1. A transgenic plant, wherein the plant expresses a cationic peptide selected from the group consisting of CEMA and CEMA-related peptides.
2. The transgenic plant of claim 1, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit tree, orchids, carnation, lilies, rose, other flower, cacao, coffee, rubber tree, Douglas Fir, spruce, pine, other conifer, poplar, elm, other deciduous tree, turf grass, or lawn grass.
3. The transgenic plant of claim 1, wherein the transgenic plant is a potato plant.
4. The transgenic plant of claim 1, wherein the transgenic plant is a tobacco plant.
5. A method of generating at least one cationic peptide, the method comprising: propagating at least one transgenic plant of claim 1; and
isolating the cationic peptide, wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
6. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
growing the transgenic plant of claim 1; and
harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
7. The transgenic plant part of claim 6, wherein the plant part is selected from the group consisting of cut flowers, fruits, vegetables, and herbs.
8. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 1, and the second part comprises non-transgenic plant tissue.
9. The transgenic plant of claim 1, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
10. The transgenic plant of claim 1, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
11. The transgenic plant of claim 1, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
12. A transgenic plant, comprising a recombinant nucleic acid molecule, wherein the nucleic acid molecule encodes a fusion peptide having a formula P-C or P-E, wherein C is a CEMA peptide, E is a CEMA-related peptide, and P is an anionic pro-region peptide.
13. The transgenic plant of claim 12, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit trees, orchid, carnation, lilies, rose, and other flowers, cacao, coffee, and rubber trees, Douglas Fir, spruce, pine, other conifers, poplar, elm, other deciduous trees, turf grass or lawn grass.
14. A transgenic plant according to claim 12, wherein the transgenic plant is a potato plant.
15. A transgenic plant according claim 12, wherein the transgenic plant is a tobacco plant.
16. A method of generating at least one cationic peptide, the method comprising:
propagating at least one transgenic plant according to claim 12; and
isolating the cationic peptide wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
17. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
growing the transgenic plant of claim 12; and
harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
18. The transgenic plant of claim 17, wherein the plant part is a cut flower, fruit, vegetable, or herbs.
19. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 12, and the second part comprises non-transgenic plant tissue.
20. The transgenic plant of claim 12, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
21. The transgenic plant of claim 12, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
22. The transgenic plant of claim 12, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
23. A transgenic plant, comprising a recombinant nucleic acid molecule encoding a fusion peptide having a formula selected from the group consisting of P-S-C and P-S-E, wherein C is a CEMA peptide, E is a CEMA-related peptide, P is an anionic pro-region peptide, and S is a spacer peptide.
24. The transgenic plant of claim 23, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit trees, orchid, carnation, lilies, rose, and other flowers, cacao, coffee, and rubber trees, Douglas Fir, spruce, pine, other conifers, poplar, elm, other deciduous trees, turf grass or lawn grass.
25. The transgenic plant of claim 23, wherein the transgenic plant is a potato plant.
26. The transgenic plant of claim 23, wherein the transgenic plant is a tobacco plant.
27. A method of generating at least one cationic peptide, the method comprising: propagating at least one transgenic plant of claim 23; and
isolating the cationic peptide wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
28. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
(a) growing the transgenic plant of claim 23; and
(b) harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
29. The transgenic plant part of claim 28, wherein the plant part is selected from the group consisting of cut flowers, fruits, vegetables, and herbs.
30. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 23, and the second part comprises non-transgenic plant tissue.
31. The transgenic plant of claim 23, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
32. The transgenic plant of claim 23, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
33. The transgenic plant of claim 23, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
34. A transgenic plant, comprising a nucleic acid molecule encoding a peptide comprising an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO: 2 and SEQ ID NO: 4;
(b) amino acid sequences that differ from an amino acid sequence specified in (a) by one or more conservative amino acid substitutions; and
(c) amino acid sequences that share at least 40% sequence identity with an amino acid sequence specified in (a), wherein the peptide has CEMA biological activity.
35. The transgenic plant of claim 34, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit trees, orchid, carnation, lilies, rose, and other flowers, cacao, coffee, and rubber trees, Douglas Fir, spruce, pine, other conifers, poplar, elm, other deciduous trees, turf grass or lawn grass.
36. The transgenic plant of claim 35, wherein the transgenic plant is a potato plant.
37. The transgenic plant of claim 35, wherein the transgenic plant is a tobacco plant.
38. A method of generating at least one cationic peptide, the method comprising:
propagating at least one transgenic plant of claim 34; and
isolating the cationic peptide wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
39. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
(a) growing the transgenic plant of claim 34; and
(b) harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
40. The transgenic plant part of claim 39, wherein the plant part is selected from the group consisting of cut flowers, fruits, vegetables, and herbs.
41. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 34, and the second part comprises non-transgenic plant tissue.
42. The transgenic plant of claim 34, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
43. A transgenic plant, according to claim 34, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
44. The transgenic plant of claim 34, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
45. The transgenic plant of claim 34, wherein the peptide further comprises an anionic pro-region peptide operably linked to an N-terminus of the peptide.
46. The transgenic plant of claim 34, wherein the peptide further comprises an N-terminal extension.
47. The transgenic plant of claim 34, wherein the peptide further comprises an C-terminal extension.
48. A transgenic plant, comprising a recombinant nucleic acid molecule encoding a peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 2 and SEQ ID NO: 4.
49. A transgenic plant according to claim 48, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit trees, orchid, carnation, lilies, rose, and other flowers, cacao, coffee, and rubber trees, Douglas Fir, spruce, pine, other conifers, poplar, elm, other deciduous trees, turf grass or lawn grass.
50. The transgenic plant of claim 48, wherein the transgenic plant is a potato plant.
51. The transgenic plant of claim 48, wherein the transgenic plant is a tobacco plant.
52. A method of generating at least one cationic peptide, the method comprising: propagating at least one transgenic plant of claim 48; and
isolating the cationic peptide wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
53. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
(a) growing the transgenic plant of claim 48; and
(b) harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
54. The transgenic plant part of claim 53, wherein the plant part is selected from the group consisting of cut flowers, fruits, vegetables, and herbs.
55. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 48, and the second part comprises non-transgenic plant tissue.
56. The transgenic plant of claim 48, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
57. The transgenic plant of claim 48, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
58. The transgenic plant of claim 48, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
59. A method for producing a transgenic plant that expresses a terminally extended cationic peptide conferring broad-spectrum pathogen resistance and that is compatible with plant physiology, the method comprising:
(a) transforming a plant cell with a nucleic acid sequence that encodes an N-terminally extended cationic peptide;
(b) expressing the terminally extended cationic peptide in a plant; and
(c) determining that the plant expressing the terminally extended peptide is resistant to a broad-spectrum of pathogens.
60. The method of claim 59, wherein the N-terminal extension sequence is selected from the group consisting of SEQ ID NOS: 5, 6, 7, and 11.
61. A transgenic plant, produced by the method of claim 59.
62. A transgenic plant according to claim 61, wherein the transgenic plant is a potato, tobacco, maize, wheat, rice, barley, soybean, legume, rapecanola, alfalfa, flax, sunflower, safflower, brassica, cotton, flax, peanut, clover, lettuce, tomato, cucurbits, cassaya, carrot, radish, pea, lentil, cabbage, cauliflower, broccoli, Brussels sprout, peppers, other vegetable, citrus tree, apple tree, pear tree, peach tree, apricot tree, walnut tree, other fruit trees, orchid, carnation, lilies, rose, and other flowers, cacao, coffee, and rubber trees, Douglas Fir, spruce, pine, other conifers, poplar, elm, other deciduous trees, turf grass or lawn grass.
63. The transgenic plant of claim 61, wherein the transgenic plant is a potato plant.
64. The transgenic plant of claim 61, wherein the transgenic plant is a tobacco plant.
65. A method of generating at least one cationic peptide, the method comprising: propagating at least one transgenic plant of claim 61; and
isolating the cationic peptide wherein the peptide is isolated from a whole plant, plant part, or intracellular organelle.
66. A method for generating at least part of a plant with increased shelf-life or storage life, the method comprising:
growing a transgenic plant of claim 61; and
harvesting at least a part of the plant, wherein the harvested plant part remains free from pathogen infection longer than a non-transgenic control.
67. The transgenic plant part of claim 66, wherein the plant part is selected from the group consisting of cut flowers, fruits, vegetables, and herbs.
68. A chimeric plant, comprising a first part and a second part, wherein the first part comprises tissue from the transgenic plant of claim 61, and the second part comprises non-transgenic plant tissue.
69. The transgenic plant of claim 61, wherein the transgenic plant displays an increased yield of plants or plant products when compared to its non-transgenic counterpart.
70. The transgenic plant of claim 61, wherein the transgenic plant displays resistance to late blight due to Phytophthora infestans.
71. The transgenic plant of claim 61, wherein the transgenic plant displays resistance to soft rot due to Erwinia carotovara.
72. The transgenic plant of claim 1, wherein the N-terminal extension is joined to the cationic peptide by a spacer peptide.
73. The transgenic plant of claim 1, wherein the N-terminal extension modulates an anti-microbial activity of the cationic peptide.
74. The transgenic plant of claim 1, wherein the N-terminal extension comprises an amino acid sequence no more than 25 amino acid residues long.
75. The transgenic plant of claim 1, wherein the N-terminal extension comprises an anionic pro-region peptide.
76. The transgenic plant of claim 75, wherein the anionic pro-region peptide is operably lined to an N-terminus of the N-terminal extension.
77. The transgenic plant of claim 75, wherein an anionic pro-region peptide is operably lined to the N-terminus of SEQ ID NO: 11.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A system to facilitate virtual tape management of data and remote backup, archival, and retrieval of said data via communication of encrypted data comprising:
a virtual tape management central processing unit;
at least one host processor central processing unit communicably attached to said virtual tape management central processing unit wherein said host includes a catalog storing tape related information;
a pair of independent RAID assemblies communicably attached to said virtual tape management central processing unit;
first software for validating authorized remote access to said RAID assemblies and for encryption of data, said first software executing from within said virtual tape management central processing unit;
second software for facilitating remote configuration and utilization of said virtual tape management central processing unit; and
third software for tape image file processing including inspecting each said RAID assembly to determine if a file is present on one or both said RAID assemblies, opening said file, reading tape related information thereon, and reading from or writing to said RAID assemblies.
2. The system to facilitate virtual tape management of data in accordance with claim 1 including a virtual tape system catalog storing tape related information, said catalog being updated on creation of a tape image, on movement of a tape image, or on instruction from a security administrator central processing unit.
3. The system to facilitate virtual tape management of data in accordance with claim 1 wherein said first software is receptive to and processes data communicated from said second software.
4. The system to facilitate virtual tape management of data in accordance with claim 1 wherein said second software is receptive to and processes data communicated from said first software.
5. The system to facilitate virtual tape management in accordance with claim 1 further comprising a communication means wherein data exchanged between said RAID assemblies and said virtual tape management central processing unit is encrypted prior to transmission and decrypted subsequent to transmission.
6. The system to facilitate virtual tape management in accordance with claim 2 further comprising a communication means wherein data exchanged between said security administrator central processing unit and said virtual tape management central processing unit is encrypted prior to transmission between said central processing units and decrypted subsequent to transmission between said central processing units.
7. A system to facilitate virtual tape management of data and remote backup, archival, and retrieval of said data via communication of encrypted data comprising:
a virtual tape management central processing unit;
a pair of remote independent RAID assemblies communicably attached to said virtual tape management central processing unit;
first software for validating authorized remote access to said disk drives and for encryption of data, said first software executing from within said virtual tape management central processing unit;
second software for facilitating remote configuration and utilization of said virtual tape management central processing unit;
at least one host processor central processing unit communicably attached to said virtual tape management central processing unit wherein said host includes a catalog storing tape related information; and
third software for data integrity for tape image files for said RAID assemblies which includes determining which files are expected to be on said RAID assemblies, enumerating tape image files on said RAID assemblies, opening said tape image files, reading said tape related information, and comparing the tape related information for data integrity.
8. A computer based method for facilitating utilization of a virtual tape management system for remote backup, archival and retrieval of data via communication of encrypted data comprising:
(a) initializing and communicating with a virtual tape management central processing unit from a host;
(b) establishing a connection between said virtual tape management central processing unit and a pair of remote disk drives;
(c) managing a virtual tape system catalog containing tape related information on each virtual tape image including volume serial number;
(d) inspecting each said disk drive for desired tape related information including volume serial number; and
(e) determining if a file is present, opening said file, reading said tape related information thereon, and reading or writing to said disk arrays.