotating shaft;
an urging device that urges the rotating shaft in a direction that the transfer roller presses the image carrying member; and
a pressing device that presses the rotating shaft in a direction reverse to the direction that the urging device urges the rotating shaft, when the urging device urges the rotating shaft.
7. The transfer device according to claim 6, wherein the pressing device presses the rotating shaft at a position opposite to the transfer roller while a portion of the rotating shaft that is urged by the urging device is sandwiched therebetween.
8. An image forming apparatus comprising:
an image carrying member;
a developing device that supplies a developing agent to the image carrying member;
a transfer roller that is rotatably provided facing the image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is made of an elastic material,
wherein the material used for the regulating roller has hardness that is higher than a portion of the transfer roller that pressed the image carrying member.
9. An image forming apparatus comprising:
a plurality of image carrying members;
a plurality of developing devices, each of which supplies a developing agent to each of the respective plurality of image carrying members;
a plurality of transfer devices, each of which includes:
a transfer roller that is rotatably provided facing the corresponding one of the plurality of image carrying members; and
an urging device that urges the transfer roller in a direction that the transfer roller presses the corresponding one of the plurality of image carrying members; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the corresponding one of the plurality of image carrying members to regulate a pressing force of the transfer roller that presses the corresponding one of the plurality of image carrying members, wherein the regulating rollers are made of an elastic material,
wherein the material used for the regulating roller has hardness that is higher than a portion of the transfer roller that pressed the image carrying member.
10. A transfer device comprising:
a transfer roller that is rotatably provided facing an image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is elastically biased when pressing the transfer roller against the image carrying member,
wherein the regulating roller includes at least one biasing member that elastically biases an outer surface of the regulating roller.
11. The transfer device according to claim 10, wherein the material used for the regulating roller includes elastic and inelastic materials.
12. The transfer device according to claim 10 wherein the material used for the regulating roller is an insulation material.
13. The transfer device according to claim 10, wherein the regulating roller includes a material having variable elasticity.
14. A transfer device comprising:
a transfer roller that is rotatably provided facing an image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulate a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is elastically biased when pressing the transfer roller against the image carrying member,
wherein the material used for the regulating roller is a semiconducting material.
15. A transfer device comprising:
a transfer roller that is rotatably provided facing an image carrying member;
an urging device that urges the transfer roller in a direction that the transfer roller presses the image carrying member; and
a regulating roller that is provided to a shaft commonly used with the transfer roller and presses the image carrying member to regulating a pressing force of the transfer roller that presses the image carrying member, wherein the regulating roller is elastically biased when pressing the transfer roller against the image carrying member,
wherein the material used for the regulating roller is a conducting material.