1-34. (canceled)
35. A method of tracking the location of features of a specimen across a set of images of the specimen captured at different reference points, the set of images comprising a plurality of cycles of images, each cycle comprising different images, each image comprising signals having a single color, wherein the signal color is different for each of the different images, said method comprising the steps of:
(a) selecting a subset of images, wherein the images of the subset depict signals corresponding to features of the specimen, and wherein the images of the subset are captured at different reference points;
(b) selecting a primary image from the subset of images, comprising:
(i) selecting the different color images from a single cycle within the subset of images,
(ii) merging signals from at least two different color images of each cycle into a candidate primary image for said cycle,
(iii) repeating steps (i) and (ii), thereby forming a plurality of candidate primary images, and
(iv) selecting the candidate primary image from among the candidate primary images that includes the most signals, thereby obtaining a primary image;
(c) registering the signals depicted in the images of the subset of images with the signals depicted in the primary image, so as to determine the location of the signals depicted in the images with respect to each other, thereby producing signal clumps;
(d) selecting a signal from each of said signal clumps, thereby forming a template that permits the identification of the locations of features of said specimen; and
(e) registering remaining images in said set of images with said template.
36. The method of claim 35, wherein each cycle comprises four different images.
37. The method of claim 35, wherein said specimen comprises at least one tile comprising an array of molecules.
38. The method of claim 37, wherein said specimen comprises a plurality of tiles.
39. The method of claim 37, wherein said array of molecules comprises a plurality of features.
40. The method of claim 39, wherein a feature of said plurality of features comprises multiple copies of a molecule of said array of molecules.
41. The method of claim 40, wherein said molecule comprises a nucleic acid.
42. The method of claim 41, further comprising providing a labeled nucleotide base to said array of molecules, thereby extending a primer hybridized to a nucleic acid within a feature so as to produce a signal corresponding to a feature comprising said nucleic acid.
43. The method of claim 42, wherein the different color corresponds to a different nucleotide base.
44. The method of claim 43, wherein each image of the set of images comprises signals having a single color selected from at least four different colors.
45. The method of claim 44, wherein four different labeled nucleotide bases are provided to said array of molecules so as to produce four different images, thereby producing a cycle of four color images that corresponds to the four possible nucleotides present at a particular position in said nucleic acid.
46. The method of claim 45, wherein said primary image comprises either a single image or a compilation of multiple merged images.
47. The method of claim 35, further comprising the step of selecting a secondary image, wherein said secondary image is the candidate primary image that includes the second most signals.
48. The method of claim 47, wherein said secondary image comprises either a single image or a compilation of multiple merged images.
49. The method of claim 48, further comprising registering the signals from the secondary image with the signals from the primary image.
50. The method of claim 49, further comprising registering signals from nonselected images with the signals from the secondary image, wherein said nonselected images are obtained from the image cycle from which the primary image was obtained.
51. The method of claim 50, further comprising registering signals from remaining images in the subset with signals from the primary image.
52. The method of claim 35, wherein the signals from at least two different color images are subject to cross-talk, and wherein cross-talk between two signal channels allows signals from one color image to appear in the other color image.
53. The method of claim 43, wherein the signals from at least two different color images comprise signals corresponding to A and C.
54. The method of claim 35, wherein said template that permits the identification of the locations of features of said specimen is saved to a locations file.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A paper with a grammage of 10-100 gm2 comprising:
at least 20 wt. % of microfilaments, and
at least 20 wt. % of a non-resinous binder, wherein
the microfilaments have an average filament length in the range of 2-25 mm and a titer less than 1.3 dtex, and
the non-resinous binder comprise at least one of fibrids or pulp.
2. The paper according to claim 1, wherein the paper comprises at least 20% of fibrids the non-resinous binder.
3. The paper according to claim 1, wherein the paper comprises at least 20% of pulp as the non-resinous binder.
4. The paper according to claim 1, wherein the paper comprises at least 20% of cellulose pulp as the non-resinous binder.
5. The paper according to claim 1, wherein the microfilaments are aramid microfilaments.
6. The paper according to claim 1, wherein the paper comprises aramid fibrids as the non-resinous binder.
7. The paper according to claim 6, wherein the aramid fibrids comprise meta-aramid fibrids andor para-aramid fibrids.
8. The paper according to claim 1, wherein the length of the microfilaments is at least 3 mm.
9. The paper according to claim 1, wherein the microfilament titer is less than 1.2 dtex.
10. The paper according to claim 9, wherein the microfilament has a titer of at least at least 0.3 dtex.
11. The paper according to claim 1, wherein the microfilaments have an average diameter of 1 to 499 nm.
12. The paper according to claim 1, wherein the paper has a grammage of less than 60 gm2.
13. The paper according to claim 1, wherein the filaments have an aspect ratio of a least 4 mmdtex.
14. A fuel cell, a battery, a capacitor, a printed wiring board, a honeycomb, a packaging, a separator for electrical isolation, or a filter comprising the paper according to claim 1.
15. (canceled)
16. The paper according to claim 2, wherein the paper further comprises at least 20% of pulp as the non-resinous binder.
17. The paper according to claim 5, wherein the aramid microfilaments are para-aramid microfilaments.
18. The paper according to claim 1, wherein the length of the microfilaments is at least 4 mm.
19. The paper according to claim 1, wherein the length of the microfilaments is at most 15 mm.
20. The paper according to claim 1, wherein the length of the microfilaments is at most 8 mm.
21. The paper according to claim 1, wherein the microfilaments have an average diameter of particular 50-300 nm.