1. An image-capturing apparatus comprising:
an image-capturing unit shooting a subject to generate an image thereof;
a recording unit recording thereon one or more of color reproduction matrices in association with each of a plurality of kinds of illuminants including natural light and at least one kind of artificial illumination device;
a white balance gain calculating unit calculating an evaluated value relating to a white balance gain to be applied to the image;
a computing unit selecting at least two of the color reproduction matrices which are associated with different kinds of illuminants from the color reproduction matrices recorded on said recording unit based on the evaluated value, and computing a correction color reproduction matrix based on at least two of the color reproduction matrices being selected by interpolation processing; and
a color reproduction processing unit applying color reproduction processing to the image generated by said image-capturing unit, by using the corrected color reproduction matrix,
wherein said computing unit expresses the kinds of illuminants associated with the color reproduction matrices recorded on said recording unit as loci in system of coordinates in a color space, and computes the corrected color reproduction matrix through interpolation processing by determining a weighted average of the color reproduction matrices based on position coordinates in the system of coordinates.
2. The image-capturing apparatus according to claim 1, wherein:
said recording unit records two or more of the color reproduction matrices in association with at least one kind of illuminant of the plurality of kinds of illuminants including natural light and at least one kind of artificial illumination device; and
said computing unit computes the corrected color reproduction matrix by interpolation processing according to at least two of the color reproduction matrices and to at least one of the color reproduction matrices among the color reproduction matrices recorded on said recording unit, the at least two of the color reproduction matrices being associated with the at least one kind of illuminant, the at least one of the color reproduction matrices being associated with an illuminant of a different kind from that of the at least one kind of illuminant.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26.
2. The monoclonal antibody of claim 1 wherein said antibody is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303, and 108-394.
3. A hybridoma cell line which secretes a monoclonal antibody which binds to a shared epitope Human Immunodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26.
4. The hybridoma cell line of claim 3, wherein said cell line is selected from the group consisting of A.T.C.C. Deposit No. HB ______ , A.T.C.C. Deposit No. HB ______ , A.T.C.C. Deposit No. HB ______ , A.T.C.C. Deposit No. HB ______ , A.T.C.C. Deposit No. HB ______ , and A.T.C.C. Deposit No. HB ______ .
5. A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
a) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26 for a time and under conditions sufficient for the formation of antibodyantigen complexes; and
b) detecting said complexes, presence of said complexes indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in said test sample.
6. The method of claim 5 wherein said at least one monoclonal antibody of step (a) is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303 and 108-394.
7. The method of claim 6 wherein said at least one monoclonal antibody of step (a) is labeled.
8. A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
a) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein 24 and Human Immunodeficiency Virus-2 protein p26 for a time and under conditions sufficient for the formation of antibodyantigen complexes;
b) adding a conjugate to the resulting antibodyantigen complexes for a time and under conditions sufficient to allow said conjugate to bind to the bound antigen, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal; and
c) detecting presence of antigen which may be present in said test sample by detecting a signal generated by said signal generating compound, presence of said signal indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen in said test sample.
9. The method of claim 8 wherein said at least one monoclonal antibody of step (a) is selected from the group consisting of 120A-270, 115B-151 117-289, 103-350, 115B-303, and 108-394.
10. The method of claim 8 wherein said antibody of step (b) of said conjugate is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303, and 108-394.
11. The method of claim 8 wherein said at least one monoclonal antibody of step (a) is selected from the group consisting of 120-270, 108-394 and 115B-303, and said antibody of step (b) of said conjugate is selected from the group consisting of 117-289 and 115B-151.
12. The method of claim 11 wherein said at least one monoclonal antibody of step (a) is 120A-270 and said antibody of step (b) of said conjugate is 115B-151.
13. A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
(a) contacting: 1) at least one monoclonal antibody which binds to a shared epitope of HIV-1 p24 antigen and HIV-2 p26 antigen bound to a solid support, 2) said test sample, and 3) an indicator reagent comprising an antibody which binds to HIV-1 antigen and HIV-2 antigen to which a signal generating compound is attached, to form a mixture;
(b) incubating said mixture for a time and under conditions sufficient to form antibodyantigenantibody complexes;
(c) detecting presence of a measurable signal generating by said signal-generating compound, presence of said signal indicating presence of one or more antigens in said test sample selected from the group consisting of HIV-1 antigen and HIV-2 antigen.
14. The method of claim 13 wherein said at least one monoclonal antibody of step (a) is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303 and 108-394.
15. The method of claim 13 wherein said antibody of said indicator reagent of step (a) is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303 and 108-394.
16. The method of claim 13 wherein said at least one monoclonal antibody of step (a) is 120A-270 and said antibody of said indicator reagent of of step (a) is 115B-151.
17. A kit for determining the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen in a test sample comprising: (a) at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26; and (b) a conjugate comprising an antibody attached to a signal generating compound capable of generating a detectable signal.
18. The kit of claim 17 wherein said at least one monoclonal antibody of (a) is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-303 and 108-394.
19. The kit of claim 17 wherein said antibody of (b) is selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 115B-3-3 and 108-394.
20. A diagnostic reagent comprising at least one monoclonal antibody selected from the group consisting of 120A-270, 115B-151, 117-289, 103-350, 108-394 and 115B-303.
21. An isolated peptide comprising the amino acid sequence of SEQ ID NO:1.
22. An isolated peptide comprising the amino acid sequence of SEQ ID NO:2.
23. An isolated peptide comprising the amino acid sequence of SEQ ID NO:3.
24. An isolated peptide comprising the amino acid sequence of SEQ ID NO:4.
25. An isolated peptide comprising the amino acid sequence of SEQ ID NO:5.
26. An isolated peptide comprising the amino acid sequence of SEQ ID NO:6.
27. A method of detecting 1) one or more antibodies selected from the group consisting of HIV-1 antibody and HIV-2 antibody, and 2) one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing said one or more of said antibodies and one or more of said antigens, comprising the steps of:
a) contacting said test sample with at least one HIV-1 antigen which binds to HIV-1 antibody for a time and under conditions sufficient for the formation of HIV-1 antigenHIV-1 antibody complexes;
b) detecting said HIV-1 antigenHIV-1 antibody complexes, presence of said complexes indicating presence of HIV-1 antibody in said test sample;
c) contacting said test sample with at least one HIV-2 antigen which binds to HIV-2 antibody for a time and under conditions sufficient for the formation of HIV-2 antigenHIV-2 antibody complexes;
d) detecting said HIV-2 antigenHIV-2 antibody complexes, presence of said complexes indicating presence of HIV-2 antibody in said test sample;
e) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26 for a time and under conditions sufficient for the formation of antibodyantigen complexes; and
f) detecting said complexes, presence of said complexes indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in said test sample.
28. A method of detecting 1) one or more antibodies selected from the group consisting of HIV-1 antibody and HIV-2 antibody, and 2) one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing said one or more of said antibodies and one or more of said antigens, comprising the steps of:
a) contacting said test sample with at least one HIV-1 antigen which binds to HIV-1 antibody for a time and under conditions sufficient for the formation of HIV-1 antigenHIV-1 antibody complexes:
b) adding a conjugate to the resulting HIV-1 antigenHIV-1 antibody complexes for a time and under conditions sufficient to allow said conjugate to bind to the bound antibody, wherein said conjugate comprises an antigen attached to a signal generating compound capable of generating a detectable signal;
c) detecting HIV-1 antibody which may be present in said test sample by detecting a signal generated by said signal generating compound, presence of said signal indicating presence of HIV-1 antibody in said test sample;
d) contacting said test sample with at least one HIV-2 antigen which binds to HIV-2 antibody for a time and under conditions sufficient for the formation of HIV-2 antigenHIV-2 antibody complexes:
e) adding a conjugate to the resulting HIV-2 antigenHIV-2 antibody complexes for a time and under conditions sufficient to allow said conjugate to bind to the bound antibody, wherein said conjugate comprises an antigen attached to a signal generating compound capable of generating a detectable signal;
f) detecting HIV-2 antibody which may be present in said test sample by detecting a signal generated by said signal-generating compound, presence of said signal indicating presence of HIV-2 antibody in said test sample;
g) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein 24 and Human Immunodeficiency Virus-2 protein p26 for a time and under conditions sufficient for the formation of antibodyantigen complexes;
h) adding a conjugate to the resulting antibodyantigen complexes for a time and under conditions sufficient to allow said conjugate to bind to the bound antigen, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal; and
i) detecting presence of antigen which may be present in said test sample by detecting a signal generated by said signal generating compound, presence of said signal indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen in said test sample.