Author: claimsbot

1. A method of expanding a pool of pluripotent stem cells, the method comprising contacting the pluripotent stem cells with at least one agent that inhibits Dis3l2, in an amount effective to prevent differentiation of at least a portion of the pluripotent stem cells.
2. The method of claim 1, wherein the level of 3\u2032 uridylated pre-let-7 microRNA transcript in the pool of pluripotent stem cells is increased as compared to a control level of 3\u2032 uridylated pre-let-7 microRNA transcript.
3. The method of claim 1, wherein the method is performed in vitro.
4. The method of any one of claim 1, wherein the levels of expression of Sox2, Nanog andor Oct are increased in the pool of pluripotent stem cells as compared to control levels of expression of Sox2, Nanog andor Oct, respectively.
5. The method of claim 1, wherein the pluripotent stem cells display increased rates of proliferation as compared to control rates of proliferation.
6. The method of claim 1, wherein the at least one agent that inhibits Dis3l2 is an agent that inhibits gene expression of Dis3l2.
7. The method of claim 6, wherein the at least one agent that inhibits gene expression of Dis3l2 is an RNA interference (RNAi) molecule.
8. The method of claim 7, wherein the RNAi molecule is a short hairpin RNA (shRNA) or a short interfering RNA (siRNA).
9. The method of claim 1, wherein the inhibitor of Dis3l2 is an antisense nucleic acid.
10. The method of claim 1, wherein the at least one agent that inhibits Dis3l2 is an agent that inhibits Dis3l2 exonuclease activity.
11. A method of promoting degradation of uridylated RNA, the method comprising contacting uridylated RNA with an amount of Dis3l2 effective to promote degradation of uridylated RNA.
12. The method of claim 11, wherein the uridylated RNA is 3\u2032 uridylated pre-let 7 microRNA.
13. The method of claim 12, wherein the method is performed in vitro.
14. A method of stabilizing pre-let-7 microRNA in cells, the method comprising contacting cells containing pre-let-7 microRNAwith at least one agent that inhibits Dis3l2, in an amount effective to stabilize pre-let-7 microRNA.
15. The method of claim 14, wherein the level of 3\u2032 uridylated pre-let-7 microRNC transcript in the cells is increased as compared to a control level of 3\u2032 uridylated pre-let-7 microRNC transcript.
16. The method of claim 14, wherein the method is performed in vitro.
17. The method of claim 14, wherein the at least one agent that inhibits Dis3l2 is an agent that inhibits gene expression of Dis3l2.
18. The method of claim 17, wherein the at least one agent that inhibits gene expression of Dis3l2 is an RNA interference (RNAi) molecule.
19. The method of claim 18, wherein the RNAi molecule is a short hairpin RNA (shRNA) or a short interfering RNA (siRNA).
20. The method of claim 14, wherein the inhibitor of Dis3l2 is an antisense nucleic acid.
21. The method of claim 14, wherein the at least one agent that inhibits Dis3l2 is an agent that inhibits Dis3l2 exonuclease activity.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of providing electronic document services in a computer network comprising a document management entity operable to provide electronic documents to users over electronic document delivery paths between the document management entity and document users, the method comprising:
providing a document management entity remote from the users’ servers and connected to the users’ servers through the Internet;
providing a database associated with the document management entity, the database having user profiles associated with one or more of the users;
storing an electronic document in the document management entity, the stored electronic document addressed to at least one of the one or more users and transmitted across the Internet from a sending server at a source of the electronic document;
determining an IP address of the source for the stored electronic document;
examining at least one of the electronic document content and the electronic document source; and
processing the electronic document and determining options for providing access to or delivering the processed electronic document to the one or more users, both based on the examination of the at least one of the electronic document content and the electronic document source, and further in accordance with the user profile of the one or more users.
2. A method according to claim 1, wherein the processing further comprises notifying the one or more users of the storing of the electronic document in the document management entity.
3. A method according to claim 1, wherein the processing further comprises providing access to the electronic document to the one or more users via the document management entity.
4. A method according to claim 3, wherein providing access further comprises providing access via the Internet.
5. A method according to claim 1, wherein the processing further comprises delivering the entire electronic document to the one or more users.
6. A method according to claim 1, wherein the processing further comprises delivering a hyperlink to the stored electronic document to the one or more users.
7. A method according to claim 1, wherein the processing comprises processing the electronic document source according to rules in the user profile of the one or more users to whom the electronic document is addressed that are established by the one or more users.
8. A method according to claim 1, wherein the examining comprises examining the at least one of the electronic document content and electronic document source according to rules in the user profile.
9. A method according to claim 1, wherein the processing further comprises delivering different portions or versions of the electronic document to the one or more users.
10. A method according to claim 1, wherein the processing further comprises identifying a media part of the stored electronic document, modifying the electronic document by substituting different electronic content for the media part, and delivering the modified electronic document to the one or more users.
11. A method according to claim 10, wherein the different electronic content is derived from the media part.
12. A method according to claim 10, wherein the different electronic content is descriptive of the media part.
13. A method according to claim 1, wherein the processing further comprises formatting at least a portion of the electronic document in accordance with a receiving device of the one or more users, and providing the at least a portion of the electronic document to that receiving device.
14. A method according to claim 1, wherein the document management entity creates the user profile.
15. A method according to claim 1, wherein the user profile is modifiable by a user who is an intended recipient of the stored electronic document.
16. A method according to claim 1, wherein the examining further comprises filtering the stored electronic document, and wherein the processing further comprises storing filtered documents determined to be malicious in a quarantine area.
17. A method according to claim 16, further comprising sending a notification to the one or more users regarding the storing of the malicious electronic document in the quarantine area.
18. An electronic document system for managing electronic documents in a computer network, the system comprising:
a document management entity operable to provide electronic documents to users over electronic document delivery paths between the document management entity and document users, the document management entity remote from the users’ servers and connected to the users’ servers through the Internet;
a database associated with the document management entity, the database having user profiles associated with one or more of the users and configured to store in the document management entity an electronic document addressed to at least one of the one or more users and transmitted across the Internet from a sending server at a source of the electronic document; and
wherein the document management entity comprises an electronic document processor configured to:
determine an IP address of the source for the stored electronic document,
examine at least one of the electronic document content and the electronic document source, and
process the electronic document and determine options for providing access to or delivering the processed electronic document to the one or more users, both based on the examination of the at least one the electronic document content and the electronic document source, and further in accordance with the user profile of the one or more users.
19. A system according to claim 18, wherein the electronic document processor is further configured to notify the one or more users of the storing of the electronic document in the document management entity.
20. A system according to claim 18, wherein the electronic document processor is further configured to provide access to the electronic document to the one or more users via the document management entity.
21. A system according to claim 20, wherein providing access comprises providing access via the Internet.
22. A system according to claim 18, wherein the electronic document processor is further configured to deliver the entire electronic document to the one or more users.
23. A system according to claim 18, wherein the electronic document processor is further configured to deliver a hyperlink to the stored electronic document to the one or more users.
24. A system according to claim 18, wherein the electronic document processor is further configured to process the electronic document source according to rules in the user profile of the one or more users to whom the electronic document is addressed that are established by the one or more users.
25. A system according to claim 18, wherein the processing by the electronic document processor further comprises examining the at least one of the electronic document content and electronic document source according to rules in the user profile.
26. A system according to claim 18, wherein the processing by the electronic document processor further comprises delivering different portions or versions of the electronic document to the one or more users.
27. A system according to claim 18, wherein the processing by the electronic document processor further comprises identifying a media part of the stored electronic document, modifying the electronic document by substituting different electronic content for the media part, and delivering the modified electronic document to the one or more users.
28. A system according to claim 27, wherein the different electronic content is derived from the media part.
29. A system according to claim 27, wherein the different electronic content is descriptive of the media part.
30. A system according to claim 18, wherein the processing by the electronic document processor further comprises formatting at least a portion of the electronic document in accordance with a receiving device of the one or more users, and providing the at least a portion of the electronic document to that receiving device.
31. A system according to claim 18, wherein the document management entity creates the user profile.
32. A system according to claim 18, wherein the user profile is modifiable by a user who is an intended recipient of the stored electronic document.
33. A system according to claim 18, wherein the examining by the electronic document processor further comprises filtering the stored electronic document, and wherein the processing by the electronic document processor further comprises storing filtered documents determined to be malicious in a quarantine area.
34. A system according to claim 33, wherein the electronic document processor is further configured to send a notification to the one or more users regarding the storing of the malicious electronic document in the quarantine area.
35. A method of providing electronic document services in a computer network comprising a document management entity operable to provide electronic documents to users over electronic document delivery paths between the document management entity and document users, the method comprising:
providing a document management entity remote from the users’ servers and connected to the users’ servers through the Internet;
providing a database associated with the document management entity, the database having user profiles associated with one or more of the users;
storing an electronic document in the document management entity, the stored electronic document addressed to at least one of the one or more users and transmitted across the Internet from a sending server at a source of the electronic document;
determining an IP address of the source for the stored electronic document;
examining at least one of the electronic document content and the electronic document source;
processing the electronic document based on the examination of the at least one the electronic document content and the electronic document source, and further in accordance with the user profile of the one or more users to whom the document is addressed;
delivering a hyperlink to the stored electronic document to the one or more users;
providing access via the document management entity to the electronic document to the one or more users that click on the hyperlink; and
determining options for providing access to or delivering the entire electronic document to the one or more users that clicked on the hyperlink also based on the examination of the at least one the electronic document content and the electronic document source, and further in accordance with the user profile of the one or more users.
36. A method according to claim 35, wherein providing access further comprises providing access via the Internet.
37. A method according to claim 35, wherein the processing comprises processing the electronic document source according to rules in the user profile of the one or more users to whom the electronic document is addressed that are established by the one or more users.
38. A method according to claim 35, wherein the examining comprises examining the at least one of the electronic document content and electronic document source according to rules in the user profile.
39. A method according to claim 35, wherein the processing further comprises formatting at least a portion of the electronic document in accordance with a receiving device of the one or more users, and providing the at least a portion of the electronic document to that receiving device.
40. A method according to claim 35, wherein the examining further comprises filtering the stored electronic document, and wherein the processing further comprises storing filtered documents determined to be malicious in a quarantine area.

1. A method for producing a clotting factor concentrate without using chromatography comprising the steps of:
adding trisodium citrate to plasma to yield a concentration of trisodium citrate of between about 10% weight by volume and about 15% weight by volume;
allowing cryoprecipitate to form in the plasma;
separating the cryoprecipitate from the plasma;
extracting in the cold, below 10 C. the cryoprecipitate with an extraction solution containing calcium chloride; and
separating the cryoprecipitate from the extraction solution wherein the separated extraction solution is the clotting factor concentrate.
2. The method according to claim 1, wherein forming cryoprecipitate does not involve freezing the plasma.
3. The method according to claim 1, wherein the step of separating the cryoprecipitate from the plasma employs centrifugation.
4. The method according claim 1 further comprising the step of removing citrate from the clotting factor concentrate.
5. The method according claim 1 further comprising the step of removing calcium from the clotting factor concentrate.
6. The method according to claim 1, wherein the step of separating the cryoprecipitate from the extraction solution employs centrifugation.
7. The method according to claim 1, wherein the extraction solution contains about 0.9% sodium chloride, weight by volume and about 0.3M calcium chloride.
8. The method according to claim 1, wherein the extraction solution has a pH between about 5.0 and about 7.0.
9. The method according to claim 1, wherein the step of adding trisodium citrate is accomplished by collecting the plasma directly into a container holding the trisodium citrate.
10. A method for producing a clotting factor concentrate without using chromatography comprising the steps of:
adding trisodium citrate to plasma to yield a concentration of trisodium citrate of about 12% weight by volume;
allowing cryoprecipitate to form in the plasma;
separating the cryoprecipitate from the plasma by centrifugation;
extracting in the cold, below 10 C. the cryoprecipitate with an extraction solution comprising a cold solution containing sodium chloride and calcium chloride; and
separating the cryoprecipitate from the extraction solution by centrifugation wherein the separated extraction solution is the clotting factor concentrate.
11. The method according to claim 10, wherein forming cryoprecipitate does not involve freezing the plasma.
12. The method according claim 10 further comprising the step of removing citrate from the clotting factor concentrate.
13. The method according claim 10 further comprising the step of removing calcium from the clotting factor concentrate.
14. The method according to claim 10 , wherein the extraction solution contains about 0.9% sodium chloride, weight by volume and about 0.3M calcium chloride.
15. The method according to claim 10 , wherein the extraction solution has a pH between about 5.0 and about 7.0.
16. The method according to claim 10 , wherein the extraction solution has a pH of about 5.5.
17. The method according to claim 10 , wherein the step of adding trisodium citrate is accomplished by collecting the plasma directly into a container holding the trisodium citrate.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An apparatus for providing power and network signal from a powerline network, comprising:
a power component configured to electrically connect to a powerline network;
a power unit electrically connected to said power component;
a connecting port configured to electrically connect to a network apparatus;
a power over Ethernet module electrically connected to said power unit and said connecting port, to transform a power from said power unit into a network power; and
a network communication module electrically connected to said power component and said power unit, to access a network signal from said power component and transmit said network signal to said connecting port.
2. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said network communication module comprises:
a powerline-signal coupling unit electrically connected to said power component;
a signal processing unit electrically connected to said powerline-signal coupling unit, to access and process said network signal; and
an Ethernet access unit electrically connected to said signal processing unit, to receive said network signal and transmit said network signal to said power over Ethernet module, and to receive an apparatus signal from said network apparatus and transmit said apparatus signal to said signal processing unit, wherein said power over Ethernet module is used to integrate said network signal with said network power, which is then transmitted to said connecting port.
3. An apparatus for providing power and network signal from a powerline network according to claim 2, wherein said power over Ethernet module comprises:
a power over Ethernet unit electrically connected to said power unit to obtain said power from said power unit; and
a power over Ethernet transformer electrically connected to said power over Ethernet unit and said network communication module, and used to integrate said network signal with said network power.
4. An apparatus for providing power and network signal from a powerline network according to claim 2, wherein said powerline-signal coupling unit is a coupling transformer.
5. An apparatus for providing power and network signal from a powerline network according to claim 2, wherein said network communication module comprises:
a powerline-signal coupling unit electrically connected to said power component;
a signal processing unit electrically connected to said powerline-signal coupling unit, to access and process said network signal;
an Ethernet transformer electrically connected to said connecting port; and
an Ethernet access unit electrically connected to said signal processing unit and said Ethernet transformer, to receive said network signal and transmit said network signal to said connecting port through said Ethernet transformer, and to receive an apparatus signal from said connecting port and transmit said apparatus signal through said Ethernet transformer to said signal processing unit.
6. An apparatus for providing power and network signal from a powerline network according to claim 5, wherein said power over Ethernet module comprises a power over Ethernet unit, which electrically connects to said power unit and said connecting port, to transform said power from said power unit into said network power and then to transmit said network power to said connecting port.
7. An apparatus for providing power and network signal from a powerline network according to claim 5, wherein said powerline-signal coupling unit is a coupling transformer.
8. An apparatus for providing power and network signal from a powerline network according to claim 1, further comprising a shell which integrates and contains said power unit, said connecting port, said power over Ethernet module and said network communication module.
9. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said power component is a power cord with a plug on one end of said power cord, and said plug is put into a socket to electrically connect to said powerline network.
10. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said power component is an electric plug which is put into a socket to electrically connect to said powerline network.
11. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said connecting port is RJ45 port, which comprises a data receiving pair and a data transmitting pair.
12. An apparatus for providing power and network signal from a powerline network according to claim 11, wherein said power over Ethernet module comprises:
a power over Ethernet unit electrically connected to said power unit to obtain said power from said power unit; and
a power over Ethernet transformer electrically connected to said power over Ethernet unit and said network communication module, to provide said network power.
13. An apparatus for providing power and network signal from a powerline network according to claim 11, wherein said RJ45 further comprise at least one spare pairs.
14. A n apparatus for providing power and network signal from a powerline network according to claim 13, wherein said power over Ethernet module comprises a power over Ethernet unit electrically connected to said power unit and said RJ45 port, to transform said power from said power unit into said network power which is then transmitted by said spare pairs of said RJ45 port.
15. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said network power is direct voltage.
16. An apparatus for providing power and network signal from a powerline network according to claim 1, wherein said connecting port is any connector which comprises a data receiving pair, a data transmitting pair and direct voltage.