All The Claims

1. The virus LAVMAL comprising RNA corresponding to the cDNA of FIGS. 7A-7I.
2. The cDNA of FIGS. 7A-7I.
3. A DNA recombinant comprising the cDNA of claim 2.
4. A probe containing a nucleic acid sequence hybridizable with RNA of said LAVMAL virus of claim 1.
5. A method for identifying the presence in a host tissue of LAV virus which comprises hybridizing RNA obtained from said tissue with said probe of claim 4.
6. The method of claim 5, wherein said probe can hybridize with RNA from said LAVMAL virus to identify said LAVMAL virus.
7. A peptide or fragment thereof whose amino acid sequence is encoded by an open reading frame of a cDNA sequence of the LAVMAL virus of claim 1.
8. The peptide of claim 7 encoded by a cDNA sequence from amino-acyl residue 37 to amino-acyl residue 130, or from amino-acyl residue 211 to amino-acyl residue 289, or from amino-acyl residue 488 to amino-acyl residue 530 of FIGS. 3A-3F and 7A-7I.
9. The peptide of claim 7 encoded by a cDNA sequence from amino-acyl residue 490 to amino-acyl residue 620 or from amino-acyl residue 680 to amino-acyl residue 700 of FIGS. 3A-3F and 7A-7I.
10. The peptide of claim 7 which comprises a protein or glycoprotein whose amino acid sequence is encoded by all or part of one of the following cDNA sequences of FIGS. 3A-3F and 7A-7I:
OMP or gp110 proteins, including precursors: 1 to 530;
OMP or gp110 without precursor: 34-530; and
TMP or gp41 protein: 531-877.
11. The peptide of claim 10 encoded by all or part of one of the following cDNA sequences of FIGS. 3A-3F and 7A-7I: 37-130, 211-289, 488-530, 490-620 or 680-700.
12. A method for the in vitro detection of the presence of an antibody directed against a LAV virus in a human body fluid, which comprises: contacting said body fluid with an antigen obtained from said virus LAVMAL of claim 1, said antigen consisting of a peptide or a fragment thereof whose amino acid sequence is encoded by an open reading frame of a cDNA sequence of FIGS. 7A-7I; and then detecting the immunological reaction between said antigen and said antibody.
13. The method of claim 12 wherein said antigen detects said LAVMAL virus of claim 1.
14. The method of claim 12 which comprises the steps of:
a) depositing a predetermined amount of said antigen into a cup of a titration microplate;
b) introducing increasing dilutions of said body fluid into said cup;
c) incubating said microplate;
d) washing the microplate with a buffer;
e) adding into said cup a labelled antibody directed against blood immunoglobulins; and then
f) determining whether an antigen-antibody-complex has formed in said cup which is indicative of the presence of a LAV antibody in said body fluid.
15. A diagnostic kit for the in vitro detection of antibodies against a LAV virus, which kit comprises: an antigen consisting of a peptide of claim 7.
16. The kit of claim 15 wherein the antigen consists of a peptide of said LAVMAL virus of claim 1, encoded by the open reading frame of a cDNA sequence of said LAVMAL virus.
17. An immunogenic composition comprising: an antigen of the LAVMAL virus of claim 1 or an immunogenic peptide or fragment thereof encoded by RNA of said virus; and a physiologically acceptable carrier.
18. The immunogenic composition of claim 17 wherein said peptide is the gp110 envelope glycoprotein or a fragment thereof.
19. The immunogenic composition of claim 17 wherein the peptide comprises a protein or glycoprotein hwose amino acid sequence is encoded by all or part of one of the following cDNA sequences of FIGS. 3a-3F and 7A-7I:
OMP or gp110 proteins, including precursors: 1 to 530;
OMP or gp110 without precursor: 34-530; and
TMP or gp41:531-877.
20. The composition of claim 19 wherein the protein or glycoprotein is encoded by all or part of one of the following cDNA sequences of FIGS. 3A-3F and 7A-7I: 37-130, 211-289, 488-530, 490-620 or 680-700.
21. An antibody formed against a peptide of claim 7.
22. A cell transformed with a DNA recombinant of claim 3.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A hydrogen source comprising one or more hydrogen generating elements arranged in a pressure vessel and an ignition control system associated with the hydrogen generating element(s), wherein the one or more hydrogen generating elements comprise a plurality of pellets of an ignitable chemical mixture that on thermal decomposition evolve hydrogen gas, wherein the ignition control system comprises a plurality of igniters arranged to ignite the pellets on an individual basis, and wherein the ignition control system is arranged to control the rate of ignition of the pellets.
2. A hydrogen source according to claim 1, wherein the hydrogen source is man portable.
3. (canceled)
4. A hydrogen source according to claim 1, wherein thermal insulation is provided within the pressure vessel to reduce heat transfer from the one or more hydrogen generating elements.
5. A hydrogen source according to claim 1, further comprising a regulator to control the flow of evolved hydrogen out from the pressure vessel.
6. A hydrogen source according to claim 5, wherein the regulator is integrated within the pressure vessel.
7. A hydrogen source according to claim 1, further comprising a filter to purify the evolved hydrogen.
8. A hydrogen source according to claim 7, wherein the filter is integrated within the pressure vessel.
9. A hydrogen source according to claim 7, wherein the filter is one of an activated carbon filter, a porous stainless steel filter or a sintered metal filter.
10. A hydrogen source according to claim 1, wherein the or each hydrogen generating element comprises a pellet holder provided with one or more recesses.
11. A hydrogen source according to claim 10, wherein the pellet holder comprises a rigid, porous material.
12. A hydrogen source according to claim 1, wherein at least one hydrogen generating element further comprises a gas handling layer provided with channels arranged to direct the flow of evolved hydrogen.
13. A hydrogen source according to claim 12, wherein the gas handling layer comprises a metallic layer arranged to contact the inner surface of the pressure vessel, thereby conducting heat to the exterior.
14. A hydrogen source according to claim 4, wherein the thermal insulation comprises one or more of a felt layer, a ceramic, asbestos or rockwool.
15. A hydrogen source according to claim 1, wherein the one or more igniters comprise heated resistance wires or pyrotechnic igniters.
16. A hydrogen source according to claim 1, wherein the igniters are placed in contact with the pellets.
17. A hydrogen source according to claim 1, wherein the ignition control system comprises activation means to activate the igniters, which means are selected from a battery, an electrochemical cell, a fuel cell, capacitor or a power supply.
18. A hydrogen source according to claim 1, wherein the ignition control system further comprises a pressure transducer to determine the pressure of hydrogen within the pressure vessel.
19. A hydrogen source according to claim 18, wherein the output from the pressure transducer is used to trigger the decomposition of the pellets in order to maintain the pressure of hydrogen at a pre-set level or in response to demand from external equipment.
20. A hydrogen source according to claim 18, wherein the activation of the igniters is prevented if the output from the pressure transducer indicates that the pressure within the pressure vessel is above a safe limit.
21. A hydrogen source according to claim 1, wherein the ignition control system further comprises a temperature probe to determine the temperature within the pressure vessel.
22. A hydrogen source according to claim 21, wherein the activation of the igniters is prevented if the output from the temperature probe indicates that the temperature within the pressure vessel is above a safe limit.
23. A hydrogen source according to claim 1, wherein the pressure vessel is adapted to be rechargeable with replacement pellets for re-use.
24-25. (canceled)
26. Portable equipment comprising a hydrogen source according to claim 1.
27. Equipment according to claim 26 in the form of a fuel cell system.
28. (canceled)
29. A hydrogen source comprising one or more hydrogen generating elements arranged in a pressure vessel and an ignition control system associated with the hydrogen generating element(s), wherein the one or more hydrogen generating elements comprise a plurality of pellets of an ignitable chemical mixture that on thermal decomposition evolve hydrogen gas, wherein the ignition control system is arranged to control the rate of ignition of the pellets, and wherein the ignition control system comprises a plurality of igniters, one or more igniters being associated with each individual pellet to permit successive or simultaneous ignition of pellets in a controllable manner.
30. A hydrogen source comprising one or more hydrogen generating elements arranged in a pressure vessel and an ignition control system associated with the hydrogen generating element(s), wherein the one or more hydrogen generating elements comprise a plurality of pellets of an ignitable chemical mixture that on thermal decomposition evolve hydrogen gas, wherein the ignition control system comprises a plurality of igniters arranged to ignite the pellets on an individual basis and the pellets are sufficiently spaced or separated from one another to prevent cross-ignition, and wherein the ignition control system is arranged to control the rate of ignition of the pellets.

I claim:

1. A method of inducing a specific protective immune response to the Human Immunodeficiency Virus in a human being, comprising the steps of:
isolating antigen-presenting dendritic cells from said human being, and pulsing said dendritic cells with synthetic peptides that bind said human being’s Class I Major Histocompatiblity types, said peptides corresponding to conserved structural and functional gene products of said Human Immunodeficiency Virus, and injecting said pulsed dendritic cells intravenously into said human being, and injecting said human being with substantially pure Chikungunya Virus.
2. The method of claim 1 wherein said substantially pure Chikungunya Virus is purified by passing said virus through living cells with an active transmembrane gradient; and passing said virus through subhuman primates to confirm the elimination of neurovirulence from the virus.
3. The method of claim 2 wherein said cells with an active transmembrane gradient are African Green Monkey Kidney Cells are infected with virus at less than 5 plaque-forming unitsml.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An electrical fuse circuit comprising:
a plurality of groups, each of the groups including:
a capacitor composing an electrical fuse;
a write circuit breaking an insulating film of said capacitor by applying a voltage across both terminals of said capacitor in accordance with one of a plurality of write signals; and
a precharge circuit precharging with respect to the terminal of said capacitor,
wherein the precharge circuit is coupled to a low-voltage-side terminal of the capacitor,
wherein each of the plurality of write signals is supplied to the corresponding write circuit of each of the plurality of groups at different timings with one another and during a period of time when a precharge operation is not being performed, and
wherein the precharge operation is performed during a period of time when the write signals are inactive.
2. The electrical fuse circuit according to claim 1
wherein said write circuits in the respective groups apply voltage to said capacitors in accordance with the write signal, respectively, at mutually different timings.
3. The electrical fuse circuit according to claim 1,
wherein, otherwise, said precharge circuit applies a precharge potential to a second terminal of said capacitor in accordance with a precharge signal to put the second terminal of said capacitor into a floating state.
4. A memory device comprising:
an electrical fuse circuit described in claim 1;
a normal memory cell array including a plurality of memory cells; and
a redundant memory cell array including a memory cell to replace the memory cell in said normal cell array;
wherein a capacitor in said electrical fuse circuit memorizes an address of the memory cell in said normal memory cell array to be replaced.
5. The electrical fuse circuit according to claim 2,
wherein the precharge operation is performed to the terminal of said capacitor during a period of time when said write circuits in the respective groups apply voltage to said capacitors in accordance with the write signal, respectively, at mutually different timings.
6. The electrical fuse circuit according to claim 3,
wherein said precharge circuit includes a first field-effect transistor connected to the terminal of said capacitor with a drain thereof and connected to the precharge potential with a source thereof.
7. The electrical fuse circuit according to claim 3,
wherein said write circuit breaks the insulating film of said capacitor by applies a first potential to a first terminal of said capacitor and applying a second potential having a lower potential than that of the first potential to a second terminal of said capacitor, in accordance with the write signal.
8. The electrical fuse circuit according to claim 3, further comprising a second field-effect transistor connected to between the drain of the first field-effect transistor and the second terminal of said capacitor.
9. The electrical fuse circuit according to claim 6, further comprising a second field-effect transistor connected to between the drain of the first field-effect transistor and the terminal of said capacitor.
10. The electrical fuse circuit according to claim 7,
wherein, otherwise, said write circuit applies the second potential to the second terminal of said capacitor in accordance with the write sign alto put the second terminal of said capacitor into a floating state.
11. The electrical fuse circuit according to claim 10,
wherein said write circuit includes a first field-effect transistor connected to the write signal with a gate thereof and connected to a second terminal of said capacitor with a source thereof.
12. The electrical fuse circuit according to claim 8,
wherein said precharge circuit includes a third field-effect transistor connected to the second terminal of said capacitor via said second field-effect transistor with a drain thereof and to a precharge potential with a source thereof.
13. An electronic part comprising a memory device described in claim 4, said memory device including an electrical fuse circuit, a normal memory cell array and a redundant memory cell array and a semiconductor memory chip provided with the electrical fuse circuit, the normal memory cell array and the redundant memory cell array therein,
wherein the semiconductor memory chip is mounted in a package.
14. The electronic part according to claim 13,
wherein a chip having a memory controller controlling an operation of a write circuit with respect to the semiconductor memory chip is mounted in the same package as of said semiconductor memory device.