Author: claimsbot

1. A domain gateway control system, comprising:
a domain, wherein a first domain packet is transmitted in the domain, and a domain type code data corresponding to the domain is recorded in an Ethernet-type field of the first domain packet; and
a gateway device, configured at an interface of the domain and linked to an Ethernet, wherein the gateway device is used to acquire a first data packet from the Ethernet and analyze a destination MAC (media access control) address data of the first data packet;
when the gateway device determines that the destination MAC address data of the first data packet points to the domain, the gateway device converts the first data packet into a first domain packet by using a MAC Address Group Protocol information, and forwards the first domain packet to the domain; and the MAC Address Group Protocol information comprises: a recorded gateway MAC address data of the gateway device in a source MAC field of the first data packet, and the recorded domain type code data in an Ethernet-type field of the first data packet.
2. The domain gateway control system according to claim 1, wherein the gateway device comprises a gateway MAC table data, the gateway device learns a source MAC address data of the first data packet and records a communication port number data of communication port receiving the source MAC address data in the gateway MAC table data.
3. The domain gateway control system according to claim 1, wherein the gateway device is used to acquire a second domain packet transmitted in the domain, and the gateway device analyzes a destination MAC address data of the second domain packet to decide whether to forward the second domain packet.
4. The domain gateway control system according to claim 3, wherein the gateway device receives the second domain packet when the gateway device determines that the destination MAC address data of the second domain packet points to the gateway device, and a data field of the second domain packet does not include an off-net destination MAC address data.
5. The domain gateway control system according to claim 3, wherein when the destination MAC address data of the second domain packet points to the gateway device and a data field of the second domain packet comprises an off-net destination MAC address data directed to the Ethernet and an Ethernet-type code data corresponding to the Ethernet, the gateway device updates a destination MAC address data field of the second domain packet with the off-net destination MAC address data and updates an Ethernet-type field of the second domain packet with the Ethernet-type code data, to convert the second domain packet into a second data packet and forward the second data packet to the Ethernet.
6. The domain gateway control system according to claim 1, wherein any net device is configured in the domain and the net device comprises a device MAC table data, and the net device acquires a first domain packet and makes a pair of the source MAC address data of the first domain packet and the communication port number data of the communication port receiving the first domain packet, and records the pair in a device MAC table data of the net device.
7. The domain gateway control system according to claim 1, wherein the MAC Address Group Protocol information further comprises a recorded Ethernet-type code data corresponding to the Ethernet and an original source MAC address data in the data field of the first data packet, and the original source MAC address data is a MAC of an external device configured in the Ethernet.
8. The domain gateway control system according to claim 7, wherein any net device configured in the domain comprises a cache MAC table data, the net device is used to acquire the first domain packet, make a pair of the original source MAC address data and communication port number data of a communication port receiving the first domain packet, and record the pair in the cache MAC table data.
9. The domain gateway control system according to claim 8, wherein the net device generates a data packet to be sent out according to the cache MAC table data, records the original source MAC address data in a destination MAC address data of the data packet to be sent out, and outputs the data packet to be sent out through the communication port corresponding to the original source MAC address data.
10. A domain gateway control method, applied in a gateway device, wherein the gateway device is configured at an interface of a domain and is linked to an Ethernet, and the domain gateway control method comprises:
acquiring a first data packet from the Ethernet; and
analyzing a destination MAC (media access control) address data of the first data packet through the gateway device; and
when the gateway device determine that the destination MAC address data of the first data packet points to the domain, using a MAC Address Group Protocol information to convert the first data packet into a first domain packet, forwarding the first domain packet to the domain, wherein the MAC Address Group Protocol information comprises: a recorded gateway MAC address data of the gateway device in a source MAC field of the first data packet, and a recorded domain type code data corresponding to the domain in an Ethernet-type field of the first data packet.
11. The domain gateway control method according to claim 10, wherein:
when a gateway device determines that the destination MAC address data of the first data packet points to a second Ethernet, the gateway device uses an MAGP information to convert the first data packet into the first domain packet, and forwards the first domain packet to the second Ethernet.
12. The domain gateway control method according to claim 10, wherein:
when the gateway device determines that the destination MAC address data of the first data packet points to a second Ethernet, the gateway device forwards the first data packet to the second Ethernet.
13. The domain gateway control method according to claim 10, wherein:
when the gateway device determines that the destination MAC address data of the first data packet points to the gateway device, the gateway device receives the first data packet.
14. The domain gateway control method according to claim 10, further comprising:
acquiring a second domain packet from the domain; and
analyzing the second domain packet to decide whether to forward the second domain packet.
15. The domain gateway control method according to claim 14, wherein:
when the gateway device determines that the destination MAC address data of the second domain packet points to the gateway device, and the data field of the second domain packet includes an off-net destination MAC address data that points to a first Ethernet and an Ethernet-type code data corresponds to the first Ethernet, the gateway device updates the destination MAC address data field of the second domain packet with the off-net destination MAC address data and updates an Ethernet-type field of the second domain packet with the Ethernet-type code data to convert the second domain packet into a second data packet and forward the second data packet to the first Ethernet.
16. The domain gateway control method according to claim 10, further comprising:
acquiring, by any net device, the first domain packet, wherein the net device is configured in the domain; and
making a pair, by the net device, of a source MAC address data of the first domain packet and communication port number data of a communication port receiving the first domain packet, and recording the pair in a device MAC table data of the net device.
17. The domain gateway control method according to claim 16, further comprising:
analyzing the destination MAC address data of the first domain packet by any net device;
receiving the first domain packet by the net device when determining that the destination MAC address data of the first domain packet points to the net device; and
forwarding the first domain packet by the net device when determining that the destination MAC address data of the first domain packet is not directed to the net device, the net device.
18. The domain gateway control method according to claim 10, wherein the MAC Address Group Protocol information further comprises a recorded Ethernet-type code data corresponding to the Ethernet and an original source MAC address data in a data field of the first data packet, and the original source MAC address data is a MAC of an external device configured in the Ethernet.
19. The domain gateway control method according to claim 18, further comprising:
acquiring, by any net device, the first domain packet, wherein the net device is configured in the domain; and
making a pair, by the net device, of an original source MAC address data and communication port number data of a communication port receiving the first domain packet, and recording the pair in a cache MAC table data of the net device.
20. The domain gateway control method according to claim 19, further comprising:
generating a data packet to be sent out by any net device;
reading a cache MAC table data to acquire an original source MAC address data and a communication port number data corresponding to the original source MAC address data by the net device;
recording the original source MAC address data in a destination MAC address data of the data packet to be sent out by the net device; and
outputting the data packet to be sent out by the net device.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a cynomolgus monkey Dickkopf-4 (cDkk-4) protein which has an amino acid sequence as set forth in SEQ ID NO:2.
2. The isolated nucleic acid of claim 1 wherein the nucleic acid is a DNA.
3. The isolated nucleic acid of claim 1 wherein the nucleic acid is an RNA.
4. The isolated nucleic acid of claim 1 wherein the nucleic acid is a cDNA.
5. The isolated nucleic acid of claim 1 wherein the nucleic acid has a nucleotide sequence as set forth in SEQ ID NO:1.
6. An isolated protein comprising an amino acid sequence as set forth in SEQ ID NO:2.
7-9. (canceled)
10. A method for producing a cynomolgus monkey Dickkopf-4 (cDkk-4) protein which binds a low-density lipoprotein receptor protein 5 (LRP5) comprising:
(a) providing a nucleic acid encoding the cDkk-4 protein operably linked to a heterologous promoter;
(b) introducing the nucleic acid into a cell to produce a recombinant cell; and
(c) culturing the recombinant cell under conditions which allows expression of the cDkk-4 protein to produce the cDkk-4.
11. A method for determining whether an analyte is an antagonist of Dickkopf 4 (Dkk-4) comprising:
(a) providing a polypeptide comprising the extracellular domain of a Dkk-4 receptor;
(b) contacting the polypeptide with a cynomolgus monkey Dkk-4 (cDkk-4) and the analyte; and
(c) determining whether binding of the cDkk-4 to the polypeptide is decreased in the presence of the analyte, wherein a decrease in the binding indicates that the analyte is an cDkk-4 antagonist.
12. The method of claim 11, wherein the Dkk-4 receptor is low-density lipoprotein receptor related protein 5 (LRP5) or low density lipoprotein receptor related protein 6 (LRP6).
13. The method of claim 11, wherein the Dkk-4 receptor is kremen1 or kremen2.
14. The method of claim 11 wherein the cDkk-4 is labeled.
15. The method of claim 11 wherein the cDkk-4 is a fusion protein.
16-37. (canceled)

1. A method for the synthesis of 18F-labeled trifluoromethylketones comprising the steps of
reacting 18F-F2 with a silyl ether compound having the general formula 1
wherein R refers to an alkyl group having between 1 and 24 carbon atoms or an aryl group having between 6 and 24 carbon atoms under reaction conditions sufficient to form a 18F-labeled trifluoromethylketone.
2. The method of claim 1, wherein the alkyl or the aryl group comprises a ring.
3. The method of claim 1, wherein the alkyl group is substituted with at least one halogen, nitro, or alkoxy group.
4. The method of claim 3, wherein the alkoxy group has one to eight carbon atoms.
5. The method of claim 3, wherein the substituent does not participate in the reaction.
6. The method of claim 3, wherein the alkoxy is substituted with at least one substituents selected from the group consisting of an alkyl group having between 1 and 8 carbon atoms, a halogen, and an amino group, or any combination thereof.
7. The method of claim 1, wherein the condition sufficient to form a 18F-labeled trifluoromethylketone include a reaction temperature of between about \u221250\xb0 C. to about \u221215\xb0 C.
8. The method of claim 1, wherein the 1819F-F2 is prepared by bombardment with 18OO2 in a cyclotron and mixing with non-radioactive F2.
9. The method of claim 1, wherein the 18F-F2 mixture is bubbled into a solution comprising silyl ether compounds for about 5 to 15 minutes.
10. The method of claim 1, wherein the silyl ether is 2,2-difluoroenol silyl ether and further wherein the 2,2-difluroenol silyl ether is prepared by:
mixing magnesium, tetrahydrofuran, and chlorotrimethylsilane to form a reactant mixture;
cooling the mixture to between about \u221215\xb0 C. to 5\xb0 C.;
adding trifluoroacetophenone to the cooled mixture; and
stirring the mixture for about 0.5 to 1.5 hours to produce the difluoroenol silyl ether.
11. The method of claim 10, wherein the difluroenol silyl ether is 2,2-difluoro-1-phenyl-1-trimethylsiloxy-ethene.
12. The method of claim 1, which further comprises:
dissolving the silyl ether compound in acetonitrile to form a solution;
cooling the solution to about \u221250\xb0 to about \u221215\xb0 C.;
preparing a mixture of 1819F-F2 and nitrogen; and
bubbling the mixture of 1819F-F2 and nitrogen into the solution for about 5 to 15 minutes to form a reaction mixture.
13. The method of claim 1, wherein the 18F-labeled trifluoromethylketones synthesized have a radiochemical purity greater than 99%.
14. The method of claim 1, wherein the 18F-labeled trifluoromethylketones that are synthesized have specific activities between about 15 to 20 GBqmmol at the end of synthesis.
15. The method of claim 1, wherein the radiochemical yields of the 18F-labeled trifluoromethylketones are between about 45 to 55%.
16. The method of claim 1, wherein the 18F-labeled trifluoromethylketones synthesized has the following general formula 2a.
17. The method of claim 1, wherein the 18F-labeled trifluoromethylketones synthesized has the following general formula 2b.
18. The method of claim 1, wherein the 18F-labeled trifluoromethylketones synthesized has the following general formula 2c.
19. The method of claim 1, wherein the 18F-labeled trifluoromethylketones synthesized has the following general formula 2d.
20. An imaging agent comprising the 18F-labeled trifluoromethyl ketone of claim 1.
21. The imaging agent of claim 20, having a radiochemical purity of about 99% for use in positron emission tomography.
22. A marker for detecting cell proliferation or viral infections comprising the 18F-labeled trifluoromethyl ketone of claim 1.
23. The marker of claim 22, having a radiochemical purity of about 99%.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of manufacturing polymer-coated particles useful for chemical mechanical polishing magnetic, optical, semiconductor or silicon substrates comprising:
providing a dispersion of particle cores in a non-aqueous solvent;
reacting a polymeric precursor within the dispersion to polymerize the polymeric precursor to form a polymer and to coat the particle cores with the polymer, the polymeric precursor being monomeric or oligomeric and the polymer coating at least a portion of the surface of the particle cores with the polymer and forming the polymer-coated particles having a solid outer polymeric shell;
substituting the non-aqueous solvent with water to form an aqueous mixture containing the polymer-coated particles; and
forming an aqueous chemical mechanical polishing formulation with the polymer-coated particles without drying the polymer-coated particles.
2. The method of claim 1 including the additional step of separating the polymer-coated particles as a supernatant.
3. The method of claim 1 wherein the aqueous substituting step relies upon azeotropic distilling to remove the non-aqueous solvent from the polymer-coated particles.
4. The method of claim 1 including the additional step of attaching functional groups to the polymer-coated particles.
5. The method of claim 1 wherein the dispersing particles in the non-aqueous solvent occurs without an earlier solvent transfer from an aqueous solution.
6. A method of manufacturing polymer-coated particles useful for chemical mechanical polishing magnetic, optical, semiconductor or silicon substrates comprising:
providing a dispersion of particle cores in a non-aqueous solvent;
reacting a polymeric precursor within the dispersion to polymerize the polymeric precursor to form a polymer and to coat the particle cores with the polymer, the polymeric precursor being monomeric or oligomeric and the polymer coating at least a portion of the surface of the particle cores with the polymer and forming the polymer-coated particles having a solid outer polymeric shell;
substituting the non-aqueous solvent with water to form an aqueous mixture containing the polymer-coated particles; and
forming an aqueous chemical mechanical polishing formulation with an aqueous dispersion of the polymer-coated particles without drying the polymer-coated particles.
7. The method of claim 6 including the additional step of separating the polymer-coated particles as a supernatant.
8. The method of claim 6 wherein the aqueous substituting step relies upon azeotropic distilling to remove the non-aqueous solvent from the polymer-coated particles.
9. The method of claim 6 including the additional step of attaching functional groups to the polymer-coated particles.
10. The method of claim 6 wherein the dispersing particles in the non-aqueous solvent occurs without an earlier solvent transfer from an aqueous solution.