All The Claims

What is claimed is:

1. A method for formulating a protein comprising:
combining a protein mixture with a cross-linking reagent;
placing the protein mixture into a mold after combining the protein mixture with a cross-linking reagent;
hardening the protein mixture; and
removing the hardened protein mixture from the mold.
2. A method for formulating a protein according to claim 1, wherein hardening the protein mixture comprises exposing the protein mixture to a non-liquid cross-linking process.
3. A method for formulating a protein according to claim 2, wherein hardening the protein mixture further comprises incubating the protein mixture prior to exposing the protein mixture to the non-liquid cross-linking process.
4. A method for formulating a protein according to claim 2, wherein hardening the protein mixture further comprises immersing the protein mixture in a cross-linking solution after exposing the protein solution to the non-liquid cross-linking process.
5. A method for formulating a protein according to claim 2, wherein exposing the protein mixture to a non-liquid cross-linking process comprises exposing the protein mixture to a vapor phase that is approximately 12.5% (ww) glutaraldehyde for approximately 16 hours.
6. A method for formulating a protein according to claim 3, wherein incubating the protein mixture comprises maintaining the protein mixture at approximately room temperature for approximately two hours.
7. A method for formulating a protein according to claim 4, wherein immersing the protein mixture in a cross-linking solution comprises submerging the protein mixture in a buffered solution that is approximately 2.5% (ww) glutaraldehyde for approximately one hour.
8. A method for formulating a protein according to claim 1, wherein the protein mixture comprises glucose oxidase and human serum albumin.
9. A method for formulating a protein according to claim 1, wherein the cross-linking reagent is glutaraldehyde.
10. A method for formulating a protein according to claim 8, wherein the cross-linking reagent is glutaraldehyde.
11. A method for formulating a protein according to claim 1, wherein the cross-linking reagent is selected from a group consisting of glutaraldehyde, disuccinimidyl suberate (DSS), and 1-Ethyl-3 (3-Dimethylaminopropyl) Carbodiimide (EDC).
12. A method for formulating a protein according to claim 8, wherein the glucose oxidase has a concentration that is between approximately 67,000 Uml and 150,000 Uml.
13. A method for formulating a protein according to claim 9, wherein the human serum albumin has a concentration that is between approximately 23% (wv) and 32.5% (wv).
14. A method for formulating a protein according to claim 12, wherein the human serum albumin has a concentration that is between approximately 23% (wv) and 32.5% (wv).
15. A method for formulating a protein according to claim 1, wherein the mold is configured to form the protein mixture into at least one elongated rope-like structure.
16. A method for formulating a protein according to claim 15, wherein the method further comprises cutting the at least one elongated rope-like structure into pieces.
17. A method for formulating a protein according to claim 15, wherein the elongated rope-like structure is semi-cylindrical.
18. A method for formulating a protein according to claim 1, wherein the mold comprises:
a block with a surface wherein the surface has at least one recess in it; and
wherein the protein mixture is placed in the recess when it is placed into the mold.
19. A method for formulating a protein according to claim 18, wherein the recess comprises at least one channel.
20. A method for formulating a protein according to claim 19, wherein the recess is semi-cylindrical.
21. A method for formulating a protein according to claim 19, wherein the recess comprises multiple intersecting channels.
22. A method for formulating a protein according to claim 1, wherein the method further comprises adding silicone to the protein mixture.
23. A method for formulating a protein according to claim 22, wherein adding silicone to the protein solution comprises:
obtaining silicone particles; and
adding the silicone particles to the protein mixture.
24. A method for formulating a protein according to claim 23, wherein the volume of the silicone particles is less than 20% of the volume of the protein mixture.
25. A method for formulating a protein according to claim 23, wherein mixing the silicone particles into the protein mixture occurs prior to the protein mixture being placed into the mold.
26. A method for formulating a protein according to claim 1, wherein the method further comprises washing the protein mixture after the completion of hardening the protein mixture.
27. A method for formulating a protein according to claim 1, wherein the method further comprises cutting the protein mixture into at least two pieces after removing the protein mixture from the mold.
28. A sensor comprising:
a sensor body; and
an active protein disposed within the sensor body, the active protein comprising gluscose oxidase, human serum albumin, and a cross-linking reagent.
29. A sensor according to claim 28, wherein the active protein is hardened before it is disposed within the sensor body.
30. A sensor according to claim 28, wherein the cross-linking reagent is selected from a group consisting of glutaraldehyde, disuccinimidyl suberate (DSS), and 1-Ethyl-3 (3-Dimethylaminopropyl) Carbodiimide (EDC).
31. A protein matrix formed according to the method of claim 1.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A color perception tester, comprising:
a color matching processor that executes a color matching processing on a color monitor;
an image display controller that executes at least one display step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done;
whereby said image display controller changes display time of each image for color perception test so as to become gradually longer as a first element, or changes one element of five elements, a hue, a brightness, a saturation of each image for color perception test, and a brightness of a background or a stimulus if said image for color perception test is comprised of said background image and said stimulus shown in said background image as said first element in such a state that said display time is fixed when displaying said two or more images for color perception test in said one display step.
2. The color perception tester according to claim 1, wherein said image display controller executes at least two display steps, a first display step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done, and a second display step wherein two or more images for color perception test are displayed in order on said color monitor, and
said image display controller changes said first element when displaying each image for color perception test in each display step, and changes a second element, one element excluding said first element of six elements, hue, brightness, saturation and display time of each image for color perception test and brightness of a background or a stimulus if said image for color perception test is comprised of said background image and said stimulus shown in said background image gradually in said first display step and said second display step and fixes four elements excluding said first element and said second element during said first display step and said second display step.
3. The color perception tester according to claim 1 wherein said image display controller has a display time controller that controls display time of each image for color perception test.
4. The color perception tester according to claim of 1 further comprising a test result display that displays an image for color perception test, information on color combination used in displayed image for color perception test, a result whether or not an examinee can perceive said image for color perception test and display time of said image for color perception test.
5. The color perception tester according to claims 1 further comprising an image data storage that stores two or more images for color perception test, wherein said image display controller displays said image for color perception test stored in said image data storage.
6. The color perception tester according to claims 1 further comprising an image composer that composes said image for color perception test to be displayed, wherein said image display controller displays said image for color perception test that was composed through said image composer.
7. The color perception tester according to claim 6, further comprising an image data storage that stores two or more images for color perception test, wherein said image for color perception test composed through said image composer is temporally stored in said image data storage.
8. The color perception tester according to claims 1 wherein said image display controller displays an image in gray color on said color monitor for a predetermined time during switching said image for color perception test.
9. The color perception tester according to claims 1 further comprising a statistical data computer that computes test results of a color perception test done on two or more examinees comprising an examinee group, as statistical data, an accumulated data storage that stores two or more statistical data obtained from said examinee group as accumulated data, and a data comparator that compares said test result of said color perception test done on at least one of said examinees with said accumulated data.
10. Control program for controlling a computer, for providing said computer to function as at least one member of the group comprising:
a color matching processor that executes a color matching processing on a color monitor; and
an image display controller that executes at least one display step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done;
whereby said image display controller changes display time of each image for color perception test so as to become gradually longer as a first element, or changes one element of five elements, a hue, a brightness, a saturation of each image for color perception test, and a brightness of a background or a stimulus if said image for color perception test is comprised of said background image and said stimulus shown in said background image as the first element in such a state that said display time is fixed when displaying said two or more images for color perception test in said one display step.
11. The control program according to claim 10, wherein said image display controller executes at least two display steps, a first step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done, and a second display step wherein two or more images for color perception test are displayed in order on said color monitor; and said image display controller changes said first element when displaying each image for color perception test in each display step, and gradually changes said second element that is one element excluding said first element of six elements, a hue, a brightness, a saturation, a display time of each image for color perception test, and a brightness of a background or a stimulus if said image for color perception test is comprised of said background image and said stimulus shown in said background image in said first display step and said second display step, and fixes four elements excluding said first element and said second element in said first display step and said second display step.
12. The control program according to claim 10 or 11, for providing said computer to function as at least one member of the group comprising:
a statistical data computer that computes test result of a color perception test done on two or more examinees comprising an examinee group, as statistical data, an accumulated data storage that stores two or more statistical data obtained from said examinee group as accumulated data; and
a data comparator that computes said test result of said color perception test done on at least one examinee with said accumulated data.
13. A method of controlling a color perception tester, comprising:
a step of executing a color matching processing on a color monitor through a color matching processor;
a step of executing at least one display step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done through said image display controller;
whereby said image display controller changes display time of each image for color perception test so as to become gradually longer as a first element, or changes one element of five elements, a hue, a brightness, a saturation of each image for color perception test, and a brightness of a background or a stimulus if said image for color perception test is comprised of said background image and said stimulus showing in said background image as the first element in such a state that said display time is fixed when displaying said two or more images for color perception test in said one display step.
14. The method of controlling the color perception tester according to claim 13, wherein said image display controller executes at least two display steps, a first display step wherein two or more images for color perception test are displayed in order on said color monitor on which said color matching processing was done, and a second display step wherein two or more images for color perception test are displayed in order on said color monitor, and said image display controller changes said first element when displaying each image for color perception test in each display step, and changes a second element, one element excluding said first element of a six elements, a hue, a brightness, a saturation and a display time of each image for color perception test and a brightness of a background or a stimulus if aid image for color perception test is comprised of said background image and said stimulus shown in said background image gradually in said first display step and said second display step and fixes four elements excluding said first element and said second element during said first display step and said second display step.
15. The method of controlling the color perception tester according to claim 13 or 14, further comprising:
a step of computing test result of a color perception test done on two or more examinees comprising an examinee group as statistical data through a statistical data computer,
a step of storing two or more statistical data obtained from said examinee group as accumulated data through an accumulated data storage; and a step of comparing said test result of said color perception test done on at least one examinee with said accumulated data through a data comparator.
16. The color perception tester according to claims 1 wherein said image for color perception test is one showing figures, comprising characters, numerals and Landolt rings, in a background.
17. The control program according to claims 10 wherein said image for color perception test is one showing figures, comprising characters, numerals and Landolt rings, in a background.
18. The method of controlling the color perception tester according to claims 13 wherein said image for color perception test is one showing figures, comprising characters, numerals and Landolt rings, in a background.
19. The color perception tester according to claims 1, wherein a user is capable of selecting to change display time of said image for color perception test as said first element, to change hue of said image for color perception test as said first element, to change brightness of said image for color perception test as said first element, to change saturation of said image for color perception test as said first element, or to change brightness of said background or said stimulus as said first element.
20. The control program according to claims 10 wherein a user is capable of selecting to change display time of said image for color perception test as said first element, to change hue of said image for color perception test as said first element, to change brightness of said image for color perception test as said first element, to change saturation of said image for color perception test as said first element, or to change brightness of said background or said stimulus as said first element.
21. The method of controlling color perception tester according to claims 13 wherein a user is capable of selecting to change display time of said image for color perception test as said first element, to change hue of said image for color perception test as said first element, to change brightness of said image for color perception test as said first element, to change saturation of said image for color perception test as said first element, or to change brightness of said background or said stimulus as said first element.

1. A method for controlling a logical switching element comprising one or more logical ports, the logical switching element implemented in one or more managed switching elements that forward data packets in a network, the method comprising:
specifying a set of network addresses for a logical port of the logical switching element; and
dropping a data packet entering or exiting the logical switching element through the logical port when the data packet does not include a network address in the specified set of network addresses.
2. The method of claim 1, wherein the set of network addresses is specified in an access control list (ACL) for the logical port.
3. The method of claim 1, wherein the set of network addresses includes at least one of a Media Access Control (MAC) address and an Internet Protocol (IP) address.
4. The method of claim 3, wherein the data packet includes a source MAC address, wherein dropping the data packet comprises dropping the data packet when the set of network addresses does not include the source MAC address and the data packet attempts to enter the logical switching element through the logical port.
5. The method of claim 3, wherein the data packet includes a source IP address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the source IP address and the data packet attempts to enter the logical switching element through the logical port.
6. The method of claim 3, wherein the data packet includes a destination MAC address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the destination MAC address and the data packet attempts to exit the logical switching element through the logical port.
7. The method of claim 3, wherein the data packet includes a destination IP address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the destination IP address and the data packet attempts to exit the logical switching element through the logical port.
8. The method of claim 3, wherein the data packet includes an Access Resolution Protocol (ARP) response that includes a MAC address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the ARP response’s MAC address and the data packet attempts to enter the logical switching element through the logical port.
9. The method of claim 3, wherein the data packet includes an Access Resolution Protocol (ARP) response that includes an IP address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the APR response’s IP address and the data packet attempts to enter the logical switching element through the logical port.
10. The method of claim 1 further comprising receiving inputs from a user of the logical switching element that include the set of network addresses.
11. The method of claim 1, wherein the logical port is for assigning to a device from which the logical switching element receives data packets.
12. A method of implementing a logical switching element on a set of managed switching elements, the method comprising:
providing a first set of tables for storing a set of network addresses;
providing a second set of tables for storing information for a logical port of a logical switching elements; and
mapping the set of network addresses to the information for the logical port by performing a set of database join operations on the set of network addresses stored in the first set of tables,
wherein the logical switching elements drops a data packet entering or exiting the logical switching element through the logical port when the data packet does not include a network address in the set of network addresses.
13. The method of claim 12, wherein said mapping is performed to generate an access control list (ACL) for the logical switching element.
14. The method of claim 12, wherein the data packet includes a MAC address.
15. The method of claim 12, wherein the data packet includes an IP address.
16. A logical switching element implemented in a set of managed switching elements that forward data in a network, the logical switching element comprising:
a logical port for receiving data into the logical switching element; and
an access control list (ACL) for storing a set of network addresses,
wherein the logical switching elements drops a data packet entering or exiting the logical switching element through the logical port when the data packet does not include a network address in the set of network addresses.
17. The logical switching element of 16, wherein the set of network addresses includes at least one of a Media Access Control (MAC) address and an Internet Protocol (IP) address.
18. The logical switching element of 16, wherein the data packet includes a source network address, wherein dropping the data packet comprises dropping the data packet when the set of network addresses does not include the source network address and the data packet attempts to enter the logical switching element through the logical port.
19. The logical switching element of 16, wherein the data packet includes a destination network address, wherein dropping the data packet comprises dropping the data packet when the set of network addresses does not include the destination network address and the data packet attempts to exit the logical switching element through the logical port.
20. The logical switching element of 16, wherein the data packet includes an Access Resolution Protocol (ARP) response that includes a network address, wherein dropping the data packet comprises dropping the data packet when the set of network address does not include the ARP response’s network address.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of augmenting expression of a heterologous nucleic acid in a eukaryotic cell comprising decreasing expression or activity of endogenous Interferon-induced protein-16 (IFI16).
2. The method of claim 1, wherein said heterologous nucleic acid comprises a coding sequence.
3. The method of claim 2, wherein said heterologous nucleic acid coding sequence comprises non-viral DNA.
4. The method of claim 2, wherein said heterologous nucleic acid coding sequence encodes a mammalian gene product.
5. The method of claim 1, wherein said heterologous nucleic acid comprises vectored DNA.
6. The method of claim 1, wherein an IFI16 protein is encoded by a nucleic acid comprising the sequence of SEQ ID NO:5.
7. The method of claim 1, wherein an IFI16 protein comprises the amino acid sequence of SEQ ID NO:1, 2, 3, or 4.
8. The method of claim 1, wherein said expression is decreased by contacting said cell with an IFI16-specific siRNA.
9. The method of claim 8, wherein said IFI16-specific siRNA is selected from the group consisting of GAUCUGUAAUUCAUAGUCA (SEQ IDNO: 6), GGACCAGCCCUAUCAAGAA (SEQ IDNO: 7), GGAGUAAGGUGUCCGAGGA (SEQ IDNO: 8), CAGCGUAACUCCUAAAAUC (SEQ IDNO: 9), and GCUGGUCCUAACCAAACGU (SEQ IDNO: 10).
10. The method of claim 1, wherein said expression is decreased by contacting said cell with an IFI16-specific shRNA.
11. The method of claim 10, wherein said IFI16-specific shRNA is selected from the group consisting of
#1\u2003Sense:\u2003
(SEQ\u2003ID\u2003NO:\u200311)
CCACAAUCUACGAAAUUCA,\u2003

Anti-sense:
(SEQ\u2003ID\u2003NO:\u200312)
UGAAUUUCGUAGAUUGUGG;

#2\u2003Sense:\u2003
(SEQ\u2003ID\u2003NO:\u200313)
CCAUCCAGCAGUUUCUUCA,\u2003

Anti-sense:
(SEQ\u2003ID\u2003NO:\u200314)
UGAAGAAACUGCUGGAUGG;\u2003
and

#3\u2003Sense:\u2003
(SEQ\u2003ID\u2003NO:\u200315)
GGAAGGAGAUAAACUGAAA,\u2003

Anti-sense:
(SEQ\u2003ID\u2003NO:\u200316)
UUUCAGUUUAUCUCCUUCC.
12. The method of claim 1, wherein said expression is decreased by contacting said cell with an IFI16-specific antisense nucleic acid.
13. The method of claim 1, wherein said activity is decreased by contacting said cell with an inhibitor of IFI16 binding to DNA.
14. The method of claim 13, wherein said inhibitor binds to a HIN domain of IFI16
15. The method of claim 13, wherein said inhibitor binds to residues K663, R667, K732, K734, K759,andor R764 of IFI16 isotype A or a corresponding residue of isotype B or C.
16. An expression vector comprising a heterologous coding sequence and an IFI16 regulating nucleic acid sequence.
17. The method of claim 8, wherein said IFI16-regulating nucleic acid sequence comprises an IFI16-specific shRNA.
18. A method of modulating homologous recombination of a heterologous DNA in a eukaryotic cell comprising contacting said cell with an inhibitor of IFI16 expression or activity.
19. The method of claim 10, wherein said inhibitor reduces binding of IFI16 to heterologous DNA or reduces IFI16-mediated addition of silencing chromatin to said heterologous DNA.
20. A method of modulating homologous recombination of a heterologous DNA in a eukaryotic cell comprising contacting said cell with a compound that increases IFI16 expression or activity.
21. A method of modulating lentiviral vector integration in a eukaryotic cell comprising contacting said cell with an inhibitor of IFI16 expression or activity.
22. The method of claim 21, wherein said inhibitor reduces binding of IFI16 to heterologous DNA, reduces IFI16-mediated addition of silencing chromatin to said heterologous DNA, or inhibits a conformational change of IFI16.
23. A method of modulating lentiviral vector integration in a eukaryotic cell comprising contacting said cell with a modulator of IFI16 expression or activity.
24. A method of identifying a IFI16-modulating compound, comprising providing a eukaryotic cell infected with a Herpes virus comprising a heterologous nucleic acid encoding a detectable marker, contacting said cell with a candidate compound, and measuring expression of said detectable marker, wherein a decrease or increase in expression of said marker indicates that said candidate compound modulates IFI16-inhibitory activity.
25. The method of claim 24, wherein said modulating compound inhibits IFI16 expression or activity.
26. The method of claim 24, wherein said modulating compound increases IFI16 expression or activity.
27. The method of claim 24, wherein said detectable marker comprises a green fluorescent protein (GFP), luciferase, or beta-galactosidase.
28. A method of modulating integration of a lentiviral expression vector in a eukaryotic cell, comprising decreasing expression or activity of IFI16.