1. A method comprising:
maintaining a biotin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers separately from an avidin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers;
simultaneously applying the biotin-containing component and avidin-containing component directly to tissue; and
allowing biotin groups on the biotin-containing component to react with avidin groups on the avidin-containing component to form a three-dimensional crosslinked matrix as a tissue adhesive or sealant,
wherein the absorbable polymer of the biotin-containing component or the avidin-containing component is selected from the group consisting of polycaprolactone, poly-D,L-lactic acid, poly-L-lactic acid, poly(lactide-co-glycolide), poly(hydroxybutyrate), poly(hydroxybutyrate-co-valerate), polydioxanone, polyorthoester, polyanhydride, poly(glycolic acid), poly(glycolic acid-cotrimethylene carbonate), polyphosphoester, polyphosphoester urethane, polyglutamic acid, polyaspartic acid, absorbable cyanoacrylates, poly(trimethylene carbonate), copoly(ether-esters), polyalkylene oxalates, polyphosphazenes, polyiminocarbonates, aliphatic polycarbonates, and combinations thereof.
2. The method according to claim 1, wherein the biotin-containing component comprises a branched polymer functionalized with biotin.
3. The method according to claim 1, wherein the biotin-containing component comprises polyethylene glycol having a molecular weight of less than 1000.
4. The method according to claim 1, wherein the avidin-containing component comprises a polymer functionalized with avidin.
5. The method according to claim 1, wherein the avidin-containing component comprises a polymer functionalized with streptavidin.
6. The method according to claim 1, wherein the avidin-containing component comprises a branched polymer.
7. The method according to claim 1, wherein the avidin-containing component comprises a polyethylene glycol having a molecular weight of less than 1000.
8. The method according to claim 1, wherein the three-dimensional crosslinked matrix further comprises a bioactive agent.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for cyclically preparing monomer boron and coproducing potassium cryolite using potassium fluoborate as an intermediate material, including the following steps:
A) adding hydrofluoric acid to boric acid or boron oxide to enable a reaction at a temperature of between 100 and 200 DEG C. to form fluoboric acid;
B) adding a potassium sulphate aqueous solution to the fluoboric acid to enable a reaction to form a potassium fluoborate precipitate, centrifuging and rinsing the potassium fluoborate precipitate to obtain the intermediate material potassium fluoborate;
C) putting dried potassium fluoborate into a reactor, injecting an inert gas to the reactor after vacuumizing, heating the reactor to a temperature of between 700 and 800 DEG C., adding aluminium in the reactor and stirring quickly to enable a reaction for 4 to 6 hours to form the monomer boron and potassium cryolite, or, putting the aluminium into the reactor, injecting an inert gas to the reactor after vacuumizing, heating the reactor to the temperature of between 700 and 800 DEG C., adding dried and flowable potassium fluoborate in the reactor and stirring quickly to enable a reaction for 4 to 6 hours to form the monomer boron and potassium cryolite;
D) extracting molten liquid potassium cryolite; after the molten liquid potassium cryolite is cooled, crushing and sending it to a rotary reaction kettle quantificationally together with concentrated sulphuric acid to enable a reaction at a temperature of between 400 and 500 DEG C. to form hydrogen fluoride gas and aluminium potassium sulphate, potassium sulphate; collecting the hydrogen fluoride gas and dissolving it into water to obtain a hydrofluoric acid aqueous solution; reacting the aluminium potassium sulphate with an aqueous solution of potassium hydroxide after crushing the aluminium potassium sulphate, and obtaining the aqueous solution of potassium sulphate after separating out the solid aluminium hydroxide; and
E) recycling the obtained hydrofluoric acid aqueous solution and the aqueous solution of potassium sulphate to the front end to leach the boric acid or boron oxide, so as to achieve the purpose of cyclically preparing the intermediate material potassium fluoborate.
2. The method for cyclically preparing monomer boron and coproducing potassium cryolite using potassium fluoborate as an intermediate material according to claim 1, wherein in Step C, the aluminium is molten aluminium which is added in the reactor in a dripping way or the dried and flowable potassium fluoborate is added in the reactor in a measurable flowing way.
3. The method for cyclically preparing monomer boron and coproducing potassium cryolite using potassium fluoborate as an intermediate material according to claim 1, wherein in Step C, the inert gas is argon.