1. A roller bottle for use in culturing cells, comprising:
a bottom;
a top; and,
a continuous side wall extending between said bottom and said top, said side wall having an interior surface and an exterior surface, and being spaced about a longitudinal axis of said bottle for allowing said bottle to rotate along said side wall about the longitudinal axis for distributing, along said side wall, a liquid medium contained in said bottle to wet said side wall, said side wall including at least one pleat extending from a first point proximate said top to a first point proximate said bottom, said pleat being arranged at an angle offset with respect to a plane perpendicular to the longitudinal axis of said bottle, said pleat defining at least one interior channel on said inner surface of said side wall for permitting laminar flow and distribution of said liquid along said pleat, said interior channel providing a surface for culturing the cells, wherein at least one smooth land is formed in said side wall, said land interrupting said pleat.
2. The bottle of claim 1, wherein said angle is in the range of about 2\xb0 to about 70\xb0.
3. The bottle of claim 2, wherein said pleat is a single helical pleat.
4. The bottle of claim 3, wherein said angle is in the range of about 2\xb0 to about 10\xb0.
5. The bottle of claim 2, wherein said at least one pleat comprises a plurality of substantially parallel pleats.
6. The bottle of claim 5, wherein said angle is in the range of about 40\xb0 to about 70\xb0.
7. The bottle of claim 6, wherein said angle is approximately 65\xb0.
8. The bottle of claim 1, wherein said land runs between a second point proximate said top and a second point proximate said bottom.
9. The bottle of claim 8, wherein said land is substantially arcuate, and lies in a cylindrical plane of generally constant distance from the longitudinal axis of said bottle.
10. The bottle of claim 9, wherein said exterior surface of said pleat includes apices extending to a generally cylindrical plane co-incident with said plane of said land.
11. The bottle of claim 1, wherein said pleat is approximately 0.079 inches deep.
12. The bottle of claim 1, wherein said interior channel has a substantially uniform width.
13. The bottle of claim 12, wherein said interior channel is approximately 0.195 inches wide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A transgenic non-human animal comprising a recombinant nucleic acid molecule stably integrated into the genome of said animal, said molecule encoding a light-generating fusion protein comprising a ligand binding site and a light-generating polypeptide moiety, wherein said ligand binding site comprises a polypeptide selected from the group consisting of (a) a polypeptide comprising SEQ ID NO: 9; (b) polypeptide comprising amino acids 556 to 575 of SEQ ID NO: 9; (c) a polypeptide comprising amino acids 530\u2013625 of SEQ ID NO: 9.
2. The non-human animal of claim 1, whereupon ligand binding to said ligand binding site alters the luminescence of said light-generating polypeptide moiety.
3. The non-human animal of claim 1, wherein said recombinant nucleic acid molecule is operably linked to one or more regulatory sequences.
4. The non-human animal of claim 3, wherein said recombinant nucleic acid molecule is operably linked promoter.
5. The non-human animal of claim 1, wherein said recombinant nucleic acid molecule is heterologous to said non-human animal.
6. The non-human animal of claim 1, wherein said light-generating polypeptide moiety is selected from the group consisting of ferredoxin IV, green fluorescent protein, red fluorescent protein, yellow fluorescent protein, blue fluorescent protein, the luciferase family and the aequorin family.
7. An cell isolated from the non-human animal of claim 1.
8. The cell of claim 7, wherein said cell is a stem cell or a germ cell.
9. A method for the production of a transgenic non-human animal, comprising introduction of a recombinant nucleic acid molecule into a germ cell, an embryonic cell, or an egg cell, said recombinant nucleic acid molecule encoding a light-generating fusion protein comprising a ligand binding site and a light-generating polypeptide moiety, wherein said ligand binding site comprises a polypeptide selected from the group consisting of (a) a polypeptide comprising SEO ID NO: 9: (b) polypeptide comprising amino acids 556 to 575 of SEO ID NO: 9; (c) a polypeptide comprising amino acids 530\u2013625 of SEO ID NO: 9.
10. The method of claim 9, wherein said non-human animal is a rodent.
11. The method of claim 10, wherein said rodent is a mouse.