What is claimed is:
1. An isolated polynucleotide comprising the nucleotide sequence set forth in SEQ ID NO:1.
2. The isolated polynucleotide of claim 1 wherein the polynucleotide consists of a nucleotide sequence of the formula
(R1)m-SEQ ID NO:1-(R2)n
wherein R1 and R2 are independently any nucleic acid residue, and m and n are each integers between 1 and 1000.
3. The isolated polynucleotide of claim 2 wherein the polynucleotide consists of the nucleotide sequence set forth in SEQ ID NO:1.
4. An isolated polynucleotide that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.
5. The isolated polynucleotide of claim 4 wherein the polynucleotide encodes the amino acid sequence set forth in SEQ ID NO:2.
6. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.
7. The isolated polypeptide of claim 6 wherein the polypeptide consists of an amino acid sequence of the formula
(R1)m-SEQ ID NO:2-(R2)n
wherein, at the amino terminus, R1 and R2 are independently any amino acid residue, and m and n are each integers between 1 and 1000.
8. The isolated polypeptide of claim 6 consisting of the amino acid sequence set forth in SEQ ID NO:2.
9. An expression vector comprising the isolated polynucleotide of claim 4 when said expression vector is present in a compatible host cell.
10. An isolated host cell comprising the expression vector of claim 9.
11. A process for producing a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2 comprising culturing the host cell of claim 10 and recovering the polypeptide from the culture.
12. A membrane of the host cell of claim 10 expressing said polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Method of joining films made from at least one layer from the group comprising polyethylene, polypropylene polyamide and polyethylene terephthalate by welding, wherein the materials in the region of the contact layer are heated by ultrasound until they have at least softened and are actively laterally and vertically intermixed by continuing to apply ultrasonic vibrations.
2. Method as claimed in claim 1, wherein the depth of the intermixing zone measures between 30% and 70% of the total thickness of the film seam.
3. Method as claimed in claim 1, wherein at least one of the films to be joined comprises two layers, the layer facing the join being made from polyethylene and the layer facing away from the join being made from polypropylene.
4. Method as claimed in claim 1, wherein at least one of the films to be joined comprises two layers, the layer facing the join being made from polypropylene and the layer facing away from the join being made from polyamide or polyethylene terephthalate.
5. Method as claimed in claim 1, wherein at least one of the films to be joined is made from more than two layers.
6. Method as claimed in claim 1, wherein one surface is treated or coated with a view to reducing sealing ability, for example by a corona treatment or lacquering.
7. Method as claimed in claim 1, wherein ultrasound is homogeneously transmitted to the cross-section of the film seam via an essentially flat surface of a sonotrode.
8. Method as claimed in claim 1, wherein the working surface of the sonotrode which comes into contact with the film vibrates predominantly in a direction perpendicular to its surface.
9. Method as claimed in claim 7, wherein a co-operating die with at least one raised area is disposed on the side of the sonotrode lying opposite the join, which is pushed into the previously softened film material.
10. Method as claimed in claim 9, wherein the at least one raised area is elongate.
11. Method as claimed in claim 8, wherein the at least one raised area has a cross-section in the shape of a trapezium or trapezoid.
12. Method as claimed in claim 11, wherein the base angle of the at least one raised area lies within a range of between 20\xb0 and 90\xb0.
13. Method as claimed in claim 9, wherein the at least one raised area has interruptions distributed across its length.
14. Method as claimed in claim 8, wherein the film facing the co-operating die comprises at least two layers and the height of the at least one raised area is higher than the thickness of the layer facing the co-operating die.
15. Method as claimed in claim 9, wherein the co-operating die is provided with means for cooling it.
16. Method as claimed in claim 1, wherein the films or their layers facing the join have a thickness in a range of from 15 mm to 1 mm.
17. Method as claimed in claim 1, wherein the join is pressed and cooled to promote hardening.
18. Method as claimed in claim 1, wherein the films are fed through a production line from at least one roll in the form of a material web.
19. Method as claimed in claim 18, wherein thermal welds andor deformations are made in regions of the films before or after processing with ultrasound.
20. Method as claimed in claim 18, wherein means are provided on the production line for temporarily relieving the tension introduced into the material web as it is transported.