Author: claimsbot

What we claim:

1. In a cell culture medium comprising an aqueous solution of amino acids and a carbohydrate energy source for cells cultured in said medium,
the improvement wherein said aqueous solution
(i) is substantially saturated with said amino acids at a temperature of 30 to 50 C.;
(ii) has an osmolarity of 320 to 450 mOsm;
(iii) contains 5.50 to 20 grams per liter of amino acids and 5.50 to 20 grams per liter of said carbohydrate energy source
and wherein the dry weight of the amino acids in solution in said medium comprises at least 20% of the total dry weight of all solid components present in said medium.
2. The claim 1 cell culture medium in which said carbohydrate energy source is glucose.
3. The claim 1 cell culture medium in which said osmolarity of from 320 to 450 mOsm is provided at least in part by sodium salt.
4. The claim 1 or claim 2 cell culture medium in which said osmolarity of from 320 to 450 mOsm is provided at least in part by a sodium chloride osmolyte.
5. The claim 1 cell culture medium further comprising a suspension of undissolved amino acids in said aqueous solution substantially saturated with amino acids
wherein said substantial saturation is maintained as cell growth consumes amino acids in solution in said aqueous medium.
6. In an aqueous basal cell culture medium, the improvement which comprises providing in said basal medium
(i) a concentration of one or more amino acids reactive with cell transport system A to provide a total amino acid concentration of 5.50 to 20 grams per liter;
(ii) an osmolarity of from 320 to 420 mOsm
said osmolarity being provided by a sodium salt osmolyte.
7. The claim 6 cell culture medium in which the osmolyte (ii) is sodium chloride.
8. The claim 6 cell culture medium in which said one or more amino acids reactive with cell transport system A are zwitterionic amino acids.
9. The claim 6 cell culture medium in which said one or more amino acids reactive with cell transport system A are selected from the group consisting of alanine, glycine, histidine, methionine, proline and serine.
10. The claim 6 or claim 7 or claim 8 cell culture medium in which said basal cell culture medium is RPMI, DMEM, Ham’s F12, RDF or eRDF.
11. The cell culture medium 28101, 28102 or 28103.
12. The method which comprises culturing a cell in the culture medium of claim 1, claim 2, or claim 3, or claim 11.
13. A method which comprises:
(i) providing the cell culture medium of claim 1 or claim 2 or claim 3 or claim 11;
(ii) culturing a CHO cell containing a recombinant expression vector in the medium provided in step (i); and
(iii) recovering the recombinant protein expressed by said CHO cell.
14. The claim 13 method in which the CHO cell containing a recombinant expression vector is a dicistronic DHFR intron expression vector.
15. A method which comprises culturing a CHO cell in culture medium 28103.
16. The method of claim 13 wherein said cell cultured in said culture medium is a hybridoma.
17. The method which comprises culturing a mammalian cell in the culture medium of claim 1, claim 2 or claim 3 or claim 11.
18. The method which comprises culturing a mammalian cell having an expression vector for a recombinant protein in a culture medium of claim 1, claim 2 or claim 3 or claim 11.
19. The method which comprises culturing a CHO cell or a BHK cell or a CO5 cell or a Namaliva cell having expression vector for a recombinant protein in a culture medium of claim 1, claim 2, claim 3 or claim 11.
20. The method which comprises culturing a cell in the culture medium of claim 6 or claim 7 or claim 8 or claim 9.
21. The method which comprises culturing a mammalian cell in the culture medium of claim 7 or claim 8 or claim 9.
22. The method which comprises culturing a mammalian cell having an expression vector for a recombinant protein in a culture medium of claim 7 or claim 8 or claim 9.
23. The method which comprises culturing a CHO cell or a BHK cell or a CO5 cell or a Namaliva cell having expression vector for a recombinant protein in a culture medium of claim 7 or claim 8 or claim 9.
24. A dry mixture of cell culture medium components comprising amino acids, carbohydrates and vitamins,
wherein said amino acids component comprise at least 20% of the dry weight of said mixture.
25. The claim 24 mixture wherein said amino acids component comprises from 30% to 50% of the dry weight of said mixture.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An deployable template structure comprising:
a template including at least one of the following:
a virtual machine (VM) section to describe possible virtual machines deployable on a system;
a filter section to describe one or more filters applicable to components of the system; and
a component configuration settings section to describe properties of deployable engine components.
2. The deployable template structure of claim 1, wherein the virtual machine section of the template includes levels for at least one of the following:
a VM vendor level to describe at least one possible deployable VM vendors;
a platform level to describe at least one possible platform of a specific possible deployable VM vendor; and
a global parameter level to describe parameters applicable to any possible deployable VM vendor and platform.
3. The deployable template structure of claim 1, wherein a filter of the filter section includes:
an action to be performed on a component;
a component type;
a vendor name; and
a component name.
4. The method of claim 3, wherein the action to be performed is selected from the group consisting of:
start; and
stop.
5. The method of claim 4, wherein the component type is selected from the group consisting of:
service;
library;
interface; and
application.
6. The deployable template structure of claim 1, wherein the component configuration settings section includes properties for each type of deployable engine component.
7. The deployable template structure of claim 1, wherein at least one of the sections of the template are XML documents.
8. A method comprising:
acquiring properties of a first configuration profile, the first configuration profile defining a structure of an instance’s configuration values;
acquiring values of a second configuration profile, the second configuration profile further defining values for the structure of the instance’s configuration;
inheriting the properties of the first configuration profile, wherein the second configuration profile inherits the properties of the first configuration profile; and
applying the values of the second configuration profile to the first configuration profile;
acquiring the properties of a third configuration profile, the third configuration profile defining a default template applicable to multiple instances on a single system, wherein the default template includes:
a virtual machine (VM) section to describe possible virtual machines deployable on the system,
a filter section to describe filters applicable to components of the system, and
a component configuration settings section to describe properties of deployable engine components;

the third configuration profile inheriting the properties of the first and second configuration profiles; and
applying the sections of the third configuration profile to the inherited profiles.
9. The method of claim 8, further comprising:
acquiring the properties of a fourth configuration profile, the fourth configuration profile further defining the use case scenario;
inheriting the properties of the first, second, and third configuration profiles, wherein the fourth configuration profile inherits the properties of the first, second, and third configuration profiles; and
applying the values of the fourth configuration profile to the inherited profiles.
10. The method of claim 9, further comprising:
acquiring the properties of a fifth configuration profile, the fifth configuration profile further defining a specific instance;
inheriting the properties of the first, second, third, and fourth configuration profiles, wherein the fifth configuration profile inherits the properties of the first, second, third, and fourth configuration profiles; and
applying the values of the fifth configuration profile to the inherited profiles.
11. The method of claim 8, wherein the configuration profiles are written in XML.
12. An article of manufacture including program code which, when executed by a machine, causes the machine to perform a method, the method comprising:
acquiring properties of a first configuration profile, the first configuration profile defining a structure of an instance’s configuration values;
acquiring values of a second configuration profile, the second configuration profile further defining values for the structure of the instance’s configuration;
inheriting the properties of the first configuration profile, wherein the second configuration profile inherits the properties of the first configuration profile; and
applying the values of the second configuration profile to the first configuration profile;
acquiring the properties of a third configuration profile, the third configuration profile defining a default template applicable to multiple instances on a single system, wherein the default template includes:
a virtual machine (VM) section to describe possible virtual machines deployable on the system,
a filter section to describe filters applicable to components of the system, and
a component configuration settings section to describe properties of deployable engine components;

the third configuration profile inheriting the properties of the first and second configuration profiles; and
applying the sections of the third configuration profile to the inherited profiles.
13. The method of claim 12, further comprising:
acquiring the properties of a fourth configuration profile, the fourth configuration profile further defining the use case scenario;
inheriting the properties of the first, second, and third configuration profiles, wherein the fourth configuration profile inherits the properties of the first, second, and third configuration profiles; and
applying the values of the fourth configuration profile to the inherited profiles.
14. The method of claim 13, further comprising:
acquiring the properties of a fifth configuration profile, the fifth configuration profile further defining a specific instance;
inheriting the properties of the first, second, third, and fourth configuration profiles, wherein the fifth configuration profile inherits the properties of the first, second, third, and fourth configuration profiles; and
applying the values of the fifth configuration profile to the inherited profiles.
15. The method of claim 12, wherein the configuration profiles are written in XML.

1. A method of implanting an annuloplasty ring in a mitral valve annulus having an anterior aspect and a posterior aspect, the annulus having a dilated pathology symptomized by lengthening of the anterior-posterior dimension and a subsequent loss of coaptation between the posterior and anterior leaflets thus causing mitral regurgitation, the annulus further exhibiting a drop of the posterior aspect and consequent relaxation of the posterior muscle wall of the left ventricle, the method comprising:
providing a generally oval-shaped annuloplasty ring having an anterior portion adapted to be implanted on the anterior aspect of the mitral valve annulus, a posterior portion opposite the anterior portion adapted to be implanted on the posterior aspect of the mitral valve annulus, right and left sides between the anterior and posterior portions, and transition segments between the sides and the posterior portion;
wherein the annuloplasty ring is oriented about a central axis having an upward direction and a downward direction, the downward direction corresponding to the direction of blood flow through the mitral valve annulus, the ring having in plan view perpendicular to the central axis a longer dimension along a major axis than a shorter dimension along a minor axis, the ring posterior portion having an upward bow that rises upward to an axial position higher than the remainder of the ring; and,
securing the annuloplasty ring with the anterior portion against the annulus anterior aspect and the posterior portion against the annulus posterior aspect so that the ring posterior portion elevates the annulus posterior aspect and re-shapes the geometry of the left ventricle.
2. The method of claim 1, wherein the posterior portion extends radially inward from the adjacent transition segments to a radial position along the minor axis that is closer to the central axis than an imaginary posterior projection in plan view of the sides toward each other, the method including reconfiguring the annulus accordingly.
3. The method of claim 1, wherein the ring is substantially saddle-shaped with the sides curving upward between the anterior portion and adjacent transition segments, the method including reconfiguring the annulus accordingly.
4. The method of claim 1, wherein the ring is generally planar except for the posterior portion, the method including reconfiguring the annulus accordingly.
5. The method of claim 1 wherein the sides and transition segments are generally curvilinear and the junctures between adjacent sides and transition segments are generally rounded, the method including reconfiguring the annulus accordingly.
6. The method of claim 1, wherein the posterior portion extends radially inward from the adjacent sides to a radial position along the minor axis that is closer to the central axis than an imaginary posterior projection in plan view of the sides toward each other, the method including reconfiguring the annulus accordingly.
7. The method of claim 1, wherein the ring body is comprised of a material having a high modulus of elasticity that will substantially resist distortion when subjected to the stress imparted thereon when the ring is implanted.
8. A method of correcting a mitral valve annulus having a dilated pathology symptomized by lengthening of dimension between an anterior aspect and a posterior aspect thereof, and a subsequent loss of coaptation between posterior and anterior leaflets thus causing mitral regurgitation, the annulus further exhibiting a drop of the posterior aspect and consequent relaxation of a posterior muscle wall of the left ventricle, the method comprising:
providing an annuloplasty ring made of a material having a high modulus of elasticity that will substantially resist distortion when subjected to the stress imparted thereon when the ring is implanted in the mitral valve annulus of an operating human heart, the annuloplasty ring having an anterior portion and a posterior portion with an upward bow; and,
securing the annuloplasty ring with the anterior portion against the annulus anterior aspect and the posterior portion against the annulus posterior aspect so that the ring posterior portion elevates the annulus posterior aspect above its normal elevation and consequently re-shapes the geometry of the left ventricle.
9. The method of claim 8, wherein the annuloplasty ring is comprised of a ceramic material.
10. The method of claim 9, wherein the annuloplasty ring is comprised of Stellite.
11. A method of implanting an annuloplasty ring in a mitral valve annulus having an anterior aspect and a posterior aspect, the annulus having a dilated pathology symptomized by lengthening of the anterior-posterior dimension and a subsequent loss of coaptation between the posterior and anterior leaflets thus causing mitral regurgitation, the method comprising:
providing an annuloplasty ring made of a material having a high modulus of elasticity that will substantially resist distortion when subjected to the stress imparted thereon when the ring is implanted in the mitral valve annulus of an operating human heart, wherein the ring is oriented about a central axis having an upward direction and a downward direction, the downward direction corresponding to the direction of blood flow through the mitral valve annulus, the ring having a posterior portion that extends both radially inward and axially upward from the remainder of the ring; and
securing the annuloplasty ring with the anterior portion against the annulus anterior aspect and the posterior portion against the annulus posterior aspect so that the ring posterior portion elevates and pulls radially inward the annulus posterior aspect.
12. The method of claim 11, wherein the ring body has an anterior portion, a posterior portion opposite the anterior portion, right and left sides between the anterior and posterior portions, and transition segments between the sides and the posterior portion, and wherein the ring body is substantially saddle-shaped with the sides curving upward between the anterior portion and adjacent transition segments, the method including reconfiguring the annulus accordingly.
13. The method of claim 12, wherein a mid-section of the posterior portion bows upward from the adjacent transition segments to an axial position higher than the highest axial position of either of the right or left sides, the method including reconfiguring the annulus accordingly.
14. The method of claim 12, wherein the right and left sides each rise upward from the adjacent transition segments to an axial position above the highest axial position of the posterior portion, the method including reconfiguring the annulus accordingly.
15. The method of claim 11, wherein the ring body is generally planar except for the upwardly bowed posterior portion, the method including reconfiguring the annulus accordingly.
16. The method of claim 11, wherein the ring body has an anterior portion, a posterior portion opposite the anterior portion, right and left sides between the anterior and posterior portions, and transition segments between the sides and the posterior portion, and wherein the posterior portion extends radially inward from the adjacent transition segments to a radial position along the minor axis that is closer to the central axis than an imaginary posterior projection in plan view of the sides toward each other, the method including reconfiguring the annulus accordingly.
17. The method of claim 11, wherein the ring body has an anterior portion, a posterior portion opposite the anterior portion, right and left sides between the anterior and posterior portions, and transition segments between the sides and the posterior portion, and wherein the posterior portion extends radially inward from the adjacent sides to a radial position along the minor axis that is closer to the central axis than an imaginary posterior projection in plan view of the sides toward each other, the method including reconfiguring the annulus accordingly.
18. A method of implanting an annuloplasty ring in a mitral valve annulus having an anterior aspect and a posterior aspect, the annulus having a dilated pathology symptomized by lengthening of the anterior-posterior dimension and a subsequent loss of coaptation between the posterior and anterior leaflets thus causing mitral regurgitation, the method comprising:
providing an annuloplasty ring made of a material having a high modulus of elasticity that will substantially resist distortion when subjected to the stress imparted thereon when the ring is implanted in the mitral valve annulus of an operating human heart, the annuloplasty ring having an anterior portion and a posterior portion that extends both radially inward and axially upward from the remainder of the ring; and,
securing the annuloplasty ring with the anterior portion against the annulus anterior aspect and the posterior portion against the annulus posterior aspect so that the ring posterior portion elevates and pulls radially inward the annulus posterior aspect and corrects the mitral regurgitation.
19. The method of claim 18, wherein the annuloplasty ring is comprised of a ceramic material.
20. The method of claim 19, wherein the annuloplasty ring is comprised of Stellite.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An ink jet recording head comprising:
a recording element substrate provided with an energy generating element for generating energy for ejecting ink from an ink ejection outlet;
an electric wiring member electrically connected with said recording element substrate;
a mounting portion for mounting said recording element substrate; and
an opening provided in said mounting portion,
wherein said electric wiring member penetrates said opening,
a contact surface, projected from said mounting portion in an ink ejection direction, is contactable with a capping member for covering the ink ejection outlet,
said opening is located in an area surrounded by a contact portion of the contact surface contactable with the capping member, and
a spacing between said electric wiring member and said opening is sealed by a sealing agent that is used for sealing an electrical connection portion between said recording element substrate and said electric wiring member.
2. An ink jet recording head comprising:
a recording element substrate provided with an energy generating element for generating energy for ejecting ink from an ink ejection outlet;
an electric wiring member electrically connected with said recording element substrate;
a mounting portion for mounting said recording element substrate;
a contact surface, projected from said mounting portion in an ink ejection direction, contactable with a capping member for covering the ink ejection outlet at a contact portion of said contact surface; and
a guiding portion for guiding said electric wiring member from an area surrounded by said contact portion to outside of the area without intersecting said contact portion, said guiding portion being provided in said mounting portion in the area.
3. A head according to claim 2, wherein said guiding portion comprises a slit-like opening.
4. A head according to claim 2, wherein a spacing between said electric wiring member and said guiding portion is sealed by a sealing agent that is used for sealing an electrical connection portion between said recording element substrate and said electric wiring member.