1. A polypeptide which comprises the amino acid sequence represented by SEQ ID NO:2 or 4, and binds to CAP; or a polypeptide which consists of an amino acid sequence in which 1 to 10 amino acids are deleted, substituted, andor inserted in the amino acid sequence represented by SEQ ID NO:2 or 4, and binds to CAP.
2. A polypeptide which consists of an amino acid sequence having a homology of 90% or more with the amino acid sequence represented by SEQ ID NO:2 or 4, and is a protein which binds to CAP.
3. A polypeptide consisting of the amino acid sequence represented by SEQ ID NO:2 or 4.
4. A polynucleotide encoding the polypeptide according to any one of claims 1 to 3.
5. An expression vector comprising the polynucleotide according to claim 4.
6. A cell transformed with the expression vector according to claim 5.
7. A method for screening a binding inhibitor between the polypeptide according to any one of claims 1 to 3 and CAP, which comprises:
allowing the polypeptide to contact with a test substance,
measuring change of binding between the polypeptide and CAP, and
selecting a substance which inhibits the binding.
8. The method for screening according to claim 7, wherein the binding inhibitor is an agent for improving insulin resistance andor an agent for improving glucose metabolism.
9. A method for screening an agent for improving insulin resistance andor an agent for improving glucose metabolism, which comprises:
allowing a cell which expresses the polypeptide according to any one of claims 1 to 3 to contact with a test substance, and
measuring change of the expression amount of the polypeptide.
10. A method for producing a pharmaceutical composition for improving insulin resistance andor improving glucose metabolism, which comprises:
carrying out screening with the method for screening according to any one of claims 7 to 9, and
carrying out formulation using the substance obtained by the screening.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of correlating a two dimensional representation of a body to a three dimensional representation of the body using a computer comprising:
i. acquiring a two dimensional representation of the body;
ii. generating, by the computer, a fitness function for the two dimensional representation;
iii. determining, by the computer, two dimensional outlines of the three dimensional representation of the body for a plurality of sets of three dimensional position and orientation values, the two dimensional outline of the three dimensional body is determined using ray tracing from the origin of the position and orientation values, the origin being outside of the three dimensional representation;
iv. comparing, by the computer, the two dimensional outlines to the fitness function to generate measures of fit for each set of three dimensional position and orientation values;
v. breeding, by the computer, new sets of position and orientation values using a genetic algorithm; and
vi. repeating steps iii to v until the measure of fit converges to an acceptable solution where the two dimensional representation of the body is correlated to the three dimensional representation of the body.
2. A method as claimed in claim 1 wherein the fitness function is based on the outline of the acquired two dimensional representation.
3. A method as claimed in claim 2 wherein the fitness function is related to distance from the outline of the acquired two dimensional representation.
4. A method as claimed in claim 1 wherein ray tracing is performed around the perimeter of the three dimensional representation to generate a two dimensional outline of the three dimensional representation.
5. A method as claimed in claim 4 wherein the first pixel of the two dimensional outline is obtained by moving outwardly from within the three dimensional representation to an edge of the model.
6. A method as claimed in claim 5 wherein subsequent pixels of the two dimensional array are determined by ray tracing adjacent pixels to the last pixel determined.
7. A method as claimed in claim 1 wherein the position of the three dimensional representation is determined by x, y, z values and the orientation is determined by xr, yr, zr values.
8. A method as claimed in claim 7 wherein a plurality of sets of x, y, z, xr, yr and zr values are randomly generated.
9. A method as claimed in claim 5 wherein a plurality of sets of x, y, z, xr and zr values are selected from random values within given ranges.
10. A method as claimed in claim 1 wherein a plurality of two dimensional representations are acquired and used to create the fitness function.
11. A method as claimed in claim 1 wherein a string is generated from which the plurality of sets of three dimensional position and orientation values are generated.
12. A method as claimed in claim 11 wherein the string is generated using a random number generator.
13. A method as claimed in claim 1 wherein in the breeding step sets of position and orientation values are sorted from fittest to poorest based on comparison with the fitness function.
14. A method as claimed in claim 13 wherein the fittest position and orientation values are selected in the breeding step.
15. A method as claimed in claim 13 wherein a proportion of the fittest position and orientation values and a proportion of random values are selected in the breeding step.
16. A method as claimed in claim 13 wherein the position and orientation values are selected randomly in the breeding step with selection weighted towards the fittest values.
17. A method as claimed in claim 13 wherein a proportion of the best fitting position and orientation values are selected in the breeding step.
18. A method as claimed in claim 17 wherein a proportion of the less fit values are selected in the breeding step.
19. A method as claimed in claim 17 wherein the remaining values are selected randomly.
20. A method as claimed in claim 1 wherein an acceptable solution is achieved when the position and orientation values are stable for several iterations.
21. A method as claimed in claim 1 wherein an acceptable solution is achieved when an allowed amount computational processing has been expended.
22. A method as claimed in claim 1 wherein mutation of the position and orientation values is increased if the measure of fit remains below a threshold level for a prescribed period.
23. A method as claimed in claim 1 wherein some of the fittest position and orientation values are used in each successive iteration.
24. A method as claimed in claim 1 wherein the correlated representations are compared to assess wear.
25. A non-transitory computer-readable storage medium with an executable program stored thereon, wherein the program instructs a computer to perform the method of claim 1.
26. A method as claimed in claim 1 wherein the body to be correlated is a prosthesis.
27. A method as claimed in claim 26 wherein the three dimensional representation of the prosthesis is of the prosthesis at an earlier time period than the two dimensional representation and wherein the method includes the step of displaying the two dimensional representation and the correlated two dimensional representation of the earlier three dimensional representation to a user for the user to assess wear.
28. A method as claimed in claim 27 wherein the two dimensional representation is acquired from a capture device selected from the group comprising a CT scanner, a x-ray machine and one or more cameras and wherein the three dimensional representation is a virtual model of the prosthesis as manufactured.
29. A method as claimed in claim 26 wherein the three dimensional representation of the prosthesis is of the prosthesis at an earlier time period than the two dimensional representation and wherein the method includes the step of accessing wear of the prosthesis by comparing the latter two dimensional representation to the correlated two dimensional representation of the earlier three dimensional representation.
30. A method as claimed in claim 29 wherein the two dimensional representation is acquired from a capture device selected from the group comprising a CT scanner, a x-ray machine and one or more cameras and wherein the three dimensional representation is a virtual model of the prosthesis as manufactured.
31. A computer system for correlating a two dimensional representation of a body to a three dimensional representation of the body comprising:
a. input means for acquiring a two dimensional representation of the body; and
b. processor that:
i. generates a fitness function for the two dimensional representation;
ii. determines a two dimensional outline of the three dimensional representation of the body for a first set of three dimensional position and orientation values, the two dimensional outline of the three dimensional body is determined using ray tracing from the origin of the position and orientation values, the origin being outside of the three dimensional representation;
iii. compares the two dimensional outline to the fitness function to generate a measure of fit;
iv. breeds new position and orientation values using a genetic algorithm; and
v. repeats steps iii to v until the measure of fit converges to an acceptable solution where the two dimensional representation of the body is correlated to the three dimensional representation of the body.
32. A system as claimed in claim 31 including a two dimensional image capture device to capture the two dimensional representation.
33. A system as claimed in claim 31 wherein the two dimensional image capture device is an x-ray machine.
34. A system as claimed in claim 31 wherein the two dimensional image capture device is one or more camera.
35. A system as claimed in claim 31 wherein the two dimensional image capture device is a stereoscopic vision system.
36. A system as claimed in claim 31 wherein the two dimensional image capture device is an ultrasonic imaging device.
37. A method of correlating a first representation of an object to a second representation of the object using a computer comprising:
i. acquiring a first representation of the object;
ii. generating, by the computer, a fitness function for the first representation;
iii. for a first set of position and orientation values determining an outline of the second representation of the object, the two dimensional outline of the second representation of the object is determined using ray tracing from the origin of the position and orientation values, the origin being outside of the second representation of the object;
iv. comparing, by the computer, the outline to the fitness function to generate a measure of fit;
v. generating, by the computer, new position and orientation values based on the first set of position and orientation values and the measure of fit; and
vi. repeating steps iii to v until the measure of fit converges to an acceptable solution where the first representation of the object is correlated to the second dimensional representation of the object.