1. A multi-conjugate adaptive optics system for reducing aberration-induced fluctuations in a beam, the system comprising:
a first wavefront corrector configured to correct a wavefront of a beam;
a first wavefront sensor configured to measure the wavefront of the beam corrected by said first wavefront corrector, wherein said first wavefront sensor and said first wavefront corrector are conjugated to a first plane;
a second wavefront corrector configured to correct the wavefront of the beam corrected by said first wavefront corrector; and
a second wavefront sensor configured to measure the wavefront of the beam corrected by said second wavefront corrector, wherein said second wavefront sensor and said second wavefront corrector are conjugated to a second plane separated from the first plane,
wherein said first wavefront corrector is configured to correct the wavefront based on the measurement of said first wavefront sensor, and said second wavefront corrector is configured to correct the wavefront based on the measurement of said second wavefront sensor.
2. The multi-conjugate adaptive optics system according to claim 1, wherein said first wavefront corrector and said second wavefront corrector each comprises a deformable mirror.
3. The multi-conjugate adaptive optics system according to claim 2, wherein the deformable mirror is segmented.
4. The multi-conjugate adaptive optics system according to claim 1, wherein said first wavefront sensor and said second wavefront sensor each comprises a phase-shifting, point-diffraction interferometer.
5. The multi-conjugate adaptive optics system according to claim 1, wherein said first wavefront corrector is configured to null the phase of the wavefront based on the measurement of said first wavefront sensor and independent of the measurement of said second wavefront sensor, and said second wavefront corrector is configured to null the phase of the wavefront based on the measurement of said second wavefront sensor and independent of the measurement of said first wavefront sensor.
6. The multi-conjugate adaptive optics system according to claim 1, wherein the scaled optical path length separating the first and second planes is approximately equal to or greater than the optical path length between an aberration in the beam path and the multi-conjugate adaptive optics system.
7. A method for reducing turbulence-induced fluctuations in a beam, the method comprising the steps of:
correcting a wavefront of a beam with a first wavefront corrector;
measuring the wavefront of the beam corrected with the first wavefront corrector with a first wavefront sensor, wherein the first wavefront corrector and the first wavefront sensor are conjugated to a first plane;
correcting the wavefront of the beam corrected with the first wavefront corrector with a second wavefront corrector; and
measuring the wavefront of the beam corrected with the second wavefront corrector with a second wavefront sensor, wherein the second wavefront corrector and the second wavefront sensor are conjugated to a second plane separated from the first plane,
wherein the first correcting step comprises correcting the wavefront based on the measurement obtained in the first detecting step, and the second correcting step comprises correcting the wavefront based on the measurement obtained in the second detecting step.
8. The method according to claim 7, wherein said first correcting step comprises nulling the phase of the wavefront of the beam independent of said second correcting step and said second correcting step comprises nulling the phase of the wavefront of the beam independent of said first correcting step.
9. The method according to claim 7, wherein the scaled optical path length separating the first and second planes is approximately equal to or greater than the optical path length between an aberration in the beam path and the multi-conjugate adaptive optics system.
10. A multi-conjugate adaptive optics system for reducing turbulence-induced fluctuations in a beam, the system comprising:
a first wavefront corrector configured to correct a wavefront of an incident beam;
a first wavefront sensor configured to measure the wavefront of the incident beam corrected by said first wavefront corrector, wherein said first wavefront corrector and said first wavefront sensor are conjugated to a first plane;
a second wavefront corrector configured to correct a wavefront of the incident beam corrected by said first wavefront corrector;
a second wavefront sensor configured to measure the wavefront of the incident beam corrected by said second wavefront corrector, wherein said second wavefront corrector and said second wavefront sensor are conjugated to a second plane; and
a third sensor configured to measure a wavefront of a probe beam corrected by said second wavefront corrector, wherein said third sensor is conjugated to a third plane and the optical path length between the first and second planes is equal to the optical path length between the second and third planes, and
wherein said first wavefront corrector is configured to correct the wavefront based on a combination of the measurements of said first wavefront sensor and said third wavefront sensor, and said second wavefront corrector is configured to correct the wavefront based on the measurement of said second wavefront sensor.
11. The multi-conjugate adaptive optics system according to claim 10, wherein said first wavefront corrector and said second wavefront corrector each comprise a deformable mirror.
12. The multi-conjugate adaptive optics system according to claim 11, wherein the deformable mirror is a segmented deformable mirror.
13. The multi-conjugate adaptive optics system according to claim 10, wherein said first wavefront sensor, said second wavefront sensor and said third sensor each comprises a phase-shifting, point-diffraction interferometer.
14. The multi-conjugate adaptive optics system according to claim 10, wherein said first wavefront corrector is configured to null the phase of the wavefront based on the measurements of said first and third sensors and independent of the measurement of said second sensor, and said second wavefront corrector is configured to null the phase of the wavefront based on the measurement of said second wavefront sensor and independent of the measurements of said first and third sensors.
15. The multi-conjugate adaptive optics system according to claim 10, wherein said third sensor is conjugated to the first plane and the probe beam propagates along a common path of the incident beam in the opposite direction of the incident beam within the system.
16. The multi-conjugate adaptive optics system according to claim 10, wherein the scaled optical path length separating the first and second planes is approximately equal to or greater than the optical path length between an aberration in the beam path and the multi-conjugate adaptive optics system.
17. A method for reducing turbulence-induced fluctuations in a beam, the method comprising the steps of:
correcting a wavefront of an incident beam with a first wavefront corrector;
measuring the wavefront of the incident beam corrected with the first wavefront corrector with a first wavefront sensor, wherein the first wavefront corrector and the first wavefront sensor are conjugated to a first plane;
correcting the wavefront of the incident beam corrected with the first wavefront corrector with a second wavefront corrector;
measuring the wavefront of the incident beam corrected with the second wavefront corrector with a second wavefront sensor, wherein the second wavefront corrector and the second wavefront sensor are conjugated to a second plane separated from the first plane; and
measuring a wavefront of a probe beam corrected with the second wavefront corrector with a third wavefront sensor, wherein the third wavefront sensor is conjugated to a third plane and the optical path length between the first and second planes is equal to the optical path length between the second and third planes,
wherein the first correcting step comprises correcting the wavefront based on a combination of the measurements obtained in the first and third measuring steps, and the second correcting step comprises correcting the wavefront based on the measurement obtained in the second measuring step.
18. The method according to claim 17, wherein said first correcting step comprises nulling the wavefront of the incident beam independent of said second correcting step and said second correcting step comprises nulling the wavefront of the incident beam independent of said first correcting step.
19. The method according to claim 17, wherein the third sensor is conjugated to the first plane and the probe beam propagates along a common path of the incident beam in the opposite direction of the incident beam within the system.
20. The method according to claim 17, wherein the scaled optical path length separating the first and second planes is approximately equal to or greater than the optical path length between an aberration in the beam path and the multi-conjugate adaptive optics system.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A peptide-polysaccharide conjugate comprising at least one peptide linked with a polysaccharide, peptide being a defensin and said peptide being capable of exerting antimicrobial activity.
2. A method according to claim 1, wherein said defensin comprises at least one peptide based on at least a portion of the amino acid sequence of human \u03b2-defensin-3, said portion being the last 10-14 residues of said amino acid sequence.
3. A peptide-polysaccharide conjugate according to claim 2, wherein said peptide has a partial amino acid sequence comprising KSSTRGRKSSRRKK (SEQ ID NO. 2).
4. A peptide-polysaccharide conjugate according to claim 2, wherein said peptide has a partial amino acid sequence comprising RGRKSSRRKK (SEQ ID NO. 3).
5. A peptide-polysaccharide conjugate according to claim 2, wherein said peptide has a partial amino acid sequence comprising RGRRSSRRKK (SEQ ID NO. 4) and an amide group located on the C-terminus.
6. A peptide-polysaccharide conjugate according to claim 2, wherein the site of attachment of said peptide for said polysaccharide comprises at least one of the following:
a. the \u03b5-amino group of lysine of said peptide; and
b. the \u03b1-amino group of the N-terminus of said peptide.
7. A peptide-polysaccharide conjugate according to claim 1, wherein said polysaccharide is cellulose.
8. A peptide-polysaccharide conjugate according to claim 1, wherein said polysaccharide is agarose.
9. A peptide-polysaccharide conjugate according to claim 1, wherein said polysaccharide is functionalized via at least one R group prior to linking with said peptide, said at least one R group including:
a. aldehyde;
b. amine;
c. carboxylic acid;
d. hydroxyl;
e. ester;
f. thiol;
g. halide;
h. halide-equivalent; and
i. epoxide.
10. A peptide-polysaccharide conjugate according to claim 9, wherein a reducing agent is used to stabilize the linkage formed between said peptide and said polysaccharide, said polysaccharide being functionalized with said at least one R group being said aldehyde.
11. A method of producing a peptide-polysaccharide conjugate comprising linking a peptide with a polysaccharide to generate said peptide-polysaccharide conjugate, said peptide being a defensin and said peptide being capable of exerting antimicrobial activity.
12. A method according to claim 11, wherein said defensin comprises at least one peptide based on at least a portion of the amino acid sequence of human \u03b2-defensin-3, said portion being the last 10-14 residues of said amino acid sequence.
13. A method according to claim 12, wherein said peptide has a partial amino acid sequence comprising KSSTRGRKSSRRKK (SEQ ID NO. 2).
14. A method according to claim 12, wherein said peptide has a partial amino acid sequence comprising RGRKSSRRKK (SEQ ID NO. 3).
15. A method according to claim 12, wherein said peptide has a partial amino acid sequence comprising RGRRSSRRKK (SEQ ID NO. 4) and an amide group located on the C-terminus.
16. A method according to claim 12, wherein the site of attachment of said peptide for said polysaccharide comprises at least one of the following:
a. the \u03b5-amino group of lysine of said peptide; and
b. the \u03b1-amino group of the N-terminus of said peptide.
17. A method according to claim 11, wherein said polysaccharide is cellulose.
18. A method according to claim 11, wherein said polysaccharide is agarose.
19. A method according to claim 11, further including functionalizing said polysaccharide via at least one R group prior to linking with said peptide, said at least one R group including:
i. aldehyde;
ii. amine;
iii. carboxylic acid;
iv. hydroxyl;
v. ester;
vi. thiol;
vii. halide;
viii. halide-equivalent; and
ix. epoxide.
20. A method according to claim 19, further including adding a reducing agent to said peptide-polysaccharide conjugate for stabilizing the linkage formed between said peptide and said polysaccharide, said polysaccharide being functionalized with said at least one R group being said aldehyde.