1461171877-fdd9d48b-311d-4564-92f6-c8608d730d15

1. A video coding method applied to an original video sequence in which the successive frames or video object planes (VOPs) include one or several arbitrarily shaped video objects (VOs) defined in each VOP by their texture and motion components and an additional shape component, said method comprising the following steps
(a) a non object-oriented coding step, applied to a small number of frames of the video sequence ;
(b) an object-oriented coding step, applied to all the frames of the sequence that follow said small number of frames;
(c) a sequencing step, provided for controlling that said non object-oriented and object-oriented coding steps are respectively applied to the appropriate frames, in order to generate a coded bitstream including non object-oriented coded data corresponding to said small number of frames followed by object-oriented coded data corresponding to said following frames.
2. A coding method according to claim 1, in which said number of frames is equal to two.
3. A coding method according to claim 1, wherein said coded bitstream also includes an information about the number of regions of interest in the original video sequence.
4. A coding method according to claim 3, wherein said information about the number of regions of interest is given at the picture level.
5. A video decoding method applied to a coded bitstream corresponding to an original video sequence in which the successive frames or video object planes (VOPs) include one or several arbitrarily shaped video objects (VOs) defined in each VOP by their texture and motion components and an additional shape component and have been coded by means of a video coding method comprising the following steps:
(a) a non object-oriented coding step, applied to a small number of frames of the video sequence ;
(b) an object-oriented coding step, applied to all the frames of the sequence that follow said small number of frames;
(c) a sequencing step, provided for controlling that said non object-oriented and object-oriented coding steps are respectively applied to the appropriate frames, in order to generate a coded bitstream including non object-oriented coded data corresponding to said small number of frames followed by object-oriented coded data corresponding to said following frames;
said decoding method itself comprising the following steps
(1) a first decoding step, applied to said non object-oriented coded data of the coded bitstream that correspond to said small number of frames of the original video sequence;
(2) a spatio-temporal segmentation step applied to said non object-oriented coded data of the coded bitstream that correspond to said small number of frames and provided for reconstructing the missing shape component of the VOs;
(3) a second decoding step, applied to said object-oriented coded data of the coded bitstream that correspond to said following frames;
(4) a sequencing step, provided for controlling that said decoding and segmentation steps are respectively applied to the appropriate frames.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. Primers for amplifying an HLA gene.
2. The primers of claim 1, wherein the HLA gene is HLA A, B or DRB1 or an exon therein.
3. The primers of claim 2, wherein the HLA-A primers have sequences shown in SEQ ID NOS: 14-15, the HLA-A exon 2 primers have the sequences shown in SEQ ID NOS: 20-21 and the HLA-A exon 3 primers have the sequences shown in SEQ ID NOS: 22-26.
4. The primers of claim 2, wherein the HLA-B primers have sequences shown in SEQ ID NOS: 16-19, the HLA-B exon 2 primers have the sequences shown in SEQ ID NOS: 27-28 and the HLA-B exon 3 primers have the sequences shown in SEQ ID NOS: 29-31.
5. The primers of claim 2, wherein the HLA-DRB1 primers have sequences shown in SEQ ID NOS: 32-37 and the HLA-B exon 2 primers have the sequences shown in SEQ ID NOS: 38-47.
6. Hybridization probes for detecting single nucleotide polymorphisms (SNPs) in an HLA gene, comprising:
about a 9-15mer oligonucleotide complementary to a region containing the SNP; and
5\u2032 and 3\u2032 flanking sequences.
7. The hybridization probes of claim 6, wherein the flanking sequences are oligo-thymidines or an oligo-thymidine-like polyanionic polymer.
8. The hybridization probes of claim 6, wherein the SNPs are located in HLA-A exon 2 or exon 3, HLA-B exon 2 or exon 3 or HLA-DRB1 exon 2.
9. The hybridization probes of claim 8, wherein the HLA-A exon 2 probes has the sequence shown in SEQ ID NO: 49 and the HLA-A exon 3 probes have the sequences shown in SEQ ID NOS: 101-153.
10. The hybridization probes of claim 8, wherein the HLA-B exon 2 probes have the sequences shown in SEQ ID NOS: 154-237 and the HLA-B exon 3 probes have the sequences shown in SEQ ID NOS: 238-239.
11. A microarray device for allelotyping an HLA gene, comprising:
a substrate having a cationic surface; and
a monolayer comprising one or more of the hybridization probes of claim 6 adsorbed thereto.
12. The microarray device of claim 11, wherein the cationic surface comprises an aminosilane, a quanidinium, tin oxide, aluminum oxide or zirconium oxide or other equivalently charged moiety.
13. The microarray device of claim 11, wherein the substrate is glass, plastic or metal.
14. The microarray device of claim 11, further comprising:
an oligo-thymidine co-absorbed with the hybridization probes.
15. The microarray device of claim 14, wherein the oligo-thymidine has about 20 to about 40 thymidine.
16. The microarray device of claim 14, further comprising:
a fluorescent dye linked to the oligo-thymidine
17. The microarray device of claim 11, further comprising:
a capping agent.
18. A kit for population-scale HLA genotyping, comprising:
gene-specific primers for amplifying an HLA gene; and
the microarray device of claim 11.
19. The kit of claim 18, wherein the primers have the sequences shown in SEQ ID NOS: 14-47.
20. The kit of claim 18, further comprising:
buffers and polymerases for a PCR reaction or a fluorescent dye or a combination thereof.
21. A system for real-time high throughput population-scale HLA allelotyping in a field environment, comprising:
the microarray device of claim 11;
means for collecting and purifying DNA samples from individuals comprising a population;
means for generating by PCR cRNA target amplicons of one or more HLA genes of interest from the collected DNA; and
means for assigning an HLA allelotype to each individual HLA gene of interest;
wherein individual means and devices comprising said system are portable and operable in real time within the field environment.
22. The system of claim 21, wherein the HLA gene is HLA-A, HLA-B or HLA-DRB1.
23. The system of claim 21, wherein the means for collecting DNA samples comprises:
a container suitable to receive a buccal wash sample, a buccal swab sample or a blood sample collected from the individuals.
24. The system of claim 21, wherein the means for generating target amplicons comprises HLA gene-specific primers for amplifying the HLA gene of interest.
25. The system of claim 24, wherein the gene-specific primers have sequences shown in SEQ ID NOS: 14-47.
26. The system of claim 21, wherein said means for assigning an HLA-allelotype to each individual comprises:
an imaging device adapted to detect hybridization patterns formed on the microarray device after hybridization of the target to the hybridization probes adsorbed thereto; and
pattern recognition software comprising a set of algorithms adapted to recognize the imaged hybridization patterns as HLA allelotypes.
27. The system of claim 26, wherein the hybridization probes have sequences shown in SEQ ID NOS: 48-289.
28. The system of claim 21, wherein real time high throughput genotyping is about 200 to about 300 HLA allelotypes per hour per system operated.
29. A method for real time population-scale HLA allelotyping in a field environment, comprising:
collecting DNA from one or members of the population;
purifying the DNA for analysis;
generating a target amplicon from an HLA gene of interest comprising the DNA using gene specific primers;
contacting the hybridization probes comprising the microarray of claim 11 with the target; and
imaging the hybridization pattern formed after the contact wherein each HLA allelotype has a pattern associated therewith.
30. The method of claim 29, further comprising storing the collected DNA.
31. The method of claim 29, wherein the DNA is collected from blood, with a buccal wash or with a buccal swab.
32. The method of claim 29, wherein the gene-specific primers have sequences shown in SEQ ID NOS: 14-47 and the hybridization probes have sequences shown in SEQ ID NOS: 48-289.
33. The method of claim 29, further comprising one or both of:
assessing a risk of infection by a biological agent or weapon for each individual based on the assigned allelotype; or
assessing a response to a particular vaccine against the biological agent or weapon by each individual; or
34. The method of claim 29, wherein the allelotype assigned to each individual of the population comprises a means of identification thereof.

1461171867-a810f10e-d0cb-4753-b4f5-e476a2aeec0d

1-20. (canceled)
21. A telecommunications system comprises:
a processor configured to receive a plurality of data packets from a remote telecommunications system via a computer network, wherein the processor is also configured to determine a telephone number in response to a first set data packets from the plurality of data packets, wherein the processor is also configured to determine call-back data associated with the remote telecommunications system in response to a second set of data packets from the plurality of data packets, and wherein the processor is also configured to determine message data in response to a third set of data packets from the plurality of data packets; and
a memory coupled to the processor, wherein the memory is configured to store the message data and the call-back data, wherein the message data and the call-back are associated with telephone number;
wherein the processor is also configured to send a plurality of data packets to the remote telecommunications system via the computer network in response to the call-back data.
22. The telecommunications system of claim 21 wherein the message data is selected from the group consisting of: voice data, facsimile data, file data.
23. The telecommunications system of claim 21 wherein data stored in the first set of data packets is selected from the group consisting of: an extension number, a user name, a receiver name, a computer address, receiver’s initials, a business group name.
24. The telecommunications system of claim 23 wherein the memory is also configured to store an association between the data stored in the first set of data packets and the telephone number.
25. The telecommunications system of claim 21 wherein data stored in the second set of data packets is selected from the group consisting of: an IP header, caller identifiable data, a telephone number, a user name, a caller name, an extension number, a computer address.
26. The telecommunications system of claim 25 wherein the memory is also configured to store an association between the data stored in the second set of data packets and the call-back data.
27. The telecommunications system of claim 21 wherein data stored in the plurality of data packets sent to the remote telecommunications system is selected from the group consisting: telephone number, voice data, caller identifying data, voice prompt data, voice mail data.
28. A method for a telecommunications apparatus comprises:
receiving a plurality of data packets from a remote telecommunications system via a computer network,
determining a receiving party identifier in response to a first set of data packets from the plurality of data packets;
dialing a telephone number associated with the receiving party identifier in response to the receiving party identifier;
determining a calling party identifier in response to at least one data packet from the plurality of data packets;
determining message data in response to a second set of data packets;
storing the message data and the calling party identifier in a messaging system; and
sending a responsive plurality of data packets to the remote telecommunications system via a computer network in response to the calling party identifier.
29. The telecommunications apparatus of claim 28 wherein the message data is selected from the group consisting of: analog data, digital data.
30. The telecommunications apparatus of claim 28 wherein the receiving party identifier is selected from the group consisting of: an extension number, a user name, a receiver name, a computer address, receiver’s initials, a business group name.
31. The telecommunications apparatus of claim 30 further comprising determining the telephone number associated with the receiving party in response to an association between the receiving party identifier and the telephone number stored in a memory.
32. The telecommunications apparatus of claim 28 wherein the calling party identifier is selected from the group consisting of: an IP header, caller identifiable data, a telephone number, a user name, a caller name, an extension number, a computer address.
33. The telecommunications apparatus of claim 32 further comprising:
determining a computer network address associated with the remote telecommunications system in response to the calling party identifier,
wherein sending the responsive plurality of data packets to the remote telecommunications system via a computer network comprises sending the responsive plurality of data packets to the remote telecommunications system using the computer network address.
34. The telecommunications apparatus of claim 28 wherein the responsive plurality of data packets include message data selected from the group consisting: a telephone number, voice data, receiving party identifying data, voice prompt data, voice mail data.
35. A computer program product for a telecommunications system including a processor comprises:
code that directs the telecommunications system to receive a plurality of data packets from a remote telecommunications system via a computer network;
code that directs the telecommunications system to determine a receiving party identifier in response to a first set of data packets from the plurality of data packets;
code that directs the telecommunications system to dial a telephone number associated with the receiving party identifier in response to the receiving party identifier;
code that directs the telecommunications system to determine a calling party identifier in response to at least one data packet from the plurality of data packets;
code that directs the telecommunications system to determine message data in response to a second set of data packets; and
code that directs the telecommunications system to store the message data and the calling party identifier;
wherein the codes reside on a tangible media.
36. The computer program product of claim 35 further comprising:
code that directs the telecommunications system to send a responsive plurality of data packets to the remote telecommunications system via a computer network in response to the calling party identifier.
37. The computer program product of claim 35 wherein the message data is selected from the group consisting of: digitized analog data, digital data.
38. The computer program product of claim 35 wherein the receiving party identifier is selected from the group consisting of: an extension number, a user name, a receiver name, a business group name.
39. The computer program product of claim 38 further comprising code that directs the telecommunications system to determine the telephone number associated with the receiving party in response to a stored association between the receiving party identifier and the telephone number.
40. The computer program product of claim 35 wherein the calling party identifier is selected from the group consisting of: an IP header, a packet header, caller identifiable data, a telephone number, a user name, a caller name, an extension number.
41. The computer program product of claim 35 further comprising:
code that directs the telecommunications system to determine a computer network address associated with the remote telecommunications system in response to the calling party identifier, and
code that directs the telecommunications system to send the responsive plurality of data packets to the remote telecommunications system using the computer network address.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A positive planographic printing plate precursor, comprising a support having disposed thereon a positive recording layer containing (A) a water-insoluble and alkali-soluble resin, (B) an infrared absorbent and (C) an organic quaternary ammonium salt, wherein solubility of the recording layer in an aqueous alkali solution is increased by exposure to an infrared laser.
2. The positive planographic printing plate precursor according to claim 1, wherein the (C) organic quaternary ammonium salt has in a molecule thereof at least one of an aryl group and a carbonyl group.
3. The positive planographic printing plate precursor according to claim 1, wherein the (C) organic quaternary ammonium salt has in a molecule thereof both an aryl group and a carbonyl group.
4. The positive planographic printing plate precursor according to claim 1, wherein the (C) organic quaternary ammonium salt is represented by the following general formula (I):
wherein R1, R2, R3 and R4 are each independently an organic group having one or more carbon atoms, and they may be bonded with each other to form a ring.
5. The positive planographic printing plate precursor according to claim 4, wherein at least one of R1, R2, R3 and R4 is selected from the group consisting of the following structures:
wherein Ar1 represents an aryl group, R5, R6 and R7 represent independently a hydrogen atom or an organic group having one or more carbon atoms, at least two of which are selected from an organic group which is not a hydrogen atom, and R5, R6 and R7 may be bonded with each other to form a ring.
6. The positive planographic printing plate precursor according to claim 4, wherein at least one of R1, R2, R3 and R4 is selected from the group consisting of the following structures:
wherein R8, R9 and R10 represent independently a hydrogen atom or an organic group having one or more carbon atoms, at least two of which are selected from an organic group which is not a hydrogen atom, and R8, R9 and R10 may be bonded with each other to form a ring.
7. The positive planographic printing plate precursor according to claim 6, wherein R8 is an aryl group.
8. The positive planographic printing plate precursor according to claim 4, wherein at least one of R1, R2, R3 and R4 is selected from the group consisting of the following structures:
wherein Ar2 represents an aryl group, R11 and R12 represent independently a hydrogen atom or an organic group having one or more carbon atoms, Ar2, R11 and R12 may be bonded with each other to form a ring, and R13, R14 and R15 represent independently a hydrogen atom or an organic group having one or more carbon atoms, at least one of R13, R14 and R15 is a non-aromatic cyclic substituent, or any adjacent two of R13, R14 arid R15 may be bonded with each other to form a ring.
9. The positive planographic printing plate precursor according to claim 4, wherein the (C) organic quaternary ammonium salt represented by the general formula (I) is at least one selected from the group consisting of the following ammonium salts:
10. The positive planographic printing plate precursor according to claim 1, wherein the (C) organic quaternary ammonium salt is contained at 0.1 to 40% by weight of the total solid component of the positive recording layer.
11. A positive planographic printing plate precursor, comprising a support having disposed thereon at least two positive recording layers containing a water-insoluble and an alkali-soluble resin and an infrared-absorbing dye, with solubility of the recording layer in an aqueous alkali solution being increased by exposure to an infrared laser, wherein a coating amount of an upper positive recording layer is in the range of 0.05 to 0.45 gm2 and wherein a water-insoluble and alkali-soluble resin used in a lower recording layer and a water-insoluble and alkali-soluble resin used in the upper recording layer have different solubilities in a coating solvent.
12. The positive planographic printing plate precursor according to claim 11, wherein the positive recording layer having a coating amount of 0.05 to 0.45 gm2 is located at a position nearest to a surface among a plurality of positive recording layers.
13. The positive planographic printing plate precursor according to claim 11, wherein an infrared-absorbing dye contained in an upper positive recording layer has high infrared transmittance.
14. The positive planographic printing plate precursor according to claim 13, wherein the dye having the high infrared transmittance is at least one selected from the group consisting of an indoaniline dye, a cyanine dye, a merocyanine, an oxonol dye, a porphyrin derivative, an anthraquinone dye, a merostyryl dye, a pyrylium compound, a diphenyl and triphenyl azo compound and a squarylium derivative.
15. The positive planographic printing plate precursor according to claim 11, wherein the water-insoluble and alkali-soluble polymer compound is selected from the group consisting of a homopolymer containing an acidic group on a main chain andor a side chain, a copolymer thereof and a mixture thereof, and the acidic group which is at least one of a phenol group and a sulfonamide group.
16. The positive planographic printing plate precursor according to claim 11, wherein two or more kinds of water-insoluble and alkali-soluble resins are used in combination as the water-insoluble and an alkali-soluble resin.
17. The positive planographic printing plate precursor according to claim 11, wherein the recording layer further contains at least one selected from the group consisting of an onium salt, an o-quinonediazide compound, an aromatic sulfone compound, an aromatic sulfonic ester compound, a nonionic surfactant, an amphoteric surfactant, a printing out agent, an plasticizer and a dye and a pigment as an image coloring agent.
18. The positive planographic printing plate precursor according to claim 11, wherein each of an upper recording layer and a lower recording layer is prepared by a process selected from: a process comprising the steps of coating the lower recording layer coating solution on the support and coating an upper recording layer coating solution thereon; and a process of overlap-coating two recording layers.
19. The positive planographic printing plate precursor according to claim 11, wherein the support is one of a polyester film and an aluminum plate.