1. A method of linking an antigenic, detoxified bacterial lipooligosaccharide having a lipid A region with a terminal glucosamine glycosidic phosphate group to an immunologically acceptable carrier through said lipid A region, which method comprises complete removal of said terminal glycosidic phosphate group to yield detoxified reducing bacterial lipooligosaccharide with preserved integrity and immunogenicity of core-oligosaccharide epitopes and then conjugating said detoxified reducing bacterial lipooligosaccharide to said immunologically acceptable carrier.
2. An antigenic, detoxified reducing bacterial lipooligosaccharide having a lipid A region and having preserved integrity and immunogenicity of core-oligosaccharide epitopes, linkable to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region.
3-5. (canceled)
6. A conjugate vaccine for combating a Gram-negative or other bacterium comprising an antigenic, detoxified reducing bacterial lipooligosaccharide according to claim 2 linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region.
7. The conjugate vaccine of claim 6, wherein said immunogenic carrier is a protein.
8. The conjugate vaccine of claim 7, wherein said immunogenic carrier protein is selected from the group consisting of tetanus toxintoxoid, cross-reacting material (CRM), NTHi high molecular weight protein, diphtheria toxintoxoid, detoxified P. aeruginosa toxin A, cholera toxintoxoid, pertussis toxintoxoid, Clostridium perfringens exotoxinstoxoid, hepatitis B surface antigen, hepatitis B core antigen, rotavirus VP 7 protein, respiratory syncytial virus F and G proteins.
9. The conjugate vaccine of claim 8, wherein said immunogenic carrier protein is tetanus toxoid.
10. The conjugate vaccine of claim 8, wherein said immunogenic carrier protein is CRM197.
11. A conjugate vaccine for combating a Gram-negative bacterium comprising an antigenic, detoxified reducing bacterial lipooligosaccharide according to claim 2 linked via a linker to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region.
12. The conjugate vaccine of claim 11, wherein said linker is selected from the group consisting of M2C2H, cystamine, adipic acid di-hydrazide, \u25a1-aminohexanoic acid, chlorohexanol dimethyl acetal, D-glucuronolactone and p-nitrophenylethyl amine.
13. The conjugate vaccine of claim 12, wherein said linker is M2C2H.
14. The conjugate vaccine of claim 12, wherein said linker is cystamine.
15. A pharmaceutical composition comprising the conjugate vaccine of claim 6 in association with an adjuvant.
16. The pharmaceutical composition of claim 15, wherein said adjuvant is selected from the group consisting of Freund’s adjuvant, alum and Ribi.
17. The pharmaceutical composition of claim 15 further comprising a pharmaceutically acceptable diluent.
18. A polyvalent conjugate vaccine comprising a plurality of different antigenic, detoxified reducing bacterial lipooligosaccharides according to claim 2 covalently linked to an immunogenic carrier.
19. A multivalent conjugate vaccine comprising a plurality of different conjugate vaccines according to claim 6.
20. The conjugate vaccine of claim 6 that elicits immunogenicity of inner-core oligosaccharide epitope(s), said conjugate vaccine being protective against a Neisseria meningitidis or Haemophilus influenzae infection.
21. The conjugate vaccine of claim 11 that elicits immunogenicity of inner-core oligosaccharide epitope(s), said conjugate vaccine being protective against a Neisseria meningitidis or Haemophilus influenzae infection.
22. A pharmaceutical composition comprising the conjugate vaccine of claim 11 in association with an adjuvant.
23. A multivalent conjugate vaccine comprising a plurality of different conjugate vaccines according to claim 11.
24. A method of linking an antigenic, detoxified bacterial lipooligosaccharide having a lipid A region with a terminal glucosamine glycosidic phosphate group to an immunologically acceptable carrier through said lipid A region, which method comprises ex vivo detoxification of an antigenic bacterial lipooligosaccharide followed by complete removal of said terminal glycosidic phosphate group to yield a detoxified reducing bacterial lipooligosaccharide with preserved integrity and immunogenicity of core-oligosaccharide epitopes and then conjugating said detoxified reducing bacterial lipooligosaccharide to said immunologically acceptable carrier.
25. An antigenic, detoxified reducing bacterial lipooligosaccharide having a lipid A region and having preserved integrity and immunogenicity of core-oligosaccharide epitopes, linkable to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region, with the proviso that said antigenic, detoxified reducing bacterial lipooligosaccharide has other than a ratio of about 2:3:2:1:1 of KDO:heptose:glucosamine:glucose:N-acetylglucosamine.
26. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having up to a five-fold increase in immunogenicity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
27. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having a five- to ten-fold increase in immunogenicity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
28. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having at least a ten-fold increase in immunogenicity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
29. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having at least a three-fold increase in bactericidal activity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
30. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having a 3-5-fold increase in bactericidal activity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
31. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having at least a ten-fold increase in bactericidal activity linked to an immunologically acceptable carrier through complete dephosphorylation of glycosidically-linked phosphate or phosphate substituents of the terminal glycose of the lipid A region when compared with an equivalent lipooligosaccharide linked to an immunologically acceptable carrier through 2-keto-3-deoxyoctulosonic acid.
32. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having a minimum basal glycan structure of 2:2:2:1 of KDO:heptose:glucosamine:N-acetylglucosamine.
33. The antigenic, detoxified reducing bacterial lipooligosaccharide of claim 2 having a minimum basal glycan structure of 1:3:2 of KDO:heptose:glucosamine.
34. A method of combating a Gram-negative or other bacterial infection in a mammal which method comprises administering a pharmaceutically effective amount of an antigenic, detoxified reducing bacterial lipooligosaccharide according to claim 2 in conjunction with a pharmaceutically acceptable carrier to said mammal.
35. Use of an antigenic, detoxified reducing bacterial lipooligosaccharide according to claim 2 in manufacture of a medicament for combating a Gram-negative or other bacterial infection in a mammal.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A computerized data processing method to control access to consumer devices, the method comprising:
collecting a plurality of user-specific parameter values as a user attempts to access a consumer device;
determining at least two of the plurality of user-specific parameter values that uniquely identify the user;
determining two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong;
retrieving, from a reference database, at least two user-specific reference values that belong to the two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong and that have been stored in the reference database for the user;
determining whether the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values; and
in response to determining that the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values, granting, to the user, access to the consumer device.
2.-3. (canceled)
4. The computerized data processing method of claim 1, further comprising:
ranking the plurality of user-specific parameter values collected as the user attempts to access the consumer device based on a date when the plurality of user-specific parameter values was collected to determine whether the plurality of user-specific parameter values is to be used to determine whether to grant, to the user, access to the consumer device; and
ranking the at least two user-specific reference values based on a date when the at least two user-specific reference values were stored in the reference database to determine whether the at least two user-specific reference values are to be used to compare with the plurality of user-specific parameter values; and
wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values are performed automatically or manually by an authenticator.
5. The computerized data processing method of claim 4, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a noisy environment are based on assigning high ranks to values obtained as results of a contact with a sensor, and assigning low ranks to values collected as results of an object scanning.
6. The computerized data processing method of claim 4, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a low light environment are based on assigning high ranks to values collected using a voice and speech recognition device, and assigning low ranks to values collected as results of a contact with a sensor.
7. The computerized data processing method of claim 1,
wherein the consumer device is any one of: a computerized security device, a motorized vehicle, a motorized machinery, a domestic appliance, a bank kiosk, a television set, a setup box, a play station, a movie recorder, a computer device, a portable device, a smart phone, or a digital camera;
wherein granting, to the user, access to the consumer device comprises one or more of:
positively authenticating the user with a security system of the consumer device;
determining that the user is capable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
permitting to continue operating the consumer device;
permitting to withdraw funds from a bank account using the consumer device;
permitting to deposit funds to a bank account using the consumer device;
permitting to watch a specially-rated television program on the consumer device;
permitting to record a specially-rated television program on the consumer device;
permitting to play a specially-rated program on the consumer device;
permitting to access computer resources of the consumer device; or
permitting to use functionalities of the consumer device.
8. The computerized data processing method of claim 1,
wherein the plurality of user-specific parameter values includes two or more of: user credentials, a textual-based parameter value, a digital image, a biometric parameter value, a physiological parameter value, or a behavioral parameter value.
9. The computerized data processing method of claim 1, further comprising:
in response to determining that the at least two of the plurality of user-specific parameter values do not match the at least two user-specific reference values, denying, the user, access to the consumer device;
wherein denying, the user, access to the consumer device comprises one or more of:
refusing to positively authenticate the user with a security system of the consumer device;
determining that the user is incapable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
turning off the consumer device;
preventing the user from withdrawing funds from a bank account using the consumer device;
preventing the user from depositing funds to a bank account using the consumer device;
preventing the user from watching a specially-rated television program on the consumer device;
preventing the user from recording a specially-rated television program on the consumer device;
preventing the user from playing a specially-rated program on the consumer device;
preventing the user from accessing computer resources of the consumer device; or
preventing the user from using functionalities of the consumer device.
10. A computer system comprising:
a data collector performing:
collecting a plurality of user-specific parameter values as a user attempts to access a consumer device; and
a computer processor performing:
determining at least two of the plurality of user-specific parameter values that uniquely identify the user;
determining two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong;
retrieving, from a reference database, at least two user-specific reference values that belong to the two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong and that have been stored in the reference database for the user;
determining whether the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values; and
in response to determining that the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values, granting, to the user, access to the consumer device.
11. The computer system of claim 10, wherein the computer processor further performs:
ranking the plurality of user-specific parameter values collected as the user attempts to access the consumer device based on a date when the plurality of user-specific parameter values was collected to determine whether the plurality of user-specific parameter values is to be used to determine whether to grant, to the user, access to the consumer device; and
ranking the at least two user-specific reference values based on a date when the at least two user-specific reference values were stored in the reference database to determine whether the at least two user-specific reference values are to be used to compare with the plurality of user-specific parameter values; and
wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values are performed automatically or manually by an authenticator.
12. The computer system of claim 11, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a noisy environment are based on assigning high ranks to values obtained as results of a contact with a sensor, and assigning low ranks to values collected as results of an object scanning.
13. The computer system of claim 11, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a low light environment are based on assigning high ranks to values collected using a voice and speech recognition device, and assigning low ranks to values collected as results of a contact with a sensor.
14. The computer system of claim 10,
wherein the consumer device is any one of: a computerized security device, a motorized vehicle, a motorized machinery, a domestic appliance, a bank kiosk, a television set, a setup box, a play station, a movie recorder, a computer device, a portable device, a smart phone, or a digital camera;
wherein granting, to the user, access to the consumer device comprises one or more of:
positively authenticating the user with a security system of the consumer device;
determining that the user is capable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
permitting to continue operating the consumer device;
permitting to withdraw funds from a bank account using the consumer device;
permitting to deposit funds to a bank account using the consumer device;
permitting to watch a specially-rated television program on the consumer device;
permitting to record a specially-rated television program on the consumer device;
permitting to play a specially-rated program on the consumer device;
permitting to access computer resources of the consumer device; or
permitting to use functionalities of the consumer device.
15. The computer system of claim 10,
wherein the plurality of user-specific parameter values includes two or more of: user credentials, a textual-based parameter value, a digital image, a biometric parameter value, a physiological parameter value, or a behavioral parameter value.
16. The computer system of claim 10, wherein the computer processor further performs:
in response to determining that the at least two of the plurality of user-specific parameter values do not match the at least two user-specific reference values, denying, the user, access to the consumer device;
wherein denying, the user, access to the consumer device comprises one or more of:
refusing to positively authenticate the user with a security system of the consumer device;
determining that the user is incapable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
turning off the consumer device;
preventing the user from withdrawing funds from a bank account using the consumer device;
preventing the user from depositing funds to a bank account using the consumer device;
preventing the user from watching a specially-rated television program on the consumer device;
preventing the user from recording a specially-rated television program on the consumer device;
preventing the user from playing a specially-rated program on the consumer device;
preventing the user from accessing computer resources of the consumer device; or
preventing the user from using functionalities of the consumer device.
17. One or more non-transitory computer storage media storing instructions which, when executed by one or more computer processors, cause performing:
collecting a plurality of user-specific parameter values as a user attempts to access a consumer device;
determining at least two of the plurality of user-specific parameter values that uniquely identify the user;
determining two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong;
retrieving, from a reference database, at least two user-specific reference values that belong to the two or more parameter categories to which the at least two of the plurality of user-specific parameter values belong and that have been stored in the reference database for the user;
determining whether the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values; and
in response to determining that the at least two of the plurality of user-specific parameter values match the at least two user-specific reference values, granting, to the user, access to the consumer device.
18. The one or more non-transitory computer storage media of claim 17, storing additional instructions which, when executed by one or more computer processors, cause performing:
ranking the plurality of user-specific parameter values collected as the user attempts to access the consumer device based on a date when the plurality of user-specific parameter values was collected to determine whether the plurality of user-specific parameter values is to be used to determine whether to grant, to the user, access to the consumer device; and
ranking the at least two user-specific reference values based on a date when the at least two user-specific reference values were stored in the reference database to determine whether the at least two user-specific reference values are to be used to compare with the plurality of user-specific parameter values; and
wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values are performed automatically or manually by an authenticator.
19. The one or more non-transitory computer storage media of claim 18, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a noisy environment are based on assigning high ranks to values obtained as results of a contact with a sensor, and assigning low ranks to values collected as results of an object scanning.
20. The one or more non-transitory computer storage media of claim 18, wherein the ranking of the plurality of user-specific parameter values and the ranking of the at least two user-specific reference values in a low light environment are based on assigning high ranks to values collected using a voice and speech recognition device, and assigning low ranks to values collected as results of a contact with a sensor.
21. The one or more non-transitory computer storage media of claim 17,
wherein the consumer device is any one of: a computerized security device, a motorized vehicle, a motorized machinery, a domestic appliance, a bank kiosk, a television set, a setup box, a play station, a movie recorder, a computer device, a portable device, a smart phone, or a digital camera;
wherein granting, to the user, access to the consumer device comprises one or more of:
positively authenticating the user with a security system of the consumer device;
determining that the user is capable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
permitting to continue operating the consumer device;
permitting to withdraw funds from a bank account using the consumer device;
permitting to deposit funds to a bank account using the consumer device;
permitting to watch a specially-rated television program on the consumer device;
permitting to record a specially-rated television program on the consumer device;
permitting to play a specially-rated program on the consumer device;
permitting to access computer resources of the consumer device; or
permitting to use functionalities of the consumer device.
22. The one or more non-transitory computer storage media of claim 17, storing additional instructions which, when executed by one or more computer processors, cause performing:
in response to determining that the at least two of the plurality of user-specific parameter values do not match the at least two user-specific reference values, denying, the user, access to the consumer device;
wherein denying, the user, access to the consumer device comprises one or more of:
refusing to positively authenticate the user with a security system of the consumer device;
determining that the user is incapable of performing one or more functions with respect to the consumer device, including starting the consumer device, operating the consumer device, or accessing resources of the consumer device;
turning off the consumer device;
preventing the user from withdrawing funds from a bank account using the consumer device;
preventing the user from depositing funds to a bank account using the consumer device;
preventing the user from watching a specially-rated television program on the consumer device;
preventing the user from recording a specially-rated television program on the consumer device;
preventing the user from playing a specially-rated program on the consumer device;
preventing the user from accessing computer resources of the consumer device; or
preventing the user from using functionalities of the consumer device.