1-85. (canceled)
86. A method for sorting cells comprising:
combining a sample having a plurality of target cells with a plurality of modular polynucleotide-encoded capture agent sets, each capture agent comprising a tetramer of an antigen-presenting MHC attached to an optimized streptavidin scaffold molecule and an encoding polynucleotide attached to the scaffold molecule, wherein the MHC specifically binds to a predetermined target cell and the encoding polynucleotide specifically binds to a complementary substrate polynucleotide and wherein each set of capture agents binds the same target cells and binds the same complementary substrate polynucleotides;
forming target cell complexes of capture agents bound to the target cells;
combining the target cell complexes with a plurality of complementary substrate polynucleotide sets attached to a substrate; and
hybridizing the encoding polynucleotides to complementary substrate polynucleotides to sort target cell complexes.
87. The method of claim 86, wherein each capture agent has three encoding polynucleotides attached to the scaffold molecule.
88. The method of claim 86 further comprising a plurality of sets of capture agents, wherein each set of capture agents has encoding polynucleotides orthogonal to the encoding polynucleotides of each other set of capture agents and an MHC tetramer that binds to a target cell surface antigen different from the target cell surface antigen to which the other sets of MHC tetramers bind.
89. The method of claim 88, wherein the encoding polynucleotides of each set of capture agents encodes a restriction site different from the restriction enzyme site of the encoding polynucleotides of the other sets of capture agents.
90. The method of claim 89, wherein substrate bound target cells complexes are combined with restriction enzymes.
91. The method of claim 86, wherein the encoding polynucleotides are ssDNA oligomers.
92. The method of claim 86, wherein the capture agent further comprises a labeled molecule.
93. The method of claim 92, wherein the labeled molecule is selected from the group consisting of radioactive isotopes, fluorophores, chemiluminescent dyes, chromophores, enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, dyes, metal ions, nanoparticles, metal sols, and ligands.
94. The method of claim 93 further comprising detecting a labeling signal.
95. The method of claim 86, wherein the MHC is an MHC class I, II or III protein.
96. The method of claim 95, wherein the MHC binds a T cell receptor.
97. The method of claim 86, wherein the target cells are selected from the group consisting of CD8, CD4, CD3 or antigen specific T cells.
98. The method of claim 97, wherein the T cell antigen is selected from the group consisting of MART-1, OVA, CMV, and tyrosinase.
99. The method of claim 86, wherein the optimized streptavidin scaffold molecule further comprises an amino acid sequence as set forth in SEQ ID NO:2.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Motor vehicle comprising:
an electric seat adjuster for a seat, and
an occupant protection system comprising a belt assigned to the seat, which belt, in a buckled condition, restrains an occupant in an event of a vehicle impact,
wherein, as a function of the buckling condition of the belt, an adjusting range of the seat adjuster is limited to a narrow adjusting range if the belt is not buckled and is limited to a wider adjusting range if the belt is buckled.
2. Motor vehicle according to claim 1, wherein, if, at a start of the motor vehicle, the belt is not buckled and the seat is adjusted in a position outside the narrow adjusting range, the seat is automatically moved into a position within the narrow adjusting range andor a warning signal is emitted.
3. Motor vehicle according to claim 1, wherein, if the belt is opened up during the driving operation and thus the buckling condition changes from \u201cbelt is buckled\u201d to \u201cbelt is not buckled\u201d, the seat automatically moves from a position outside the narrow adjusting range into a position within the narrow adjusting range andor a warning signal is emitted.
4. Motor vehicle according to claim 2, wherein the seat can be moved from a position outside the narrow adjusting range to a position within the narrow adjusting range only above a predefined minimal speed of the motor vehicle.
5. Motor vehicle according to claim 3, wherein the seat can be moved from a position outside the narrow adjusting range to a position within the narrow adjusting range only above a predefined minimal speed of the motor vehicle.
6. Motor vehicle according to claim 2, wherein the seat is moved so slowly into the narrow adjusting range that the moving of the seat is perceived by the occupant as not being disturbing or is almost not perceived.
7. Motor vehicle according to claim 3, wherein the seat is moved so slowly into the narrow adjusting range that the moving of the seat is perceived by the occupant as not being disturbing or is almost not perceived.
8. Motor vehicle according to claim 2, wherein the moving of the seat is acoustically, haptically or visually indicated to the occupant.
9. Motor vehicle according to claim 3, wherein the moving of the seat is acoustically, haptically or visually indicated to the occupant.
10. Motor vehicle according to claim 1, wherein a narrow adjusting range and a wide adjusting range are provided for at least one electrically adjustable component of the seat.
11. Motor vehicle according to claim 2, wherein a narrow adjusting range and a wide adjusting range are provided for at least one electrically adjustable component of the seat.
12. Motor vehicle according to claim 1, wherein the seat is automatically moved into a position within the narrow adjusting range after the stoppage of the motor vehicle and after the occupant has left the motor vehicle.
13. A vehicle seat assembly comprising:
a vehicle seat,
a seat belt assigned to the seat and operable in a buckled condition to restrain a seat occupant during a vehicle impact event,
a seat adjuster, and
a seat adjuster controller operable to control an adjusting range of the seat adjuster as a function of whether the seat belt is buckled or unbuckled.
14. An assembly according to claim 13, wherein the seat adjuster controller is operable to change the adjusting range between a predetermined narrow range and a predetermined wider range.
15. An assembly according to claim 14, wherein the seat adjuster is operable to adjust a spacing between a vehicle airbag and the seat, and
wherein the seat adjuster changes to the narrow range when the belt is unbuckled.
16. An assembly according to claim 14, wherein the adjuster is operable to adjust a plurality of seat components,
wherein the seat adjuster is operable to adjust a spacing between a vehicle airbag and at least one of said seat components.
17. An assembly according to claim 16, wherein the seat adjuster changes to the narrow range when the belt is unbuckled.
18. An assembly according to claim 15, comprising warning means for providing a warning signal when the narrow range is selected by the seat adjuster controller.
19. An assembly according to claim 17, comprising warning means for providing a warning signal when the narrow range is selected by the seat adjuster controller.
20. An assembly according to claim 14, wherein, if the belt is opened up during the driving operation and thus the buckling condition changes from \u201cbelt is buckled\u201d to \u201cbelt is not buckled\u201d, the seat automatically moves from a position outside the narrow adjusting range into a position within the narrow adjusting range andor a warning signal is emitted.
21. An assembly according to claim 14, wherein the seat can be moved from a position outside the narrow adjusting range to a position within the narrow adjusting range only above a predefined minimal speed of the motor vehicle.
22. An assembly according to claim 14, wherein the seat is moved so slowly into the narrow adjusting range that the moving of the seat is perceived by the occupant as not being disturbing or is almost not perceived.
23. An assembly according to claim 14, wherein the moving of the seat is acoustically, haptically or visually indicated to the occupant.
24. An assembly according to claim 14, wherein the seat is automatically moved into a position within the narrow adjusting range after the stoppage of the motor vehicle and after the occupant has left the motor vehicle.
25. A method of operating a vehicle seat assembly comprising:
a vehicle seat,
a seat belt assigned to the seat an operable in a buckled condition to restrain a seat occupant during a vehicle impact event,
a seat adjuster, and
a seat adjuster controller operable to control an adjusting range of the seat adjuster as a function of whether the seat belt is buckled or unbuckled,
said method including automatically changing the adjusting range from a wider to a narrower adjusting range whenever the belt is unbuckled.