All The Claims

What is claimed is:

1. An apparatus for use in a cooling system comprising:
first means for transporting thermal energy from a source and
second means for communicating electro-optical radiation from said source.
2. The invention of claim 1 wherein said apparatus includes a manifold.
3. The invention of claim 2 wherein said first means includes means for directing a cooling fluid over a surface of said source.
4. The invention of claim 3 wherein said means for directing a cooling fluid over the surface of said source includes a plurality of conduits in said manifold.
5. The invention of claim 4 wherein said means for directing a cooling fluid over the surface of said source further includes an inlet in said manifold and an exhaust port in said manifold.
6. The invention of claim 2 wherein said second means includes an optically transparent aperture disposed in said manifold.
7. The invention of claim 6 wherein said second means includes material that is transparent to the waste electromagnetic energy generated by said source.
8. The invention of claim 7 wherein said waste electromagnetic energy is waste fluorescent radiation energy at a wavelength consistent with the operation of a laser system with which said apparatus is used.
9. The invention of claim 8 wherein said source is a slab of a solid-state laser.
10. A cooling system adapted for use with a laser, said cooling system comprising:
means for providing a cooling fluid and
a manifold at least partially transparent to fluorescent energy radiating from said laser and mounted in close physical proximity to the laser medium thereof, said manifold including an inlet, a plurality of nozzles, in communication with said inlet, for spraying said fluid on said laser medium and an exhaust port for retrieving said fluid after said fluid is sprayed on said laser medium.
11. The system of claim 10 further including means for deposition of significant portions of said fluorescent energy in either the cooling fluid or said manifold
12. The system of claim 11 including means for optically directing said fluorescent energy to an external environment without intermediate cooling means.
13. A method for cooling the laser medium of a laser system including the steps of:
providing a cooling fluid;
spraying said cooling fluid on said cavity to remove thermal energy therefrom; and
optically removing fluorescent energy from said laser medium without depositing said fluorescent energy in said cooling fluid.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. Method for producing hemin proteins comprising the following steps:
i) introducing, into plant cells, one or more nucleic acid molecule(s) each of which comprises at least one sequence encoding a protein component of a hemin protein of animal origin capable of reversibly binding oxygen or a variant or a portion of this protein component, and optionally a sequence encoding a selection agent;
ii) selecting the cells containing the nucleic acid encoding the protein component of the hemin protein;
iii) optionally propagating the transformed cells, either in culture or by regenerating whole transgenic or chimeric plants;
iv) recovering, and optionally purifying, a hemin protein comprising a complex of the protein or proteins encoded by the abovementioned nucleic acid with at least one iron-containing porphyrin nucleus, or a plurality of these complexes.
2. Method according to claim 1, characterized in that the hemin protein is a single polypeptide chain, for example myoglobin.
3. Method according to claim 1, characterized in that the hemin protein is a heterooligomer, the nucleic acid(s) comprising the sequences encoding each of the various protein units.
4. Method according to claim 3, characterized in that the hemin protein is human hemoglobin, or a derivative thereof, the nucleic acid(s) comprising sequences encoding and globin, or variants of or globin, the variants differing from the natural sequence in one or more amino acid substitution(s), deletion(s) or insertion(s).
5. Method according to claim 4, characterized in that the sequences encoding the various protein units, and globin, are contained within the same nucleic acid molecule.
6. Method according to claim 4, characterized in that the sequences encoding the various protein units, and globin, are contained within separate nucleic acid molecules.
7. Method according to any one of claims 1 to 6, characterized in that the introduction of the nucleic acid molecule(s) is carried out by transformation of the nuclear genome of the plant cell.
8. Method according to claim 7, characterized in that the sequence encoding the protein component comprises one or more sequence(s) encoding chloroplast targeting signals, or mitochondrial targeting signals.
9. Method according to claim 7, characterized in that the sequence encoding the protein component comprises one or more sequence(s) encoding an N-terminal signal peptide and optionally a signal responsible for retaining the protein in the endoplasmic reticulum, or a vacuolar targeting signal.
10. Method according to any one of claims 1 to 6, characterized in that the introduction of the nucleic acid is performed by a transformation of the mitochondrial or chloroplast genome.
11. Method according to any one of claims 1 to 9, characterized in that the nucleic acid comprises, in addition to the coding sequence(s), sequences for regulation of transcription which are recognized by plant cells.
12. Method according to any one of claims 4 to 11, characterized in that the coding sequence(s) encode(s) a hybrid molecule composed of at least the active parts of -globin and -globin.
13. Method according to any one of claims 1 to 12, characterized in that it comprises, between the propagation step and the recovery step, a step for detecting hemin proteins and in particular hemin proteins whose porphyrin nucleus consists of iron-containing protoporphyrin IX.
14. Method according to claim 13, characterized in that during the step for propagating plant cells, iron-containing protoporphyrin IX is added to the culture medium used for growing the cells.
15. Hemin protein having the capacity to reversibly bind oxygen, characterized in that it comprises at least one iron-containing porphyrin nucleus, of plant origin, and a protein component comprising at least one polypeptide chain, of animal origin.
16. Protein according to claim 15, characterized in that the iron-containing porphyrin nucleus is iron-containing protoporphyrin IX, or a protoporphyrin differing from protoporphyrin IX in the nature of the side chains carried by the p atoms of the pyrrole rings.
17. Protein according to claim 16, characterized in that the protein component comprises at least one – andor -globin polypeptide chain, or variants thereof comprising one or more amino acid substitution(s), deletion(s) or insertion(s), the hemin protein being capable of binding oxygen reversibly.
18. Protein according to claim 17, characterized in that the – or -globin chain, or variants thereof, comprises in addition a chloroplast targeting signal, a mitochondrial targeting signal, or a N-terminal signal peptide optionally in combination with a signal responsible for retaining protein in the endoplasmic reticulum, or a vacuolar targeting signal.
19. Protein according to claim 17, characterized in that each polypeptide chain lacks NH2-terminal methionine.
20. Protein according to any one of claims 17 to 19, characterized in that the protein component comprises at least four polypeptide chains of – andor -globin or variants thereof, each polypeptide chain being bound to an iron-containing protoporphyrin nucleus.
21. Protein according to claim 20, characterized in that it comprises 2 -globin chains and 2 -globin chains, or variants thereof.
22. Protein according to any one of claims 15 to 21, characterized in that it binds oxygen with an affinity of between 7 and 40 mm Hg, preferably 15 to 20 mm Hg.
23. Nucleic acid comprising:
i) one or more sequence(s) encoding a protein component of an animal hemin protein, the said protein having the capacity to reversibly bind oxygen, and
ii) sequences for regulation of transcription which are recognized by a plant cell, comprising a promoter and sequences for regulation of termination, and
iii) one or more sequence(s) encoding a targeting signal of plant origin.
24. Nucleic acid according to claim 23, characterized in that the regulatory sequences comprise one or more promoter(s) of plant origin.
25. Nucleic acid according to claim 23 or 25, characterized in that the sequences encoding the targeting signal encode a mitochondrial or chloroplast targeting peptide called transit peptide.
26. Nucleic acid according to claim 23 or 24, characterized in that the sequences encoding the targeting signal encode an N-terminal signal peptide of plant origin, optionally in combination with a sequence encoding an endoplasmic retention signal or a vacuolar targeting signal.
27. Nucleic acid according to any one of claims 23 to 26, characterized in that the coding sequence encodes human – or -globin, or a variant thereof differing from the natural sequence in one or more amino acid substitution(s), deletion(s) or replacement(s), or a portion of human – andor -globin.
28. Nucleic acid according to any one of claims 23 to 27, comprising, in addition, one or more intron(s), preferably of plant origin.
29. Nucleic acid according to any one of claims 23 to 28, characterized in that the sequence encoding the protein component is a cDNA.
30. Vector comprising one or more nucleic acid molecule(s) according to any one of claims 23 to 29.
31. Plant cells transformed in a stable manner by the nucleic acid according to any one of claims 23 to 29.
32. Plant cells capable of producing one or more hemin protein(s) according to any one of claims 15 to 22.
33. Plant cells according to claim 32, characterized in that they comprise nucleic acid comprising one or more sequence(s) encoding a protein component of the said hemin protein in association with one or more sequence(s) for regulation of transcription recognized by the cell.
34. Plant cells according to any one of claims 31 to 33, characterized in that they are a culture of plant cells, for example in liquid medium or immobilized cells, or a root culture.
35. Plant cells according to any one of claims 31 to 33, characterized in that they are cells which form part of a whole transformed plant.
36. Chimeric or transgenic plant capable of producing one or more hemin protein(s), for example hemoglobin or a derivative thereof, characterized in that it comprises cells according to any one of claims 31 to 33.
37. Seeds of transgenic plant according to claim 36.
38. Pharmaceutical product comprising one or more hemin protein(s) according to any one of claims 15 to 22 in association with a physiologically acceptable excipient.
39. Hemin proteins according to any one of claims 15 to 22 for use as medicament.
40. Hemin protein according to claim 39, for use in the treatment of conditions requiring an improvement in the transport of oxygen in the blood.
41. Use of a hemin protein according to any one of claims 15 to 22 for the preparation of a medicament for the treatment of conditions requiring an improvement in the transport of oxygen in the blood.
42. Use of a hemin protein according to any one of claims 15 to 22 in an industrial, cosmetic product or as chemical reagent.

1. A method of surveying, the method comprising the acts of:
detecting a barcode on a surveying mark associated with a position of interest, wherein the barcode is detected based on imaging by a surveying tool;
decoding the barcode to extract encoded data associated with feature information for the position of interest, the data including position data for the position of interest;
performing a surveying measurement to determine position data for the position of interest, the surveying measurement performed by a position detection module of the surveying tool; and
presenting the data associated with the position of interest to an operator of the surveying tool, wherein presenting includes displaying position data decoded from the barcode and displaying position data determined from the surveying measurement.
2. The method of claim 1, wherein the barcode is one of a two-dimensional barcode and a matrix code.
3. The method of claim 1, wherein the data extracted from the barcode relates to one of an elevation and a grade.
4. The method of claim 1, wherein the feature information for the position of interest includes at least one of a date of last entry, a longitude, a latitude, a location of a utility service, a location of a next position of interest, and user-provided data.
5. The method of claim 1, wherein presenting decoded data includes displaying the decoded data on the surveying tool, the decoded data associated with a barcode target identified by a target window displayed by the surveying tool.
6. The method of claim 1, further comprising labeling the position of interest with the barcode bearing the encoded data.
7. The method of claim 1, further comprising performing a surveying measurement for the position of interest based on orientation data decoded from the barcode.
8. The method of claim 7, further comprising evaluating a change in the position of interest based, at least in part, on the decoded data and surveying measurement, wherein evaluating includes comparing a result of the surveying measurement to data extracted from the barcode.
9. The method of claim 1, further comprising updating data provided by the barcode, wherein updating comprises:
encoding data for the position of interest into an updated barcode; and
labeling the position of interest with the updated barcode.
10. The method of claim 9, wherein the surveying tool further comprises a barcode printer and the updated barcode is printed from the barcode printer.
11. A surveying tool comprising:
a display;
a barcode scanner configured to detect a barcode associated with a position of interest, wherein the barcode is detected based on imaging by the barcode scanner;
a position detection module; and
a processor coupled to the display, the position detection module and the barcode scanner, the processor configured to:
decode the barcode to extract data associated with the position of interest, the data including position data for the position of interest;
control a surveying measurement by the position detection module to determine position data for the position of interest; and
present decoded data including an angular position of the surveying tool with respect to the position of interest, to the display of the surveying tool, wherein the processor controls display of position data decoded from the barcode and display of position data determined from the surveying measurement.
12. The surveying tool of claim 11, wherein the barcode is one of a two-dimensional barcode, and a matrix code.
13. The surveying tool of claim 11, wherein the data extracted from the barcode relates to one of an elevation, and grade.
14. The surveying tool of claim 11, wherein the feature information for the position of interest includes at least one of a date of last entry, a longitude, a latitude, a location of a utility service, a location of a next position of interest, and user-provided data.
15. The surveying tool of claim 11, wherein presenting the decoded data includes displaying the decoded data on the display, the decoded data associated with a barcode target identified by a target window displayed by the surveying tool.
16. The surveying tool of claim 11, wherein the processor is further configured to determine a surveying measurement for the position of interest based on orientation data decoded from the barcode.
17. The surveying tool of claim 16, wherein the processor is further configured to evaluate a change in the position of interest based, at least in part, on the decoded data and surveying measurement, wherein evaluating a characteristic of the position of interest includes comparing a result of the surveying measurement to data extracted from the barcode.
18. The surveying tool of claim 11, wherein the processor is further configured to update data provided by the barcode, wherein updating comprises generating an updated barcode to be labeled to the position of interest, the updated barcode having data encoded for the position of interest.
19. The surveying tool of claim 11, further comprising a barcode printer configured to print barcodes containing the position and the elevation of the position of interest.
20. The surveying tool of claim 11, further comprising labeling the position of interest with the barcode bearing the encoded data.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A self-contained tremolo device for a hollow bodied stringed musical instrument, said device comprising:
a movable bridge positioned on a hollow bodied stringed musical instrument and adapted to hold strings of said musical instrument in tension, and including crescent shaped notches that line up with respective pivot posts;
an adjustment device adapted to engage said bridge and to move said bridge to change said tension of said strings; and
a support frame disposed within the hollow bodied stringed musical instrument and having two top parallel support members having first ends joined to first ends of two rear vertical support members having second ends joined to first ends of two bottom parallel support members, with a U-shaped brace member joined to second ends of the top parallel support members and second ends of the bottom parallel support members, and with a U-shaped central cross member having said pivot posts disposed thereon and joined to a central portion of the top parallel support members and the bottom parallel support members,
wherein the support frame is adapted to engage said bridge at a first area and to engage said musical instrument at a second area,
wherein said support frame receives a force from said bridge over said first area and transmits said force to said musical instrument over said second area, and
wherein said second area is greater than said first area.
2. The tremolo device according to claim 1, wherein said musical instrument is an acoustic guitar.
3. The tremolo device according to claim 1, wherein said musical instrument has an internal cavity, and wherein said support frame is positioned in said internal cavity.
4. The tremolo device according to claim 3, wherein said second area of said support frame engages more than one interior wall of said internal cavity.
5. The tremolo device according to claim 3, wherein said support frame is adapted to transmit vibration throughout said internal cavity.
6. The tremolo device according to claim 1, wherein said support frame is wood.
7. The tremolo device according to claim 1, wherein said support frame is rectangular.
8. The tremolo device according to claim 7, wherein said first area is located on a single beam of said support frame, and wherein said second area is located on more than one beam of said support frame.
9. The tremolo device according to claim 1, wherein said movable bridge comprises a wooden surface with an underlying metal plate.
10. The tremolo device according to claim 1, wherein said musical instrument has an internal cavity, and wherein said movable bridge is positioned over a body opening in said musical instrument.
11. The tremolo device according to claim 10, wherein a first edge of said movable bridge pivots at a side of said body opening and a second edge of said movable bridge moves in and out of said internal cavity to change said tension of said strings.
12. The tremolo device according to claim 11, wherein said movable bridge pivots at one or more pivot posts.
13. The tremolo device according to claim 12, wherein said one or more pivot posts engage said support frame at said first area.
14. The tremolo device according to claim 1, further comprising springs adapted to be attached between said support frame and said movable bridge.
15. The tremolo device according to claim 1, wherein said central cross member includes a recessed cavity for said movable bridge and is adapted to support a hollow body of said musical instrument.