1. A homogeneous immunoassay method of testing for an allergic response in a patient, comprising:
combining an undiluted serum sample from the patient with labeled subsets of specific allergen-coupled particles under conditions that enable binding of the allergen-coupled particles to allergen-specific antibodies from the patient’s serum sample, wherein the combining creates a response mixture;
adding a first binding agent to the response mixture, the first binding agent comprising an anti-human antibody covalently bound to a first member of a binding pair;
adding a labeled second binding agent to the response mixture containing the first binding agent, the labeled second binding agent comprising a second member of the binding pair and a label, wherein the second member of the binding pair has an affinity for binding to the first member of the binding pair;
determining the amounts of the allergen-specific antibodies from the serum sample that bound to specific allergens by (i) identifying and distinguishing the labeled particles to identify the subsets of specific allergens and (ii) measuring the amount of labeled second binding agent in each of the subsets of allergens;
wherein, the first binding agent and the second binding agent are preselected to enable detection of anti-human antibodies at a detection sensitivity ranging from about 10\u22129 gml to about 10\u221212 gml, and the undiluted serum is limited to having a volume of about 0.5 \u03bcL to about 25 \u03bcL;
wherein, the molecular ratio of anti-human antibody to the first member of the binding pair in the first binding agent is from about 1:10 to about 1:30, the second binding agent has a molecular weight of between about 400,000 and about 1,000,000 Daltons, and the selection of the first binding agent, the second binding agent, and serum volume has the function of avoiding quenching of a signal from the label of the labeled second binding agent and high dose hook effects;
and wherein, the detection sensitivity ranging from 10\u22129 gml to 10\u221212 gml is measured under homogeneous assay conditions.
2. The method of claim 1, wherein the labeled particles are fluorescently labeled microspheres, and the undiluted serum sample ranges in volume from about 1.0 \u03bcL to about 10 \u03bcL.
3. The method of claim 1, wherein the first binding agent comprises an anti-human antibody-biotin conjugate having about 15-25 biotin molecules per antibody molecule, the second binding agent comprises a streptavidin-phycoerytherin conjugate, and the mole ratio of the first binding agent to the second binding agent ranges from about 1:1 to about 1:5.
4. The method of claim 1, wherein the labeled particles enable identification of subsets of allergens, and the subsets compose a panel of allergens having from about 2 specific allergens to about 100 specific allergens.
5. The method of claim 1, wherein the method further comprises providing the patient with a kit having a device for puncturing the skin and obtaining a blood sample, and a container for holding and transmitting the blood sample to a laboratory for extracting the serum sample and measuring the allergic response of the patient to an allergen or panel of allergens.
6. The method of claim 1, wherein the method further comprises providing the patient with access to a computer network to obtain testing results.
7. The method of claim 1, wherein the first binding agent comprises an anti-human antibody-digoxin conjugate.
8. The method of claim 1, wherein the allergen-specific antibodies from the patient’s serum sample are allergen-specific IgG antibodies, and the anti-human antibody in the first binding agent is an anti-human IgG antibody.
9. A homogeneous immunoassay method of testing for an allergic response in a patient, comprising:
combining an undiluted serum sample from the patient with labeled subsets of specific allergen-coupled particles under conditions that enable binding of the allergen-coupled particles to allergen-specific IgG antibodies from the patient’s serum sample, wherein the combining creates a response mixture;
adding a first binding agent to the response mixture, the first binding agent comprising an anti-human IgG antibody covalently bound to a first member of a binding pair;
adding a labeled second binding agent to the response mixture containing the first binding agent, the labeled second binding agent comprising a second member of the binding pair and a label, wherein the second member of the binding pair has an affinity for binding to the first member of the binding pair;
determining the amounts of the allergen-specific antibodies from the serum sample that bound to specific allergens by (i) identifying and distinguishing the labeled particles to identify the subsets of specific allergens and (ii) measuring the amount of labeled second binding agent in each of the subsets of allergens;
wherein, the first binding agent and the second binding agent are preselected to enable detection of anti-human IgG antibodies at a detection sensitivity ranging from about 10\u22129 gml to about 10\u221212 gml, and the undiluted serum is limited to having a volume of about 0.5 \u03bcL to about 25 \u03bcL;
wherein, the molecular ratio of anti-human antibody to the first member of the binding pair in the first binding agent is from about 1:10 to about 1:30, the second binding agent has a molecular weight of between about 400,000 and about 1,000,000 Daltons, and the selection of the first binding agent, the second binding agent, and serum volume has the function of avoiding quenching of a signal from the label of the labeled second binding agent and high dose hook effects;
and wherein, the detection sensitivity ranging from 10\u22129 gml to 10\u221212 gml is measured under homogeneous assay conditions.
10. The method of claim 9, wherein the labeled particles are fluorescently labeled microspheres, and the undiluted serum sample ranges in volume from about 1.0 \u03bcL to about 10 \u03bcL.
11. The method of claim 9, wherein the first binding agent comprises an anti-human IgG-antibody-biotin conjugate having about 15-25 biotin molecules per antibody molecule, the second binding agent comprises a streptavidin-phycoerytherin conjugate, and the mole ratio of the first binding agent to the second binding agent ranges from about 1:1 to about 1:5.
12. The method of claim 9, wherein the labeled particles enable identification of subsets of allergens, and the subsets compose a panel of allergens having from about 2 specific allergens to about 100 specific allergens.
13. The method of claim 9, wherein the method further comprises providing the patient with a kit having a device for puncturing the skin and obtaining a blood sample, and a container for holding and transmitting the blood sample to a laboratory for extracting the serum sample and measuring the allergic response of the patient to an allergen or panel of allergens.
14. The method of claim 9, wherein the method further comprises providing the patient with access to a computer network to obtain testing results.
15. The method of claim 9, wherein the first binding agent comprises an anti-human IgG-antibody-digoxin conjugate.
16. The method of claim 9, wherein the allergen-specific antibodies from the patient’s serum sample are allergen-specific IgG antibodies, and the anti-human antibody in the first binding agent is an anti-human IgG antibody.
17. A method for a user to obtain a homogeneous immunoassay of an allergic response in a patient, comprising:
collecting a blood sample from a patient;
submitting the blood sample for analysis, the analysis comprising
extracting an undiluted serum sample from the blood sample, wherein the undiluted serum sample ranges in size from about 0.5 \u03bcL to about 25 \u03bcL;
combining the undiluted serum sample from the patient with labeled subsets of specific allergen-coupled particles under conditions that enable binding of the allergen-coupled particles to allergen-specific antibodies from the patient’s serum sample, wherein the combining creates a response mixture;
adding a first binding agent to the response mixture, the first binding agent comprising an anti-human antibody covalently bound to a first member of a binding pair;
adding a labeled second binding agent to the response mixture containing the first binding agent, the labeled second binding agent comprising a second member of the binding pair and a label, wherein the second member of the binding pair has an affinity for binding to the first member of the binding pair;
determining the amounts of the allergen-specific antibodies from the serum sample that bound to specific allergens by (i) identifying and distinguishing the labeled particles to identify the subsets of specific allergens and (ii) measuring the amount of labeled second binding agent in each of the subsets of allergens;
wherein,
the first binding agent and the second binding agent are preselected to enable detection of anti-human antibodies at a detection sensitivity ranging from about 10\u22129 gml to about 10\u221212 gml, and the undiluted serum is limited to having a volume of about 0.5 \u03bcL to about 25 \u03bcL;
the molecular ratio of anti-human antibody to the first member of the binding pair in the first binding agent is from about 1:10 to about 1:30, the second binding agent has a molecular weight of between about 400,000 and about 1,000,000 Daltons, and the selection of the first binding agent, the second binding agent, and serum volume has the function of avoiding quenching of a signal from the label of the labeled second binding agent and high dose hook effects; and,
the detection sensitivity ranging from 10\u22129 gml to 10\u221212 gml is measured under homogeneous assay conditions;
and,
receiving results of the homogeneous immunoassay of the allergic response in the patient.
18. The method of claim 17, wherein the user is the patient.
19. The method of claim 17, wherein the user is a physician.
20. The method of claim 17, wherein the collecting includes using a kit having a device for puncturing the skin of the patient and obtaining the blood sample, and a container for holding and transmitting the blood sample to a laboratory for extracting the serum sample and measuring the allergic response of the patient to an allergen or panel of allergens.
21. The method of claim 17, wherein the receiving includes using a computer network having test results for the patient on a non-transitory computer readable storage medium accessible to obtain the testing results.
22. The method of claim 17, wherein the allergen-specific antibodies from the patient’s serum sample are allergen-specific IgE antibodies, and the anti-human antibody in the first binding agent is an anti-human IgE antibody.
23. The method of claim 17, wherein the allergen-specific antibodies from the patient’s serum sample are allergen-specific IgG antibodies, and the anti-human antibody in the first binding agent is an anti-human IgG antibody.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A hybrid cotton plant designated INTERCOTT-35, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4831.
2. Seed of said hybrid cotton plant of claim 1.
3. A tissue culture of regenerable cells of said hybrid cotton plant of claim 1.
4. The tissue culture of claim 3, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
5. A tissue culture of claim 4, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
6. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-35, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, an average period of 140 days to 50 percent open boll, a fiber length average not lower than 1.34 inches, a filer strength average not lower than 36 gram per tex, a fiber fineness average not higher than 3.9 micronaire, tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
7. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-195, and Gossypium barbadense designated line R-208 as a source of breeding material.
8. The method of claim 7, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
9. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-195 and R-208 or parts of said cotton plants as a source of the breeding material.
10. The system of claim 9, wherein at least one of said cotton plants is male sterile.
11. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle period shorter than 170 days, and infestation of pathogens.
12. The cotton plant of claim 11, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., and Alternaria spp.
13. The cotton plant of claim 11, wherein the cotton plant is hybrid cotton plant INTERCOTT-35 and its parents are cotton plants A-195 and R-208.
14. A planted field comprising cotton plants A-195 and R-208.
15. The planted field of claim 14, wherein at least one of said cotton plants is male sterile.
16. The planted field of claim 14, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-195 and R-208.
17. A planted field comprising cotton plants B-195 and R-208.
18. A hybrid cotton plant designated INTERCOTT-51, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4833.
19. Seed of said hybrid cotton plant of claim 18.
20. A tissue culture of regenerable cells of said hybrid cotton plant of claim 18.
21. The tissue culture of claim 20, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
22. A tissue culture of claim 21, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
23. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-5 1, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, an average period of 130 days to 50 percent open boll, a fiber length average not lower than 1.33 inches, a fiber strength average not lower than 34 gram per tex, a fiber fineness average not higher than 3.9 micronaire. tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
24. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-151, and Gossypium barbadense designated line R-208 as a source of breeding material.
25. The method of claim 24, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
26. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-151 and R-208 or parts of said cotton plants as a source of the breeding material.
27. The system of claim 26, wherein at least one of said cotton plants is male sterile.
28. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle period shorter than 160 days, and infestation of pathogens.
29. The cotton plant of claim 28, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., and Alternaria spp.
30. The cotton plant of claim 28, wherein the cotton plant is hybrid cotton plant INTERCOTT-51 and its parents are cotton plants A-151 and R-208.
31. A planted field comprising cotton plants A-151 and R-208.
32. The planted field of claim 31, wherein at least one of said cotton plants is male sterile.
33. The planted field of claim 31, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-151 and R-208.
34. A planted field comprising cotton plants B-151 and R-208.
35. A hybrid cotton plant designated INTERCOTT-75, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4834.
36. Seed of said hybrid cotton plant of claim 35.
37. A tissue culture of regenerable cells of said hybrid cotton plant of claim 35.
38. The tissue culture of claim 37, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
39. A tissue culture of claim 38, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
40. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-75, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, an average period of 120 days to 50 percent open boll, a fiber length average not lower than 1.32 inches, a fiber strength average not lower than 33 gram per tex, a fiber fineness average not higher than 3.9 micronaire, tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
41. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-175 ATCC, and Gossypium barbadense designated line R-208 as a source of breeding material.
42. The method of claim 41, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
43. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-175 and R-208 or parts of said cotton plants as a source of the breeding material.
44. The system of claim 43, wherein at least one of said cotton plants is male sterile.
45. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle period shorter than 150 days, and infestation of pathogens.
46. The cotton plant of claim 45, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., and Alternaria spp.
47. The cotton plant of claim 45, wherein the cotton plant is hybrid cotton plant INTERCOTT-75 and its parents are cotton plants A-175 and R-208.
48. A planted field comprising cotton plants A-175 and R-208.
49. The planted field of claim 48, wherein at least one of said cotton plants is male sterile.
50. The planted field of claim 48, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-175 and R-208.
51. A planted field comprising cotton plants B-175 and R-208.
52. A hybrid cotton plant designated INTERCOTT-34, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4831.
53. Seed of said hybrid cotton plant of claim 52.
54. A tissue culture of regenerable cells of said hybrid cotton plant of claim 52.
55. The tissue culture of claim 54, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
56. A tissue culture of claim 55, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
57. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-34, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, an average period of 125 days to 50 percent open boll, a fiber length average not lower than 1.33 inches, a fiber strength average not lower than 36 gram per tex, a fiber fineness average not higher than 4.0 micronaire, tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
58. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-34, and Gossypium barbadense designated line R-208 as a source of breeding material.
59. The method of claim 58, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
60. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-34 and R-208 or parts of said cotton plants as a source of the breeding material.
61. The system of claim 60, wherein at least one of said cotton plants is male sterile.
62. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle period shorter than 160 days, and infestation of pathogens.
63. The cotton plant of claim 62, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., and Alternaria spp.
64. The cotton plant of claim 62, wherein the cotton plant is hybrid cotton plant INTERCOTT-34 and its parents are cotton plants A-34 and R-208.
65. A planted field comprising cotton plants A-34 and R-208.
66. The planted field of claim 65, wherein at least one of said cotton plants is male sterile.
67. The planted field of claim 65, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-34 and R-208.
68. A planted field comprising cotton plants B-34 and R-208.
69. A hybrid cotton plant designated INTERCOTT-145, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4836.
70. Seed of said hybrid cotton plant of claim 69.
71. A tissue culture of regenerable cells of said hybrid cotton plant of claim 69.
72. The tissue culture of claim 71, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
73. A tissue culture of claim 72, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
74. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-145, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, tolerant to Cicadellidae insects, an average period of 130 days to 50 percent open boll, a fiber length average not lower than 1.34 inches, a fiber strength average not lower than 35 gram per tex, a fiber fineness average not higher than 3.9 micronaire, tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
75. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-14, and Gossypium barbadense designated line R-205 as a source of breeding material.
76. The method of claim 75, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
77. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-14 and R-205 or parts of said cotton plants as a source of the breeding material.
78. The system of claim 77, wherein at least one of said cotton plants is male sterile.
79. The system of claim 77, wherein at least one of said cotton plants exhibits a hair cover on leaves and stems of a density higher than that of an Acala type cotton plant.
80. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle, period shorter than 160 days, and infestation of pathogens.
81. The cotton plant of claim 80, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., Alternaria spp and Cicadellidae spp.
82. The cotton plant of claim 80, wherein the cotton plant is hybrid cotton plant INTERCOTT-145 and its parents are cotton plants A-14 and R-205.
83. A planted field comprising cotton plants A-14 and R-205.
84. The planted field of claim 83, wherein at least one of said cotton plants is male sterile.
85. The planted field of claim 83, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-14 and R-205.
86. A planted field comprising cotton plants B-14 and R-205.
87. A hybrid cotton plant designated INTERCOTT-83, representative seed of said hybrid cotton plant having been deposited under ATCC Patent Depository No.: PTA-4835.
88. Seed of said hybrid cotton plant of claim 87.
89. A tissue culture of regenerable cells of said hybrid cotton plant of claim 87.
90. The tissue culture of claim 89, wherein the tissue culture regenerates plants capable of expressing all the morphological and physiological characteristics of said hybrid cotton plant.
91. A tissue culture of claim 90, wherein said tissue culture is regenerated from cells or protoplasts of a tissue selected from the group consisting of seeds, leaves, stems, pollens, roots, root tips, anthers, ovules, petals, flowers, embryos, fibers and bolls.
92. A cotton plant, or its parts, wherein at least one ancestor of said hybrid cotton plant is the cotton plant INTERCOTT-83, said cotton plant capable of expressing at least one trait selected from the group consisting of a tolerance to suboptimal water supply, a tolerance to salinity, a tolerance to suboptimal temperature, a tolerance to suboptimal light, a resistance to Fusarium wilt, a resistance to Verticillium wilt, a resistance to Alternaria leaf spot, an average period of 125 days to 50 percent open boll, a fiber length average not lower than 1.33 inches, a fiber strength average not lower than 34 gram per tex, a fiber fineness average not higher than 3.6 micronaire, tipped oval shaped boll, an average number of 4-5 locules per boll and light yellow petals.
93. A method of developing a hybrid cotton plant using plant breeding techniques which employ a cotton plant, or its parts, as a source of plant breeding material, the method comprising utilizing cotton plants Gossypium hirsutum designated line A-83, and Gossypium barbadense designated line R-208 as a source of breeding material.
94. The method of claim 93, wherein the plant breeding techniques are selected from the group consisting of recurrent selection, backcrossing, pedigree breeding, restriction fragment length polymorphism enhanced selection, genetic marker enhanced selection, and transformation.
95. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising cotton plants A-83 and R-208 or parts of said cotton plants as a source of the breeding material.
96. The system of claim 95, wherein at least one of said cotton plants is male sterile.
97. A cotton plant characterized by a combination of traits leading to a commercial yield higher than at least one parent of the cotton plant under growth conditions, selected from the group consisting of suboptimal water supply, suboptimal salinity, suboptimal temperature, suboptimal light, a growth cycle period shorter than 160 days, and infestation of pathogens.
98. The cotton plant of claim 97, further characterized by a naked Pima type seed coat.
99. The cotton plant of claim 97, wherein said pathogens are selected from the group consisting of Verticillium spp., Fusarium spp., and Alternaria spp.
100. The cotton plant of claim 97, wherein the cotton plant is hybrid cotton plant INTERCOTT-83 and its parents are cotton plants A-83 and R-208.
101. A planted field comprising cotton plants A-83 and R-208.
102. The planted field of claim 101, wherein at least one of said cotton plants is male sterile.
103. The planted field of claim 101, wherein the field is characterized by a planting pattern enabling cross pollination between cotton plants A-83 and R-208.
104. A planted field comprising cotton plants B-83 and R-208.
105. A method of developing a hybrid cotton plant using plant breeding techniques, the method comprising utilizing a first cotton plant selected from the group consisting of A-195, A-151, A-175, A-34, A-14 and A-83, and a second cotton plant selected from the group consisting of R-205 and R-208, as sources of breeding material.
106. A system for developing a hybrid cotton plant using plant breeding techniques, the system comprising utilizing a first cotton plant selected from the group consisting of A-195, A-151, A-175, A-34, A-14 and A-83, and a second cotton plant selected from the group consisting of R-205 and R-208, as sources of breeding material.