1. A method of collecting cells, comprising the steps of:
preparing a processed-bone comprising a bone extracted from a living body, a covering agent and first and second holes, wherein an outer surface of the bone is covered with the covering agent attached to the outer surface of the bone, and the first and second holes penetrate through the covering agent and the outer surface of the bone into the interior of the bone;
introducing a liquid into the processed-bone from the first hole; and
collecting a cell-containing liquid from the second hole.
2. The method according to claim 1, wherein the first and second holes penetrate through the covering agent and through a periosteum or articular cartilage into the interior of the bone.
3. The method according to claim 1, wherein the liquid to be introduced into the processed-bone is a liquid culture medium, physiological saline, phosphate buffered saline, an organ preservation solution, plasma, serum or a mixture thereof.
4. The method according to claim 1, wherein the liquid to be introduced into the processed-bone comprises at least one selected from a drug, a cell, a nucleic acid, and a protein.
5. The method according to claim 1, wherein the steps of preparation, introduction, and collection are performed in a temperature range of from 4\xb0 C. to 50\xb0 C. both inclusive.
6. The method according to claim 1, wherein the covering agent is at least one selected from a resin, an adhesive, a polymer membrane, a gel, and gypsum.
7. The method according to claim 6, wherein the resin is a thermosetting resin, a thermoplastic resin or a photo-curable resin.
8. The method according to claim 7, wherein the resin is at least one selected from a silicone resin, an epoxy resin, a phenol resin, and polymethyl methacrylate.
9. The method according to claim 6, wherein the polymer membrane is at least one selected from a biopolymer membrane and a synthetic polymer membrane.
10. The method according to claim 9, wherein the biopolymer membrane contains at least one polysaccharide selected from chitosan, an alginate, and pectin.
11. The method according to claim 6, wherein the adhesive is an inorganic adhesive, a natural adhesive or a synthetic adhesive.
12. The method according to claim 1, wherein the covering agent is attached to the bone at a temperature of from 4\xb0 C. to 50\xb0 C. both inclusive.
13. A method of collecting cells, comprising the steps of:
preparing a processed-bone by covering an outer surface of a bone extracted from a living body with a covering agent; and forming first and second holes that penetrate through the covering agent and the outer surface of the bone into the interior of the bone;
introducing a liquid into the processed-bone from the first hole; and
collecting a cell-containing liquid from the second hole.
14. The method according to claim 13, wherein the first and second holes penetrate through the covering agent and through a periosteum or articular cartilage into the interior of the bone.
15. The method according to claim 13, wherein the liquid to be introduced into the processed-bone is a liquid culture medium, physiological saline, phosphate buffered saline, an organ preservation solution, plasma, serum or a mixture thereof.
16. The method according to claim 13, wherein the covering agent is at least one selected from a resin, an adhesive, a polymer membrane, a gel, and gypsum.
17. A method of collecting cells, comprising the steps of:
preparing a processed-bone by forming first and second holes that penetrate through an outer surface of a bone extracted from a living body into the interior of the bone; and covering the outer surface of the bone with the covering agent;
introducing a liquid into the processed-bone from the first hole; and
collecting a cell-containing liquid from the second hole.
18. The method according to claim 17, wherein the first and second holes penetrate through the covering agent and through a periosteum or articular cartilage into the interior of the bone.
19. The method according to claim 17, wherein the liquid to be introduced into the processed-bone is a liquid culture medium, physiological saline, phosphate buffered saline, an organ preservation solution, plasma, serum or a mixture thereof.
20. The method according to claim 17, wherein the covering agent is at least one selected from a resin, an adhesive, a polymer membrane, a gel, and gypsum.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A sanitary napkin comprising:
a topsheet;
a backsheet;
an absorbent core disposed between the topsheet and backsheet; and
a skin care composition applied on the topsheet;
wherein the topsheet comprises a first layer comprising a nonwoven, a second layer adjacent the first layer, and a plurality of fiber tufts comprising integral extensions of fibers from the nonwoven that extend into andor through the second layer; and wherein the first layer and second layer are joined by adhesive, ultrasonic bonding, interlocking mechanical engagement, andor combinations thereof.
2. The sanitary napkin of claim 1, wherein said caliper is less than about 2.0 mm.
3. The sanitary napkin of claim 1, wherein said sanitary napkin comprises a topsheet, and said skin care composition is applied to said topsheet on an area at least one square centimeter in an amount of 0.0015 mgcm2 to 15.5 mgcm2.
4. The sanitary napkin of claim 1, wherein said sanitary napkin comprises a topsheet, and said skin care composition is applied to said topsheet in an amount of 0.02 mgcm2 to 7.75 mgcm2.
5. The sanitary napkin of claim 1, wherein said skin care composition comprises an antimicrobial agent.
6. The sanitary napkin of claim 1 wherein said composition comprises 60% to 99.9% by weight of the carrier wherein the carrier is selected from the group consisting of petroleum-based hydrocarbons having 4 to 32 carbon atoms, fatty alcohols having 12 to 24 carbon atoms, lower alcohols having 1 to 6 carbon atoms, low molecular weight glycols and polyols, lanolin, and mixtures thereof.
7. The sanitary napkin of claim 1 wherein the skin care composition further comprises 0.001% to 10% by weight of a skin conditioning agent selected from the group consisting of panthenol, glycerine, and mixtures thereof.
8. The sanitary napkin of claim 1 wherein the composition further comprises a skin treatment active selected from the group consisting of allantoin, aluminum hydroxide gel, calamine, cysteine hydrochloride, racemic methionine, sodium bicarbonate, Vitamin C and derivatives thereof, serine protease, metalloprotease, cysteine protease, aspartyl protease, peptidase, phenylsulfonyl fluoride, lipase, diesterase, urease, amylase, elastase, nuclease, guanidinobenzoic acid and its salts and derivatives, chamomile, and mixtures thereof.
9. A method of reducing skin disorders comprising the steps of:
a. wearing an absorbent article having a caliper of less than 5 mm; wherein the absorbent article comprises a topsheet having a first layer, a second layer, and integral fiber tufts extending from the first layer and through the second layer, at least some of the fiber tufts comprising a skin care composition; and
b. transferring at least a portion of the skin care composition to an external skin surface via movement of the fiber tufts against the skin.
10. The method of claim 9 wherein the absorbent article comprises 0.0015 mgcm2 to 15.5 mgcm2 of the skin care composition and wherein 0.00045 mgcm2 to 124 mgcm2 of the skin care composition is transferred onto the external skin surface within a 24 hour period.
11. A sanitary napkin for wearing adjacent the pudendal region, said sanitary napkin comprising a topsheet, a backsheet joined to the topsheet, wings, and an absorbent core disposed between the topsheet and backsheet, said topsheet comprising a first layer, a second layer, and at least one integral discrete fiber tuft, wherein said sanitary napkin comprises a skin care composition, wherein said sanitary napkin has a caliper less than 5.0 mm, and wherein the at least one integral discrete fiber tuft extends from within the first layer and through the second layer of the topsheet.
12. The sanitary napkin of claim 11, wherein said tuft extends out-of-plane in the Z-direction with respect to the sanitary napkin.
13. The sanitary napkin of claim 11, wherein said caliper is less than 2.0 mm.
14. The sanitary napkin of claim 11, wherein said skin care composition is applied to said topsheet in an amount of 0.0015 mgcm2 to 15.5 mgcm2.
15. The sanitary napkin of claim 1, wherein said caliper is less than about 3.0 mm.
16. The sanitary napkin of claim 1, wherein the skin care composition is hydrophobic.
17. The sanitary napkin of claim 1, wherein the topsheet second layer comprises a film.
18. The sanitary napkin of claim 1, wherein the topsheet second layer comprises a second nonwoven.
19. The sanitary napkin of claim 1, wherein the skin care composition comprises at least one of hexamidine, zinc oxide, and niacinamide.
20. The sanitary napkin of claim 1, wherein the skin care composition comprises:
a. 0.001% to 0.1% by weight of hexamidine;
b. 0.001% to 10% by weight of zinc oxide;
c. 0.01% to 10% by weight of niacinamide; and
a carrier.