Author: claimsbot

1. An engine control device that controls a two cycle engine for driving a vehicle by using a microcomputer, comprising:
a reverse switch operated for reversing a rotational direction of said engine;
reverse control means for performing reverse control including a process of excessively advancing an ignition position of said engine for reversing the rotational direction of said engine when said reverse switch is operated; and
a light emitting display device which displays that said reverse control is performed,
wherein said reverse control means comprises light emitting display device blinking means for blinking said light emitting display device when said reverse switch is once operated, and operating error preventing means for starting said reverse control when said reverse switch is again operated within a set time after the one operation of said reverse switch, and stopping said reverse control when said reverse switch is not again operated within said set time after the one operation of said reverse switch.
2. The engine ignition control device according to claim 1, wherein said reverse control means further comprises light emitting display device control means for continuously lighting said light emitting display device when the rotational direction of said engine reversed by said reverse control is a vehicle retracting direction, and extinguishing said light emitting display device when the rotational direction of said engine reversed by said reverse control is a vehicle advancing direction.
3. An engine ignition control device that controls a two cycle engine for driving a vehicle by using a microcomputer, comprising:
a reverse switch operated for reversing a rotational direction of said engine;
reverse control means for performing reverse control for reversing the rotational direction of said engine, comprising speed reduction control means for reducing a rotational speed of said engine when said reverse switch is operated, excessive advance control means for excessively advancing an ignition position of said engine when said rotational speed is reduced to an excessive advance start rotational speed, rotational direction determination means for determining the rotational direction of said engine for confirming whether an excessive advance of said ignition position causes the rotational direction of said engine to be reversed, and reverse time initial ignition control means for igniting said engine in the ignition position at a low speed in a state of the rotational direction being reversed when it is confirmed that said rotational direction is reversed; and
a light emitting display device which displays that said reverse control is performed,
wherein said reverse control means comprises light emitting display device blinking means for blinking said light emitting display device when said reverse switch is once operated, operating error preventing means for starting said reverse control when said reverse switch is again operated within a set time after the one operation of said reverse switch, and stopping said reverse control when said reverse switch is not again operated within said set time after the one operation of said reverse switch, and light emitting display device control means for continuously lighting said light emitting display device when said rotational direction determination means determines that the rotational direction of said engine reversed is a vehicle retracting direction, and extinguishing said light emitting display device when said rotational direction determination means determines that the rotational direction of said engine is a vehicle advancing direction.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1-14. (canceled)
15. A signal processing device comprising:
a variable amplifier configured to output a signal, as an output signal thereof, corresponding to an input signal, an offset and a gain of the variable amplifier being able to be adjusted;
a comparing means configured to compare a level of the output signal of the variable amplifier with a plurality of thresholds;
a detecting means configured to detect either or both of a shift of the offset of the variable amplifier and an excess or deficiency of the gain of the variable amplifier based on the result of the comparison executed in the comparing means; and
an adjusting means configured to adjust either or both of the offset and the gain of the variable amplifier based on the result of the detection executed in the detecting means, wherein
the detecting means is also configured to utilize, as the result of the comparison for each of the plurality of thresholds, a number of times selected from a group consisting of a first number of times that the level of the output signal changes from a level below the threshold to a level over the threshold in a predetermined period, the second number of times that the level of the output signal changes from the level over the threshold to the level below the threshold in the predetermined period, and a third number of times which is a sum of the first number of times and the second number of times, said number of times being similarly selected among the plurality of thresholds.
16. The signal processing device according to claim 15, wherein
the detecting means is also configured to detect either or both of the shift of the offset of the output signal and the excess or deficiency of the gain of the output signal based on a combination of the numbers of times as similarly selected among the plurality of thresholds.
17. The signal processing device according to claim 16, wherein
the detecting means is also configured to detect an estimation of the shift of the offset of the output signal based on the combination of the number of times as similarly selected among the plurality of thresholds, and
the adjusting means is also configured to determine an adjusting amount of the offset based on the estimation of the shift of the offset.
18. The signal processing device according to claim 16, wherein
the detecting means is also configured to detect an estimation of the excess or deficiency of the gain of the output signal based on the combination of the numbers of times as similarly selected among the plurality of thresholds, and
the adjusting means is also configured to determine an adjusting amount of the gain based on the estimation of the excess or deficiency of the gain.
19. A bar-code reader comprising:
a sensor;
the signal processing device according to claim 15; and
a decoder, wherein
the signal processing device is also configured to treat an output signal of the sensor as an input signal supplied to the signal processing device, and
the decoder is configured to treat an output signal of the signal processing device as an input signal supplied to the decoder.
20. A signal processing method comprising:
a step in which, a variable amplifier outputs a signal, as an output signal thereof, corresponding to an input signal, an offset and a gain of the variable amplifier being able to be adjusted;
a step in which, a comparing means compares a level of the output signal of the variable amplifier with a plurality of thresholds;
a step in which, a detecting means detects either or both of a shift of the offset of the variable amplifier and an excess or deficiency of the gain of the variable amplifier based on the result of the comparison executed in the comparing means; and
a step in which, an adjusting means adjusts either or both of the offset and the gain of the variable amplifier based on the result of the detection executed in the detecting means, wherein
the detecting means utilizes, as the result of the comparison for each of the plurality of thresholds, a number of times selected from a group consisting of a first number of times that the level of the output signal changes from a level below the threshold to a level over the threshold in a predetermined period, the second number of times that the level of the output signal changes from the level over the threshold to the level below the threshold in the predetermined period, and a third number of times which is a sum of the first number of times and the second number of times, said number of times being similarly selected among the plurality of thresholds.
21. A signal processing program for causing a computer to function as an output signal’s wave shape adjusting device for adjusting an output signal from a variable amplifier configured to output a signal, as an output signal thereof, corresponding to an input signal, an offset and a gain of the variable amplifier being able to be adjusted, the program causing the computer to function as:
a comparing means configured to compare a level of the output signal of the variable amplifier with a plurality of thresholds;
a detecting means configured to detect either or both of a shift of the offset of the variable amplifier and an excess or deficiency of the gain of the variable amplifier based on the result of the comparison executed in the comparing means; and
an adjusting means configured to adjust either or both of the offset and the gain of the variable amplifier based on the result of the detection executed in the detecting means, wherein
the detecting means is also configured to utilize, as the result of the comparison for each of the plurality of thresholds, a number of times selected from a group consisting of a first number of times that the level of the output signal changes from a level below the threshold to a level over the threshold in a predetermined period, the second number of times that the level of the output signal changes from the level over the threshold to the level below the threshold in the predetermined period, and a third number of times which is a sum of the first number of times and the second number of times, said number of times being similarly selected among the plurality of thresholds.

1-17. (canceled)
18. A method, within an application server, of modifying an intra-transactional cache, comprising:
determining whether a copy of an original first data entity, accessed in the intra-transactional cache by a first client, has been updated;
determining whether the copy is also within an inter-transactional cache;
upon the copy not being within the inter-transactional cache, adding the copy to the inter-transactional cache; and
after the copy has been added to the inter-transactional cache, removing, from the intra-transactional cache, the copy.
19. The method of claim 18, further comprising:
upon a determination that the copy has been updated,
changing a version identifier associated with the original first data entity,
generating a raw data object based upon the updated copy, and
writing the raw data object to a repository separate from both the intra-transactional cache and the inter-transactional cache.
20. The method of claim 18, further comprising:
removing, from a data entry list for the copy within the intra-transactional cache, a client ID and lock entry.
21. The method of claim 18, wherein
both the intra-transactional cache and the inter-transactional cache are accessible by the application server.
22. The method of claim 18, wherein
both the intra-transactional cache and the inter-transactional cache are within the application server.
23. A method, within an application server, of modifying an intra-transactional cache, comprising:
determining whether a copy of an original first data entity, accessed in the intra-transactional cache by a first client, has been updated;
determining whether the copy is also within an inter-transactional cache;
upon a determination that the copy is within the inter-transactional cache, determining whether a lock exists for the copy within the inter-transactional cache; and
removing, from the intra-transactional cache, the copy.
24. The method of claim 23, further comprising:
upon a determination that the copy has been updated,
changing a version identifier associated with the original first data entity,
generating a raw data object based upon the updated copy, and
writing the raw data object to a repository separate from both the intra-transactional cache and the inter-transactional cache.
25. The method of claim 23, further comprising:
upon a determination that no lock exists for the copy within the inter-transactional cache, updating the copy within the inter-transactional cache.
26. The method of claim 23, wherein
both the intra-transactional cache and the inter-transactional cache are accessible by the application server.
27. The method of claim 23, further comprising:
removing, from a data entry list for the copy within the intra-transactional cache, a client ID and lock entry.
28. A computer program product comprising computer usable storage medium having stored therein computer usable program code for modifying an intra-transactional cache, the computer usable program code, which when executed by an application server, causes the application server to perform:
determining whether a copy of an original first data entity, accessed in the intra-transactional cache by a first client, has been updated;
determining whether the copy is also within an inter-transactional cache;
upon the copy not being within the inter-transactional cache, adding the copy to the inter-transactional cache; and
after the copy has been added to the inter-transactional cache, removing, from the intra-transactional cache, the copy.
29. The computer program product of claim 28, wherein the computer usable program code further causes the application server to perform:
upon a determination that the copy has been updated,
changing a version identifier associated with the original first data entity,
generating a raw data object based upon the updated copy, and
writing the raw data object to a repository separate from both the intra-transactional cache and the inter-transactional cache.
30. The computer program product of claim 28, wherein the computer usable program code further causes the application server to perform:
removing, from a data entry list for the copy within the intra-transactional cache, a client ID and lock entry.
31. The computer program product of claim 28, wherein
both the intra-transactional cache and the inter-transactional cache are accessible by the application server.
32. The computer program product of claim 28, wherein
both the intra-transactional cache and the inter-transactional cache are within the application server.
33. A computer program product comprising computer usable storage medium having stored therein computer usable program code for modifying an intra-transactional cache, the computer usable program code, which when executed by an application server, causes the application server to perform:
determining whether a copy of an original first data entity, accessed in the intra-transactional cache by a first client, has been updated;
determining whether the copy is also within an inter-transactional cache;
upon a determination that the copy is within the inter-transactional cache, determining whether a lock exists for the copy within the inter-transactional cache; and
removing, from the intra-transactional cache, the copy.
34. The computer program product of claim 33, wherein the computer usable program code further causes the application server to perform:
upon a determination that the copy has been updated,
changing a version identifier associated with the original first data entity,
generating a raw data object based upon the updated copy, and
writing the raw data object to a repository separate from both the intra-transactional cache and the inter-transactional cache.
35. The computer program product of claim 33, wherein the computer usable program code further causes the application server to perform:
upon a determination that no lock exists for the copy within the inter-transactional cache, updating the copy within the inter-transactional cache.
36. The computer program product of claim 33, wherein
both the intra-transactional cache and the inter-transactional cache are accessible by the application server.
37. The computer program product of claim 33, wherein the computer usable program code further causes the application server to perform:
removing, from a data entry list for the copy within the intra-transactional cache, a client ID and lock entry.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

We claim:

1. A method of producing virus from a culture of cells, comprising the steps of:
(a) providing a culture of cells which has been infected by the virus;
(b) extracting the virus from the cells by adding a detergent to the culture and incubating for a period of time to result in a cell lysate;
(c) removing cell debris; and
(d) collecting the virus.
2. The method of claim 1 wherein the cell debris is removed by filtration.
3. The method of claim 1 wherein the cell debris is removed by step-wise filtration comprising:
(1) filtering through a prefilter having a pore size of 5 M or 8 M, and
(2) filtering after step (1) through a combination filter having pore sizes of 3 M and 0.8 M.
4. The method of claim 1 further comprising treating the cell lysate with benzonase.
5. The method of claim 2 further comprising concentrating the filtrate.
6. The method of claim 5 wherein the filtrate is concentrated by diafiltration.
7. The method of claim 1 wherein the virus is a non-enveloped virus.
8. The method of claim 1 wherein the virus is a reovirus.
9. The method of claim 8 wherein the reovirus is a mammalian reovirus.
10. The method of claim 9 wherein the mammalian reovirus is a human reovirus.
11. The method of claim 10 wherein the human reovirus is a serotype 3 virus.
12. The method of claim 11 wherein the serotype 3 reovirus is the Dearing strain.
13. The method of claim 1 wherein the reovirus is a recombinant reovirus.
14. The method of claim 1 wherein the cells are human embryo kidney 293 (HEK 293) cells.
15. The method of claim 14 wherein the HEK 293 cells are grown in suspension.
16. The method of claim 1 further comprising purifying the virus by anion exchange chromatography.
17. The method of claim 1 further comprising purifying the virus by a combination of ion exchange and size exclusion chromatography
18. A composition comprising the virus collected according to claim 1.
19. The composition of claim 18 which is suitable for clinical administration.
20. The composition of claim 19 further comprising a pharmaceutically acceptable excipient andor carrier.
21. A method of producing infectious reovirus, comprising:
(a) providing a culture of HEK 293 cells which has been infected by reovirus;
(b) extracting the virus from the cells by adding Triton X-100 to the culture and incubating at about 25 C. to about 37 C.;
(c) treating the mixture from step (b) with benzonase;
(d) removing cell debris by filtration;
(e) concentrating the filtrate by ultrafiltration or diafiltration;
(f) purifying the reovirus by a combination of ion exchange and size exclusion chromatography; and
(g) collecting the reovirus.
22. A composition comprising the reovirus prepared according to the method of claim 21.
23. The composition of claim 22 further comprising a pharmaceutically acceptable excipient andor carrier.