Author: claimsbot

1. A method of making a baked product having first and second outer regions opposing each other and an intermediate region interposed therebetween in a baking mold including a first exterior element having at least one first baking cavity, a second exterior element having at least one second baking cavity, and an intermediate supportive element disposed between the first and second exterior elements and having at least one operational channel so that said at least one first baking cavity, said at least one second baking cavity, and said at least one operational channel define at least one baking chamber, said method comprising:
at least partially filling said at least one baking chamber with dough, so as to form said first and second outer regions and said intermediate region respectively within each of said at least one first baking cavity, said at least, one second baking cavity and said at least one operational channel;
exposing said at least one baking chamber of the baking mold to an outside heat causing initial baking and solidifying of said dough within said at least one baking chamber;
directly exposing said oppositely disposed first and second outer regions of the product to said outside heat;
supporting said product by said at least one operational channel of said intermediate supportive element; and
continuing baking said product so as to form a crust on said first and second oppositely disposed outer regions.
2. The method according to claim 1, wherein, in said exposing said at least one baking chamber of the baking mold to an outside heat, formation of the crust being prevented or minimized in said first and second oppositely disposed outer regions of the baked product.
3. The method according to claim 2, wherein:
said at least one first baking cavity and said at least one second baking cavity each comprises a mesh screen; and
said crust formation is manipulated by slowly raising the temperature within the at least one baking chamber so as to minimize the temperature variation between inside and outside of the dough and to allow developed gases to escape from the at least one baking chamber through said mesh screen.
4. The method according to claim 1, further comprising detecting a condition of said dough within said at least one baking chamber prior to said directly exposing.
5. The method according to claim 4, further comprising ejecting a finally baked product from said at least one operational channel of said intermediate supportive element.
6. A method according to claim 5, wherein:
said intermediate supportive element is defined by at least a pair of supportive members movably connected to each other;
each of said at least the pair of supportive members is formed with at least one recess; and
corresponding recesses of the at least the pair of supportive members are configured to define, in combination, said at least one respective operational channel, such that in said ejecting the finally baked product, supportive members are separated, facilitating ejection of said finally baked product from said intermediate supportive element.
7. The method according to claim 6, wherein each of said at least the pair of supportive members is formed with recesses forming, in combination, corresponding operational channels.
8. The method according to claim 1, wherein, in said at least partially filling said at least one baking chamber with dough, said first outer region of the baked product is formed within said at least one first baking cavity of the first exterior element and said second outer region is formed within said at least one second baking cavity of the second exterior element, and said intermediate region is formed within said at least one operational channel of said intermediate supportive element.
9. The method according to claim 6, wherein, in said detecting the condition of said dough, upon detecting of a predetermined degree of viscosity of the dough within said at least one baking chamber, a signal is generated causing separation of said supportive members.
10. The method according to claim 7, wherein said first exterior element is provided with a plurality of the first baking cavities and said second exterior element is formed with a plurality of the second baking cavities, so that a plurality of continuous baking chambers is formed within said baking mold.
11. A method of producing a baked product, comprising:
removably interconnecting a first structural portion, a second structure portion and an intermediate structural portion such that said intermediate structural portion is interposed between said first and second structural portions, said intermediate structural portion defining an operational channel extending between a first channel opening and a second channel opening of said intermediate structural portion, said first structural portion including a first hollow region having a first opening and said second structural portion including a second hollow region having a second opening, said removably interconnecting including bringing said first opening and said second opening of said first and second structural portions each into communication with a corresponding one of said first channel opening and said second channel opening such that said operational channel provides communication between said first hollow region of said first structural portion and said second hollow region of said second structural portion, said first hollow region, said second hollow region and said operational channel thereby collectively defining at least a portion of a baking chamber;
at least partially filling said baking chamber with a desired amount of a dough;
baking the dough in said baking chamber by exposing said baking chamber to heat;
creating a separation between said first opening and said second opening of said first and second structural portions and the corresponding one of each of said first and second channel openings when the dough is formed into a partially baked product which is sufficiently solidified to be supportable by the operational channel of the intermediate structure to thereby directly expose to baking conditions extended portions of the partially baked product which protrude from said first and second channel openings and which were formed within said first and second hollow regions during said partially baking; and
continuing said baking to transform the partially baked product into the baked product having a crust on said extended portions thereof.
12. A method of preparing a baked product, comprising:
filling a desired amount of a dough in a baking chamber;
baking the dough in said baking chamber by exposure thereof to heat to initially form a partially baked product which is sufficiently firm so as to be self-supportive;
directly exposing extended portions of the partially baked product located outward of a central portion of the partially baked product to the ambient heat; and
continuing the baking to transform the partially baked product into the baked product having a crust formed on the extended portions.
13. A method of preparing a baked product, comprising:
providing a baking chamber comprised of first and second outer chamber portions and an intermediate chamber portion interposed between said first and second outer chamber portions;
at least partially filling said baking chamber with a desired amount of a dough;
baking the dough in said baking chamber by exposure thereof to heat to form a partially baked product including an intermediate product portion being disposed within said intermediate chamber portion and extended product portions which protrude from said intermediate chamber portion into said first and second outer chamber portions;
exposing said extended product portions of the partially baked product directly to said heat by moving said first and second outer chamber portions away from said intermediate chamber portion when the partially baked product is sufficiently solidified to be self-supportable in the intermediate chamber portion; and
continuing baking the partially baked product to form the baked product which includes a crust formed on each of said extended portions thereof.
14. The method according to claim 13, wherein said at least partially filling includes injecting the dough through an opening in structure defining the baking chamber.
15. The method according to claim 13, further comprising detecting a critical point in the baking for proceeding with said directly exposing, said detecting including measuring a viscosity of the partially baked product.
16. The method according to claim 13, wherein said baking the dough in said baking chamber with the extended product portions which protrude from said intermediate chamber into said first and second outer chamber portions includes inhibiting crust formation before said directly exposing is carried out.
17. The method according to claim 13, wherein at least a portion of structure defining the baking chamber is in a form of a mesh.
18. The method according to claim 13, wherein moving said first and second outer chamber portions away from said intermediate chamber portion is initiated in response to detecting that the partially baked product is sufficiently solidified to be self-supportable by the intermediate chamber portion.
19. The method according to claim 16, wherein said inhibiting includes defining said first and second outer chamber portions by a structure having a low specific heat which surrounds the partially baked product on all sides.
20. The method according to claim 16, wherein said inhibiting includes slowly raising a temperature of from a time when the dough in said baking chamber is exposed thereto, so as to minimize a difference in temperature between an inside and an outside of the dough.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A shell structure with a ring clip, the shell structure having a retainer hole bored therethrough, characterized by:
the ring clip comprising a head portion and an adjoining neck portion, the head portion being exposed outside the shell structure, the neck portion extending through the retainer hole of the shell structure and hooking a inner wall of the shell structure, the neck portion having a cone shape with a slope, and the ring clip capable of rotating freely relative to the shell body.
2. The shell structure with a ring clip of claim 1, wherein the head portion has a through slot for insertion by an external string.
3. The shell structure with a ring clip of claim 1, wherein the head portion includes a skirt formed at the bottom thereof, the diameter of the skirt larger than the diameter of the retainer hole for preventing the head portion from falling into the shell body.
4. The shell structure with a ring clip of claim 1, wherein the neck portion further comprises a retainer portion extending from the bottom of the neck portion.
5. The shell structure with a ring clip of claim 1, wherein the neck portion is gradually shrunk from the interior of the shell body to the exterior of the shell body.
6. The shell structure with a ring clip of claim 1, wherein the neck portion is gradually shrunk from the exterior of the shell body to the interior of the shell body.
7. The shell structure with a ring clip of claim 1, wherein the height of the neck portion is longer than the depth of the retainer hole of the shell body for enabling the neck portion to move slightly back and forth relative to the shell body.

1. A method comprising:
at a data storage device including a resistive random access memory (ReRAM), performing:
storing data in the ReRAM by performing a first number of write operations to a storage region of the ReRAM, the storage region tracked by a counter; and
incrementing a value of the counter a second number of times responsive to storing the data in the storage region, wherein the second number is different than the first number.
2. The method of claim 1, further comprising determining a ratio between a number of cells programmed by a first write operation of the write operations and a normalization parameter.
3. The method of claim 2, wherein the normalization parameter corresponds to a data block size of the ReRAM.
4. The method of claim 3, wherein the first write operation writes first data to the ReRAM, the first data having the data block size.
5. The method of claim 4, wherein the write operations further include a second write operation, and wherein the second write operation writes second data to the ReRAM, the second data having a data size that is less than the data block size.
6. The method of claim 5, wherein the second data corresponds to a modification of one or more values of the first data.
7. The method of claim 2, further comprising, for each of the write operations, determining a number such that a probability of the number being equal to one corresponds to the ratio.
8. The method of claim 7, wherein the value of the counter is incremented by a controller of the data storage device each time the number is equal to one.
9. The method of claim 1, wherein the value of the counter approximates a number of programerase (PE) cycles associated with the storage region of the ReRAM.
10. The method of claim 1, wherein a storage capacity of the storage region is less than a total storage capacity of the ReRAM, and wherein the second number is less than the first number.
11. The method of claim 1, wherein the ReRAM has a three-dimensional (3D) memory configuration.
12. A data storage device comprising:
a resistive random access memory (ReRAM);
a counter configured to track a storage region of the ReRAM; and
a controller, wherein the controller is coupled to the ReRAM, wherein the controller is configured to perform a first number of write operations to the storage region and to increment a value of the counter a second number of times responsive to storing data in the storage region, wherein the second number is less than the first number.
13. The data storage device of claim 12, wherein the controller is further configured to determine a ratio between a number of cells programmed by a first write operation of the write operations and a normalization parameter.
14. The data storage device of claim 13, wherein the value of the counter statistically indicates a number of programerase (PE) cycles associated with the storage region.
15. The data storage device of claim 13, wherein the normalization parameter corresponds to a data block size of the ReRAM.
16. The data storage device of claim 15, wherein the first write operation writes first data to the ReRAM, and wherein the first data has the data block size.
17. The data storage device of claim 16, wherein the write operations further include a second write operation, and wherein the second write operation writes second data to the ReRAM, the second data having a data size that is less than the data block size.
18. The data storage device of claim 13, wherein the controller includes a pseudo-random number generator (PRNG) that is configured to generate a number, for each of the write operations, such that a probability of the number being equal to one corresponds to the ratio.
19. The data storage device of claim 12, wherein the controller further includes multiple counters corresponding to multiple storage regions of the ReRAM.
20. The data storage device of claim 12, further comprising a memory die, wherein the memory die includes the ReRAM, and wherein the controller includes the counter.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A polypeptide comprising an amino acid sequence of SEQ ID NO: 6, wherein the polypeptide retains a net negative charge of 1-8.
2. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 2-8.
3. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 3-8.
4. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 4-8.
5. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 5-8.
6. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 6-8.
7. The polypeptide of claim 1 wherein the polypeptide retains a net negative charge of 7-8.
8. The polypeptide of claim 1 wherein amino acid residue sixteen is serine.
9. The polypeptide of claim 1 wherein amino acid residue twenty-one is norleucine.
10. The polypeptide of claim 1 which comprises the amino acid sequence of SEQ ID NO: 1.
11. A composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier.
12. The polypeptide of claim 1 consisting of the sequence of SEQ ID NO: 1.
13. The polypeptide of claim 1 wherein the polypeptide is fused to a membrane-penetrating peptide.
14. The polypeptide of claim 13 wherein the membrane-penetrating peptide is selected from the group consisting of: VP-22 (SEQ ID NO: 3), (SEQ ID NO: 4), and (SEQ ID NO: 5).
15. A method of activating a CFTR protein comprising:
administering an effective amount of a polypeptide to a cell comprising a CFTR protein which forms a cAMP-regulated chloride channel, said polypeptide comprising the sequence of SEQ ID NO: 6, whereby the CFTR protein is activated.
16. The method of claim 15 wherein the polypeptide comprises the sequence of SEQ ID NO: 1.
17. The method of claim 15 wherein the effective amount of the polypeptide increases open probability of the channel formed by the CFTR by at least 25%.
18. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 50%.
19. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 75%.
20. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 100%.
21. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 125%.
22. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 150%.
23. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 175%.
24. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 200%.
25. The method of claim 15 wherein open probability of the channel formed by the CFTR increases by at least 300%.
26. The method of claim 15 wherein said polypeptide is administered to achieve a concentration of 0.5 to 14 M.
27. The method of claim 15 wherein said polypeptide is administered to achieve a concentration of 4-6 M.
28. The method of claim 15 wherein the CFTR protein is a mutant which reaches the cell’s plasma membrane but fails to undergo full activation in the absence of said polypeptide.
29. The method of claim 28 wherein the mutant CFTR protein is selected from the group consisting of 816CT, 741TG, 471delAGG, 363CT, 102TA, 94GT, 33GA 132CG, P5L, S10R, S13F, 1851GT, 1854AT, 18613CG, W19C, G27E, R31C, R31L, 232del18, S42F, D44G, A46D, 279AG, I50T, S50P, S50Y, 2963insT, 2961GT, 2961GC, 2962TC, 2969AT, 29612TC, 29728insA, 2973CA, 2973CT, 2972AG, 29710TG, 29712insA, E56K, W57G, W57R, D58N, D58G, E60K, E60L, N66S, P67L, K68E, K68N, A72T, A72D, R74W, R74Q, R75L, W79R, G85E, G85V, F87L, L88S, Y89C, L90S, G91R, 4051GA, 4053AC, 4054AG, 40610CG, 4066TC, 4063TC, 4062AG, 4062AC, 4061GC, 4061GA, 4061GT, E92K, A96E, Q98R, P99L, I105N, S108F, Y109N, Y109C, D110H, D110Y, D110E, P111A, P111L, delta E115, E116Q, E116K, R117C, R117H, R117P, R117L, A120T, I125T, G126D, L137R, L137H, L138ins, H139R, P140S, P140L, A141D, H146R, I148T, I148N, G149R, M152V, M152R, 591del18, A155P, S158R, Y161N, Y161D, Y161S, K162E, 621GA, 6211GT, 621TC, 6212TG, 6213AG, 6222AC, 6221GA, L165S, K166Q, R170C, R170G, R170H, I175V, I177T, G178R, Q179K, N186K, N187K, D192N, delta D192, D192G, E193K, 7111GT, 7113AC, 7113AG, 7113AT, 7115GA, 71134AG, 7121GT, G194V, A198P, H199Y, H199Q, V201M, P205S, L206W, L206F, A209S, E217G, Q220R, C225R, L227R, V232D, Q237E, G239R, G241R, M243L, M244K, R248T, 8751GC, 8751GA, 87614del12, 87610del8, 8763CT, R258G, V920L, M265R, E278del, N287Y, 994del9, 10023TG, E292K, R297W, R297Q, A299T, Y301C, S307N, A309D, A309G, delta F311, F311L, G314R, G314V, G314E, F316L, V317A, L320V, L320F, V322A, L327R, R334W, R334L, R334Q, I336K, T338I, E474K, L346P, R347C, R347H, R347P, R347L, M348K, A349V, R352W, R352Q, Q353H, Q359KT360K, Q359R, W361R(TC), W361R(TA), S364P, L365P, 1243ins6, 12481GA, 124929delAT, 124927delTA, 12495AG, L375F, E379X, L383S, T360R, V392A, V392G, M394R, A399V, E403D, 1341GA, 1341GA, 13411GA, 134118AC, 134211TTTG, 13422AC, 13421GC, E407V, N418S, G424S, D443Y, I444S, Q452P, delta L453, A455E, V456F, G458V, 15246insC, 15251GA, S466L, G480S, G480C, G480D, H484Y, H484R, S485C, C491R, S492F, Q493R, P499A, T501A, I502T, E504Q, I506L, delta I507, I506S, I506T, delta F508, F508S, D513G, Y517C, V520F, V520I, 1706del16, 1706del17, E527Q, E527G, 17161GA, E528D, 17162TC, 17178GA, 17173TG, 17172AG, 17171GA, 17179TA, D529H, A534E, I539T, G544S, G544V, S549R(AC), S549N, S549I, S549R(TG), G550R, G551S, G551D, Q552K, R553G, R553Q, R555G, I556V, L558S, A559T, A559E, R560K, R560T, 18111GC, 18111.6kbAG, 181118GA, 18121GA, R560S, A561E, V562L, V562I, Y563D, Y563N, Y563C, L568F, Y569D, Y569H, Y569C, L571S, D572N, P574H, G576A, Y577F, D579Y, D579G, D579A, T582I, T582R, S589N, S589I, 18981GT, 18981GC, 18981GA, 18983AC, 18983AG, 18985GT, 18985GA, 189873TG, R600G, I601F, V603F, T604I, 1949del84, H609R, L610S, A613T, D614Y, D614G, I618T, L619S, H620P, H620Q, G622D, G628R(GA), G628R(GC), L633P, L636P, D648V, D651N, T665S, E672del, K683R, F693L(CTT), F693L(TTG), K698R, E725K, P750L, V754M, T760M, R766M, N782K, R792G, A800G, E822K, E826K, 26221GT, 26221GA, 26222del6, D836Y, R851L, C866Y, L867X, 2751GA, 27512TA, 27513AG, 275226AG, 27521GT, 27521GC, T908N, 27892insA, 27893delG, 27895GA, 27902AG, 27901GC, 27901GT, Q890R, D891G, S895T, T896I, N900T, 2851AG, S912L, Y913C, Y917D, Y917C, I918M, Y919C, V920M, D924N, L927P, F932S, R933S, V938G, H939D, H939R, S945L, S945L, K946X, H949Y, H949R, M952T, M952I, M961I, L967S, G970R, 30402TC, 30411GA, G970D, L973F, L973P, S977P, S977F, D979V, D979A, I980K, D985H, D985Y, I991V, D993Y, F994C, 3120GA, 31201GA, 31212AT, 31212AG, 31211GA, L997F, 3131del15, I1005R, A1006E, V1008D, A1009T, P1013L, Y1014C, P1021S, 3195del6, 3196del54, 3199del6, I1027T, M1028R, M1028I, Y1032C, I1366T, 3271delGG, 32711GA, 32711delGG, 327226AG, 32729AT, 32724AG, 32721GA, G1047D, F1052V, T1053I, T1053I, H1054D, T1057A, K1060T, G1061R, L1065F, L1065R, L1065P, R1066S, R1066C, R1066H, R1066L, A1067T, A1067D, G1069R, R1070W, R1070Q, R1070P, Q1071P, Q1071H, P1072L, F1074L, L1077P, H1085R, T1086I, N1088D, Y1082H, L1093P, L1096R, W1098R, Q1100P, M1101R, M1101K, S1118F, S1118C, G1123R, 34992TC, 34993AG, 34996AG, 35002AG, E1123del, G1127E, 3523AG, A1136T, M1137V, M1137R, I1139V, delta M1140, M1140K, T1142I, V1147I, N1148K, D1152H, V1153E, D1154G, 3600GA, 36002insT, 36005GA, 360120TC, 360117TC, 36012AG, S1159P, S1159F, D1168G, K1177R, 3696GA, V1190P, 3750delAG, 3755delG, M1210I, V1212I, L1227S, E1228G, I1230T, I1234V, S1235R, G1237S, Q1238R, 3849GA, 38491GA, 38494AG, 384910kbCT, 38495GA, 38503TG, 38501GA, V1240G, G1244V, G1244E, T1246I, G1247R, G1249R, G1249E, S1251N, T1252P, S1255P, S1255L, F1257L, delta L1260, 3922del10C, I1269N, D1270N, W1282G, W1282R, W1282C, R1283M, R1283K, F1286S, Q1291R, Q1291H, 40051GA, 40052TC, 400661del14, 400619del3, 400614CG, 40068TA, 40064AG, V1293I, T12991, F1300L, N1303H, N1303I, N1303K, D1305E, Q1313K, V1318A, E1321Q, 409628GA, 40963CG, L1335P, F1337V, L1339F, G1349S, G1349D, K1351E, Q1352H*, R1358S, A1364V, D1377H, L1388Q, V1397E, E1409V, Q1412X, 437410TC, 43741GA, 43741GT, 43751GC, R1422W, S1426P, D1445N, R1453W, CFTRdele14a, CFTRdele19, 2104insA21092118del10, and CF25kbdel as listed in Table 1.
30. The method of claim 15 wherein the polypeptide is administered in an aerosol to a patient with a mutant CFTR protein.
31. The method of claim 15 wherein the polypeptide is administered in an aerosol to a patient with insufficient amounts of wild-type CFTR to maintain chloride transport.
32. The method of claim 30 wherein the aerosolized polypeptide is co-administered with an expression vector wherein said expression vector encodes wild-type CFTR protein.
33. The method of claim 31 wherein the aerosolized polypeptide is co-administered with an expression vector wherein said expression vector encodes wild-type CFTR protein.
34. A method of activating a CFTR protein comprising:
applying an effective amount of a polypeptide to a CFTR protein in a lipid bilayer wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 6, whereby the CFTR protein is activated.
35. The method of claim 34 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 1.
36. The method of claim 34 further comprising measuring a change in conductance upon applying the polypeptide.
37. A method of synthesizing a CFTR activating polypeptide comprising:
sequentially linking units of one or more amino acid residues to form a polypeptide comprising the amino acid sequence of SEQ ID NO: 6.
38. The method of claim 37 wherein F-moc synthesis is used.
39. The method of claim 37 wherein the polypeptide has the sequence of SEQ ID NO: 1.
40. A polypeptide comprising the amino acid sequence as shown in SEQ ID NO: 2.
41. The polypeptide of claim 40 wherein the polypeptide is fused to a membrane-penetrating peptide.
42. The polypeptide of claim 41 wherein the membrane-penetrating peptide is selected from the group consisting of: VP-22 (SEQ ID NO: 3), (SEQ ID NO: 4) and (SEQ ID NO: 5).
43. A nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide according to SEQ ID NO: 2.
44. A method of activating a CFTR protein, comprising:
administering a nucleic acid comprising a sequence encoding a polypeptide according to SEQ ID NO: 2 to a cell comprising the CFTR protein, whereby the polypeptide is expressed and the CFTR protein is activated.
45. The method of claim 44 wherein the cell is in a patient and the nucleic acid is administered as an aerosol to the patient’s airways.
46. The method of claim 45 wherein the nucleic acid molecule is co-administered with an expression vector encoding a wild-type CFTR protein.