1460757745-b1547b62-c7a9-49ae-8ed2-09cccbbe8fa4

What is claimed is:

1. An enclosure for a wildlife surveillance system, the enclosure comprising:
a base configured to support at least a portion of the surveillance system;
a cover coupled to the base with a hinge so as to permit rotating movement therebetween; and
a foam insert disposed intermediate the surveillance system and an inside surface of said cover to minimize light reflected from the inside surface from interacting with the surveillance system.
2. The enclosure of claim 1, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure.
3. The enclosure of claim 1, wherein both said base and said cover include a first pair of holes and a second pair of holes that are aligned with one another when the cover is in a closed position.
4. The enclosure of claim 3, wherein the first pair of holes are configured to accommodate a lock to lock the cover to the base.
5. The enclosure of claim 3, wherein the second pair of holes are threaded to accommodate a fastener to clamp the cover to the base.
6. The enclosure of claim 1, wherein the base further comprises first and second compartments and wherein said first and second compartments are environmentally isolated from one another.
7. The enclosure of claim 1, wherein said hinge further includes a pin and a plurality of adjacent female portions, said adjacent female portions being adapted to receive said pin, and wherein at least a portion of said pin and at least some of said adjacent female portions are configured to interlock so as to selectively prevent rotating movement of said cover relative to said base.
8. The enclosure of claim 1, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure,
wherein said base includes first and second compartments environmentally isolated from one another, and wherein said locking assembly is coupled to said second compartment.
9. The enclosure of claim 1, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure,
wherein said locking assembly includes:
a flexible cable having opposed ends and having an abutment coupled to one of said ends;
a collar slidably coupled to said flexible cable and adapted to be locked to said flexible cable at a desired position; and
wherein said enclosure includes a plurality of openings through which said flexible cable is received.
10. The enclosure of claim 9, wherein said base includes first and second compartments environmentally isolated from one another, and wherein said plurality of openings are formed in one of said first and second compartments.
11. The enclosure of claim 1, wherein the surveillance system includes a camera with a flash, the flash being the source of the light.
12. An enclosure for a wildlife surveillance system, the enclosure comprising:
a base configured to support at least a portion of the surveillance system;
a cover coupled to the base with a hinge so as to permit rotating movement therebetween; and
a cover coupled to the base with a hinge so as to permit rotating movement therebetween; and
means for minimizing light generated by the surveillance system within the enclosure from interacting with the surveillance system.
13. The enclosure of claim 12, wherein the means for minimizing light generated by the surveillance system within the enclosure comprises a foam insert disposed intermediate the surveillance system and an inside surface of said cover to minimize light reflected from the inside surface from interacting with the surveillance system.
14. The enclosure of claim 12, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure.
15. The enclosure of claim 12, wherein the base further comprises first and second compartments and wherein said first and second compartments are environmentally isolated from one another.
16. The enclosure of claim 12, wherein said hinge further includes a pin and a plurality of adjacent female portions, said adjacent female portions being adapted to receive said pin, and wherein at least a portion of said pin and at least some of said adjacent female portions are configured to interlock so as to selectively prevent rotating movement of said cover relative to said base.
17. The enclosure of claim 12, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure,
wherein said base includes first and second compartments environmentally isolated from one another, and wherein said locking assembly is coupled to said second compartment.
18. The enclosure of claim 12, further comprising a locking assembly that secures the enclosure to a mounting structure, said locking assembly preventing removal of the enclosure from said mounting structure without opening said cover of the enclosure,
wherein said locking assembly includes:
a flexible cable having opposed ends and having an abutment coupled to one of said ends;
a collar slidably coupled to said flexible cable and adapted to be locked to said flexible cable at a desired position; and
wherein said enclosure includes a plurality of openings through which said flexible cable is received.
19. A method of minimizing light in a wildlife surveillance system, the method comprising the steps of:
providing an enclosure having a base and a cover;
disposing a foam insert intermediate the surveillance system and an inside surface of said cover; and
minimizes light reflected from the inside surface from interacting with the surveillance system.
20. The method of claim 18, wherein the base has first and second compartments environmentally isolated from one another, and wherein one of the first and second compartments includes a plurality of openings;
further comprising the steps of:
providing a locking assembly; and
securing the enclosure to a mounting structure by coupling the locking assembly to the openings such that the enclosure cannot be removed from the mounting structure without opening the cover.
21. The method of claim 19, wherein the locking assembly includes a flexible cable and a lock, and wherein the cover includes an opening adapted to align with one of the plurality of openings when the cover is closed on the base, and wherein said securing step includes mating the cable with others of the plurality of openings and locking the lock through the aligned openings of the cover and the base.
22. The method of claim 19, wherein the surveillance system includes a camera with a flash, the flash being the source of the light reflected from the inside surface.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

What is claimed is:

1. A method of internally calibrating a multiplexed assay comprising:
(a) exposing a mixture comprising a pooled population of at least one subset of particles to a reagent mixture comprising at least one type of signal ligand, wherein the particles within a subset are: (i) similarly-sized; (ii) exhibit at least one characteristic classification parameter that distinguish the particles of one subset from those of another; and (iii) comprise a tracer or a ligand binding partner specific to a target ligand, the ligand binding partner associated with one subset of particles may be the same as or different from the ligand binding partner associated with another subset of particles, and the target ligand is chosen from analytes suspected of being in a sample, tracers, and the at least one type of signal ligand, provided either that: (i) a specific target ligand is an analyte chosen from interfering factors and characteristic sample components excluding the analyte or analytes of interest, or (ii) the particles of at least one subset comprise a known concentration of a specific ligand binding partner corresponding to an analyte of interest, the concentration being chosen to test for a hook effect, or (iii) in one or more groups of subsets of particles, the particles in the subsets of the group comprise the same tracer or a ligand binding partner corresponding to same target ligand but at known concentrations that vary with each member of the one or more selected groups;
(b) passing the exposed particles through an examination zone; and
(c) collecting data as the exposed particles pass through the examination zone relating to: (i) one or more characteristic classification parameters of each particle including data on signal intensities, and at least either (ii) the presence or absence of a complex formed between the ligand binding partner and target ligand or (iii) the apparent concentrations of the one or more particular analytes of interest associated with each member of the one or more selected groups, or both (ii) and (iii).
2. A method according to claim 1, further comprising substantially simultaneously with collecting, classifying each particle according to its subset and, when the target ligand is an analyte, quantifying an amount of analyte associated with each subset.
3. A method according to claim 1, wherein the at least one subset of particles is at least two subsets of particles, the particles of a first subset comprise ligand binding partner specific for target ligand chosen from analytes and the particles of at least a second subset comprise ligand binding partner specific for target ligand chosen from signal ligand.
4. A method according to claim 3, wherein the at least one subset of particles is at least five subsets of particles, the particles of the second, third, fourth, and fifth subset of particles comprise ligand binding partner specific for the same signal ligand but at known concentrations that vary with each subset.
5. A method according to claim 1, wherein the at least one subset of particles is at least two subsets of particles, the particles of a first subset comprise ligand binding partner capable of binding a first or a second target ligand, wherein the first target ligand is a tracer and the second target ligand is an analyte, and the particles of at least a second subset comprise a tracer equivalent to the first target ligand.
6. A method according to claim 5, wherein the at least one subset of particles is at least five subsets of particles, the particles of the second, third, fourth, and fifth subset of particles comprise the same tracer but at known concentrations that vary with each subset.
7. A method according to claim 1, wherein the identity of a ligand binding partner is selected to provide information relating to the inclusion or omission of a sample or reagent in the reagent mixture.
8. A method according to claim 1, wherein the signal ligands and tracers comprise at least one fluorochrome and the signal intensities are fluorescence emission intensities.
9. A method according to claim 1, wherein the particles in each subset exhibit two or more characteristic fluorescence emission classification parameters.
10. A method according to claim 9, wherein particles of one subset differ from particles of another subset in an intensity of at least one fluorescence emission classification parameter.
11. A method according to claim 1, wherein one or more analytes is chosen from antigens, antibodies, peptides, proteins, nucleic acid sequences, and enzymes.
12. A method according to claim 2, wherein results of the method are displayed in real time.
13. A method according to claim 12, wherein the results take into account the known and apparent concentrations of the one or more particular analytes associated with each member of the one or more selected groups of subsets of particles.
14. A method according to claim 1, wherein the ligand binding partner is chosen from antibodies, antigens, synthetic oligonucleotides, and capture probes.
15. A method according to claim 1, wherein the identity or amount of ligand binding partner is selected to provide information relating to high-dose hook effects, interfering assay factors, sample or reagent omission, or dilutional linearity.

1460757737-2d87d0a4-464c-425c-a6f6-655294b9ecde

1. A liquid crystal display device, comprising: a liquid crystal panel and a first optical compensation structure and a second optical compensation structure located at two sides of the liquid crystal panel respectively, the first optical compensation structure comprises a first compensation film layer, a second compensation film layer and a first polarizing film layer located between the first compensation film layer and the second compensation film layer, wherein, the second optical compensation structure comprises: a polarizing film layer, a compensation film layer and a biaxial film layer, wherein,
the polarizing film layer is located between the compensation film layer and the biaxial film layer;
the biaxial film layer is located between the polarizing film layer and the liquid crystal panel;
wherein the second compensation film layer is located between the first polarizing film layer and the liquid crystal panel and
wherein the in-plane direction phase difference of the second compensation film layer and the thickness direction phase difference of the second compensation film layer are both 0 nm.
2. The liquid crystal display device according to claim 1, wherein, at least one of the first polarizing film layer and the polarizing film layer is a polyvinyl alcohol thin film; and at least one of the first compensation film layer, the second compensation film layer and the compensation film layer is a cellulose triacetate thin film.
3. The liquid crystal display device according to claim 1, wherein, the in-plane direction phase difference of the biaxial film layer is within the ranges from 200 nm to 300 nm; and
the thickness direction phase difference of the biaxial film layer is within the ranges from 100 nm to 150 nm.
4. The liquid crystal display device according to claim 3, wherein, the in-plane direction phase difference of the biaxial film layer is 274 nm, and the thickness direction phase difference of the biaxial film layer is 138 nm.
5. The liquid crystal display device according to claim 1, wherein, the in-plane direction phase difference of the biaxial film layer is 274 nm, and the thickness direction phase difference of the biaxial film layer is 138 nm.
6. A method for manufacturing a liquid crystal display device, comprising:
forming a first polarizing film layer between a first compensation film layer and a second compensation film layer arranged opposite to each other, to obtain a first optical compensation structure;
forming a polarizing film layer between a compensation film layer and a biaxial film layer arranged opposite to each other, to obtain a second optical compensation structure; and
disposing the first optical compensation structure and the second optical compensation structure at two sides of a liquid crystal panel respectively, so that the biaxial film layer is located between the polarizing film layer and the liquid crystal panel,
wherein the first optical compensation structure is disposed so that the second compensation film layer is located between the first polarizing film layer and the liquid crystal panel, and
wherein the in-plane direction phase difference of the second compensation film layer and the thickness direction phase difference of the second compensation film layer are both 0 nm.
7. The method according to claim 6, wherein, the in-plane direction phase difference of the biaxial film layer is within the ranges from 200 nm to 300 nm; and
the thickness direction phase difference of the biaxial film layer is within the ranges from 100 nm to 150 nm.
8. The method according to claim 7, wherein, the in-plane direction phase difference of the biaxial film layer is 274 nm, and the thickness direction phase difference of the biaxial film layer is 138 nm.
9. The method according to claim 6, wherein, at least one of the first polarizing film layer and the polarizing film layer is a polyvinyl alcohol thin film; and at least one of the first compensation film layer, the second compensation film layer and the compensation film layer is a cellulose triacetate thin film.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A computer-implemented method for analyzing a collision between a first vehicle and a second vehicle, comprising:
receiving information obtained from an event data recorder of said first vehicle that records black box information, at a computer system remote from the first vehicle and the second vehicle;
determining an impact severity of said first and second vehicles in the computer system using said information, the impact severity including a change in velocity for the first and second vehicles, by modeling the impact severity in the computer system using the Information; and
reporting the impact severity from the computer system.
2. The method of claim 1, wherein said information comprises a change in velocity of said first vehicle, and further comprising calibrating said change in velocity prior to using said information.
3. The method of claim 1, said receiving comprising receiving said information via a network of interconnected computers or via wireless transmission.
4. The method of claim 1, further comprising storing said information in a secure manner.
5. The method of claim 1, further comprising comparing said impact severity determined from said information to a second determination of impact severity, said second determination calculated in said computer system without said information.
6. The method of claim 1, wherein said information comprises a change in velocity and a pre-impact speed of said first vehicle.
7. The method of claim 1, further comprising using the information in the computer system in connection with a database of vehicle data including benchmark data for a benchmark vehicle related to one of the first or second vehicles to determine the impact severity of the one of the first or second vehicles.
8. The method of claim 1, further comprising using the information in the computer system in connection with repair estimate information for at least one of the first and second vehicles.
9. The method of claim 1, further comprising determining a point of impact and a principal direction of force for each of the first and second vehicles.
10. The method of claim 5, further comprising weighting said impact severity determined from said information differently than the second determination of impact severity.
11. The method of claim 6, wherein using said information comprises determining a change in velocity of said second vehicle based on said change in velocity and said pre-impact speed of said first vehicle.
12. The method of claim 6, wherein using said information further comprises determining a relative velocity of said first and second vehicles based on said change in velocity of said first vehicle.
13. The method of claim 9, further comprising determining the impact severity of at least one of the first and second vehicles using the determined point of impact and the determined principal direction of force.
14. The method of claim 11, further comprising adjusting said change in velocity of said first vehicle prior to using said information.
15. A computer-implemented method for determining impact severity of a collision between a first vehicle and a second vehicle, comprising:
receiving information obtained from an event data recorder of said first vehicle that records black box data, during a claim evaluation process;
electronically storing said information on a storage medium, said information comprising the black box data;
electronically accessing said information from said storage medium;
selecting one of different algorithms to use in modeling said impact severity based on a type of said event data recorder, wherein the selection is further based on a type or characteristics of information provided by the type of event data recorder;
utilizing said information in a computer to model said impact severity via the selected algorithm; and
reporting the impact severity from the computer.
16. The method of claim 15, wherein said information comprises a change in velocity of said first vehicle; and wherein said modeling is based at least in part on said change in velocity.
17. The method of claim 15, further comprising performing a simulation to generate a distribution of change in velocity calculations for said first vehicle using said information.
18. The method of claim 17, further comprising determining if said distribution is consistent with at least one user input.
19. A computer-implemented method for assessing an accident involving a first vehicle, comprising:
receiving black box information obtained from an event data recorder of said first vehicle;
extracting non-visual data from said black box information;
determining a first measure of impact severity by modeling the impact severity using said non-visual data in a system external to the first vehicle;
determining a second measure of impact severity in the system, the second measure of impact severity determined from information obtained from a source independent of the first vehicle;
weighting differently in the system the first measure of impact severity and the second measure of impact severity to develop a final measure of impact severity; and
storing the final measure of impact severity in the system for later use.
20. The method of claim 19, wherein said information comprises a change in velocity of said first vehicle.
21. The method of claim 19, further comprising selecting an algorithm to use in determining said first measure of impact severity based on a type of said event data recorder.
22. The method of claim 19, further comprising:
receiving input information related to the accident from a user of the system; and
determining if at least one inconsistency exists between the input information and the first measure of impact severity or the second measure of impact severity.
23. The method of claim 20, further comprising adjusting said change in velocity before determining said first measure of impact severity.
24. The method of claim 23, further comprising performing a simulation to generate a distribution of change in velocity calculations for said first vehicle using said adjusted change in velocity.
25. A computer-implemented method for analyzing a vehicle accident, comprising:
receiving information harvested from an event data recorder of a first vehicle in a system external to the first vehicle;
storing said information in a secure manner in said system;
accessing said information in said system;
determining a first measure of impact severity for said first vehicle in said system by modeling based at least in part on said information harvested from the event data recorder;
determining a second measure of impact severity in said system for said first vehicle without said information and using information obtained from an independent source from the event data recorder; and
storing the first measure of impact severity and the second measure of impact severity for later use.
26. The method of claim 25, wherein said information comprises a change in velocity of said first vehicle.
27. The method of claim 25, further comprising weighting the first measure of impact severity and the second measure of impact severity differently.
28. The method of claim 25, further comprising determining the second measure of impact severity for the first vehicle using repair estimate information.
29. The method of claim 25, further comprising determining the second measure of impact severity for the first vehicle using crush information.
30. The method of claim 26, further comprising adjusting said change in velocity before determining said first measure of impact severity.
31. The method of claim 26, further comprising determining a pre-impact speed reduction of said first vehicle based at least in part on said information.
32. The method of claim 31, further comprising using the reduced pre-impact speed in determining said first measure of impact severity.